Retrospective analysis of factors associated with umbilical diseases in foals.

In neonatal foals, umbilical remnants can be affected by infectious and non-infectious diseases. This study aimed to retrospectively evaluate historical, management and clinical factors that may be related to the occurrence of umbilical remnant diseases. Clinical reports of foals born or hospitalized within 24 h of life during the 2017-2021 foaling seasons were reviewed. Forty/183 foals (21.9 %) developed umbilical remnant diseases (URD group), while 143/183 foals (78.1 %) had normal umbilical remnants (NUR group). In the URD group, 24/40 (60 %) had a patent urachus, 16/40 (40 %) omphalo-arteritis, 4/40 (10 %) omphalo-phlebitis, 10/40 (25 %) urachitis, 9/40 (22.5 %) abscess, 3/40 (7.5 %) periumbilical hematoma and 12/40 (30 %) more than one condition. URD frequency was higher in foals hospitalized after birth than in those born at the hospital (17/46 vs 23/137; P = 0.0068), lower in those that had access to the paddock before three days of life (p = 0.0426) and higher in recumbent foals (P = 0.0001). URD occurred more frequently after dystocia (P = 0.0068), prolonged stage II parturition (19±20.51 min vs 13±6.41 in NUR group; P = 0.0279), traction at parturition (P = 0.0005), and in foals with lower APGAR scores (8±1.72 vs 9±0.86 in NUR; P = 0.0063). Sepsis (P = 0.0245), neonatal encephalopathy (P = 0.0014), meconium retention (P = 0.0241) and congenital flexural limb deformities (P = 0.0049) were the most common associated diseases. Umbilical cord (UC) coiling, abnormal UC rupture, umbilical hemorrhage and increased umbilical stump volume occurred more frequently in URD than in NUR group (P = 0.0329, P = 0.0191, P = 0.0007 and P < 0.00001, respectively). Recognition of the identified predisposing historical, management and clinical factors should prompt careful umbilical remnant monitoring in neonatal foals.

Analysis of histone H3K4me3 modifications in bovine placenta derived from different calf-production methods.

In ruminants, the overgrowth of offspring produced by in vitro fertilization (IVF) is a common problem. Abnormal epigenetic modifications caused by environmental factors during the early embryonic period are suspected as an aetiology of overgrowth. In this study, we investigated the genome-wide histone H3K4me3 profiles of bovine placentae that play a pivotal role in foetal development and compared their characteristics between artificial insemination (AI)- and IVF-derived samples. Cotyledons were harvested from the placentae obtained at parturition of 5 AI- and 13 IVF-derived calves, and chromatin immunoprecipitation sequencing was performed for H3K4me3. We confirmed no significant maternal tissue contamination in the samples we used. The revealed H3K4me3 profiles reflected the general characteristics of the H3K4me3 modification, which is abundantly distributed in the promoter region of active genes. By extracting common modifications from multiple samples, the genes involved in placenta-specific biological processes could be enriched. Comparison with the H3K4me3 modifications of blastocyst samples was also effective for enriching the placenta-specific features. Principal component analysis suggested the presence of differential H3K4me3 modifications in AI- and IVF-derived samples. The genes contributing to the difference were related to the developmental biological processes. Imprinted genes such as BEGAIN, ZNF215 and DLX5 were among the extracted genes. Principal component and discriminant analyses using only male samples categorized the samples into three groups based on foetal weight and calf-production methods. To our knowledge, this is the first study to profile the genome-wide histone modifications of bovine foetal placentae and reveal their differential characteristics between different calf-production methods.

Evaluation of blood minerals and oxidative stress changing pattern in prepartum and postpartum Achai and Holstein Friesian dairy cows.

This research focused on evaluating blood mineral levels and malondialdehyde (MDA) as an indicator of oxidative stress before and after giving birth in Achai and Holstein Friesian (HF) dairy cows. Blood samples were obtained from a total of 50 cows representing both breeds on the third, second, and first week prior to calving, as well as on the day of calving (day 0). Subsequently, samples were collected on the 1-3 weeks postpartum to allow a comprehensive evaluation of blood parameters throughout the peripartum period. Results showed a significant (p < .01) decrease in serum zinc (Zn) levels on the day of parturition in both breeds. Additionally, HF cows exhibited higher (p < .01) Zn levels on week 2 before giving birth. Interestingly, blood selenium (Se) concentration increased (p < .01) in HF cows on weeks 2 and 3 after calving. In contrast, Achai cows showed a rise (p < .01) in blood Se on week 3 of parturition. Blood copper (Cu) levels were higher (p < .01) on weeks 2 and 3 after parturition in HF cows and on third week before parturition compared to Achai cows, where serum Cu remained high on week 1 of parturition. The findings indicated that blood magnesium (Mg) was higher (p < .01) on third week before parturition in Achai cows and on weeks 2 and 3 after parturition. Serum calcium (Ca) was higher (p < .01) in both HF and Achai cows on weeks 2 and 3 after parturition, and lower (p < .01) on third week before giving birth in both breeds. The mean blood MDA levels in Achai cows were lower (p < .01) on weeks 1 to 3 postpartum or 3 weeks before giving birth. In HF cows, serum MDA increased (p < .01) just before 2 weeks of parturition and remained elevated until 3 weeks of parturition. Thus, both breeds exhibited a similar pattern of mineral fluctuations; yet, Achai cows demonstrated greater resilience to oxidative stress compared to HF cows during the transition period.

[Morbidity and mortality of mare and foal following dystocia - a literature review]. / Morbidität und Mortalität von Stute und Fohlen nach Dystokie ­ eine Literaturübersicht.

Dystocia represents a life-threatening condition for mare and foal. Morbidity and mortality rates due to a difficult birth, as well as the influence on fertility of the mare were analyzed, based on a review of publications. This was aggravated by the fact that no standardized diagnostic code was used and that most publications do not clearly describe how extensively the examinations were performed beforehand. Retentio secundinarum is the most common complication caused by dystocia. Other complications are injuries to the soft birth canal and the colon. These occur more frequently after surgical obstetrics than following assisted vaginal deliveries. Performing a fetotomy increases the risk of injury to the birth canal. After a caesarean section, the risk for retained placenta increases significantly. In order to evaluate the possibility of medical progress over time, mortality rates of mare and foal were investigated and divided in surgical and conservative obstetrics within the period of 1970-1990 and 1991-2021. The average maternal mortality rate following caesarean section amounted to 18% in the time period between 1970 and 1990 and 14% between the years from 1991-2021. After fetotomy, the two determined mortality rates amounted to 29% and 10% for the time between 1970 and 1990. In the period 1991-2021, the rate varies between 4% and 44% with an average mortality rate of 14%. Following controlled vaginal delivery, the average mortality rate is 9%. Literary sources were however only available for the current time period and range between 6% and 29%. The morbidity and mortality rate of foals is very high. Following caesarean section on average 53% of foals are dead on delivery, with a range of 13-79% in case reports from the years 1991-2021. The number of dead foals in the context of conservative obstetrics is of a similar scale. Postnatal foal diseases are largely due to intrapartum hypoxia during dystocia and obstetric injury. In consequence of a difficult delivery, mares experience reduced fertility. In numerous cases this however may be compensated by pausing from further breeding in the same year. The number of cases evaluated in this context however remains too small to advocate any recommendations for breeding following incidents of dystocia.

[Diagnostic Imaging during pregnancy of the dog]. / Bildgebende Diagnostik im Rahmen der Trächtigkeit beim Hund.

Diagnostic imaging represents an important part during modern pregnancy management of the dog. Ultrasound has become the modality of choice for an early pregnancy diagnosis, enabling a verification as early as 17-20 days after LH surge. Furthermore an evaluation of fetal viability and development is possible as well as the depiction of malformations and an early assessment of fetal stress. Doppler imaging may also be of supporting value in this context . Using fetal maturity as well as measurements of fetal and extrafetal structures, a calculation of the gestational age and therefore the time of parturition is achievable. Radiographs of the caudal abdomen enable pregnancy diagnosis from the 43rd day after LH surge onward. In addition, an assessment of fetal size as well as litter size is possible. Both modalities allow for complementary statements concerning possible labor complications and possess supporting value concerning the decision of necessary obstetric intervention and further management in patients with dystocia.

Both spontaneous vaginal delivery and elective caesarean section influence neonatal redox status in dogs.

BACKGROUND: The influence of birth mode on redox balance is unknown in dogs. We aimed to compare antioxidant activity and oxidative stress of puppies born by elective c-section or vaginal delivery. METHODS: Twenty-one bitches were elected for spontaneous vaginal delivery (n = 10) and elective cesarean section (n = 11) groups, considering only the first and last puppies born. Neonatal oxygen saturation, lactatemia, superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and total antioxidant capacity; marker of oxidative stress (thiobarbituric acid reactive substances [TBARS]) and protein oxidation were analysed at birth, 1 h, 12 h, 24 h and 72 h. RESULTS: C-section newborns had lower oximetry at birth and 1 h. Lactatemia was higher in the last vaginal delivery puppies and higher at birth, and 1 h. Regardless of birth mode, last puppies had hyperlactatemia at birth. Vaginal delivery puppies had higher TBARS, while protein oxidation was higher in c-section, mostly in last puppies. C-section puppies had higher GPx activity, whereas GSH concentration decreased overtime. Surgically born puppies had lowering SOD between 12 h and 72 h. CONCLUSIONS: Vaginal delivery enhances lipid peroxidation, counteracted mostly by GPx. Conversely, c-section triggers protein oxidation, acutely scavenged by SOD and GSH, thus puppies are less exposed to the negative consequences of oxidative stress.

Climatic factors affecting gestational length in mares under subtropical climate.

The aim of this study was (i) to evaluate the relationship of climatic factors with gestational length (GL) and (ii) to evaluate the relationship of sire, foal gender and maternal factors with GL in mares. Retrospective data from 470 gestations of 202 respective mares were collected from a Criollo breeding farm in the southern hemisphere. GL was considered as the interval between ovulation and parturition. Climatic and environmental data (temperature, relative humidity, daily sunshine hours, precipitation, temperature humidity index - THI) were obtained daily and the mean values for foaling month and season were calculated. A multiple factor analysis of variance was performed to determine the relationship of the independent variables and interactions related to the mare, foal gender and stallion, and those related to the weather in each season of the year with GL as the dependent variable. The mean ± standard error (SE) of GL for the 470 gestations was 337 ± 0.4 days with a range of 311-363 days. Young-primiparous mares (340±0.9) had longer gestations (P < .001) than young-multiparous (336 ± 0.7) and mature-multiparous (334 ± 0.7) mares. Foal gender had no effect on GL. Gestational length was found to be affected by the stallion. Mares which experienced an autumn and winter gestational period with less daily sunshine hours had an increased GL (P < .05). In conclusion, environmental and climatic factors during pregnancy influenced the GL in mares, specifically, daily sunshine hours, precipitation and THI presented a negative correlation with GL. Young-primiparous Criollo mares presented longer gestational lengths than young or mature multiparous mares.

Risk factor analysis for beef calves requiring assisted vaginal delivery in Great Britain.

BACKGROUND: Dystocia has serious consequences for both mother and offspring. This study therefore aimed to identify risk factors for dystocia in vaginally delivered spring born beef calves in Great Britain (GB). METHODS: Information on calving assistance, calf sex, birthweight, breed, twinning, dam parity and body condition score (BCS) was collected from 1131 calves across 84 GB farms. Variables were modelled against calving assistance as a binary response variable. RESULTS: Twins (Odds Ratio [OR] = 5.45), Charolais calves (OR = 3.24), calves from primiparous dams (OR = 5.75) and male calves (OR = 1.75) were at significantly increased risk of requiring calving assistance across all models. Calves born to cows classed as thin (BCS < 2.5/5) were identified in the univariate analysis and in one of the multivariate models (OR = 1.92) as having an increased likelihood of dystocia. CONCLUSIONS: Most beef herds have limited scope to manage cows on the basis of fetal gender and number. However, calf breed, dam body condition and management of primiparous dams can be manipulated to reduce the risk of dystocia and improve supervision. Poor body condition is a novel risk factor for dystocia in beef cows and worthy of further investigation.

Malunion of an In Utero Metacarpal Fracture in a Standardbred Mare Presenting for Dystocia.

In utero fracture and malunion of long bones is a rare condition in horses. Most foals with in utero fractures are aborted, and the identification of a fetal in utero fracture in a mare with dystocia has not been reported. A 7-year-old multiparous Standardbred mare presented to a referral center for correction of dystocia. Assisted vaginal delivery and controlled vaginal delivery attempts were unsuccessful mainly because of contracted tendons impeding mutation. As the foal was alive, a cesarean section was elected. The foal was delivered but ultimately euthanized because of the congenital abnormalities. Computed tomography of the right forelimb of the foal along with gross examination and histologic evaluation of the right metacarpus revealed the malunion of a previous in utero fracture. While a few cases have been reported of in utero fracture, many of these were in abortuses and not in fetuses at term, making this case a new presentation and potential etiology for dystocia.

Remote monitoring system as a tool for calving management in Mediterranean Buffalo heifers (Bubalus bubalis).

Buffalo breeding is common in Southern Italy. Dystocia compromises dam's and newborn health and welfare. Difficult parturition could be solved through prompt calving assistance, even if the identification of the beginning of delivery is challenging. Herein, we aimed to evaluate a remote calving alarm system in 15 Mediterranean buffalo heifers. An intravaginal probe was placed close to the external cervical os once premonitory signs of delivery were observed. No vaginal discharge nor signs of discomfort were notified in the days following the insertion of the probe. Heifers calved from 48 to 72 hr after the alarm was activated. The system correctly warned the farm personnel at the beginning of stage II of parturition, except for 2 cases. In the former, the intravaginal probe was expelled but the poor carrier network coverage negatively affected phone's signal quality; in the latter, recurrent vaginal prolapse was responsible for non-retention of the probe. Overall median expulsive phase was 68 ± 8 min, while the expulsion of a female calf took 54 ± 22.0 min and 90 ± 34.0 min in males, with significant difference (p =.02). Deliveries were homogeneously distributed across a 24-hr interval. No retention of foetal membranes nor metritis was identified at postpartum clinical examination. The calving alarm system used in this work was well tolerated in buffalo heifers. The introduction of smart technology in buffalo farming could contribute to the overall farm net return by reducing calf losses, especially for calves born from sexed-sorted semen, and by increasing animal welfare through quick resolution of dystocia. Further studies will be necessary to evaluate the net return in buffalo farms which will implement a remote calving alarm system on a wider population.

Estimating the net return of a remote calving alarm system in a dairy farm.

The aim of the study was to evaluate the net return of the implementation of a remote calving monitoring system for obstetrical and neonatal assistance on the herd economy in a dairy farm model. A total of 680 parturitions over a 7-yr period were evaluated. Age at first calving was restricted from 23 to 27 mo for primiparous cows to be included. Among groups of cows that were ready to calve in a 15-d interval, primiparous and multiparous were randomly assigned to the experimental group and monitored through a calving alarm system, whereas the others accounted for controls. Final parturition groups were as follows: control primiparous (CPP, n = 218), control multiparous (CM, n = 345), monitored primiparous (MPP, n = 56), and monitored multiparous (MM, n = 61). Monitored groups received prompt calving assistance and first neonatal care, whereas the presence of farm personnel was discontinuous for controls. A biological model was built considering significant differences in calf loss, early culling, milk production, and days open between groups. Then, a partial budget model was used to estimate costs and net return on a simulated herd of 100 lactating cows. Incidence of calf death was greater in control groups (11.06% and 10.73% in CPP and CM, respectively) compared with monitored cows (0.00% and 1.69% in MPP and MM, respectively). Multiparous cows with calf loss had increased relative risk (relative risk = 3.487) for early culling compared with multiparous counterparts with no neonatal loss. Daily milk production in the first 2 mo was 3.79 kg greater in multiparous cows with no dead calf, compared with their counterparts. A significant difference in median days open was found in MPP and CPP (118 and 148 d, respectively). In the final economic model, different simulations were analyzed. They were created assuming different prices or hypothesizing calving monitoring only in primiparous animals. The model estimated different, but always positive, net return. In conclusion, implementing a calving alarm system led to a net return from €37 to 90 per cow per year (€1 = US$1.15 at the time of the study). However, the device alone is not sufficient: it must be supported by qualified calving monitoring and assistance. Optimized personnel presence in the calving area at the right time leads to prompt calving and neonatal calf assistance and colostrum feeding within the first hours of life, thus reducing calf death and days open, and increasing milk production.

Quantitative ultrasound elastography and biometry of the bitch uterus in the early puerperium after vaginal delivery and caesarean section.

Elastography is an actual imaging method used to qualitatively and quantitatively evaluate the elastic properties of tissues. The aim of this study was to evaluate and compare uterine tissue biometry and stiffness during post-partum period in brachycephalic bitches (n = 12) after c-section (GCS; n = 8) or normal delivery (GNB; n = 4). These animals were evaluated daily by abdominal ultrasound from the day of delivery until the 10th day post-partum; measuring uterine diameter, myometrial and endometrial thickness (mm) and shear wave velocity (SWV; m/s), by B-mode and ARFI (acoustic radiation force impulse) elastography, respectively. Uterine diameter was higher (p = .012) in animals submitted to c-section (15.26 ± 4.73 mm) than in normal birth (12.53 ± 2.64 mm) during the first 7 days post-partum. Uterine thickness gradually involuted in both groups (p < .0001), the myometrium during the first 9 days (p = .005) and the endometrium during the first 6 days (p = .003). The myometrial and endometrial SWVs were similar between types of delivery (p = .7846 and .8273) and presented a gradual increase (p = .411; .0043, respectively), during the first 10 days post-partum. It was concluded that bitches with normal delivery had smaller uterine thickness and faster puerperal involution than submitted to c-section, while uterine tissue stiffness was similar between delivery types and increased gradually during post-partum.

Comparison of Foaling Prediction Technologies in Periparturient Standardbred Mares.

The ability to predict impending foaling is invaluable, as attending foaling improves mare and foal outcome during parturition; therefore, a comparison of foaling prediction technologies is warranted. The purpose of this study was to compare technologies to predict foaling. Forty Standardbred mares were monitored daily for parturition. Prefoaling mammary secretion was collected daily and evaluated using a: calcium titration method (Ca titration), calcium and magnesium strip (Ca and Mg), handheld digital pH device (pH digital), pH strip, and refractometer (Brix). In addition, a birth monitoring system that was attached in the mare's vulva was studied and all positive alarms (foaling) and negative alarms (false alerts) were recorded. Analysis of variance, correlation, and logistic analysis were used to analyze the different technologies and their associations between parity and foal gender. Receiver operator characteristic analysis for cutoff value and subsequently sensitivity, specificity, positive, and negative predictive values for each technology were obtained. Maiden mares and mares carrying a colt had a longer gestational length. Multiparous and maiden mares had no difference in their prefoaling mammary secretion pH using the digital and pH strip technologies when predicting foaling; however, the calcium concentrations measured using the Ca titration technology were lower in multiparous than in maiden mares. The Ca titration technology was the single best technology to predict when the mare was not going to foal; and when the Ca titration and pH digital were used in combination, their prediction for foaling increased when compared with using each technology individually.

Clinical and pathological findings for rabbits with dystocia: 10 cases (1996-2016).

OBJECTIVE: To characterize clinical and pathological findings of rabbits evaluated at a veterinary teaching hospital because of dystocia. DESIGN: Retrospective case series. ANIMALS: 9 client-owned rabbits and 1 wild rabbit with signs of dystocia evaluated at a veterinary teaching hospital from 1996 through 2016. PROCEDURES: Medical records of rabbits were reviewed to collect data on signalment; medical history; physical examination, laboratory, diagnostic imaging, and procedural findings; treatment; final diagnosis; and outcome. Data were summarized. RESULTS: Dystocia in 7 rabbits was successfully managed through medical treatment, assisted vaginal delivery, or both (n = 6) or surgery alone (1); 3 rabbits were euthanized. Primiparous does, does ≤ 4 years old, and does of small breeds (< 2 kg [4.4 lb]) were most common. All client-owned rabbits had clinical signs of abnormal second-stage parturition, whereas the wild rabbit had only hemorrhagic vulvar discharge. Imaging was used to identify the number, size, and state of fetuses in most rabbits. Overall, 35 fetuses were accounted for, 25 of which were dead or later died. The cause of dystocia was determined for 8 rabbits and included fetal-maternal mismatch (n = 4), uterine inertia (2), fetal death or mummification (1), and stress-induced abortion (1). CONCLUSIONS AND CLINICAL RELEVANCE: Obstructive dystocia from fetal macrosomia with or without secondary uterine inertia was the most common cause of dystocia in the evaluated rabbits. Although medical management was successful for many rabbits with dystocia in this study, surgery could still be required in other affected rabbits, particularly when fetal-maternal mismatch is involved.

Influence of denaverine hydrochloride on calving ease in Holstein-Friesian heifers.

Calving is assumed to be an exhausting and painful event. A drug that eases the calving procedure and alleviates pain would help cows, especially those suffering from dystocia. In a randomized, controlled, and blinded trial, we measured the effect of denaverine hydrochloride on physical and physiological calving parameters. Eighty-three Holstein-Friesian heifers were included in the analysis. Pulling force was measured using a digital force gauge interposed between the calf and a mechanical calf puller. The concentration of cortisol was measured in serum before and after parturition. There was no effect of treatment group on calving modality (i.e., spontaneous vs. assisted calving), duration of calving, and cortisol concentration. The area under the curve of pulling force × time (n = 44), however, was significantly smaller in the treatment group compared with the placebo group. Also, duration of calving assistance was numerically shorter in the treatment group compared with the placebo group. The results provide evidence that calving ease can be influenced by denaverine hydrochloride during calving assistance.

Oxidative stress and acid-base balance during the transition period of neonatal Holstein calves submitted to different calving times and obstetric assistance.

During the neonatal period, constant adaptations to the extrauterine environment are required. These adaptations are made impossible or hindered when unexpected obstetrical events take place. This study aimed to identify clinical changes of vitality, metabolic and acid-base balance, and oxidative stress of newborn calves born to assisted dams that experienced different durations of calving. A prospective and observational study was conducted with 44 Holstein calves allocated to 3 groups according to the duration of calving: 2 h (n = 16), 2-4 h (n = 16), and >4 h (n = 12), and to 2 subgroups: spontaneous calving (n = 22) and assisted calving (n = 22). We observed that obstetric assistance was beneficial when calving lasted more than 4 h, because 100% of assisted calves had a high Apgar score (≥7) at 5 min, similar to that of spontaneously born calves after 2 h of calving. Rectal temperature of all calves remained within normal values, albeit with a reduction at 20 min of life. Calves born after a calving time >4 h had the highest rectal temperatures, especially those that required obstetrical assistance. There was a progressive increase in blood oxygenation with time, but calves in the >4 h group showed the highest partial pressure of carbon dioxide at birth, whereas calves in the 2 h and 2-4 h groups had lower values. A decrease in partial pressure of CO2 was observed at 120 min in the 2-4 h and >4 h groups. In the 2-4 h group, calves with obstetrical assistance had higher oxidative stress (measured as thiobarbituric acid reactive substances, TBARS) at 120 min of life compared with that at birth. In spontaneously born calves, TBARS increased only after 240 min. In the >4 h group, TBARS increased progressively with time, regardless of obstetrical subgroup. Calves born spontaneously within the 2 h group had higher TBARS values at birth than calves in the >4 h group with obstetrical assistance. Calves in the >4 h group showed higher levels of glutathione peroxidase (an antioxidant enzyme) at birth than those in the remaining groups. Glutathione peroxidase decreased at 120 min in the >4 h group and at 240 min for the 2 h and 2-4 h groups. In conclusion, obstetric assistance did not improve neonatal vitality and blood oxygenation when calving lasted less than 2 h. Calves born after 2 h of calving showed decreased vitality, hypercapnia, hypoxemia, and increased antioxidant status (glutathione peroxidase).

Birth delivery mode alters perinatal cell death in the mouse brain.

Labor and a vaginal delivery trigger changes in peripheral organs that prepare the mammalian fetus to survive ex utero. Surprisingly little attention has been given to whether birth also influences the brain, and to how alterations in birth mode affect neonatal brain development. These are important questions, given the high rates of cesarean section (C-section) delivery worldwide, many of which are elective. We examined the effect of birth mode on neuronal cell death, a widespread developmental process that occurs primarily during the first postnatal week in mice. Timed-pregnant dams were randomly assigned to C-section deliveries that were yoked to vaginal births to carefully match gestation length and circadian time of parturition. Compared with rates of cell death just before birth, vaginally-born offspring had an abrupt, transient decrease in cell death in many brain regions, suggesting that a vaginal delivery is neuroprotective. In contrast, cell death was either unchanged or increased in C-section-born mice. Effects of delivery mode on cell death were greatest for the paraventricular nucleus of the hypothalamus (PVN), which is central to the stress response and brain-immune interactions. The greater cell death in the PVN of C-section-delivered newborns was associated with a reduction in the number of PVN neurons expressing vasopressin at weaning. C-section-delivered mice also showed altered vocalizations in a maternal separation test and greater body mass at weaning. Our results suggest that vaginal birth acutely impacts brain development, and that alterations in birth mode may have lasting consequences.

The degree of visual cover and location of birth fluids affect dairy cows' choice of calving site.

Under natural conditions, cows seek isolation and visual cover when calving becomes imminent, but the degree of visual cover necessary to provide an attractive calving site is not known. When calving indoors, preparturient cows are attracted to other cows' birth fluids, and this may influence their isolation seeking. Therefore, we aimed to investigate the effect of degree of visual cover of secluded areas and the location of birth fluids on dairy cows' calving site selection. One hundred twenty-four Danish Holstein cows were moved in groups of 8 to 12 to a group pen with access to 6 areas secluded by a barrier of either 3 m (wide barrier) or 1.5 m (narrow barrier). Sixty cows calved within 14 d of having been moved to the group pen and were included in the study. From 3 h before partition and until the cow and calf were removed from the pen within 3 h after parturition, the location and posture of cows were recorded, along with the location of where their birth fluids were discharged. In addition, location and posture of calves were recorded during and up to 3 h after calving. All data were collected from continuous video recordings. Continuous data for location and behavior were analyzed by mixed models, binary data were analyzed by chi-squared analysis, and location of calving relative to where the cow's own or alien birth fluids were discharged was analyzed using logistic regression models. Ten percent of the cows calved in a secluded area, with no effect of barrier width. However, before calving, cows with access to secluded areas with a wide barrier spent more time in a secluded area (22 vs. 7 min/3 h), and more cows were observed lying there (58 vs. 28%) than cows with access to secluded areas with a narrow barrier. Secluded areas with a wide barrier were used by more cows and calves during the first hour after calving (36 vs. 10%). Among the cows that entered a secluded area after calving, the latency to enter correlated with their calf's latency to enter, suggesting that the 2 sought seclusion together. Seventy-nine percent of the cows calved in proximity (within a 1.25-m radius) to their own or an alien cow's birth fluids. There was no effect of barrier width on the probability of calving in proximity to their own or alien birth fluids. However, the probability of calving in proximity to their own or alien birth fluids was higher than the probability of not doing so. The greater use of secluded areas with a 3-m-wide barrier before and after parturition suggests that these are preferred over areas with a 1.5-m-wide barrier. Only a few cows calved in a secluded area, and more research is needed to explore the environmental and social factors affecting parturient cows' use of designated calving areas. The majority of cows calved close to where their own or alien birth fluids were discharged, suggesting that cows are attracted to these before calving. This may have affected the use of secluded areas as calving sites.

Birth delivery method affects expression of immune genes in lung and jejunum tissue of neonatal beef calves.

BACKGROUND: Caesarean section is a routine veterinary obstetrical procedure employed to alleviate dystocia in cattle. However, CS, particularly before the onset of labour, is known to negatively affect neonatal respiration and metabolic adaptation in humans, though there is little published information for cattle. The aim of this study was to investigate the effect of elective caesarean section (ECS) or normal trans-vaginal (TV) delivery, on lung and jejunal gene expression profiles of neonatal calves. RESULTS: Paternal half-sib Angus calves (gestation length 278 + 1.8 d) were delivered either transvaginally (TV; n = 8) or by elective caesarean section (ECS; n = 9) and immediately euthanized. Lung and jejunum epithelial tissue was isolated and snap frozen. Total RNA was extracted using Trizol reagent and reverse transcribed to generate cDNA. For lung tissue, primers were designed to target genes involved in immunity, surfactant production, cellular detoxification, membrane transport and mucin production. Primers for jejunum tissue were chosen to target mucin production, immunoglobulin uptake, cortisol reaction and membrane trafficking. Quantitative real-time PCR reactions were performed and data were statistically analysed using mixed models ANOVA. In lung tissue the expression of five genes were affected (p < 0.05) by delivery method. Four of these genes were present at lower (LAP, CYP1A1, SCN11α and SCN11ß) and one (MUC5AC) at higher abundance in ECS compared with TV calves. In jejunal tissue, expression of TNFα, Il-1ß and 1 l-6 was higher in ECS compared with TV calves. CONCLUSIONS: This novel study shows that ECS delivery affects the expression of key genes involved in the efficiency of the pulmonary liquid to air transition at birth, and may lead to an increased inflammatory response in jejunal tissue, which could compromise colostral immunoglobulin absorption. These findings are important to our understanding of the viability and management of neonatal calves born through ECS.