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Pathog Dis ; 74(2)2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26676260

RESUMO

Chlamydia trachomatis causes sexually transmitted diseases with infertility, pelvic inflammatory disease and neonatal pneumonia as complications. The duration of urogenital mouse models with the strict mouse pathogen C. muridarum addressing vaginal shedding, pathological changes of the upper genital tract or infertility is rather long. Moreover, vaginal C. trachomatis application usually does not lead to the complications feared in women. A fast-to-perform mouse model is urgently needed to analyze new antibiotics, vaccine candidates, immune responses (in gene knockout animals) or mutants of C. trachomatis. To complement the valuable urogenital model with a much faster and quantifiable screening method, we established an optimized lung infection model for the human intracellular bacterium C. trachomatis serovar D (and L2) in immunocompetent C57BL/6J mice. We demonstrated its usefulness by sensitive determination of antibiotic effects characterizing advantages and limitations achievable by early or delayed short tetracycline treatment and single-dose azithromycin application. Moreover, we achieved partial acquired protection in reinfection with serovar D indicating usability for vaccine studies, and showed a different course of disease in absence of complement factor C3. Sensitive monitoring parameters were survival rate, body weight, clinical score, bacterial load, histological score, the granulocyte marker myeloperoxidase, IFN-γ, TNF-α, MCP-1 and IL-6.


Assuntos
Antibacterianos/uso terapêutico , Vacinas Bacterianas/imunologia , Chlamydia trachomatis/efeitos dos fármacos , Chlamydia trachomatis/fisiologia , Pneumonia por Clamídia/tratamento farmacológico , Pneumonia por Clamídia/prevenção & controle , Interações Hospedeiro-Patógeno , Animais , Antibacterianos/farmacologia , Carga Bacteriana , Biópsia , Linhagem Celular , Pneumonia por Clamídia/microbiologia , Pneumonia por Clamídia/mortalidade , Complemento C3/genética , Complemento C3/imunologia , Citocinas/biossíntese , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Interações Hospedeiro-Patógeno/efeitos dos fármacos , Interações Hospedeiro-Patógeno/imunologia , Humanos , Imunoglobulina G/imunologia , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Pulmão/microbiologia , Pulmão/patologia , Camundongos , Camundongos Knockout , Peroxidase/metabolismo
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