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1.
PLoS One ; 15(1): e0219805, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31917824

RESUMO

Footrot is a worldwide economically important, painful, contagious bacterial foot disease of domestic and wild ungulates caused by Dichelobacter nodosus. Benign and virulent strains have been identified in sheep presenting with mild and severe lesions, respectively. However, in Alpine ibex (Capra ibex ibex), both strains have been associated with severe lesions. Because the disease is widespread throughout sheep flocks in Switzerland, a nationwide footrot control program for sheep focusing on virulent strains shall soon be implemented. The aim of this cross-sectional study was to estimate the nationwide prevalence of both strain groups of D. nodosus in four wild indigenous ruminant species and to identify potential susceptible wildlife maintenance hosts that could be a reinfection source for domestic sheep. During two years (2017-2018), interdigital swabs of 1,821 wild indigenous ruminant species (Alpine ibex, Alpine chamois (Rupicapra rupicapra), roe deer (Capreolus capreolus), red deer (Cervus elaphus)) were analysed by Real-Time PCR. Furthermore, observed interspecies interactions were documented for each sample. Overall, we report a low prevalence of D. nodosus in all four indigenous wild ruminants, for both benign (1.97%, N = 36, of which 31 red deer) and virulent (0.05%, N = 1 ibex) strains. Footrot lesions were documented in one ibex with virulent strains, and in one ibex with benign strains. Interspecific interactions involving domestic livestock occurred mainly with cattle and sheep. In conclusion, the data suggest that wild ungulates are likely irrelevant for the maintenance and spread of D. nodosus. Furthermore, we add evidence that both D. nodosus strain types can be associated with severe disease in Alpine ibex. These data are crucial for the upcoming nationwide control program and reveal that wild ruminants should not be considered as a threat to footrot control in sheep in this context.


Assuntos
Animais Selvagens/microbiologia , Doenças dos Bovinos/epidemiologia , Dichelobacter nodosus/patogenicidade , Pododermatite Necrótica dos Ovinos/epidemiologia , Doenças dos Ovinos/epidemiologia , Animais , Bovinos , Doenças dos Bovinos/patologia , Doenças dos Bovinos/transmissão , Estudos Transversais , Cervos/microbiologia , Dichelobacter nodosus/classificação , Dichelobacter nodosus/genética , Monitoramento Epidemiológico , Pododermatite Necrótica dos Ovinos/patologia , Pododermatite Necrótica dos Ovinos/transmissão , Cabras/microbiologia , Prevalência , Rupicapra/microbiologia , Ovinos/microbiologia , Doenças dos Ovinos/patologia , Doenças dos Ovinos/transmissão , Suíça/epidemiologia
2.
Schweiz Arch Tierheilkd ; 161(7): 465-472, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31298215

RESUMO

INTRODUCTION: Ovine footrot caused by Dichelobacter nodosus is a highly contagious and painful disease representing an economic as well as an animal welfare problem. In order to get more information on the infection dynamics, 26 lambs and 4 ewes enrolled in an in-contact infection trial were monitored over two weeks for the presence of D. nodosus-specific DNA. Two D. nodosus-positive ewes were housed together with 13 confirmed negative lambs. The control group consisted of another 13 lamb siblings and two confirmed D. nodosus-negative ewes. Every foot of all sheep was sampled seven times over the two weeks experiment period and subsequently analyzed for the presence of D. nodosus by quantitative real-time PCR. The control group was negative at the beginning and the end of the experiment and showed no clinical symptoms of footrot. The two positive ewes showed a high, but hundred fold differing level of virulent D. nodosus that remained constant over time with one of the ewes being also weakly positive for benign D. nodosus. All lambs of the infection group were positive for virulent D. nodosus at 14 days post infection (dpi). The first positive animals were observed on 3 dpi. The D. nodosus load remained at a low level and only increased in a few lambs at the end of the trial. Five of the contact lambs showed suspicious clincal signs (score 1-2) at 14 dpi corroborating the PCR results and indicating that the disease starts as early as two weeks after contact with positive sheep.


INTRODUCTION: Le piétin causé par Dichelobacter nodosus est une maladie hautement contagieuse et douloureuse qui représente à la fois un problème économique et de bien-être animal. Pour avoir plus informations sur la dynamique de l'infection, 26 agneaux et 4 brebis appartenant à un groupe d'essai d'infection par contact ont été contrôlés pendant deux semaines quant à la présence d'ADN spécifique de D. nodosus. Deux brebis positives pour D. nodosus ont été mises en contact avec 13 agneaux confirmés négatifs. Le groupe témoin était formé de 13 autres agneaux et deux brebis confirmées négatives. Sept échantillons sur écouvillon ont été prélevés sur chaque pied de chaque mouton au cours des deux semaines de la période expérimentale et analysés quant à la présence de D. nodosus par PCR quantitative en temps réel. Le groupe témoin était négatif au début et à la fin de l'expérience et n'a montré aucun symptôme clinique de piétin. Les deux brebis positives ont montré une forte présence de D. nodosus virulent, mais de cent niveaux différents, qui est restée constant dans le temps, l'une des brebis étant aussi faiblement positive pour D. nodosus bénin. Tous les agneaux du groupe infecté étaient positifs pour D. nodosus virulent 14 jours après l'infection (dpi). Les premiers animaux positifs ont été observés à 3 dpi. La charge de D. nodosus est restée faible et n'a augmenté que chez quelques agneaux à la fin de l'expérience. Cinq des agneaux en contact ont présenté des symptômes suspects (score 1-2) à 14 dpi, corroborant les résultats de la PCR et indiquant que l'infection commence dès deux semaines après le contact avec des moutons positifs.


Assuntos
Infecções por Bactérias Gram-Negativas/veterinária , Animais , Carga Bacteriana , Dichelobacter nodosus/fisiologia , Feminino , Pododermatite Necrótica dos Ovinos/diagnóstico , Pododermatite Necrótica dos Ovinos/epidemiologia , Pododermatite Necrótica dos Ovinos/microbiologia , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Negativas/transmissão , Reação em Cadeia da Polimerase , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/transmissão , Suíça/epidemiologia
3.
Vet Microbiol ; 218: 20-24, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29685216

RESUMO

Footrot caused by Dichelobacter nodosus is a highly contagious bacterial disease affecting the claw of sheep and the main cause of lameness in these animals. It is not only an economic burden but also a serious animal welfare issue. More information about the transmission of D. nodosus is needed for effective footrot control programs. We therefore determined the prevalence of D. nodosus in sheep presented at shows and markets where commingling of animals occurs. Furthermore, possible transmission vectors during foot trimming were investigated and trimming knife decontamination protocols evaluated. Sheep at six markets and four shows were sampled and tested for the presence of D. nodosus by real-time PCR. Different vectors, such as trimming knives were tested by real-time PCR and for viable D. nodosus by culture. The prevalence of virulent D. nodosus in sheep presented at shows and markets ranged from 1.7% to 100%. Regions with an ongoing control program showed significantly lower prevalence. After trimming, positive real-time PCR and culture results were obtained from the knives, the hands of the claw trimmers as well as removed claw horn material whereas boots were only positive by real-time PCR. In conclusion, markets and shows pose a risk for transmission of D. nodosus. The risk of transmission is particularly high during claw trimming and recommended measures to limit this risk include wiping the knife with a disinfection towel, wearing and changing gloves after every sheep, as well as proper disposal of trimmed and infectious horn.


Assuntos
Dichelobacter nodosus/isolamento & purificação , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/transmissão , Doenças dos Ovinos/transmissão , Animais , Dichelobacter nodosus/genética , Pododermatite Necrótica dos Ovinos/microbiologia , Pododermatite Necrótica dos Ovinos/prevenção & controle , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/prevenção & controle , Casco e Garras/microbiologia , Coxeadura Animal/etiologia , Coxeadura Animal/microbiologia , Prevalência , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Ovinos , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/prevenção & controle
4.
Prev Vet Med ; 146: 86-93, 2017 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-28992932

RESUMO

In 2008, virulent footrot was detected in sheep in south-west Norway. Footrot is caused by Dichelobacter nodosus, and the outbreak was linked to live sheep imported from Denmark in 2005. A large-scale program for eradicating the disease was implemented as a joint industry and governmental driven eradication project in the years 2008-2014, and continued with surveillance and control measures by the Norwegian Food Safety Authority from 2015. The cost of the eradication program including surveillance and control measures until 2032 was assumed to reach approximately €10.8 million (NOK 90 million). A financial cost-benefit analysis, comparing costs in the eradication program with costs in two simulated scenarios, was carried out. In the scenarios, designated ModerateSpread (baseline) and SlowSpread, it was assumed that the sheep farmers would undertake some voluntary measures on their own that would slow the spread of the disease. The program obtained a positive NPV after approximately 12 years. In a stochastic analysis, the probabilities of a positive NPV were estimated to 1.000 and to 0.648 after 15 years and to 0.378 and 0.016 after ten years, for the ModerateSpread and SlowSpread scenarios respectively. A rapid start-up of the program soon after the detection of the disease was considered crucial for the economic success as the disease would have become more widespread and probably raised the costs considerably at a later start-up.


Assuntos
Controle de Doenças Transmissíveis/economia , Pododermatite Necrótica dos Ovinos/economia , Pododermatite Necrótica dos Ovinos/prevenção & controle , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/economia , Doenças dos Ovinos/prevenção & controle , Animais , Controle de Doenças Transmissíveis/métodos , Análise Custo-Benefício , Dinamarca , Dichelobacter nodosus/isolamento & purificação , Surtos de Doenças/economia , Surtos de Doenças/prevenção & controle , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/economia , Infecções por Bactérias Gram-Negativas/prevenção & controle , Infecções por Bactérias Gram-Negativas/transmissão , Seguro/economia , Modelos Econométricos , Noruega , Vigilância de Evento Sentinela/veterinária , Ovinos , Doenças dos Ovinos/transmissão , Processos Estocásticos
6.
Acta Vet Scand ; 57: 55, 2015 Sep 25.
Artigo em Inglês | MEDLINE | ID: mdl-26407552

RESUMO

BACKGROUND: Dichelobacter nodosus is the main causative agent of ovine footrot, and there are strong indications that the bacterium can be transferred to cattle grazing on the same pasture as sheep. The aim of this study was to investigate if benign and virulent D. nodosus strains isolated from sheep can be transferred to the interdigital skin of cattle under experimental conditions. Further, we wanted to observe the impact of such infection on bovine foot health, and test the effect of topical chlortetracycline (Cyclo spray(®): Eurovet) on the infection. FINDINGS: Six heifers were included in the study. After an initial 18-day maceration period, three heifers were inoculated on one single foot with a benign strain and three with a virulent strain by adding bacterial suspension in a bandage. The bandages were left on for 17 days, and when removed, D. nodosus was isolated from all six heifers. All six heifers developed interdigital dermatitis. In five of the heifers D. nodosus organisms were demonstrated within the epidermis. Twenty-four days after treatment with chlortetracycline all heifers were negative by cultivation, but tested positive for D. nodosus by polymerase chain reaction (PCR). Two of the six heifers still tested positive for D. nodosus by PCR 49 days after treatment. After 70 days, all heifers tested negative for D. nodosus. CONCLUSIONS: This study shows that both virulent and benign D. nodosus strains originating from sheep can be transferred to naïve heifers under experimental conditions. Further, the study supports the hypothesis that infections with virulent D. nodosus in cattle are associated with interdigital dermatitis. No conclusion regarding the treatment of D. nodosus infection with chlortetracycline was possible.


Assuntos
Antibacterianos/uso terapêutico , Doenças dos Bovinos/microbiologia , Clortetraciclina/uso terapêutico , Dichelobacter nodosus/fisiologia , Dermatite Digital/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Animais , Bovinos , Doenças dos Bovinos/tratamento farmacológico , Doenças dos Bovinos/transmissão , Dermatite Digital/tratamento farmacológico , Dermatite Digital/transmissão , Feminino , Pododermatite Necrótica dos Ovinos/microbiologia , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/tratamento farmacológico , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/transmissão , RNA Ribossômico 16S/genética , Reação em Cadeia da Polimerase em Tempo Real/veterinária , Ovinos , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/transmissão , Carneiro Doméstico
7.
Vet Microbiol ; 179(1-2): 53-9, 2015 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-25953734

RESUMO

Dichelobacter nodosus (D. nodosus) is the essential causative agent of footrot in sheep. The current study investigated when D. nodosus was detectable on newborn lambs and possible routes of transmission. Specific qPCR was used to detect and quantify the load of D. nodosus in foot swabs of lambs at birth and 5-13 h post-partum, and their mothers 5-13 h post-partum; and in samples of bedding, pasture, soil and faeces. D. nodosus was not detected on the feet of newborn lambs swabbed at birth, but was detected 5-13 h after birth, once they had stood on bedding containing naturally occurring D. nodosus. Multiple genotypes identified by cloning and sequencing a marker gene, pgrA, and by multi locus variable number tandem repeat analysis (MLVA) of community DNA from swabs on individual feet indicated a mixed population of D. nodosus was present on the feet of both ewes and lambs. There was high variation in pgrA tandem repeat number (between 3 and 21 repeats), and multiple MLVA types. The overall similarity index between the populations on ewes and lambs was 0.45, indicating moderate overlap. Mother offspring pairs shared some alleles but not all, suggesting lambs were infected from sources(s) other than just their mother's feet. We hypothesise that D. nodosus is transferred to the feet of lambs via bedding containing naturally occurring populations of D. nodosus, probably as a result of transfer from the feet of the group of housed ewes. The results support the hypothesis that the environment plays a key role in the transmission of D. nodosus between ewes and lambs.


Assuntos
Dichelobacter nodosus/isolamento & purificação , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/transmissão , Animais , Animais Recém-Nascidos , Sequência de Bases , DNA Bacteriano/química , DNA Bacteriano/genética , Dichelobacter nodosus/genética , Feminino , Pododermatite Necrótica dos Ovinos/microbiologia , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/transmissão , Repetições Minissatélites/genética , Dados de Sequência Molecular , Gravidez , Análise de Sequência de DNA/veterinária , Ovinos , Doenças dos Ovinos/microbiologia , Microbiologia do Solo
8.
Vet Res ; 46: 10, 2015 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-25828795

RESUMO

When severe footrot was detected in Norway in 2008, a surveillance programme was initiated and followed by an elimination programme. By 2013 the disease had spread to two of 19 counties and a total of 119 (1%) sheep flocks had been diagnosed with severe footrot. A simulation model was developed to estimate the potential spread of severe footrot in Norway and to estimate the relative importance of the different spreading routes. The model parameters were based on the rate of spread of the first 38 diagnosed cases and the management and climatic factors particular for Norway. The model showed that by 2013, severe footrot would have spread to six counties and infected 16% of the sheep flocks if no elimination programme had been initiated. If this is compared with the 1% of flocks that were diagnosed in Norway by 2013, there seems to be a large effect of the implemented footrot elimination programme. By 2035, it was estimated that severe footrot would have spread to 16 counties and 64% of the sheep flocks. Such an extensive spread would probably impose a large negative impact on the sheep industry and welfare of the sheep. The most effective way to curb the spread of severe footrot was by decreasing the within county infection rate. This could be achieved by decreasing the contact between flocks or by decreasing the environmental load of D. nodosus, for example by footbathing sheep, culling diseased sheep or eliminating severe footrot in the flock.


Assuntos
Dichelobacter nodosus/fisiologia , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/transmissão , Animais , Clima , Simulação por Computador , Pododermatite Necrótica dos Ovinos/microbiologia , Pododermatite Necrótica dos Ovinos/prevenção & controle , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/prevenção & controle , Infecções por Bactérias Gram-Negativas/transmissão , Modelos Teóricos , Noruega , Ovinos , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/prevenção & controle
9.
Vet Microbiol ; 170(3-4): 375-82, 2014 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-24698131

RESUMO

Dichelobacter nodosus is the main aetiological agent of ovine footrot and the bacterium has also been associated with interdigital dermatitis is cattle. The aim of this study was to investigate possible cross-infection of virulent D. nodosus between sheep and co-grazing cattle. Five farms, where sheep previously diagnosed with virulent D. nodosus were co-grazing with cattle for different periods of time, were included. The study sample consisted of 200 cows and 50 sheep. All cows were examined for the presence of interdigital dermatitis, and ten ewes, preferably with symptoms of footrot, had the footrot scores recorded. On each farm, the same ten ewes and ten cows were chosen for bacterial analyses. Swabs were analysed for D. nodosus by PCR and culturing. D. nodosus isolates were virulence-tested and assigned to serogroups by fimA variant determination. Biopsies were evaluated histopathologically and analysed by fluorescent in situ hybridization for D. nodosus, Treponema spp. and Fusobacterium necrophorum. D. nodosus defined as virulent by the gelatin gel test were isolated from 16 sheep from four farms and from five cows from two of the same farms. All five cows had interdigital dermatitis. Two of the cows stayed infected for at least eight months. By pulsed-field gel electrophoresis (PFGE), the isolates from the five cows were found to be genetically indistinguishable or closely related to isolates from sheep from the same farm. This indicates that cross-infection between sheep and cows have occurred.


Assuntos
Doenças dos Bovinos/transmissão , Dichelobacter nodosus/fisiologia , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/transmissão , Animais , Bovinos , Doenças dos Bovinos/microbiologia , Doenças dos Bovinos/patologia , Dichelobacter nodosus/genética , Dichelobacter nodosus/isolamento & purificação , Eletroforese em Gel de Campo Pulsado , Pododermatite Necrótica dos Ovinos/microbiologia , Pododermatite Necrótica dos Ovinos/patologia , Infecções por Fusobacterium/veterinária , Fusobacterium necrophorum , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/transmissão , Reação em Cadeia da Polimerase , Ovinos , Doenças dos Ovinos/microbiologia , Doenças dos Ovinos/patologia , Treponema , Infecções por Treponema/veterinária
10.
Acta Vet Scand ; 54: 19, 2012 Mar 29.
Artigo em Inglês | MEDLINE | ID: mdl-22458248

RESUMO

BACKGROUND: The aim of this study was to investigate possible cross-infection of Dichelobacter nodosus in Norwegian farms practising co-grazing of sheep and cattle. METHODS: Thirteen farms practising co-grazing of sheep and cattle were included in this descriptive study: five farms with a history of severe ovine footrot (Group I) and eight farms with free-stall housing of cattle and signs of mild or no footrot in sheep (Group II). Sampling for PCR detection of D. nodosus was performed from animals in all farms, and clinical claw examination of sheep and cattle was performed in Group II. D. nodosus positive samples were analysed by a multiplex PCR method that detects variants of the fimA gene corresponding to D. nodosus serogroups A through I. RESULTS: D. nodosus serogroup A was identified more frequently in sheep from farms with a history of severe footrot (Group I) versus from Group II, and in most of the farms with a history of severe footrot there was a coexistence of D. nodosus serogroup A in sheep and cattle. In one farm heel horn erosion and dermatitis emerged in cattle after co-grazing with sheep suffering from severe footrot where D. nodosus serogroup A was detected. Six months later heel horn erosion and dermatitis were still diagnosed, and D. nodosus serogroup A was identified. Out of the 16 D. nodosus positive sheep samples from Group II, ten of the samples were positive by the fimA serogrouping PCR. Among these 10 samples all serogroups except G were detected. All the D. nodosus serogroups detected in sheep were also present in the corresponding cattle herds. CONCLUSION: The clinical findings and the coexistence of the same serogroups in co-grazing sheep and cattle could indicate cross-infection. However, further research including isolation of the bacterial strains, virulence-testing and genetic identification, is needed.


Assuntos
Doenças dos Bovinos/microbiologia , Infecção Hospitalar/veterinária , Dichelobacter nodosus/isolamento & purificação , Pododermatite Necrótica dos Ovinos/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/microbiologia , Animais , Bovinos , Doenças dos Bovinos/transmissão , Infecção Hospitalar/microbiologia , DNA Bacteriano/química , DNA Bacteriano/genética , Dichelobacter nodosus/genética , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/transmissão , Noruega , Reação em Cadeia da Polimerase/veterinária , Ovinos , Doenças dos Ovinos/transmissão
11.
Genetics ; 188(3): 683-93, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21527777

RESUMO

Previous studies have shown that host genetic heterogeneity in the response to infectious challenge can affect the emergence risk and the severity of diseases transmitted through direct contact between individuals. However, there is substantial uncertainty about the degree and direction of influence owing to different definitions of genetic variation, most of which are not in line with the current understanding of the genetic architecture of disease traits. Also, the relevance of previous results for diseases transmitted through environmental sources is unclear. In this article a compartmental genetic-epidemiological model was developed to quantify the impact of host genetic diversity on epidemiological characteristics of diseases transmitted through a contaminated environment. The model was parameterized for footrot in sheep. Genetic variation was defined through continuous distributions with varying shape and degree of dispersion for different disease traits. The model predicts a strong impact of genetic heterogeneity on the disease risk and its progression and severity, as well as on observable host phenotypes, when dispersion in key epidemiological parameters is high. The impact of host variation depends on the disease trait for which variation occurs and on environmental conditions affecting pathogen survival. In particular, compared to homogeneous populations with the same average susceptibility, disease risk and severity are substantially higher in populations containing a large proportion of highly susceptible individuals, and the differences are strongest when environmental contamination is low. The implications of our results for the recording and analysis of disease data and for predicting response to selection are discussed.


Assuntos
Pododermatite Necrótica dos Ovinos/genética , Variação Genética , Genética Populacional/métodos , Algoritmos , Animais , Dichelobacter nodosus/crescimento & desenvolvimento , Meio Ambiente , Pododermatite Necrótica dos Ovinos/epidemiologia , Pododermatite Necrótica dos Ovinos/transmissão , Predisposição Genética para Doença , Genética Populacional/estatística & dados numéricos , Genótipo , Modelos Genéticos , Modelos Teóricos , Fenótipo , Prevalência , Probabilidade , Fatores de Risco , Índice de Gravidade de Doença , Ovinos/microbiologia
12.
Vet Res Commun ; 33(7): 749-55, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19449113

RESUMO

To analyze immunodominant regions of leukotoxin protein of Fusobacterium necrophorum strain H05, a series of truncated forms of leukotoxin gene were expressed in Escherichia coli using the vector pGEX-6p-1 or pPROEX HTa. The results of SDS-PAGE showed the truncated forms PL1, PL2, PL4, and PL5 were expressed in Escherichia coli using the vector pGEX-6p-1, and the truncated forms PL3 was expressed in Escherichia coli using the vector pPROEX HTa. These recombinant proteins were able to react with antisera against Fusobacterium necrophorum strain A25. In five recombinant proteins, the recombinant proteins PL1, PL3 and PL4 as vaccine were able to elicit formation of the better protective effects on mice against infection of Fusobacterium necrophorum strain A25.


Assuntos
Toxinas Bacterianas/imunologia , Exotoxinas/imunologia , Pododermatite Necrótica dos Ovinos/imunologia , Fusobacterium/imunologia , Imunossupressores/imunologia , Animais , Proteínas de Bactérias/química , Proteínas de Bactérias/genética , Proteínas de Bactérias/imunologia , Proteínas de Bactérias/uso terapêutico , Toxinas Bacterianas/química , Citotoxinas/química , Citotoxinas/imunologia , Primers do DNA , Escherichia coli/genética , Exotoxinas/química , Exotoxinas/genética , Pododermatite Necrótica dos Ovinos/prevenção & controle , Pododermatite Necrótica dos Ovinos/transmissão , Fusobacterium/genética , Fusobacterium/patogenicidade , Vetores Genéticos , Proteínas Hemolisinas/química , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/imunologia , Proteínas Hemolisinas/uso terapêutico , Imunossupressores/química , Proteínas Recombinantes/imunologia , Ruminantes , Virulência
13.
Vet J ; 171(3): 421-8, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16624707

RESUMO

Footrot is a highly contagious disease of the feet of ruminants caused by the synergistic action of certain bacterial species of which Dichelobacter nodosus (D. nodosus) is the main transmitting agent. The infection is specific to sheep and goats, although it has also been reported in cattle, horses, pigs, deer and mouflon. The antigenic diversity of D. nodosus is due to variations in the DNA sequence of its fimbrial subunit gene (fimA) and provides the basis for classification of the organism into at least 10 major serogroups (A-I and M), the distribution of which varies with different geographical locations. Host immune response to vaccination is serogroup specific. There are three different clinical forms of disease caused by virulent, intermediate and benign strains of D. nodosus, respectively. In order to facilitate rapid and reliable clinical diagnosis, virulence determination, strain differentiation and serogroup identification for effective control measures, immunological tests, DNA probes and PCR based techniques have been introduced. This review summarises the current understanding of the mechanisms of antigenic diversity of D. nodosus as well as advances made in its strain differentiation and diagnosis.


Assuntos
Variação Antigênica , Dichelobacter nodosus/genética , Pododermatite Necrótica dos Ovinos/microbiologia , Doenças das Cabras/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/microbiologia , Animais , Eletroforese em Gel de Campo Pulsado , Pododermatite Necrótica dos Ovinos/diagnóstico , Pododermatite Necrótica dos Ovinos/transmissão , Doenças das Cabras/diagnóstico , Doenças das Cabras/transmissão , Cabras , Infecções por Bactérias Gram-Negativas/diagnóstico , Infecções por Bactérias Gram-Negativas/microbiologia , Infecções por Bactérias Gram-Negativas/transmissão , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Ovinos , Doenças dos Ovinos/diagnóstico , Doenças dos Ovinos/transmissão
14.
Aust Vet J ; 78(4): 273-6, 2000 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-10840576

RESUMO

OBJECTIVE: To investigate the occurrence of S1, U1 and T strains of Dichelobacter nodosus in new clinical lesions in sheep exposed to footrot associated with multi-strain infections. DESIGN: Seventy-seven donor sheep were grazed with 84 recipients for 33 weeks. The donor sheep were Merinos with a history of clinically virulent footrot associated with protease type S1, U1 and T strains of D nodosus that hybridised with gene sequences pJIR314B, pJIR318 and/or pB645-335. The recipient sheep were Merinos with no history of footrot. PROCEDURE: Each fortnight, all feet were examined, their lesion scores were recorded and samples of lesion material were taken for laboratory tests. RESULTS: Eighty-nine percent (299 of 336) of feet of recipient sheep developed new clinical lesions. S1, U1 and T strains of D nodosus were recovered from 58%, 22% and 18%, respectively, of these lesions at a ratio that remained constant during two apparent peaks in footrot transmission. Gene sequences homologous to pJIR314B and pB645-335 were detected in 56% (93 of 166) and 29% (48 of 166), respectively, of S1 strains of D nodosus at a ratio that was not constant during the experiment. CONCLUSIONS: S1 was the dominant protease type of D nodosus in new clinical lesions. The occurrence of S1 strains did not increase relative to U1 and T strains of D nodosus during the experiment. S1, U1 and T strains of D nodosus remained in equilibrium despite changes in environment, genetic types in the population of S1 strains, and host resistance to footrot.


Assuntos
Dichelobacter nodosus/classificação , Pododermatite Necrótica dos Ovinos/microbiologia , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/microbiologia , Animais , Dichelobacter nodosus/genética , Feminino , Pododermatite Necrótica dos Ovinos/epidemiologia , Pododermatite Necrótica dos Ovinos/transmissão , Infecções por Bactérias Gram-Negativas/epidemiologia , Infecções por Bactérias Gram-Negativas/transmissão , Masculino , Reação em Cadeia da Polimerase/veterinária , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/transmissão , Austrália Ocidental/epidemiologia
15.
Aust Vet J ; 77(7): 450-3, 1999 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10451731

RESUMO

OBJECTIVE: To determine the infectivity of ovine and caprine strains of Dichelobacter nodosus for both sheep and goats. DESIGN: Pen experiments in which 20 sheep and 19 goats were challenged directly with the two strains, and transmission experiments on pasture, using donors infected by experimental challenge. RESULTS: Sheep and goat strains of D nodosus infected both animal species in experimental challenges. Animals so infected transmitted footrot to both sheep and goats on pasture plots. A significantly smaller proportion of goats than sheep was infected when challenged with either strain. The interval between exposure and development of footrot in goats was longer than in sheep when recipient animals were exposed to infected donors on pasture. The disease was less invasive in goats than in sheep. CONCLUSIONS: With the strains of D nodosus used there was no evidence of host specificity. Direct transmission of footrot can occur between sheep and goats in the same environment. There is a need to include goats in ovine footrot eradication programs and vice versa.


Assuntos
Dichelobacter nodosus/patogenicidade , Transmissão de Doença Infecciosa/veterinária , Pododermatite Necrótica dos Ovinos/transmissão , Doenças das Cabras/transmissão , Infecções por Bactérias Gram-Negativas/veterinária , Doenças dos Ovinos/transmissão , Animais , Animais Domésticos , Animais Selvagens , Cabras , Infecções por Bactérias Gram-Negativas/transmissão , Masculino , Reação em Cadeia da Polimerase/veterinária , Polimorfismo de Fragmento de Restrição , Índice de Gravidade de Doença , Ovinos
16.
Aust Vet J ; 72(4): 132-4, 1995 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-7646377

RESUMO

Virulent ovine footrot was transmitted accidentally to a group of 23 adult Merino sheep (flock B) after holding for 1 hour in sheep yards, which earlier the same day had contained another flock (flock A) with < 1% prevalence of sheep with footrot lesions. Sheep in flock B were rendered susceptible to virulent footrot by grazing 600 mm high unimproved pasture dominated by paspalum (Paspalum dilatatum) and kangaroo grass (Themeda australis) during warm, humid and wet weather. In addition to moisture, interdigital abrasions caused by the pasture might have predisposed the interdigital skin to infection with Dichelobacter nodosus.


Assuntos
Criação de Animais Domésticos , Bacteroides/patogenicidade , Transmissão de Doença Infecciosa/veterinária , Pododermatite Necrótica dos Ovinos/transmissão , Doenças dos Ovinos/transmissão , Animais , Bacteroides/classificação , Bacteroides/isolamento & purificação , Feminino , Pododermatite Necrótica dos Ovinos/microbiologia , Masculino , Prevalência , Sorotipagem/veterinária , Ovinos , Doenças dos Ovinos/microbiologia , Pele/microbiologia , Virulência , Tempo (Meteorologia)
18.
Aust Vet J ; 67(3): 98-101, 1990 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-2375715

RESUMO

Soluble outer membrane protein of Bacteroides nodosus extracted with potassium thiocyanate (KSCN) was employed as antigen in an enzyme linked immunosorbent assay (ELISA) to detect serum antibody in sheep naturally infected with a heterologous serogroup. Serum antibody responses in 55 sheep were monitored for 2 years and maximum levels were directly related to the severity of clinical foot lesions. Serum antibody levels rose 2 weeks after foot lesions developed and declined within several months of resolution of lesions. After the first footrot transmission period, antibody levels persisted significantly (P less than 0.001) longer in sheep that did not become affected in the next transmission period compared with sheep in which footrot recurred. Antibody response did not appear to result in resolution of foot lesions. ELISA using KSCN antigen gave similar results to whole cell ELISA where cells prepared from an homologous serogroup were used as antigen. Both these assays were more sensitive than ELISA in which heterologous whole cell antigen was used. Proteins extracted from the outer membrane of B. nodosus, which are known to be immunogenic in natural infection and common to different serogroups of B. nodosus, appear to be useful antigens for serological investigations of ovine footrot.


Assuntos
Anticorpos Antibacterianos/biossíntese , Infecções por Bacteroides/veterinária , Bacteroides/imunologia , Pododermatite Necrótica dos Ovinos/imunologia , Doenças dos Ovinos/imunologia , Animais , Proteínas da Membrana Bacteriana Externa/imunologia , Infecções por Bacteroides/imunologia , Infecções por Bacteroides/patologia , Infecções por Bacteroides/transmissão , Ensaio de Imunoadsorção Enzimática , Pododermatite Necrótica dos Ovinos/patologia , Pododermatite Necrótica dos Ovinos/transmissão , Casco e Garras/patologia , Masculino , Recidiva , Estudos Retrospectivos , Ovinos , Doenças dos Ovinos/patologia , Doenças dos Ovinos/transmissão
20.
Res Vet Sci ; 24(3): 300-4, 1978 May.
Artigo em Inglês | MEDLINE | ID: mdl-674842

RESUMO

Following reports of findings of ovine foot-rot flora in the feet of cattle, a study was undertaken to determine the prevalence of Bacteroides nodosus infection in the apparently normal cattle population. We found that 34.5 to 74.2 per cent of the animals examined on different farms had B nodosus present in one or more feet. B nodosus was not the most prevalent bacterium observed in smears from cattle. Other Gram negative species including Fusiformis necrophorus and many Gram positive cocci and coccobacilli were also present. Macroscopic lesions in the interdigital skin characterised by erosion and hyperkeratosis were usually associated with the occurrence of B nodosus. B nodosus isolated from cattle induced mild interdigital dermatitis in experimental cattle and sheep and the infection was transmitted to recipient cattle and sheep under field conditions. Virulent foot-rot of sheep was not transmitted to recipient cattle in conditions where the disease spread to susceptible sheep.


Assuntos
Infecções por Bacteroides/veterinária , Doenças dos Bovinos/epidemiologia , Animais , Austrália , Infecções por Bacteroides/epidemiologia , Infecções por Bacteroides/transmissão , Bovinos , Doenças dos Bovinos/transmissão , Pododermatite Necrótica dos Ovinos/epidemiologia , Pododermatite Necrótica dos Ovinos/transmissão
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