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1.
J Pediatr Hematol Oncol ; 43(4): e505-e507, 2021 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-32769571

RESUMO

Growing teratoma syndrome is a well-recognized condition associated with both intracranial and extracranial nongerminomatous germ cell tumors (NGGCTs), which mostly manifest as rapid growth of cystic and solid components during or within several months after treatment. Here, we report a patient with NGGCT who experienced slow growth of intracranial growing teratoma syndrome with intraventricular lipid accumulation over 10 years without any clinical symptoms. Considering the clinicopathologic heterogeneity of this syndrome, long-term clinical and radiologic follow-up is required for all patients with intracranial NGGCT.


Assuntos
Neoplasias Encefálicas/patologia , Lipídeos/análise , Teratoma/patologia , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/metabolismo , Pré-Escolar , Humanos , Metabolismo dos Lipídeos , Masculino , Pinealoma/diagnóstico , Pinealoma/metabolismo , Pinealoma/patologia , Teratoma/diagnóstico , Teratoma/metabolismo
3.
Acta Neuropathol ; 139(2): 243-257, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31768671

RESUMO

Tumors of the pineal region comprise several different entities with distinct clinical and histopathological features. Whereas some entities predominantly affect adults, pineoblastoma (PB) constitutes a highly aggressive malignancy of childhood with a poor outcome. PBs mainly arise sporadically, but may also occur in the context of cancer predisposition syndromes including DICER1 and RB1 germline mutation. With this study, we investigate clinico-pathological subgroups of pineal tumors and further characterize their biological features. We performed genome-wide DNA methylation analysis in 195 tumors of the pineal region and 20 normal pineal gland controls. Copy-number profiles were obtained from DNA methylation data; gene panel sequencing was added for 93 tumors and analysis was further complemented by miRNA sequencing for 22 tumor samples. Unsupervised clustering based on DNA methylation profiling separated known subgroups, like pineocytoma, pineal parenchymal tumor of intermediate differentiation, papillary tumor of the pineal region and PB, and further distinct subtypes within these groups, including three subtypes within the core PB subgroup. The novel molecular subgroup Pin-RB includes cases of trilateral retinoblastoma as well as sporadic pineal tumors with RB1 alterations, and displays similarities with retinoblastoma. Distinct clinical associations discriminate the second novel molecular subgroup PB-MYC from other PB cases. Alterations within the miRNA processing pathway (affecting DROSHA, DGCR8 or DICER1) are found in about two thirds of cases in the three core PB subtypes. Methylation profiling revealed biologically distinct groups of pineal tumors with specific clinical and molecular features. Our findings provide a foundation for further clinical as well as molecular and functional characterization of PB and other pineal tumors, including the role of miRNA processing defects in oncogenesis.


Assuntos
Neoplasias Encefálicas/genética , Neoplasias Encefálicas/patologia , Glândula Pineal , Pinealoma/genética , Pinealoma/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias Encefálicas/metabolismo , Estudos de Casos e Controles , Criança , Metilação de DNA , Feminino , Humanos , Masculino , MicroRNAs , Pessoa de Meia-Idade , Mutação/genética , Pinealoma/metabolismo , Adulto Jovem
5.
Appl Immunohistochem Mol Morphol ; 27(3): 210-215, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-28800009

RESUMO

INTRODUCTION: Pineal parenchymal tumor of intermediate differentiation (PPTID) is an uncommon tumor of the pineal gland. Although this behaves as a grade II/III tumor, the exact clinical behavior is not well known. There is no well-established pathologic factor that can predict the behavior of PPTID. AIM AND OBJECTIVE: The aim of this study was to determine the pathologic prognostic factors in PPTID. MATERIALS AND METHODS: All PPTID cases diagnosed between 2006 and 2016 were analyzed retrospectively. Immunohistochemistry for synaptophysin, neurofilament protein (NFP), glial fibrillar acid protein, NeuN, and Ki-67 were performed in all cases. Cases were classified arbitrarily into low grade (mitosis <4/10 hpf and Ki-67 <5%) and high grade (mitosis ≥4/10 hpf and Ki-67 ≥5%). Clinical details including follow-up information were retrieved from the patients' files. RESULTS: A total of 16 patients (6 low grade and 10 high grade) were included in this study. The age ranged from 2 to 55 years (average, 28.2) with a mild male preponderance (male:female, 1.67:1). All cases showed strong and diffuse positivity for synaptophysin. Focal NFP positivity was seen in 2 low-grade and 3 high-grade tumors. Only 2 cases showed focal NeuN positivity. Average Ki-67 index was 1.7% and 12.6% in low-grade and high-grade tumors, respectively. All patients with low-grade tumor were alive without recurrence. Among the patients with high-grade tumors, 2 had local recurrence, 1 had spinal metastasis, and 3 patients died. CONCLUSION: Mitosis and Ki-67 proliferation index are the most important pathologic prognostic factors in PPTID. NFP expression does not carry any prognostic significance.


Assuntos
Neoplasias Encefálicas , Proteínas de Neoplasias/biossíntese , Glândula Pineal , Pinealoma , Adolescente , Adulto , Neoplasias Encefálicas/diagnóstico , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patologia , Criança , Pré-Escolar , Feminino , Seguimentos , Humanos , Masculino , Pessoa de Meia-Idade , Glândula Pineal/metabolismo , Glândula Pineal/patologia , Pinealoma/diagnóstico , Pinealoma/metabolismo , Pinealoma/patologia , Prognóstico , Estudos Retrospectivos
7.
J Cancer Res Ther ; 14(Supplement): S806-S808, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30249910

RESUMO

Ectopic extrarenal renin-producing tumor is a rare disease with approximately 30 case reports in English literature. We herein present the first case of renin-producing germ cell tumors in the pineal apparatus and mediastinum. A 26-year-old man who had undergone craniotomy for the treatment of pineal tumor was found to have hypertension at a regular visit postoperatively. Laboratory findings revealed high plasma levels of renin activity and that of aldosterone concentration. Chest computed tomography demonstrated a large tumor in the mediastinum. The pathological findings revealed the mediastinal germ cell tumor positive for renin. The present case suggests that for young patients presenting with hypertension with a mediastinal tumor, the possibility of a renin-producing tumor should be considered.


Assuntos
Hipertensão/metabolismo , Neoplasias do Mediastino/metabolismo , Neoplasias Embrionárias de Células Germinativas/metabolismo , Pinealoma/metabolismo , Adulto , Aldosterona/metabolismo , Craniotomia , Humanos , Hipertensão/complicações , Hipertensão/diagnóstico por imagem , Hipertensão/patologia , Masculino , Neoplasias do Mediastino/complicações , Neoplasias do Mediastino/patologia , Neoplasias do Mediastino/cirurgia , Neoplasias Embrionárias de Células Germinativas/complicações , Neoplasias Embrionárias de Células Germinativas/diagnóstico por imagem , Neoplasias Embrionárias de Células Germinativas/cirurgia , Pinealoma/complicações , Pinealoma/diagnóstico por imagem , Pinealoma/cirurgia , Renina/metabolismo , Tomografia Computadorizada por Raios X
8.
J Neurooncol ; 140(2): 269-279, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30128689

RESUMO

PURPOSE: Medulloblastoma, the most common primary pediatric malignant brain tumor, originates in the posterior fossa of the brain. Pineoblastoma, which originates within the pineal gland, is a rarer malignancy that also presents in the pediatric population. Medulloblastoma and pineoblastoma exhibit overlapping clinical features and have similar histopathological characteristics. Histopathological similarities confound rapid diagnoses of these two tumor types. We have conducted a pilot feasibility study analyzing the molecular profile of archived frozen human tumor specimens using mass spectrometry imaging (MSI) to identify potential biomarkers capable of classifying and distinguishing between medulloblastoma and pineoblastoma. METHODS: We performed matrix-assisted laser desorption ionization Fourier transform ion cyclotron resonance mass spectrometry imaging on eight medulloblastoma biopsy specimens and three pineoblastoma biopsy specimens. Multivariate statistical analyses were performed on the MSI dataset to generate classifiers that distinguish the two tumor types. Lastly, the molecules that were discriminative of tumor type were queried against the Lipid Maps database and identified. RESULTS: In this pilot study we show that medulloblastoma and pineoblastoma can be discriminated using molecular profiles determined by MSI. The highest-ranking discriminating classifiers of medulloblastoma and pineoblastoma were glycerophosphoglycerols and sphingolipids, respectively. CONCLUSION: We demonstrate proof-of-concept that medulloblastoma and pineoblastoma can be rapidly distinguished by using MSI lipid profiles. We identified biomarker candidates capable of distinguishing these two histopathologically similar tumor types. This work expands the current molecular knowledge of medulloblastoma and pineoblastoma by characterizing their lipidomic profiles, which may be useful for developing novel diagnostic, prognostic and therapeutic strategies.


Assuntos
Neoplasias Encefálicas/metabolismo , Meduloblastoma/metabolismo , Pinealoma/metabolismo , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/patologia , Cerebelo/diagnóstico por imagem , Cerebelo/metabolismo , Criança , Diagnóstico Diferencial , Humanos , Meduloblastoma/diagnóstico por imagem , Meduloblastoma/patologia , Projetos Piloto , Glândula Pineal/diagnóstico por imagem , Glândula Pineal/metabolismo , Glândula Pineal/patologia , Pinealoma/diagnóstico por imagem , Pinealoma/patologia , Estudo de Prova de Conceito
9.
Am J Surg Pathol ; 41(10): 1410-1421, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28719464

RESUMO

Papillary tumor of the pineal region (PTPR) is a neuroepithelial neoplasm first described in 2003. Despite the anatomic association of PTPR with the pineal gland, the features of these tumors resemble those of the ependymal circumventricular subcommissural organ (SCO) of the posterior third ventricle. Given the presumed distinct derivation of PTPR and pineal parenchymal tumors, we hypothesized that expression of lineage-specific transcription factors could distinguish these tumors and provide additional insight into the differentiation of PTPR. A broad series of pineal region samples was reviewed, including 7 benign pineal glands, 4 pineal cysts, 13 pineocytomas, 28 pineal parenchymal tumors of intermediate differentiation, 11 pineoblastomas, and 18 PTPR. All samples were evaluated by immunohistochemistry for expression of CRX, a master transcriptional regulator of photoreceptor differentiation expressed in pineal gland and retina and/or FOXJ1, a master transcriptional regulator of ciliogenesis expressed in normal ependymal cells and ependymal neoplasms. Diffuse nuclear CRX expression is present in 100% of pineal samples. FOXJ1 is negative in all pineal samples. CRX staining is present in 53% of PTPR, though expression is nearly always limited to rare cells. Diffuse nuclear FOXJ1 expression is present in 100% of PTPR. Fetal human SCO diffusely expressed FOXJ1 but was negative for CRX. Immunohistochemistry for FOXJ1 and CRX differentiates non-germ cell pineal region tumors with high sensitivity and specificity, including pineal parenchymal tumors and PTPR. Our findings support the hypothesis that PTPR have ependymal differentiation and are phenotypically more similar to SCO than pineal gland.


Assuntos
Neoplasias Encefálicas/patologia , Fatores de Transcrição Forkhead , Proteínas de Homeodomínio , Glândula Pineal , Pinealoma/patologia , Transativadores , Neoplasias Encefálicas/química , Neoplasias Encefálicas/metabolismo , Diferenciação Celular , Núcleo Celular/metabolismo , Fatores de Transcrição Forkhead/análise , Fatores de Transcrição Forkhead/biossíntese , Proteínas de Homeodomínio/análise , Proteínas de Homeodomínio/biossíntese , Humanos , Pinealoma/química , Pinealoma/metabolismo , Transativadores/análise , Transativadores/biossíntese
10.
Brain Res ; 1642: 59-69, 2016 07 01.
Artigo em Inglês | MEDLINE | ID: mdl-27016056

RESUMO

The Unfolded Protein Response (UPR) is an adaptive cellular program used by eukaryotic cells to cope with protein misfolding stress in the Endoplasmic Reticulum (ER). During tumor development, cancer cells are facing intrinsic (oncogene activation) and extrinsic (limiting nutrient or oxygen supply; exposure to chemotherapies) challenges, with which they must cope to survive. Primary brain tumors are relatively rare but deadly and present a significant challenge in the determination of risk factors in the population. These tumors are inherently difficult to cure because of their protected location in the brain. As such surgery, radiation and chemotherapy options carry potentially lasting patient morbidity and incomplete tumor cure. Some of these tumors, such as glioblastoma, were reported to present features of ER stress and to depend on UPR activation to sustain growth, but to date there is no clear general representation of the ER stress status in primary brain tumors. In this review, we describe the key molecular mechanisms controlling the UPR and their implication in cancers. Then we extensively review the literature reporting the status of ER stress in various primary brain tumors and discuss the potential impact of such observation on patient stratification and on the possibility of developing appropriate targeted therapies using the UPR as therapeutic target.


Assuntos
Neoplasias Encefálicas/metabolismo , Estresse do Retículo Endoplasmático , Resposta a Proteínas não Dobradas , Animais , Neoplasias Cerebelares/metabolismo , Neoplasias do Plexo Corióideo/metabolismo , Glioblastoma/metabolismo , Humanos , Meduloblastoma/metabolismo , Neoplasias Meníngeas/metabolismo , Meningioma/metabolismo , Glândula Pineal/metabolismo , Pinealoma/metabolismo , Transdução de Sinais
11.
J Neurol Sci ; 363: 207-16, 2016 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-27000252

RESUMO

BACKGROUND: While most pineal cysts (PCs) are asymptomatic, some PCs are accompanied with symptoms of variable severity. We suggested that symptom severity in symptomatic patients with non-hydrocephalic PCs relates to venous compression causing central venous hypertension. This study explored whether possible magnetic resonance imaging (MRI) biomarkers of central venous hypertension could differentiate the severity of symptoms in individuals with non-hydrocephalic PCs. METHODS: The study included all individuals with PCs and MRI available for analysis followed conservatively within the department from 2003 to 2014. Severity of symptoms at follow-up were assessed from a questionnaire. Suggested MRI biomarkers indicative of central venous hypertension were explored, in addition to MRI measures of cyst size, aqueduct stenosis, and tectal compression. RESULTS: The study included 66 patients. As compared to the 27/66 patients (41%) with "None-Moderate" symptoms at follow-up, the 39/66 patients (59%) with "Much-Severe" symptoms presented with significantly altered indices of central venous hypertension (tectum-splenium-cyst ratio and indices of thalamic and periventricular edema). PC grading based on MRI biomarkers of central venous hypertension differentiated the severity of symptoms. CONCLUSION: The results indicate an association between severity of symptoms and MRI biomarkers of central venous hypertension in symptomatic individuals with non-hydrocephalic PCs.


Assuntos
Neoplasias Encefálicas/diagnóstico por imagem , Cistos do Sistema Nervoso Central/diagnóstico por imagem , Hipertensão/diagnóstico por imagem , Imageamento por Ressonância Magnética/métodos , Pinealoma/diagnóstico por imagem , Adulto , Neoplasias Encefálicas/epidemiologia , Neoplasias Encefálicas/metabolismo , Cistos do Sistema Nervoso Central/epidemiologia , Cistos do Sistema Nervoso Central/metabolismo , Pressão Venosa Central , Estudos de Coortes , Feminino , Seguimentos , Humanos , Hipertensão/epidemiologia , Hipertensão/metabolismo , Masculino , Pinealoma/epidemiologia , Pinealoma/metabolismo
12.
Mol Cell Biol ; 36(3): 438-51, 2016 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-26598601

RESUMO

The restoration of p53 has been suggested as a therapeutic approach in tumors. However, the timing of p53 restoration in relation to its efficacy during tumor progression still is unclear. We now show that the restoration of p53 in murine premalignant proliferating pineal lesions resulted in cellular senescence, while p53 restoration in invasive pineal tumors did not. The effectiveness of p53 restoration was not dependent on p19(Arf) expression but showed an inverse correlation with Mdm2 expression. In tumor cells, p53 restoration became effective when paired with either DNA-damaging therapy or with nutlin, an inhibitor of p53-Mdm2 interaction. Interestingly, the inactivation of p53 after senescence resulted in reentry into the cell cycle and rapid tumor progression. The evaluation of a panel of human supratentorial primitive neuroectodermal tumors (sPNET) showed low activity of the p53 pathway. Together, these data suggest that the restoration of the p53 pathway has different effects in premalignant versus invasive pineal tumors, and that p53 activation needs to be continually sustained, as reversion from senescence occurs rapidly with aggressive tumor growth when p53 is lost again. Finally, p53 restoration approaches may be worth exploring in sPNET, where the p53 gene is intact but the pathway is inactive in the majority of examined tumors.


Assuntos
Neoplasias Encefálicas/patologia , Senescência Celular , Tumores Neuroectodérmicos Primitivos/patologia , Glândula Pineal/patologia , Pinealoma/patologia , Proteína Supressora de Tumor p53/metabolismo , Animais , Neoplasias Encefálicas/genética , Neoplasias Encefálicas/metabolismo , Proliferação de Células , Deleção de Genes , Regulação Neoplásica da Expressão Gênica , Humanos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Invasividade Neoplásica/genética , Invasividade Neoplásica/patologia , Tumores Neuroectodérmicos Primitivos/genética , Tumores Neuroectodérmicos Primitivos/metabolismo , Glândula Pineal/metabolismo , Pinealoma/genética , Pinealoma/metabolismo , Proteínas Proto-Oncogênicas c-mdm2/genética , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Proteína Supressora de Tumor p53/genética
13.
Brain Pathol ; 26(2): 199-205, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26113311

RESUMO

Papillary tumor of the pineal region (PTPR) is a neuroepithelial brain tumor, which might pose diagnostic difficulties and recurs often. Little is known about underlying molecular alterations. We therefore investigated chromosomal copy number alterations, DNA methylation patterns and mRNA expression profiles in a series of 24 PTPRs. Losses of chromosome 10 were identified in all 13 PTPRs examined. Losses of chromosomes 3 and 22q (54%) as well as gains of chromosomes 8p (62%) and 12 (46%) were also common. DNA methylation profiling using Illumina 450k arrays reliably distinguished PTPR from ependymomas and pineal parenchymal tumors of intermediate differentiation. PTPR could be divided into two subgroups based on methylation pattern, PTPR group 2 showing higher global methylation and a tendency toward shorter progression-free survival (P = 0.06). Genes overexpressed in PTPR as compared with ependymal tumors included SPDEF, known to be expressed in the rodent subcommissural organ. Notable SPDEF protein expression was encountered in 15/19 PTPRs as compared with only 2/36 ependymal tumors, 2/19 choroid plexus tumors and 0/23 samples of other central nervous system (CNS) tumor entities. In conclusion, PTPRs show typical chromosomal alterations as well as distinct DNA methylation and expression profiles, which might serve as useful diagnostic tools.


Assuntos
Neoplasias Encefálicas/genética , Neoplasias Encefálicas/metabolismo , Glândula Pineal/metabolismo , Pinealoma/genética , Pinealoma/metabolismo , Adolescente , Adulto , Neoplasias Encefálicas/classificação , Neoplasias Encefálicas/patologia , Criança , Pré-Escolar , Neoplasias do Plexo Corióideo/classificação , Neoplasias do Plexo Corióideo/genética , Neoplasias do Plexo Corióideo/metabolismo , Neoplasias do Plexo Corióideo/patologia , Aberrações Cromossômicas , Metilação de DNA , Análise Mutacional de DNA , Intervalo Livre de Doença , Ependimoma/classificação , Ependimoma/genética , Ependimoma/metabolismo , Ependimoma/patologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Recidiva Local de Neoplasia , Glândula Pineal/patologia , Pinealoma/classificação , Pinealoma/patologia , Polimorfismo de Nucleotídeo Único , RNA Mensageiro/metabolismo
14.
Diagn Cytopathol ; 43(8): 642-5, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-25914033

RESUMO

It is well known that the histology of normal pineal gland may resemble not only pineal tumors but also gliomas, owing to its cellularity which is much greater than that of normal white or gray matter. Our recent experience with a case in which part of a normal gland was submitted for intraoperative consultation, together with the scarcity of cytologic descriptions, led us to perform a cyto-histologic correlation study. In addition to the intraoperative case, we collected five pineal glands from consecutive adult autopsies. During the squash procedure, we often noted the presence of calcified grains. Smears were hypercellular, distributed in tissue fibrillary fragments and as numerous single cells, with crystalline structures. Pineal gland cells (pineocytes) were large, round, epithelioid with ill-defined cytoplasms and moderate nuclear pleomorphism. Spindle cells with greater fibrillary quality were less common. One of the most remarkable findings seen in all cases was the presence of cytoplasmic pigment. Histological evaluation and immunohistochemical staining confirmed that the tissue was normal pineal gland. The histology showed a characteristic lobular aspect and frequent corpora arenacea. The pigment seen cytologically was also encountered in histology and corresponded to lipofuscin. Cytologic features of the pineal gland are peculiar when compared to other normal structures of the central nervous system. These features correlate closely with what is seen on histology. In an adequate clinical context, and in combination with frozen sections, cytology allows a specific recognition of the pineal gland during intraoperative pathologic consultations.


Assuntos
Neoplasias Encefálicas/diagnóstico , Lipofuscina/química , Pinealoma/diagnóstico , Adulto , Autopsia , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/patologia , Núcleo Celular/patologia , Citoplasma/metabolismo , Citoplasma/patologia , Diagnóstico Diferencial , Células Epitelioides/metabolismo , Células Epitelioides/patologia , Feminino , Histocitoquímica , Humanos , Masculino , Pessoa de Meia-Idade , Glândula Pineal/metabolismo , Glândula Pineal/patologia , Pinealoma/metabolismo , Pinealoma/patologia
15.
Neurochirurgie ; 61(2-3): 106-12, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-24439798

RESUMO

The present paper investigates the clinical picture and the different clinical signs that reveal pineal region tumors or appear during the course of the follow-up. Biological malignancy and tumor extension determine the semiology and its setting up mode. Typical endocrine signs, dominated by abnormal puberty development, are frequently a part of the clinical scene. Bifocal or ectopic localization in the hypothalamic-pituitary region is accompanied by other endocrine signs such as ante- or post-pituitary insufficiencies which occur several months or even years after the first neurological signs appear. Due to a mass syndrome and obstructive hydrocephalus, intracranial hypertension signs are frequent but unspecific. A careful ophthalmologic examination is essential to search upward gaze paralysis and other signs of the Parinaud's tetrad or pentad. Midbrain dysfunction, including extrinsic aqueduct stenosis, are also prevalent. Except for abnormal pubertal signs, hyper-melatoninemia (secretory tumors) or a-hypo-melatoninemia (tumors destructing pineal) generally remains dormant. Some patients present sleep problems such as narcolepsy or sleepiness during the daytime as well as behavioral problems. This suggests a hypothalamic extension rather than a true consequence of melatonin secretion anomalies. Similarly, some patients may present signs of a "pinealectomized" syndrome, including (cluster) headaches, tiredness, eventually responsive to melatonin.


Assuntos
Neoplasias Encefálicas/patologia , Hidrocefalia/patologia , Hipertensão Intracraniana/patologia , Glândula Pineal/patologia , Pinealoma/metabolismo , Neoplasias Encefálicas/diagnóstico , Feminino , Humanos , Hidrocefalia/complicações , Hipertensão Intracraniana/etiologia , Masculino , Sono/fisiologia , Síndrome
17.
Zhonghua Bing Li Xue Za Zhi ; 42(3): 186-90, 2013 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-23769439

RESUMO

OBJECTIVE: To study the clinicopathologic features of papillary tumor of the pineal region (PTPR). METHOD: Three hundred and eighty six cases of pineal region and posterior third ventricle tumors, two newborn and two adult pineal glands were analyzed by HE, PAS and immunohistochemistry of 16 antibodies (EnVision method). RESULTS: Five cases of PTPR were diagnosed with mixed papillary features and densely cellular areas, and included one recurrent case. In the papillary areas, the vessels were lined by one or several layers of cuboidal/columnar cells; the vessel wall was hyalinized. In the densely cellular areas, sheets or nests of tumor cells were seen. The tumor cells of these five cases were immunoreactive to CK, CK8/18, synaptophysin, MAP2, nestin, S-100, and vimentin. Four cases were immunoreactive to NSE and CgA; and 2 cases were immunoreactive to NF. All five cases were negative for EMA, CK5/6, CEA, and NeuN. Ki-67 labeling index ranged from 1% to 6%.Three patients were alive, and the recurrent one died. CONCLUSIONS: PTPR occurs in patients with over a wide age range, from children to adults, and is more commonly found in male than female. PTPR is composed of both papillary and solid areas, characterized by epithelial cytology, and needs to be differentiated from ependymoma. PTPR may originate from the specialized ependymocytes of the subcommissural organ. The prognostic factors are early diagnosis, complete surgical resection and radiotherapy.


Assuntos
Neoplasias Encefálicas/patologia , Carcinoma Papilar/patologia , Glândula Pineal , Adolescente , Adulto , Neoplasias Encefálicas/diagnóstico por imagem , Neoplasias Encefálicas/metabolismo , Neoplasias Encefálicas/radioterapia , Neoplasias Encefálicas/cirurgia , Carcinoma Papilar/diagnóstico por imagem , Carcinoma Papilar/metabolismo , Carcinoma Papilar/radioterapia , Carcinoma Papilar/cirurgia , Criança , Diagnóstico Diferencial , Ependimoma/metabolismo , Ependimoma/patologia , Feminino , Humanos , Imuno-Histoquímica , Queratina-18/metabolismo , Queratina-8/metabolismo , Queratinas/metabolismo , Masculino , Proteínas Associadas aos Microtúbulos/metabolismo , Nestina/metabolismo , Pinealoma/metabolismo , Pinealoma/patologia , Proteínas S100/metabolismo , Sinaptofisina/metabolismo , Tomografia Computadorizada por Raios X , Vimentina/metabolismo , Adulto Jovem
18.
Brain Pathol ; 23(1): 19-27, 2013 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-22672440

RESUMO

Pediatric embryonal brain tumors can be difficult to classify. Atypical teratoid rhabdoid tumors (ATRT) contain rhabdoid cells, while primitive neuroectodermal tumors (PNETs) are composed of "small round blue cells." Loss of INI1 is a common event in ATRT; therefore, we investigated if the loss of INI1 protein expression was also observed in central nervous system (CNS) PNET and pineoblastoma. A histological review of 42 CNS PNETs and six pineoblastomas was performed. INI1 expression was assessed by immunohistochemistry. Sequencing was performed on the mutational hotspots of INI1. INI1-immunonegative tumors were further investigated using fluorescence in situ hybridization. Epithelial membrane antigen (EMA) protein expression was assessed in six CNS PNETs to further define the phenotype. Five CNS PNETs without rhabdoid cell morphology were immuno-negative for both INI1 and EMA. Of these primary CNS PNET patients, three died <11 months postdiagnosis, which was dissimilar to the INI1-immunopositive primary CNS PNETs where 18/24 (75%) patients were alive 1 year postdiagnosis. We have identified a small subgroup of CNS PNETs which lack INI1 protein expression, but have no evidence of rhabdoid cell morphology. INI1 protein loss may occur through mechanisms other than gene deletion. INI1 immunohistochemistry should be performed for all CNS PNET cases.


Assuntos
Neoplasias Encefálicas/metabolismo , Proteínas Cromossômicas não Histona/metabolismo , Proteínas de Ligação a DNA/metabolismo , Tumores Neuroectodérmicos Primitivos/metabolismo , Fatores de Transcrição/metabolismo , Adolescente , Neoplasias Encefálicas/classificação , Criança , Pré-Escolar , Proteínas Cromossômicas não Histona/genética , Estudos de Coortes , Proteínas de Ligação a DNA/genética , Feminino , Humanos , Lactente , Masculino , Mucina-1/metabolismo , Mutação/genética , Tumores Neuroectodérmicos Primitivos/classificação , Glândula Pineal/patologia , Pinealoma/metabolismo , Análise Serial de Proteínas , Proteína SMARCB1 , Fatores de Transcrição/genética
19.
Brain Tumor Pathol ; 30(2): 93-8, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22622671

RESUMO

Papillary tumor of the pineal region (PTPR) is a recently recognized entity. We present the pathologic findings of two cases of PTPR as examples, and discuss the presence of cellular pleomorphism in these tumors. Patient 1 is a 48-year-old man with a pineal region mass. The tumor had unique biphasic patterns, papillary/pseudopapillary areas, and increased mitotic activity. Juxtaposed areas had marked pleomorphism, including nuclear enlargement, smudgy chromatin, nuclear pseudoinclusions, and cytoplasmic vacuolation. Mitoses were absent in these areas. Immunohistochemical staining revealed strong S100 expression. CAM 5.2 and CK18 were strongly positive in a patchy fashion. MIB1 labeling indices were high in classic PTPR regions but very low in pleomorphic areas. Patient 2 was a 35-year-old male with a pineal region tumor characterized by papillary architecture and overall cellular monotony, rare mitoses, and pleomorphism as a more isolated finding, with associated nuclear enlargement and crowding. S100 and CAM 5.2 labeling were present, and MIB1 labeling index was very low throughout the tumor. We discuss the pathologic and phenotypic features of PTPR. Variable pleomorphism may be present, reflected in size variation and nuclear hyperchromasia, but was not accompanied by increased proliferative activity in these cases, suggesting a degenerative phenomenon.


Assuntos
Carcinoma Papilar/patologia , Pinealoma/patologia , Adulto , Biomarcadores/metabolismo , Carcinoma Papilar/metabolismo , Humanos , Imuno-Histoquímica , Queratinas/metabolismo , Masculino , Pessoa de Meia-Idade , Pinealoma/metabolismo , Proteínas S100/metabolismo
20.
Clin Imaging ; 36(6): 702-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23153998

RESUMO

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Assuntos
Biomarcadores Tumorais/análise , Imageamento por Ressonância Magnética/métodos , Espectroscopia de Ressonância Magnética/métodos , Pinealoma/diagnóstico , Pinealoma/metabolismo , Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Imagem Molecular/métodos , Prótons , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Adulto Jovem
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