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1.
J Neurosci ; 37(34): 8166-8179, 2017 08 23.
Artigo em Inglês | MEDLINE | ID: mdl-28733354

RESUMO

Temporal lobe epilepsy (TLE) is the most frequent form of focal epilepsies and is generally associated with malfunctioning of the hippocampal formation. Recently, a preferential loss of parvalbumin (PV) neurons has been observed in the subiculum of TLE patients and in animal models of TLE. To demonstrate a possible causative role of defunct PV neurons in the generation of TLE, we permanently inhibited GABA release selectively from PV neurons of the ventral subiculum by injecting a viral vector expressing tetanus toxin light chain in male mice. Subsequently, mice were subjected to telemetric EEG recording and video monitoring. Eighty-eight percent of the mice presented clusters of spike-wave discharges (C-SWDs; 40.0 ± 9.07/month), and 64% showed spontaneous recurrent seizures (SRSs; 5.3 ± 0.83/month). Mice injected with a control vector presented with neither C-SWDs nor SRSs. No neurodegeneration was observed due to vector injection or SRS. Interestingly, mice that presented with only C-SWDs but no SRSs, developed SRSs upon injection of a subconvulsive dose of pentylenetetrazole after 6 weeks. The initial frequency of SRSs declined by ∼30% after 5 weeks. In contrast to permanent silencing of PV neurons, transient inhibition of GABA release from PV neurons through the designer receptor hM4Di selectively expressed in PV-containing neurons transiently reduced the seizure threshold of the mice but induced neither acute nor recurrent seizures. Our data demonstrate a critical role for perisomatic inhibition mediated by PV-containing interneurons, suggesting that their sustained silencing could be causally involved in the development of TLE.SIGNIFICANCE STATEMENT Development of temporal lobe epilepsy (TLE) generally takes years after an initial insult during which maladaptation of hippocampal circuitries takes place. In human TLE and in animal models of TLE, parvalbumin neurons are selectively lost in the subiculum, the major output area of the hippocampus. The present experiments demonstrate that specific and sustained inhibition of GABA release from parvalbumin-expressing interneurons (mostly basket cells) in sector CA1/subiculum is sufficient to induce hyperexcitability and spontaneous recurrent seizures in mice. As in patients with nonlesional TLE, these mice developed epilepsy without signs of neurodegeneration. The experiments highlight the importance of the potent inhibitory action mediated by parvalbumin cells in the hippocampus and identify a potential mechanism in the development of TLE.


Assuntos
Hipocampo/fisiopatologia , Interneurônios/fisiologia , Parvalbuminas/antagonistas & inibidores , Parvalbuminas/fisiologia , Convulsões/fisiopatologia , Animais , Eletroencefalografia/métodos , Hipocampo/efeitos dos fármacos , Potenciais Pós-Sinápticos Inibidores/efeitos dos fármacos , Potenciais Pós-Sinápticos Inibidores/fisiologia , Interneurônios/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Técnicas de Cultura de Órgãos , Pentilenotetrazol/toxicidade , Convulsões/induzido quimicamente
2.
Brain Res ; 1531: 37-47, 2013 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-23891794

RESUMO

It has recently been shown that expression of the rate-limiting GABA-synthesizing enzyme glutamic acid decarboxylase (GAD) is decreased in Brodmann area 9 (BA9) of the dorsolateral prefrontal cortex (DLPFC) in Parkinson's disease (PD) compared to control brains (Lanoue, A.C., Dumitriu, A., Myers, R.H., Soghomonian, JJ., 2010. Exp. Neurol. 206 (1), 207-217). A subpopulation of cortical GABAergic interneurons expresses the calcium-binding protein parvalbumin and plays a critical role in the control of pyramidal neuron excitability and the generation of cortical gamma frequency oscillations. In view of its key role in the physiology of the cerebral cortex, we sought to determine whether the expression of parvalbumin and the number of parvalbumin-expressing neurons are altered in BA9 of PD brains. First, isotopic in situ hybridization histochemistry was used to examine mRNA expression of parvalbumin on post-mortem brain sections. Second, immunohistochemistry and design-based stereology were used to determine the density of parvalbumin-positive interneurons in BA9. Quantification of mRNA labeling at the single cell level showed a significant decrease in parvalbumin expression in PD cases. In contrast, neuronal density of parvalbumin-positive neurons was not significantly different between PD and controls. Results confirm that the GABAergic system is altered in the DLPFC in PD and identify the contribution of parvalbumin-expressing neurons in these alterations. We speculate that these effects could contribute to altered cortical excitability and oscillatory activity previously documented in PD.


Assuntos
Regulação da Expressão Gênica , Neurônios/metabolismo , Doença de Parkinson/metabolismo , Parvalbuminas/antagonistas & inibidores , Córtex Pré-Frontal/metabolismo , RNA Mensageiro/antagonistas & inibidores , Adulto , Idoso , Idoso de 80 Anos ou mais , Contagem de Células , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neurônios/patologia , Doença de Parkinson/genética , Doença de Parkinson/patologia , Parvalbuminas/biossíntese , Parvalbuminas/genética , Córtex Pré-Frontal/patologia , RNA Mensageiro/biossíntese , RNA Mensageiro/genética
3.
Neuroscience ; 208: 58-68, 2012 Apr 19.
Artigo em Inglês | MEDLINE | ID: mdl-22640876

RESUMO

One of the primary lines of defense against oxidative stress is the selenoprotein family, a class of proteins that contain selenium in the form of the 21st amino acid, selenocysteine. Within this class of proteins, selenoprotein P (Sepp1) is unique, as it contains multiple selenocysteine residues and is postulated to act in selenium transport. Recent findings have demonstrated that neuronal selenoprotein synthesis is required for the development of parvalbumin (PV)-interneurons, a class of GABAergic neurons involved in the synchronization of neural activity. To investigate the potential influence of Sepp1 on PV-interneurons, we first mapped the distribution of the Sepp1 receptor, ApoER2, and parvalbumin in the mouse brain. Our results indicate that ApoER2 is highly expressed on PV-interneurons in multiple brain regions. Next, to determine whether PV-interneuron populations are affected by Sepp1 deletion, we performed stereology on several brain regions in which we observed ApoER2 expression on PV-interneurons, comparing wild-type and Sepp1(-/-) mice. We observed reduced numbers of PV-interneurons in the inferior colliculus of Sepp1(-/-) mice, which corresponded with a regional increase in oxidative stress. Finally, as impaired PV-interneuron function has been implicated in several neuropsychiatric conditions, we performed multiple behavioral tests on Sepp1(-/-) mice. Our behavioral results indicate that Sepp1(-/-) mice have impairments in contextual fear extinction, latent inhibition, and sensorimotor gating. In sum, these findings demonstrate the important supporting role of Sepp1 on ApoER2-expressing PV-interneurons.


Assuntos
Medo/fisiologia , Deleção de Genes , Interneurônios/patologia , Deficiências da Aprendizagem/metabolismo , Parvalbuminas/antagonistas & inibidores , Selenoproteína P/deficiência , Filtro Sensorial/genética , Animais , Feminino , Interneurônios/metabolismo , Deficiências da Aprendizagem/genética , Deficiências da Aprendizagem/patologia , Masculino , Camundongos , Camundongos Knockout , Estresse Oxidativo/genética , Parvalbuminas/fisiologia , Selenoproteína P/genética
4.
Brain Res ; 1437: 58-68, 2012 Feb 09.
Artigo em Inglês | MEDLINE | ID: mdl-22226504

RESUMO

The circling (cir) mouse strain, a murine model of deafness caused by a spontaneous mutation, exhibits characteristic behaviors of circling and hyperactivity. In an induced-noise paradigm, cir mice display a significant loss in their spatial orientation abilities, and this has been suggested to be due at least in part to changes in calcium homeostasis. Auditory information is transferred from the cochlear nucleus to the hippocampus, where it is processed to modulate motor and sensory activity. Such a pathway could be affected at the cellular level by alterations in neurotransmission, including alterations that involve Ca(2+). However, there have been no studies in a hearing deficit model examining the concomitant molecular alterations in the hippocampus. Thus, in the present study we used immunohistochemistry to compare the distribution of the calcium-binding proteins (CaBPs) calbindin D-28k, parvalbumin, and calretinin in the hippocampi of heterozygous (+/cir), homozygous (cir/cir), and wild-type (+/+) mice. The expression of the CaBPs in various hippocampal subfields appeared to be significantly lower in cir mice (+/- and -/-) than in +/+ mice. Such a decrease in CaBP expression in cir/cir mice would alter calcium homeostasis, which in turn could affect the connection of the tri-synaptic circuit of the hippocampus as well as the cortical region. A decrease in CaBPs and the probable resultant glutamate-mediated excitability could contribute to the functional changes that lead to the characteristic behavioral features of cir mice.


Assuntos
Hipocampo/metabolismo , Parvalbuminas/antagonistas & inibidores , Parvalbuminas/metabolismo , Proteína G de Ligação ao Cálcio S100/antagonistas & inibidores , Proteína G de Ligação ao Cálcio S100/metabolismo , Animais , Calbindina 2 , Calbindinas , Surdez/genética , Surdez/metabolismo , Regulação para Baixo/genética , Hipocampo/química , Hipercinese/genética , Hipercinese/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Mutantes Neurológicos , Parvalbuminas/genética , Proteína G de Ligação ao Cálcio S100/genética
5.
J Neurosci Res ; 89(7): 1043-51, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21488091

RESUMO

Taurine, 2-aminoethanesulfonic acid, is present at high concentrations in many invertebrate and vertebrate systems, and it has several biological functions. In addition, it has been related to a neuroprotective role against several diseases, such as epilepsy. It has been reported that taurine induces a decrease of calbindin-D28k, calretinin, and parvalbumin protein levels in the hippocampus 3 days after administration. In the present work we hypothesized that the decrease of these proteins could alter the action of kainic acid (KA) and make mice more susceptible to excitotoxicity. Therefore, we treated mice with taurine and after 3 days treated them with KA. The results showed that taurine pretreatment did not induce a major susceptibility to KA. Moreover, neurodegeneration was reduced in pretreated mice. However, astrogliosis was similar to that observed in mice treated only with KA. The immunohistochemistries for calbindin-D28k, calretinin, and parvalbumin showed that these proteins were reduced as a consequence of KA treatment and of taurine treatment. However, mice pretreated with taurine prior to KA administration presented the same reduction in these proteins as mice treated with only taurine or only KA.


Assuntos
Proteínas de Ligação ao Cálcio/antagonistas & inibidores , Resistência a Medicamentos/efeitos dos fármacos , Ácido Caínico/agonistas , Neurotoxinas/agonistas , Parvalbuminas/antagonistas & inibidores , Proteína G de Ligação ao Cálcio S100/antagonistas & inibidores , Taurina/toxicidade , Animais , Calbindina 1 , Calbindina 2 , Calbindinas , Proteínas de Ligação ao Cálcio/metabolismo , Modelos Animais de Doenças , Suscetibilidade a Doenças , Regulação para Baixo/efeitos dos fármacos , Regulação para Baixo/fisiologia , Resistência a Medicamentos/fisiologia , Epilepsia/induzido quimicamente , Epilepsia/tratamento farmacológico , Epilepsia/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos , Degeneração Neural/tratamento farmacológico , Degeneração Neural/metabolismo , Degeneração Neural/patologia , Parvalbuminas/metabolismo , Proteína G de Ligação ao Cálcio S100/metabolismo , Taurina/metabolismo
6.
Neurosci Lett ; 483(2): 132-6, 2010 Oct 11.
Artigo em Inglês | MEDLINE | ID: mdl-20691752

RESUMO

The spontaneous mutant circling mouse has an autosomal recessive pattern of inheritance and is an animal model for deafness, which is characterized by circling, head tossing, and hyperactivity. Since the main pathology in circling mice lies in the organ of Corti, most studies on deaf mice have focused on auditory brain stem nuclei. No studies regarding behavior-related CNS changes in circling mice have been reported. The major center of sensory input for modulation of motor activity is best-studied in the cerebellum. Considering the importance of calcium homeostasis in numerous processes, calcium-binding proteins (CaBPs), such as calbindin D-28k (CB), parvalbumin (PV), and calretinin (CR), may play crucial roles in preserving cerebellar coordinated motor function. Thus, the distribution of CB, PV, and CR was determined in the cerebellum using immunohistochemical methods to compare immunoreactivity (IR) of CaBPs between wild-type (+/+), heterozygous (+/cir), and homozygous (cir/cir) mice. The IR of CB and PV was predominantly observed in the Purkinje cell layer of all three genotypes. Compared with the +/+ genotype, the relative mean density of CB and PV IR in the Purkinje cell layer and CR IR in the granular layer was significantly decreased in the cir/cir genotype. Changes in calcium homeostasis in parallel fiber/Purkinje cell synapses could diminish cerebellar control of motor coordination. A number of deficiencies among the CaBPs lead to distinct alterations in brain physiology, which may affect normal behavior.


Assuntos
Córtex Cerebelar/metabolismo , Doenças Cerebelares/genética , Doenças Cerebelares/metabolismo , Parvalbuminas/metabolismo , Proteína G de Ligação ao Cálcio S100/metabolismo , Animais , Calbindina 2 , Calbindinas , Sinalização do Cálcio/genética , Córtex Cerebelar/patologia , Regulação para Baixo/genética , Genótipo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Mutantes Neurológicos , Parvalbuminas/antagonistas & inibidores , Parvalbuminas/genética , Células de Purkinje/metabolismo , Células de Purkinje/patologia , Proteína G de Ligação ao Cálcio S100/antagonistas & inibidores , Proteína G de Ligação ao Cálcio S100/genética , Vesículas Secretórias/genética , Vesículas Secretórias/metabolismo
7.
Dev Psychobiol ; 49(6): 606-18, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17680608

RESUMO

Age-dependent, MK801-induced, activated caspase-3 expression in the postnatal brain is generally not observed in neurons expressing calcium-binding proteins (CaBPs), suggesting that apoptosis and calcium buffering are inversely related. In regions such as the cingulate and retrosplenial cortex, injury peaks at postnatal Day 7 (P7) and rapidly diminishes thereafter, whereas expression of calbindin (CB) and calretinin (CR) was relatively low from P0 to P7 and steadily increased from P7 to P14. At ages thereafter, CB and CR expression either remained stable then declined or rapidly declined. Parvalbumin (PV) was generally low-absent prior to P7 but expression dramatically increased from P10 onwards, peaking at P21. These studies suggest calcium entry (through N-methyl-D-aspartate receptor (NMDARs)) and buffering (by CaBPs) are integral to normal CNS maturation. Because schizophrenia is associated with glutamate hypo-function, developmental injury, and aberrant CaBP expression, our data indicate that this postnatal brain injury model may offer important insights into the nature of this disorder.


Assuntos
Lesões Encefálicas/induzido quimicamente , Córtex Cerebral/efeitos dos fármacos , Córtex Cerebral/metabolismo , Maleato de Dizocilpina/efeitos adversos , Antagonistas de Aminoácidos Excitatórios/efeitos adversos , Giro do Cíngulo/efeitos dos fármacos , Giro do Cíngulo/metabolismo , Parvalbuminas/antagonistas & inibidores , Fatores Etários , Animais , Lesões Encefálicas/metabolismo , Calbindina 2 , Cálcio/metabolismo , Caspase 3/metabolismo , Contagem de Células , Ácido Glutâmico/metabolismo , Imuno-Histoquímica , Parvalbuminas/metabolismo , Ratos , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Receptores de N-Metil-D-Aspartato/metabolismo , Proteína G de Ligação ao Cálcio S100/metabolismo
8.
Res Commun Mol Pathol Pharmacol ; 103(3): 249-59, 1999 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10509736

RESUMO

The role of calcium binding proteins, calbindin D-28k (CaB) and parvalbumin (PV) in Purkinje cell survival was investigated using oligonucleotide antisense strategy. Purkinje cell enriched cultures were prepared from the cerebella of 0-1 day old Balb/c mouse pups. Purkinje cells were identified by size, asymmetric arbors, immunoreactivity to CaB and PV, uptake of gamma-aminobutyric acid (GABA) and failure to express glial fibrillary acidic protein. The cells at different days in vitro were treated with antisense or mismatched antisense phosphorothioate oligonucleotides for CaB and PV mRNA (complexed with lipofectin). Neuronal specific [3H]-GABA uptake was used as a measure of Purkinje cell survival. The cultures treated for 24 h with antisense oligos (CaB+PV) showed a significant decrease in [3H]-GABA uptake as compared with the cultures treated with lipofectin alone or with lipofectin + mismatched antisense oligos to CaB and PV mRNA. The results of the present study suggest that the expression of calcium buffering proteins CaB and PV may have a significant involvement in Purkinje cell viability.


Assuntos
Oligonucleotídeos Antissenso/farmacologia , Parvalbuminas/antagonistas & inibidores , Células de Purkinje/efeitos dos fármacos , Proteína G de Ligação ao Cálcio S100/antagonistas & inibidores , Animais , Animais Recém-Nascidos , Anticorpos Monoclonais , Calbindinas , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Fluoresceína-5-Isotiocianato , Imunofluorescência , Camundongos , Camundongos Endogâmicos BALB C , Neuritos/metabolismo , Parvalbuminas/fisiologia , Células de Purkinje/citologia , Células de Purkinje/metabolismo , Proteína G de Ligação ao Cálcio S100/fisiologia , Fatores de Tempo , Tubulina (Proteína)/metabolismo , Ácido gama-Aminobutírico/farmacocinética
9.
Scand J Immunol ; 14(2): 207-11, 1981 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-7313563

RESUMO

A peptide with the sequence of the second calcium-binding loop (EF loop) of cod Allergen M was synthesized by automatic solid-phase technique. The synthetic peptide corresponded to residues 88-103 of the known primary structure of Allergen M. The immunochemical reactivity of this loop, previously demonstrated for the overlapping enzymic fragments, was confirmed by using the synthetic preparation. The purified hexadecapeptide was shown to bind specifically to reaginic IgE from sera of cod-allergic individuals, in both in vivo and in vitro tests systems. It could also bind rabbit anti-Allergen M, as shown by rocket line immunoelectrophoresis and quantitative precipitation inhibition techniques. The findings emphasized that the immunological reactivity of the synthetic peptide (88-103) was compatible with a monovalent haptenic function: blocking and not eliciting allergic reactions.


Assuntos
Alérgenos , Cálcio/metabolismo , Proteínas de Peixes , Proteínas Musculares , Parvalbuminas , Peptídeos/imunologia , Sequência de Aminoácidos , Animais , Sítios de Ligação , Peixes , Humanos , Imunoeletroforese , Parvalbuminas/antagonistas & inibidores , Peptídeos/síntese química , Peptídeos/isolamento & purificação , Teste de Radioalergoadsorção , Testes Cutâneos
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