Eviction-driven infanticide and sexually selected adoption and infanticide in a neotropical parrot.

Infanticide and adoption have been attributed to sexual selection, where an individual later reproduces with the parent whose offspring it killed or adopted. While sexually selected infanticide is well known, evidence for sexually selected adoption is anecdotal. We report on both behaviors at 346 nests over 27 y in green-rumped parrotlets (Forpus passerinus) in Venezuela. Parrotlets are monogamous with long-term pair bonds, exhibit a strongly male-biased adult sex ratio, and nest in cavities that are in short supply, creating intense competition for nest sites and mates. Infanticide attacks occurred at 256 nests in two distinct contexts: 1) Attacks were primarily committed by nonbreeding pairs (69%) attempting to evict parents from the cavity. Infanticide attacks per nest were positively correlated with population size and evicting pairs never adopted abandoned offspring. Competition for limited nest sites was a primary cause of eviction-driven infanticide, and 2) attacks occurred less frequently at nests where one mate died (31%), was perpetrated primarily by stepparents of both sexes, and was independent of population size. Thus, within a single species and mating system, infanticide occurred in multiple contexts due to multiple drivers. Nevertheless, 48% of stepparents of both sexes adopted offspring, and another 23% of stepfathers exhibited both infanticide and long-term care. Stepfathers were often young males who subsequently nested with widows, reaching earlier ages of first breeding than competitors and demonstrating sexually selected adoption. Adoption and infanticide conferred similar fitness benefits to stepfathers and appeared to be equivalent strategies driven by limited breeding opportunities, male-biased sex ratios, and long-term monogamy.

Association of S(+) ketamine, dexmedetomidine and butorphanol for chemical restraint in scarlet macaws (Ara macao) / Associação cetamina S(+), dexmedetomidina e butorfanol na contenção química de araracangas (Ara macao)

The present study aimed at assessing the effects of combining 20 mg/kg S(+) ketamine with 25 µg/kg dexmedetomidine and 0.4 mg/kg butorphanol on the physiological parameters and anesthetic recovery time and score of eight captive scarlet macaw (Ara macao) specimens. These specimens were captured at the Marabá Zoobotanic Foundation (Fundação Zoobotânica de Marabá), Pará, using butterfly and mist nets, and subsequently subjected to the proposed protocol. The following physiological parameters were evaluated: heart rate (HR), respiratory rate (RR), saturation of peripheral oxygen (SpO2), body temperature (BT), and non-invasive blood pressure 5 min after drug administration (M0) and every 10 min thereafter (M1‒M5), with a total of 55 min of analysis of anesthetic effects. Glycemia was measured 5 min after drug administration and every 30 min thereafter. Anesthetic induction and recovery times were also determined. Among the parameters evaluated in this study, both HR and BT significantly decreased throughout the anesthetic period, with the lowest levels at 55 min after drug administration (M5). In contrast, RR did not significantly differ, and all animals remained stable, maintaining an RR close to a mean of 20 ± 8 cpm. Throughout the anesthetic period, SpO2was 92 ± 5%, with no significant difference. The birds remained under spontaneous ventilation and without oxygen supplementation. Systolic, diastolic, and mean blood pressures remained stable, with no significant differences in any of these measurements. At M0 and M3, the glycemia decreased slightly, albeit with no significant difference justifying an adverse effect or even hypoglycemia. The anesthetic induction time, from M0 to decubitus, was 2.4 ± 0.7 min. The anesthetic recovery time, from M0 to effortless bipedal position and adequate phalangeal flexion, was 99.3 ± 32.4 min. The sedation was assessed as intense, and the anesthetic recovery was rated excellent in 62.5% and good in 37.5% of the animals.(AU)

O presente estudo objetivou avaliar os efeitos do uso da cetamina S(+) 20 mg/kg associada à dexmedetomidina 25 µg/kg e butorfanol 0,4 mg/kg sobre os parâmetros fisiológicos, tempo e qualidade da recuperação anestésica de araracangas (Ara macao). Foram utilizados oito espécimes de Ara macao cativas da Fundação Zoobotânica de Marabá, Pará. A captura foi realizada com o uso de puçá e rede de contenção e em seguida as aves foram submetidas ao protocolo proposto. Foram avaliados: frequência cardíaca, frequência respiratória, saturação parcial da oxihemoglobina (SpO2), temperatura corporal e pressão arterial não-invasiva a partir de 5 minutos após a aplicação dos fármacos (M0) e a cada 10 minutos seguintes (M1, M2, M3, M4 e M5), totalizando 55 minutos de contemplação dos efeitos anestésicos. A glicemia foi avaliada aos 5 minutos da aplicação dos fármacos e repetida após 30 minutos. Também foi determinado o tempo de indução e de recuperação. Dentre os parâmetros avaliados, a frequência cardíaca e a temperatura demonstraram queda estatisticamente significativa ao longo do período anestésico, ambas com os menores valores registrados aos 55 minutos após a aplicação dos fármacos (M5). A frequência respiratória não apresentou diferença estatística e todos os animais se mantiveram estáveis e com a frequência próxima a média de 20±8mpm. A saturação da oxihemoblobina (SpO2) ao longo do período anestésico foi de 92±5%, não houve diferença estatisticamente relevante, as aves permaneceram sob ventilação espontânea e sem suplementação de oxigênio. As pressões arteriais sistólica, diastólica e média, mantiveram-se estáveis e não houve diferença estatística para nenhuma dessas medidas. A glicemia, mensurada em M0 e M3 demonstrou queda discreta, sem diferença significativa capaz de justificar um efeito adverso ou mesmo hipoglicemia. O tempo de indução, desde aplicação dos anestésicos até o decúbito, foi de 2,4±0,7 minutos. O tempo de recuperação, compreendido desde a aplicação dos fármacos (M0) até a constatação da posição bipedal sem esforço e adequada flexão das falanges, foi de 99,3±32,4 minutos. A qualidade de sedação foi considerada intensa e a recuperação anestésica foi classificada como ótima para 62,5% e boa para 37,5% dos animais.(AU)

Fine-scale genetic structure in the critically endangered red-fronted macaw in the absence of geographic and ecological barriers.

Behavioural and socio-cultural traits are recognized in the restriction of gene flow in species with high cognitive capacity and complex societies. This isolation by social barriers has been generally overlooked in threatened species by assuming disrupted gene flow due to population fragmentation and decline. We examine the genetic structure and ecology of the global population of the Critically Endangered red-fronted macaw (Ara rubrogenys), an endemic species to the inter-Andean valleys of Bolivia. We found a fine-scale genetic structuring in four genetic clusters. Genetic diversity was higher in wild compared to captive-bred macaws, but similar to that of captive wild-caught macaws. We found no clear evidence of severe genetic erosion in the population in recent decades, but it was patent in historic times, overlapping with drastic human habitat transformation and macaw persecution over millennia. We found no evidence of geographical and ecological barriers, owing to the high dispersal ability, nesting and foraging habits between genetic clusters. The lack of genetic intermixing despite long-distance foraging and seasonal movements suggests recruitment in natal colonies and other social factors reinforcing philopatry-related genetic structure. Conservation efforts should be specifically focussed on major threats in each genetic cluster as independent conservation units, and also considered in ex-situ management.

Seven Years of Parrot Conservation in La Moskitia, Honduras.

The population of the Central American scarlet macaw ( Ara macao cyanoptera) and other parrots have and continue to decline throughout the region largely from poaching to supply the illegal wildlife trade. Conservation efforts address this issue through protection efforts, including community patrols and government engagement, as well as nest monitoring, rescue and release operations, capacity building, and education and consciousness raising programs, all of which rely on long-lasting relationships and commitment. Using these principles, in 2010, a coalition of people came together, including the authors, to build a conservation program against overwhelming odds and with surprising success.

Network structure embracing mutualism-antagonism continuums increases community robustness.

Theory predicts that contrasting properties of mutualistic and antagonistic networks differentially promote community resilience to species loss. However, the outcome of most ecological interactions falls within a continuum between mutualism and antagonism, and we ignore the extent to which this interactions' continuum might influence community stability. Using a large data set of interactions, we compared co-extinction cascades that either consider or ignore the mix of beneficial and detrimental actions that parrots exert on plants. When the antagonism-mutualism continuum was considered, a combination of the properties that separately enhance community stability in ecological networks emerged. This combination of properties led to an overall increase of the parrot community robustness to face plant species loss. Our results highlight that the conditional outcomes of interactions can influence the structure of ecological networks, thus affecting our predictions of community stability against eventual changes.

Skeletogenesis of Myiopsitta monachus (Psittaciformes) and sequence heterochronies in Aves.

The ossification sequence of Myiopsitta monachus was determined. Myiopsitta has a similar sequence to other altricial birds, with delayed skeletons compared to precocial species. The hindlimbs ossify before the forelimbs, a condition that could be linked to altriciality. To determine the stability of the sequences of ossification across birds, we selected species of different groups of Aves and used event-pairing method and character mapping on a phylogeny. Our results show that the homogeneity in the development of birds was supported by 56.77% of the character states. Event-pair cracking phylogenetic method was applied to identify sequence heterochronies. Results reveal a high number of heterochronies and show that the long bones in limbs may behave as modules. In Myiopsitta, the ossa ectethmoidale and mesethmoidale ossify early. These bones provide the origin site of the Psittaciformes' novel adductor m. ethmomandibularis, associated with strong bite forces, and its acceleration in the sequence may correspond to the functional hypothesis. Also, the early appearance of some hyoid apparatus elements occurs, and could be related to the development of tongue in Psittaciformes and its role in handling food, and is in concordance with the functional and size hypothesis.

Endangered plant-parrot mutualisms: seed tolerance to predation makes parrots pervasive dispersers of the Parana pine.

Parrots are largely considered plant antagonists as they usually destroy the seeds they feed on. However, there is evidence that parrots may also act as seed dispersers. We evaluated the dual role of parrots as predators and dispersers of the Critically Endangered Parana pine (Araucaria angustifolia). Eight of nine parrot species predated seeds from 48% of 526 Parana pines surveyed. Observations of the commonest parrot indicated that 22.5% of the picked seeds were dispersed by carrying them in their beaks. Another five parrot species dispersed seeds, at an estimated average distance of c. 250 m. Dispersal distances did not differ from those observed in jays, considered the main avian dispersers. Contrary to jays, parrots often dropped partially eaten seeds. Most of these seeds were handled by parrots, and the proportion of partially eaten seeds that germinated was higher than that of undamaged seeds. This may be explained by a predator satiation effect, suggesting that the large seeds of the Parana pine evolved to attract consumers for dispersal. This represents a thus far overlooked key plant-parrot mutualism, in which both components are threatened with extinction. The interaction is becoming locally extinct long before the global extinction of the species involved.

Anestesia por perfusão de saco aéreo em arara (Ara chloropterus): relato de caso / Anesthesia by infusion of air sac in a macaw (Ara chloropterus): a case report

O presente relato descreve a utilização da técnica anestésica deperfusão em saco aéreo durante a cirurgia para correção do bicode uma arara (Ara chloropterus). Esse tipo de abordagem anestésicaé recomendado para procedimentos em que se faz necessáriaa manipulação da região de cabeça e pescoço das aves. Após contençãofísica do animal, a anestesia foi induzida com auxílio demáscara facial, conectada ao sistema de Maplesson D (Baraka),utilizando isofluorano e fluxo diluente de oxigênio de 1L/min. Opreparo da cânula e posterior introdução em saco aéreo torácicocaudal foi estabelecido de modo similar ao descrito por Gunkele Lafortune (2005). A técnica mostrou-se adequada e bastantesegura; contudo, para ser empregada, é necessário que o profissionalanestesista conheça a anatomia e fisiologia das aves.(AU)

This report describes the use of an anesthetic perfusiontechnique applied in the air sac of a Macaw (Ara chloropterus)during surgery for correction of its broken beak. This anestheticapproach is recommended for procedures in which manipulationof the avian head or neck is necessary. After appropriateimmobilization of the animal, the anesthetic is introduced withthe help of a face mask connected to the Mapleson D (Baraka)system, using isoflurane with 1L-min. flow of diluent oxygen.The cannula preparation and introduction into the caudalthoracic air sac was performed is a way similar to that describedby Gunkel and Lafortune (2005). The technique proved to beappropriate and very safe, but the anesthetic professional musthave thorough of avian anatomy and physiology.(AU)

Anestesia por perfusão de saco aéreo em arara (Ara chloropterus): relato de caso / Anesthesia by infusion of air sac in a macaw (Ara chloropterus): a case report

O presente relato descreve a utilização da técnica anestésica deperfusão em saco aéreo durante a cirurgia para correção do bicode uma arara (Ara chloropterus). Esse tipo de abordagem anestésicaé recomendado para procedimentos em que se faz necessáriaa manipulação da região de cabeça e pescoço das aves. Após contençãofísica do animal, a anestesia foi induzida com auxílio demáscara facial, conectada ao sistema de Maplesson D (Baraka),utilizando isofluorano e fluxo diluente de oxigênio de 1L/min. Opreparo da cânula e posterior introdução em saco aéreo torácicocaudal foi estabelecido de modo similar ao descrito por Gunkele Lafortune (2005). A técnica mostrou-se adequada e bastantesegura; contudo, para ser empregada, é necessário que o profissionalanestesista conheça a anatomia e fisiologia das aves.

This report describes the use of an anesthetic perfusiontechnique applied in the air sac of a Macaw (Ara chloropterus)during surgery for correction of its broken beak. This anestheticapproach is recommended for procedures in which manipulationof the avian head or neck is necessary. After appropriateimmobilization of the animal, the anesthetic is introduced withthe help of a face mask connected to the Mapleson D (Baraka)system, using isoflurane with 1L-min. flow of diluent oxygen.The cannula preparation and introduction into the caudalthoracic air sac was performed is a way similar to that describedby Gunkel and Lafortune (2005). The technique proved to beappropriate and very safe, but the anesthetic professional musthave thorough of avian anatomy and physiology.

Seed dispersal and predation of Buchenavia tomentosa Eichler (Combretaceae) in a Cerrado sensu stricto, midwest Brazil.

The ecology of seed dispersal is critical to understand the patterns of distribution and abundance of plant species. We investigated seed dispersal aspects associated with the high abundance of Buchenavia tomentosa in the Serra Azul State Park (PESA). We estimated fruit production and conducted fruit removal experiments. We carried out diurnal and nocturnal observations on frugivory as well as germination tests. Fruiting occurred in the dry season and totaled 1,365,015 ± 762,670 fruits.ha-1. B. tomentosa fruits were utilized by eight animal species. The lowland tapir (Tapirus terrestris) was considered the main seed disperser. Leafcutter ants (Atta laevigata and Atta sexdens) participated in the seed cleaning and occasionally dispersed seeds. The beetle Amblycerus insuturatus, blue-and-yellow macaw (Ara ararauna) and red-and-green macaw (Ara chloropterus) were considered pre-dispersal seed predators. The seeds manually cleaned presented higher germination rate (100%) and speed index (4.2 seeds.d-1) than that of seeds with pulp. Germination of seeds found in tapirs'feces was 40%, while for the seeds without pulp it was 25%. The high abundance of B. tomentosa in the cerrado of PESA may be due to massive fruit production, low rates of seed predation, and efficient seed dispersal by tapirs, occurring before the rains which promote germination and recruitment of this species.

Seed dispersal and predation of Buchenavia tomentosa Eichler (Combretaceae) in a Cerrado sensu stricto, midwest Brazil / Dispersão e predação de sementes de Buchenavia tomentosa Eichler (Combretaceae) em Cerrado sentido restrito, centro-oeste do Brasil

The ecology of seed dispersal is critical to understand the patterns of distribution and abundance of plant species. We investigated seed dispersal aspects associated with the high abundance of Buchenavia tomentosa in the Serra Azul State Park (PESA). We estimated fruit production and conducted fruit removal experiments. We carried out diurnal and nocturnal observations on frugivory as well as germination tests. Fruiting occurred in the dry season and totaled 1,365,015 ± 762,670 fruits.ha–1. B. tomentosa fruits were utilized by eight animal species. The lowland tapir (Tapirus terrestris) was considered the main seed disperser. Leafcutter ants (Atta laevigata and Atta sexdens) participated in the seed cleaning and occasionally dispersed seeds. The beetle Amblycerus insuturatus, blue-and-yellow macaw (Ara ararauna) and red-and-green macaw (Ara chloropterus) were considered pre-dispersal seed predators. The seeds manually cleaned presented higher germination rate (100%) and speed index (4.2 seeds.d–1) than that of seeds with pulp. Germination of seeds found in tapirsfeces was 40%, while for the seeds without pulp it was 25%. The high abundance of B. tomentosa in the cerrado of PESA may be due to massive fruit production, low rates of seed predation, and efficient seed dispersal by tapirs, occurring before the rains which promote germination and recruitment of this species.(AU)

A ecologia da dispersão de sementes é importante para entender a distribuição e abundância das espécies vegetais. Investigamos os fatores relacionados à elevada abundância de B. tomentosa no Parque Estadual da Serra Azul (PESA) avaliando aspectos da dispersão de sementes. Estimamos a produção de frutos e realizamos experimentos de remoção de frutos. Fizemos observações diurnas e noturnas sobre a frugivoria e testamos a germinação de sementes. A frutificação ocorreu na estação seca totalizando 1.365.015 ± 762.670 frutos.ha–1. Oito espécies de animais utilizaram frutos de B. tomentosa. Anta (Tapirus terrestris) foi considerada o principal dispersor. Saúvas (Atta laevigata e Atta sexdens) participaram na limpeza das sementes e como dispersores ocasionais. O besouro Amblycerus insuturatus, arara-canindé (Ara ararauna) e arara-vermelha (Ara chloropterus) foram considerados predadores pré-dispersão. Sementes despolpadas manualmente tiveram maior percentual (100%) e velocidade de geminação (4,2 sementes.dia–1) que aquelas com polpa. Sementes encontradas nas fezes de antas apresentaram 40,0% de germinação e sementes sem polpa 25,0%. A massiva produção de frutos, baixa taxa de predação de sementes, além da eficiente dispersão por antas, antes da estação chuvosa, favorecem o recrutamento e são fatores que contribuem de forma decisiva para a elevada abundância de B. tomentosa observada no cerrado do PESA.(AU)

Seed dispersal and predation of Buchenavia tomentosa Eichler (Combretaceae) in a Cerrado sensu stricto, midwest Brazil / Dispersão e predação de sementes de Buchenavia tomentosa Eichler (Combretaceae) em Cerrado sentido restrito, centro-oeste do Brasil

Abstract The ecology of seed dispersal is critical to understand the patterns of distribution and abundance of plant species. We investigated seed dispersal aspects associated with the high abundance of Buchenavia tomentosa in the Serra Azul State Park (PESA). We estimated fruit production and conducted fruit removal experiments. We carried out diurnal and nocturnal observations on frugivory as well as germination tests. Fruiting occurred in the dry season and totaled 1,365,015 ± 762,670 fruits.ha–1. B. tomentosa fruits were utilized by eight animal species. The lowland tapir (Tapirus terrestris) was considered the main seed disperser. Leafcutter ants (Atta laevigata and Atta sexdens) participated in the seed cleaning and occasionally dispersed seeds. The beetle Amblycerus insuturatus, blue-and-yellow macaw (Ara ararauna) and red-and-green macaw (Ara chloropterus) were considered pre-dispersal seed predators. The seeds manually cleaned presented higher germination rate (100%) and speed index (4.2 seeds.d–1) than that of seeds with pulp. Germination of seeds found in tapirs’feces was 40%, while for the seeds without pulp it was 25%. The high abundance of B. tomentosa in the cerrado of PESA may be due to massive fruit production, low rates of seed predation, and efficient seed dispersal by tapirs, occurring before the rains which promote germination and recruitment of this species.

Resumo A ecologia da dispersão de sementes é importante para entender a distribuição e abundância das espécies vegetais. Investigamos os fatores relacionados à elevada abundância de B. tomentosa no Parque Estadual da Serra Azul (PESA) avaliando aspectos da dispersão de sementes. Estimamos a produção de frutos e realizamos experimentos de remoção de frutos. Fizemos observações diurnas e noturnas sobre a frugivoria e testamos a germinação de sementes. A frutificação ocorreu na estação seca totalizando 1.365.015 ± 762.670 frutos.ha–1. Oito espécies de animais utilizaram frutos de B. tomentosa. Anta (Tapirus terrestris) foi considerada o principal dispersor. Saúvas (Atta laevigata e Atta sexdens) participaram na limpeza das sementes e como dispersores ocasionais. O besouro Amblycerus insuturatus, arara-canindé (Ara ararauna) e arara-vermelha (Ara chloropterus) foram considerados predadores pré-dispersão. Sementes despolpadas manualmente tiveram maior percentual (100%) e velocidade de geminação (4,2 sementes.dia–1) que aquelas com polpa. Sementes encontradas nas fezes de antas apresentaram 40,0% de germinação e sementes sem polpa 25,0%. A massiva produção de frutos, baixa taxa de predação de sementes, além da eficiente dispersão por antas, antes da estação chuvosa, favorecem o recrutamento e são fatores que contribuem de forma decisiva para a elevada abundância de B. tomentosa observada no cerrado do PESA.

Avaliação de esfregaços corados pelo gram e prevalência de endoparasitas em araras de vida-livre / Evaluation of fecal gram stains and prevalence of endoparasites in free-living macaws

Although Brazil has a great bird diversity, information on the health status of free living psittacine is scarce. The aim of this study was to determine the prevalence of endoparasites and the ratio of gram-negative versus gram-positive bacteria in the feces of free-living adult Hyacinth and Green-winged macaws. Fecal samples were collected placing camouflaged sheet covers on bird foraging areas. Fifty-five swabs were evaluated by gram staining technique and 101 fecal samples were analyzed using flotation and centrifugation methods. None of the samples presented parasites or ova. The presence of gram-negative bacteria was detected in 1.8% of the fecal samples. These results suggest that parasites and gram-negative bacteria may be transient in the gastrointestinal tract of healthy free-living psittacine birds. This situation is quite different from what is observed for birds in captivity, where stressful events and improper husbandry facilitate the dysbiosis and high parasite loads, with an imbalance in the host-parasite relationship.(AU)

Embora o Brasil tenha uma grande diversidade de aves, as informações sobre o estado sanitário de psitacídeos de vida livre são escassas. O objetivo deste trabalho foi determinar a prevalência de endoparasitas e a relação entre bactérias gram-negativas e gram-positivas nas fezes de Arara-azuis e Araras-vermelhas-grandes adultas em vida-livre. Amostras de fezes foram coletadas empregando-se lonas camufladas onde as aves se reuniam para forragear. Cinquenta e cinco esfregaços foram avaliados pela técnica de gram e 101 amostras de fezes foram analisadas pelos métodos de flutuação e centrífugo-flutuação.  Nenhuma amostra foi positiva para a presença de ovos ou formas adultas de parasitas. A presença de bactérias gram-negativas foi detectada em 1.8% das amostras fecais. Esses resultados sugerem que parasitas e bactérias gram-negativas podem ser apenas transientes no trato gastrointestinal de psitacídeos saudáveis de vida livre. Essa situação é bastante diversa do que ocorre em cativeiro, onde situações de estresse e manejo inadequado facilitam a disbiose e o aumento das cargas parasitárias, com um desequilíbrio na relação hospedeiro-parasita.(AU)

Avaliação de esfregaços corados pelo gram e prevalência de endoparasitas em araras de vida-livre / Evaluation of fecal gram stains and prevalence of endoparasites in free-living macaws

Although Brazil has a great bird diversity, information on the health status of free living psittacine is scarce. The aim of this study was to determine the prevalence of endoparasites and the ratio of gram-negative versus gram-positive bacteria in the feces of free-living adult Hyacinth and Green-winged macaws. Fecal samples were collected placing camouflaged sheet covers on bird foraging areas. Fifty-five swabs were evaluated by gram staining technique and 101 fecal samples were analyzed using flotation and centrifugation methods. None of the samples presented parasites or ova. The presence of gram-negative bacteria was detected in 1.8% of the fecal samples. These results suggest that parasites and gram-negative bacteria may be transient in the gastrointestinal tract of healthy free-living psittacine birds. This situation is quite different from what is observed for birds in captivity, where stressful events and improper husbandry facilitate the dysbiosis and high parasite loads, with an imbalance in the host-parasite relationship.

Embora o Brasil tenha uma grande diversidade de aves, as informações sobre o estado sanitário de psitacídeos de vida livre são escassas. O objetivo deste trabalho foi determinar a prevalência de endoparasitas e a relação entre bactérias gram-negativas e gram-positivas nas fezes de Arara-azuis e Araras-vermelhas-grandes adultas em vida-livre. Amostras de fezes foram coletadas empregando-se lonas camufladas onde as aves se reuniam para forragear. Cinquenta e cinco esfregaços foram avaliados pela técnica de gram e 101 amostras de fezes foram analisadas pelos métodos de flutuação e centrífugo-flutuação.  Nenhuma amostra foi positiva para a presença de ovos ou formas adultas de parasitas. A presença de bactérias gram-negativas foi detectada em 1.8% das amostras fecais. Esses resultados sugerem que parasitas e bactérias gram-negativas podem ser apenas transientes no trato gastrointestinal de psitacídeos saudáveis de vida livre. Essa situação é bastante diversa do que ocorre em cativeiro, onde situações de estresse e manejo inadequado facilitam a disbiose e o aumento das cargas parasitárias, com um desequilíbrio na relação hospedeiro-parasita.

[Nesting habitat characterization for Amazona oratrix (Psittaciformes: Psittacidae) in the Central Pacific, Mexico]. / Hábitat de anidación de Amazona oratrix (Psittaciformes: Psittacidae) en el Pacífico Central, México.

The nesting requirements of the Yellow-headed Parrot (Amazona oratrix) are poorly understood, despite their broad historical distribution, high demand for pet trade and current endangered status. Information concerning their nesting requirements is required in order to design specific restoration and conser- vation actions. To assess this, we studied their nesting ecology in the Central Pacific, Michoacan, Mexico during a ten year period. The analyzed variables ranged from local scale nest site characteristics such as nesting tree species, dimensions, geographic positions, diet and nesting forest patches structure, to large scale features such as vegetation use and climatic variables associated to the nesting tree distributions by an ecological niche model using Maxent. We also evaluated the parrot tolerance to land management regimes, and compared the Pacific nest trees with 18 nest trees recorded in an intensively managed private ranch in Tamaulipas, Gulf of Mexico. Parrots nested in tall trees with canopy level cavities in 92 nest-trees recorded from 11 tree species. The 72.8% of nesting occurred in trees of Astronium graveolens, and Enterolobium cyclocarpum which qualified as key- stone trees. The forests where the parrots nested, presented a maximum of 54 tree species, 50% of which were identified as food source; besides, these areas also had a high abundance of trees used as food supply. The lowest number of tree species and trees to forage occurred in an active cattle ranch, whereas the highest species rich- ness was observed in areas with natural recovery. The nesting cavity entrance height from above ground of the Pacific nesting trees resulted higher than those found in the Gulf of Mexico. We hypothesize that the differences may be attributed to Parrot behavioral differences adapting to differential poaching pressure and cavity avail- ability. Nesting trees were found in six vegetation types; however the parrots preferred conserved and riparian semi-deciduous forest for nesting, with fewer nests in deciduous forest, while nesting in transformed agricultural fields was avoided. The main climatic variables associated with the potential distribution of nests were: mean temperature of wettest quarter, mean diurnal temperature range, and precipitation of wettest month. Suitable cli- matic conditions for the potential presence of nesting trees were present in 61% of the region; however, most of the area consisted of tropical deciduous forests (55.8%), while semi-deciduous tropical forests covered only 17% of the region. These results indicated the importance to conserve semi-deciduous forests as breeding habitats for the Yellow-headed Parrot, and revealed the urgent need to implement conservation and restoration actions. These should include a total ban of land use change in tropical semi-deciduous forest areas, and for selective logging of all keystone tree species; besides, we recommend the establishment of wildlife sanctuaries in important nesting areas, and a series of tropical forest restoration programs in the Central Pacific coast.

Crop damage of Eriotheca gracilipes (Bombacaceae) by the Blue-Fronted Amazon (Amazona aestiva, Psittacidae), in the Brazilian Cerrado / Predação pré-dispersão de sementes de Eriotheca gracilipes (Bombacaceae), pelo Papagaio verdadeiro (Amazona aestiva, Psittacidae) no Cerrado brasileiro

Seed predation has major effects on the reproductive success of individuals, spatial patterns of populations, genetic variability, interspecific interactions and ultimately in the diversity of tree communities. At a Brazilian savanna, I evaluated the proportional crop loss of Eriotheca gracilipes due the Blue-Fronted Amazon (Amazona aestiva) during a fruiting period. Also, I analyzed the relationship between proportional crop loss to Amazons and both fruit crop size and the distance from the nearest damaged conspecific. Trees produced from 1 to 109 fruits, so that Amazons foraged more often on trees bearing larger fruit crop size, while seldom visited less productive trees. Moreover, the relationship between fruit crop sizes and the number of depredated fruits was significant. However, when only damaged trees were assessed, I found a negative and significant relation between fruit crop size and proportional crop loss to Blue-Fronted Amazons. Taking into account this as a measure more directly related to the probability of seed survival, a negative density dependent effect emerged. Also, Amazons similarly damaged the fruit crops of either close or distant neighboring damaged trees. Hence, in spite of Blue-Fronted Amazons searched for E. gracilipes bearing large fruit crops, they were swamped due to the presence of more fruits than they could eat. Moderate seed predation by Blue-Fronted Amazons either at trees with large fruit crops or in areas where fruiting trees were aggregated implies in an enhanced probability of E. gracilipes seed survival and consequent regeneration success.(AU)

Avaliei durante um episódio de frutificação, no cerrado brasileiro, o impacto da predação de sementes, pelo Papagaio verdadeiro (Amazona aestiva), sobre as cargas de frutos da paineira do cerrado (Eriotheca gracilipes). Também, analisei as relações entre a taxa predação e tanto o tamanho das cargas de frutos, quanto à distância da E. gracilipes mais próxima predada. As ávores produziram de 1 a 109 frutos, sendo que os papagaios forragearam frequentemente em árvores com grandes cargas, enquanto raramente visitaram árvores com poucos frutos. Além disso, a relação entre tamanho da carga e número de frutos predados foi significativa. No entanto, a relação entre quantidade e proporção de frutos predados pelos Papagaios verdadeiros foi negativa e significativa. Considerando que a proporção de frutos predados é uma medida estreitamente relacionada às chances de sobrevivência das sementes, a predação pelos papagaios foi negativamente dependente da densidade. Apesar dos papagaios visitarem árvores com grandes cargas de frutos, foram saciados devido a presença de cargas superiores ao potencial de predação. Além disso, os papagaios danificaram igualmente as cargas de frutos tanto de árvores próximas quanto distantes entre si. O reduzido impacto pelos papagaios tanto sobre as grandes cargas de frutos quanto em áreas adensadas de E. gracilipes, implica em elevadas probabilidades de sobrevivência das sementes e respectiva chance de regeneração de E. gracilipes.(AU)

Crop damage of Eriotheca gracilipes (Bombacaceae) by the Blue-Fronted Amazon (Amazona aestiva, Psittacidae), in the Brazilian Cerrado / Predação pré-dispersão de sementes de Eriotheca gracilipes (Bombacaceae), pelo Papagaio verdadeiro (Amazona aestiva, Psittacidae) no Cerrado brasileiro

Seed predation has major effects on the reproductive success of individuals, spatial patterns of populations, genetic variability, interspecific interactions and ultimately in the diversity of tree communities. At a Brazilian savanna, I evaluated the proportional crop loss of Eriotheca gracilipes due the Blue-Fronted Amazon (Amazona aestiva) during a fruiting period. Also, I analyzed the relationship between proportional crop loss to Amazons and both fruit crop size and the distance from the nearest damaged conspecific. Trees produced from 1 to 109 fruits, so that Amazons foraged more often on trees bearing larger fruit crop size, while seldom visited less productive trees. Moreover, the relationship between fruit crop sizes and the number of depredated fruits was significant. However, when only damaged trees were assessed, I found a negative and significant relation between fruit crop size and proportional crop loss to Blue-Fronted Amazons. Taking into account this as a measure more directly related to the probability of seed survival, a negative density dependent effect emerged. Also, Amazons similarly damaged the fruit crops of either close or distant neighboring damaged trees. Hence, in spite of Blue-Fronted Amazons searched for E. gracilipes bearing large fruit crops, they were swamped due to the presence of more fruits than they could eat. Moderate seed predation by Blue-Fronted Amazons either at trees with large fruit crops or in areas where fruiting trees were aggregated implies in an enhanced probability of E. gracilipes seed survival and consequent regeneration success.

Avaliei durante um episódio de frutificação, no cerrado brasileiro, o impacto da predação de sementes, pelo Papagaio verdadeiro (Amazona aestiva), sobre as cargas de frutos da paineira do cerrado (Eriotheca gracilipes). Também, analisei as relações entre a taxa predação e tanto o tamanho das cargas de frutos, quanto à distância da E. gracilipes mais próxima predada. As ávores produziram de 1 a 109 frutos, sendo que os papagaios forragearam frequentemente em árvores com grandes cargas, enquanto raramente visitaram árvores com poucos frutos. Além disso, a relação entre tamanho da carga e número de frutos predados foi significativa. No entanto, a relação entre quantidade e proporção de frutos predados pelos Papagaios verdadeiros foi negativa e significativa. Considerando que a proporção de frutos predados é uma medida estreitamente relacionada às chances de sobrevivência das sementes, a predação pelos papagaios foi negativamente dependente da densidade. Apesar dos papagaios visitarem árvores com grandes cargas de frutos, foram saciados devido a presença de cargas superiores ao potencial de predação. Além disso, os papagaios danificaram igualmente as cargas de frutos tanto de árvores próximas quanto distantes entre si. O reduzido impacto pelos papagaios tanto sobre as grandes cargas de frutos quanto em áreas adensadas de E. gracilipes, implica em elevadas probabilidades de sobrevivência das sementes e respectiva chance de regeneração de E. gracilipes.

Hábitat de anidación de Amazona oratrix (Psittaciformes: Psittacidae) en el Pacífico Central, México / Nesting habitat characterization for Amazona oratrix (Psittaciformes: Psittacidae) in the Central Pacific, Mexico

The nesting requirements of the Yellow-headed Parrot (Amazona oratrix) are poorly understood, despite their broad historical distribution, high demand for pet trade and current endangered status. Information concerning their nesting requirements is required in order to design specific restoration and conservation actions. To assess this, we studied their nesting ecology in the Central Pacific, Michoacan, Mexico during a ten year period. The analyzed variables ranged from local scale nest site characteristics such as nesting tree species, dimensions, geographic positions, diet and nesting forest patches structure, to large scale features such as vegetation use and climatic variables associated to the nesting tree distributions by an ecological niche model using Maxent. We also evaluated the parrot tolerance to land management regimes, and compared the Pacific nest trees with 18 nest trees recorded in an intensively managed private ranch in Tamaulipas, Gulf of Mexico. Parrots nested in tall trees with canopy level cavities in 92 nest-trees recorded from 11 tree species. The 72.8% of nesting occurred in trees of Astronium graveolens, and Enterolobium cyclocarpum which qualified as keystone trees. The forests where the parrots nested, presented a maximum of 54 tree species, 50% of which were identified as food source; besides, these areas also had a high abundance of trees used as food supply. The lowest number of tree species and trees to forage occurred in an active cattle ranch, whereas the highest species richness was observed in areas with natural recovery. The nesting cavity entrance height from above ground of the Pacific nesting trees resulted higher than those found in the Gulf of Mexico. We hypothesize that the differences may be attributed to Parrot behavioral differences adapting to differential poaching pressure and cavity availability. Nesting trees were found in six vegetation types; however the parrots preferred conserved and riparian semi-deciduous forest for nesting, with fewer nests in deciduous forest, while nesting in transformed agricultural fields was avoided. The main climatic variables associated with the potential distribution of nests were: mean temperature of wettest quarter, mean diurnal temperature range, and precipitation of wettest month. Suitable climatic conditions for the potential presence of nesting trees were present in 61% of the region; however, most of the area consisted of tropical deciduous forests (55.8%), while semi-deciduous tropical forests covered only 17% of the region. These results indicated the importance to conserve semi-deciduous forests as breeding habitats for the Yellow-headed Parrot, and revealed the urgent need to implement conservation and restoration actions. These should include a total ban of land use change in tropical semi-deciduous forest areas, and for selective logging of all keystone tree species; besides, we recommend the establishment of wildlife sanctuaries in important nesting areas, and a series of tropical forest restoration programs in the Central Pacific coast. Rev. Biol. Trop. 62 (3): 1053-1072. Epub 2014 September 01.

Los requerimientos de anidación del loro cabeza-amarilla (Amazona oratrix) son poco conocidos a pesar de su amplia distribución histórica, la alta demanda como mascota y estar en peligro de extinción. Esta información es necesaria para diseñar acciones específicas de restauración y conservación. Durante diez años estudiamos la ecología de anidación del loro en Michoacán, México. Las variables estudiadas incluyeron en escala local las especies de árboles-nido, sus dimensiones y posición geográfica, dieta y estructura de parches de bosque; y a mayor escala el uso de la vegetación y las variables climáticas asociadas a la distribución de árboles-nido, obtenidas con un modelo de nicho ecológico utilizando Maxent. También evaluamos la tolerancia del loro al manejo de la tierra, y comparamos los árboles-nido encontrados, con 18 árboles-nido de Tamaulipas (Golfo de México), estudiados en los ochentas en un rancho privado intensamente manejado. Los loros anidaron en árboles altos en cavidades del dosel, registramos 92 árboles-nido en 11 especies de árboles, pero el 72.8% se presentó en Astronium graveolens y Enterolobium cyclocarpum que califican como árboles clave. Los bosques de anidación presentan 54 especies de árboles como máximo, y 50% sirven como alimento, también las zonas presentan alta abundancia de árboles dieta. La menor riqueza de árboles y de árboles dieta se presentó en un rancho ganadero, mientras que las áreas en regeneración natural presentaron mayor riqueza. La altura de la entrada de la cavidad en los árboles-nido del Pacífico fue mayor que en los del Golfo de México. Hipotetizamos que las diferencias se deben al comportamiento del loro, adaptado a la presión diferencial de saqueo de nidos y disponibilidad de cavidades. Los árboles-nido se encontraron en seis tipos de vegetación, pero el loro prefirió el bosque tropical subcaducifolio conservado, y ribereño, anidó menos en bosque tropical caducifolio y nunca en campos agrícolas transformados, difiriendo de lo esperado por la disponibilidad de hábitat. Las principales variables climáticas asociadas con la distribución potencial de nidos son: temperatura promedio del trimestre más lluvioso, oscilación diurna de la temperatura, y precipitación del mes más lluvioso. Las áreas de presencia potencial de árboles-nido representan el 61% de la región, sin embargo la mayor parte es bosque tropical caducifolio (55.8%), y el bosque tropical subcaducifolio sólo ocupa 17%. Los resultados indican la importancia del bosque tropical subcaducifolio conservado como hábitat de reproducción del loro cabeza-amarilla, y muestran la urgente necesidad de implementar acciones de conservación y restauración. Las acciones deben incluir la prohibición del cambio de uso del suelo del bosque tropical subcaducifolio, y de la tala selectiva de especies clave, la creación de santuarios de vida silvestre en las áreas de anidación y programas de restauración de los bosques tropicales de la costa del Pacífico central.

Plant food resources exploited by Blue-and-Yellow Macaws (Ara ararauna, Linnaeus 1758) at an urban area in Central Brazil.

In this study, we described the food plants available to Blue-and-Yellow Macaws (Ara ararauna), its feeding habits and the relationship between these parameters with feeding niche breadth. We established four transects, each one 12 km long, to sample fruiting plants and the feeding habits of this macaw (monthly 40 h, of observations), at the urban areas of Três Lagoas (Mato Grosso do Sul, Brazil). During all studied months, macaws foraged for palm fruits, mainly Syagrus oleracea and Acrocomia aculeata fruit pulp, both available all year, as well as Caryocar brasiliense and Anacardium occidentale seeds, in the wet season. The year-round feeding activity of macaws suggests Três Lagoas city as an adequate feeding area. The permanent availability of plant food resources, potentially, resulted from the diverse fruiting patterns of exotic and, mainly, native plant species, which provided a variety of suitable fruit patches.

Reproductive parameters in the critically endangered Blue-throated Macaw: limits to the recovery of a parrot under intensive management.

Rediscovered in the wild twenty years ago, the breeding biology of wild Blue-throated Macaws remains largely unexplored, yet is essential to its effective conservation and recovery. Here, we analyse reproductive parameters in an intensively managed wild population of Blue-throated Macaws, providing the first data on the breeding biology of this critically endangered species. During the six-year study period, 2007-2012, the number of active breeding pairs either remained constant or decreased, depending on the site, and no new breeding pairs were discovered despite extensive searching. We documented nesting attempts in natural cavities in dead palms or live hardwoods, and artificial nest boxes. Egg-laying was concentrated during the end of dry season and the beginning of the wet season, August through December. Hatching failure was the greatest cause of egg losses. Half of the breeding attempts of Blue-throated Macaws produced at least one fledging, on average two, after a 85 days nestling period. An average of 4.3 nestlings per year fledged from all known wild nests combined. Each pair lost roughly 65% of its initial reproductive investment at each nesting attempt. In most successful nesting attempts of individualized pairs, a new nesting attempt was not detected the following year. All monitored breeding pairs showed high nest site fidelity, reusing hardwood-tree cavities and nest boxes. Our findings will aid conservation efforts by refining current actions and prompting new approaches towards the conservation and recovery of the Blue-throated Macaw.