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1.
Viruses ; 14(2)2022 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-35215841

RESUMO

A new dsRNA virus from the oomycete Phytophthora pluvialis has been characterized and designated as Phytophthora pluvialis RNA virus 1 (PplRV1). The genome of the PplRV1 reference genome is 6742 bp that encodes two predicted open reading frames (ORFs). ORF1 and ORF2 overlap by a 47 nt "slippery" frameshift sequence. ORF1 encodes a putative protein of unknown function. ORF2 shows high similarity to the RNA-dependent RNA polymerase (RdRp) of other dsRNA viruses. Phylogenetic analysis of the putative PplRV1 RdRp and its most closely related viruses showed PplRV1 is distinct from other known viruses (below 33% amino acid similarity), which indicates this virus may belong to a new virus family. Analyses of the geographical distribution of PplRV1 in relation to two genetically distinct classes of its host revealed two corresponding genotypes of the PplRV1 (termed a and b), which share 92.3% nt identity. The reference genome for the second genotype is 6760 bp long and a prediction of its genetic organization shows three ORFs, with ORF2 being split into two ORFs, ORF2a and ORF2b, that is conserved in seven of eleven genotype b isolates. Additionally, a quick and simple diagnostic method using qPCR has been developed, which is suitable for large scale screens to identify PplRV1 in Phytophthora.


Assuntos
Vírus de RNA de Cadeia Dupla/genética , Phytophthora/virologia , Sequência de Aminoácidos , Vírus de RNA de Cadeia Dupla/classificação , Genoma Viral/genética , Genótipo , Nova Zelândia , Fases de Leitura Aberta , Filogenia , RNA Viral/genética , RNA Polimerase Dependente de RNA/genética , Especificidade da Espécie
2.
Viruses ; 13(10)2021 09 25.
Artigo em Inglês | MEDLINE | ID: mdl-34696361

RESUMO

To further classify the oomycete viruses that have been discovered in recent years, we investigated virus infection in the plant-parasitic oomycete Globisporangium ultimum in Japan. Double-stranded RNA detection, high-throughput sequencing, and RT-PCR revealed that the G. ultimum isolate UOP226 contained two viruses related to fusarivirus and totivirus, named Pythium ultimum RNA virus 1 (PuRV1) and Pythium ultimum RNA virus 2 (PuRV2), respectively. Phylogenetic analysis of the deduced amino acid sequence of the RNA-dependent RNA polymerase (RdRp) showed that fusari-like PuRV1 belonged to a different phylogenetic group than Plasmopara viticola lesion-associated fusari virus (PvlaFV) 1-3 from oomycete Plasmopara viticola. Codon usage bias of the PuRV1 RdRp gene was more similar to those of fungi than Globisporangium and Phytophthora, suggesting that the PuRV1 ancestor horizontally transmitted to G. ultimum ancestor from fungi. Phylogenetic analysis of the deduced amino acid sequence of the RdRp of toti-like PuRV2 showed a monophyletic group with the other toti-like oomycete viruses from Globisporangium, Phytophthora, and Pl. viticola. However, the nucleotide sequences of toti-like oomycete viruses were not so homologous, suggesting the possibility of convergent evolution of toti-like oomycete viruses.


Assuntos
Genoma Viral , Oomicetos/virologia , Plantas/microbiologia , RNA Viral/classificação , RNA Viral/genética , Sequência de Bases , Fungos , Sequenciamento de Nucleotídeos em Larga Escala , Japão , Fases de Leitura Aberta , Filogenia , Phytophthora/virologia , Vírus de RNA/genética , RNA de Cadeia Dupla , RNA Polimerase Dependente de RNA/genética , Análise de Sequência de DNA , Totiviridae/genética
3.
Viruses ; 13(4)2021 04 04.
Artigo em Inglês | MEDLINE | ID: mdl-33916635

RESUMO

Marine oomycetes have recently been shown to be concurrently infected by (-)ssRNA viruses of the order Bunyavirales. In this work, even higher virus variability was found in a single isolate of Phytophthora condilina, a recently described member of Phytophthora phylogenetic Clade 6a, which was isolated from brackish estuarine waters in southern Portugal. Using total and small RNA-seq the full RdRp of 13 different potential novel bunya-like viruses and two complete toti-like viruses were detected. All these viruses were successfully confirmed by reverse transcription polymerase chain reaction (RT-PCR) using total RNA as template, but complementarily one of the toti-like and five of the bunya-like viruses were confirmed when dsRNA was purified for RT-PCR. In our study, total RNA-seq was by far more efficient for de novo assembling of the virus sequencing but small RNA-seq showed higher read numbers for most viruses. Two main populations of small RNAs (21 nts and 25 nts-long) were identified, which were in accordance with other Phytophthora species. To the best of our knowledge, this is the first study using small RNA sequencing to identify viruses in Phytophthora spp.


Assuntos
Phytophthora/virologia , Vírus de RNA/classificação , Vírus de RNA/genética , Pequeno RNA não Traduzido/genética , RNA Viral/genética , Análise de Sequência de RNA , Viroses/virologia , Biologia Computacional , Genoma Viral , Fases de Leitura Aberta , Filogenia , Portugal , Vírus de RNA/isolamento & purificação , RNA-Seq , Análise de Sequência de DNA
4.
Arch Virol ; 165(7): 1679-1682, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32367229

RESUMO

This report describes the complete genome sequence of a double-stranded RNA (dsRNA) virus infecting the oomycetous plant pathogen Phytophthora cactorum. The virus genome consists of a single dsRNA segment of 5699 bp with two open reading frames predicted to overlap with each other and encoding a putative capsid protein of 705 aa and an RNA-dependent RNA polymerase of 779 aa. Sequence comparisons indicated that this virus, designated as "Phytophthora cactorum RNA virus 1" (PcRV1), shares the highest sequence similarity with the unclassified Pythium splendens RNA virus 1 (58% RdRp aa sequence identity). Phylogenetic analysis revealed that these two oomycete viruses group together with Giardia lamblia virus (GVL; family Totiviridae) and several unclassified toti-like viruses from arthropods, fish and fungi. This is the first report of a toti-like virus in a member of the genus Phytophthora and the first virus characterized in P. cactorum.


Assuntos
Genoma Viral , Phytophthora/virologia , Totiviridae/genética , Sequência de Bases , Fases de Leitura Aberta , Filogenia , Doenças das Plantas/microbiologia , RNA de Cadeia Dupla/genética , RNA Viral/genética , Totiviridae/classificação , Totiviridae/isolamento & purificação
5.
Adv Virus Res ; 86: 327-50, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23498912

RESUMO

Phytophthora sp. is a genus in the oomycetes, which are similar to filamentous fungi in morphology and habitat, but phylogenetically more closely related to brown algae and diatoms and fall in the kingdom Stramenopila. In the past few years, several viruses have been characterized in Phytophthora species, including four viruses from Phytophthora infestans, the late blight pathogen, and an endornavirus from an unnamed Phytophthora species from Douglas fir. Studies on Phytophthora viruses have revealed several interesting systems. Phytophthora infestans RNA virus 1 (PiRV-1) and PiRV-2 are likely the first members of two new virus families; studies on PiRV-3 support the establishment of a new virus genus that is not affiliated with established virus families; PiRV-4 is a member of Narnaviridae, most likely in the genus Narnavirus; and Phytophthora endornavirus 1 (PEV1) was the first nonplant endornavirus at the time of reporting. Viral capsids have not been found in any of the above-mentioned viruses. PiRV-1 demonstrated a unique genome organization that requires further examination, and PiRV-2 may have played a role in late blight resurgence in 1980s-1990s.


Assuntos
Phytophthora/virologia , Vírus de RNA/isolamento & purificação , Ordem dos Genes , Genes Virais , Genoma Viral , Vírus de RNA/classificação , Vírus de RNA/genética
6.
Virus Genes ; 40(1): 130-4, 2010 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-19915969

RESUMO

In this investigation, we show that four Phytophthora taxon douglasfir isolates from the USA, irrespective of their geographical location or host plant, and 20% of a representative cohort of Phytophthora ramorum isolates contain endornavirus dsRNAs. Three endornavirus-specific RT-PCR amplicons were generated by RT-PCR using dsRNA isolated from the four Phytophthora taxon douglasfir isolates and one representative Phytophthora ramorum isolate as template with oligonucleotide primers designed from the sequence of Phytophthora endornavirus 1. The amplified segments showed a very high degree of sequence similarity suggesting that the virus has gone through a population bottleneck during its emergence.


Assuntos
Phytophthora/virologia , Vírus de RNA/isolamento & purificação , Filogenia , Vírus de RNA/genética
7.
J Gen Virol ; 86(Pt 5): 1561-1570, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15831970

RESUMO

A new dsRNA was isolated from a Phytophthora isolate from Douglas fir. Sequence analysis showed the dsRNA to consist of 13 883 bp and to contain a single open reading frame with the potential to encode a polyprotein of 4548 aa. This polyprotein contained amino acid sequence motifs characteristic of virus RNA-dependent RNA polymerases (RdRps) in its C-terminal region and motifs characteristic of RNA helicases in its N-terminal region. These sequence motifs were related to corresponding motifs in plant viruses in the genus Endornavirus. In phylogenetic trees constructed from the RdRp and helicase motifs of a range of ssRNA and dsRNA viruses, the Phytophthora RdRp and helicase sequences clustered with those of the plant endornaviruses with good bootstrap support. The properties of the Phytophthora dsRNA are consistent with its being classified as the first non-plant member of the genus Endornavirus, for which we propose the name phytophthora endornavirus 1 (PEV1). A region between the RdRp and helicase domains of the PEV1 protein had significant amino acid sequence similarity to UDP glycosyltransferases (UGTs). Two sequence motifs were identified, one characteristic of all UGTs and the other characteristic of sterol UGTs. The PEV1 UGT would be the first for an RNA virus, although ecdysteroid UGT genes have been found in many baculoviruses. The PEV1 UGT was only distantly related to baculovirus ecdysteroid UGTs, which belong to a family distinct from the sterol UGTs.


Assuntos
Glicosiltransferases/genética , Phytophthora/genética , Phytophthora/virologia , Vírus de RNA/genética , RNA de Cadeia Dupla/genética , RNA de Cadeia Dupla/isolamento & purificação , Motivos de Aminoácidos , Sequência de Aminoácidos , Sequência Conservada , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Vírus de Plantas/classificação , Vírus de Plantas/genética , RNA Helicases/genética , Vírus de RNA/classificação , RNA Polimerase Dependente de RNA/genética , Análise de Sequência , Análise de Sequência de DNA , Homologia de Sequência
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