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1.
Sci Rep ; 10(1): 17574, 2020 10 16.
Artigo em Inglês | MEDLINE | ID: mdl-33067553

RESUMO

Grapevine (Vitis vinifera L.) is a crop of major economic importance. However, grapevine yield is guaranteed by the massive use of pesticides to counteract pathogen infections. Under temperate-humid climate conditions, downy mildew is a primary threat for viticulture. Downy mildew is caused by the biotrophic oomycete Plasmopara viticola Berl. & de Toni, which can attack grapevine green tissues. In lack of treatments and with favourable weather conditions, downy mildew can devastate up to 75% of grape cultivation in one season and weaken newly born shoots, causing serious economic losses. Nevertheless, the repeated and massive use of some fungicides can lead to environmental pollution, negative impact on non-targeted organisms, development of resistance, residual toxicity and can foster human health concerns. In this manuscript, we provide an innovative approach to obtain specific pathogen protection for plants. By using the yeast two-hybrid approach and the P. viticola cellulose synthase 2 (PvCesA2), as target enzyme, we screened a combinatorial 8 amino acid peptide library with the aim to identify interacting peptides, potentially able to inhibit PvCesa2. Here, we demonstrate that the NoPv1 peptide aptamer prevents P. viticola germ tube formation and grapevine leaf infection without affecting the growth of non-target organisms and without being toxic for human cells. Furthermore, NoPv1 is also able to counteract Phytophthora infestans growth, the causal agent of late blight in potato and tomato, possibly as a consequence of the high amino acid sequence similarity between P. viticola and P. infestans cellulose synthase enzymes.


Assuntos
Aptâmeros de Peptídeos/farmacologia , Glucosiltransferases/antagonistas & inibidores , Oomicetos/efeitos dos fármacos , Doenças das Plantas/terapia , Proteínas de Plantas/antagonistas & inibidores , Proteínas Citotóxicas Formadoras de Poros/farmacologia , Sequência de Aminoácidos , Celulose/biossíntese , Glucosiltransferases/química , Oomicetos/enzimologia , Oomicetos/ultraestrutura , Biblioteca de Peptídeos , Fotossíntese , Phytophthora infestans/efeitos dos fármacos , Phytophthora infestans/enzimologia , Phytophthora infestans/ultraestrutura , Doenças das Plantas/parasitologia , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/enzimologia , Proteínas de Plantas/química , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Solanum tuberosum , Técnicas do Sistema de Duplo-Híbrido , Vitis
2.
Sci Rep ; 6: 20204, 2016 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-26822318

RESUMO

A series of 2,5-disubstituted-1,3,4-thiadiazoles were synthesized using Lawesson's reagent by an efficient approach under microwave irradiation in good yields. Their structures were characterized by MS, IR, (1)H NMR, (13)C NMR, and elemental analysis. Their in vitro and in vivo fungicidal activities revealed that the title compounds exhibited considerable activity against five selected fungi, especially to Phytophthora infestans. In order to illustrate the mechanism of title compounds against P. infestans, scanning electron micrographs (SEM) and transmission electron micrographs (TEM) were applied. The morphological and ultrastructural studies demonstrated that compound I18 led to swelling of hyphae, thickening and proliferating multilayer cell walls, excessive septation and accumulation of dense bodies. The bioassay results indicated compound I18 might act on cell wall biosynthesis, and blocked the nutrition transportation and led to cells senescence and death. Meanwhile, compound I18 had broad fungicidal activity against other twenty different kinds of fungi. These results suggested that title compounds were eligible to be development candidates and compound I18 as a promising lead compound was worthy to be further discovery, especially against P. infestans.


Assuntos
Antifúngicos , Phytophthora infestans/crescimento & desenvolvimento , Tiadiazóis , Antifúngicos/síntese química , Antifúngicos/química , Antifúngicos/farmacologia , Phytophthora infestans/ultraestrutura , Tiadiazóis/síntese química , Tiadiazóis/química , Tiadiazóis/farmacologia
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