Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
J Craniofac Surg ; 31(7): 2059-2062, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-33003056

RESUMO

BACKGROUND: Polydioxanone (PDS) has been widely used in the medical field over the past 30 years. In the 2000s, PDS plate began to be used for rhinoplasty and septoplasty. However, in Asia PDS plates are not widely used due to lack of awareness and high prices. The authors devised a method of producing a modified PDS (m-PDS; Rhinoblock Material & Design Co., Gyeonggi-do, Sothh Korea) at low cost, and compared the biocompatibilities and degradabilities of plates produced with m-PDS and commercial PDS plates (Ethicon, Somerville, NJ) in vivo and in vitro. METHODS: The melting point and decomposition rate of m-PDS were determined by differential scanning calorimetry and thermogravimetric analysis and its tensile strength was also measured. Implants (1 cm × 1 cm × 0.15 mm sized) were inserted subcutaneously into mice and harvested en bloc 2, 5, 10, 15, or 25 weeks later. Tissues were stained with hematoxylin and eosin or Masson's trichrome to evaluate inflammation, extracellular matrix deposition, and vascularization, and plate degradability was also assessed. RESULTS: No significant difference was observed between the thermal analysis and tensile test results of m-PDS and PDS plates. m-PDS started to degrade in vivo from around 10 weeks, and commercial PDS plates from around 15 weeks. After 25 weeks in vivo, both products were completely degraded and not observed in tissue slides. Histologic analysis of excised specimens showed m-PDS and PDS were similar in terms of inflammation, extracellular matrix deposition, and vascularization. CONCLUSION: In vivo and in vitro experiments detected no significant difference between the biocompatibilities and degradabilities of modified and commercial PDS plates. The results of this study suggest that the modified PDS can be used to produce versatile, low cost, absorbable graft materials for rhinoplasty and septoplasty.


Assuntos
Polidioxanona/metabolismo , Animais , Ásia , Placas Ósseas , Inflamação/induzido quimicamente , Masculino , Teste de Materiais , Camundongos , Camundongos Endogâmicos C57BL , Polidioxanona/química , Polidioxanona/toxicidade , República da Coreia , Rinoplastia , Resistência à Tração
2.
J Dent Res ; 92(11): 963-9, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-24056225

RESUMO

Here we report the synthesis, materials characterization, antimicrobial capacity, and cytocompatibility of novel antibiotic-containing scaffolds. Metronidazole (MET) or Ciprofloxacin/(CIP) was mixed with a polydioxanone (PDS)polymer solution at 5 and 25 wt% and processed into fibers. PDS fibers served as a control. Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), tensile testing, and high-performance liquid chromatography (HPLC) were used to assess fiber morphology, chemical structure, mechanical properties, and drug release, respectively. Antimicrobial properties were evaluated against those of Porphyromonas gingivalis/Pg and Enterococcus faecalis/Ef. Cytotoxicity was assessed in human dental pulp stem cells (hDPSCs). Statistics were performed, and significance was set at the 5% level. SEM imaging revealed a submicron fiber diameter. FTIR confirmed antibiotic incorporation. The tensile values of hydrated 25 wt% CIP scaffold were significantly lower than those of all other groups. Analysis of HPLC data confirmed gradual, sustained drug release from the scaffolds over 48 hrs. CIP-containing scaffolds significantly (p < .00001) inhibited biofilm growth of both bacteria. Conversely, MET-containing scaffolds inhibited only Pg growth. Agar diffusion confirmed the antimicrobial properties against specific bacteria for the antibiotic-containing scaffolds. Only the 25 wt% CIP-containing scaffolds were cytotoxic. Collectively, this study suggests that polymer-based antibiotic-containing electrospun scaffolds could function as a biologically safe antimicrobial drug delivery system for regenerative endodontics.


Assuntos
Materiais Biocompatíveis/química , Regeneração Tecidual Guiada/métodos , Nanofibras/química , Tratamento do Canal Radicular/métodos , Alicerces Teciduais/química , Anti-Infecciosos/química , Anti-Infecciosos/farmacologia , Anti-Infecciosos/toxicidade , Materiais Biocompatíveis/síntese química , Materiais Biocompatíveis/toxicidade , Biofilmes/efeitos dos fármacos , Cromatografia Líquida de Alta Pressão , Ciprofloxacina/química , Ciprofloxacina/farmacologia , Ciprofloxacina/toxicidade , Polpa Dentária/citologia , Polpa Dentária/efeitos dos fármacos , Difusão , Módulo de Elasticidade , Enterococcus faecalis/efeitos dos fármacos , Humanos , Teste de Materiais , Metronidazol/química , Metronidazol/farmacologia , Metronidazol/toxicidade , Microscopia Eletrônica de Varredura , Nanofibras/toxicidade , Polidioxanona/química , Polidioxanona/toxicidade , Porphyromonas gingivalis/efeitos dos fármacos , Espectroscopia de Infravermelho com Transformada de Fourier , Células-Tronco/efeitos dos fármacos , Estresse Mecânico , Propriedades de Superfície , Resistência à Tração , Fatores de Tempo
3.
J Am Assoc Lab Anim Sci ; 49(1): 64-8, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20122319

RESUMO

Invertebrates are increasing in their importance to both the public and private aquarium trade and play a vital role in biomedical research. Surgical techniques have become an important approach to obtaining data and maintaining good health in both of these areas. However, studies examining tissue reaction to suture material in invertebrates are lacking. The current study evaluated the gross and histologic reaction of Aplysia californica to 5 commonly used suture materials, including polydioxanone, black braided silk, polyglactin 910, monofilament nylon, and monofilament poliglecaprone. Histologic samples were graded on the amount of edema (score, 1 to 4), inflammation (1 to 4), and granuloma formation (1 to 4) present, and a final overall histology score (1 to 6) was assigned to each sample. Compared with untreated control tissue, all suture materials caused significantly increased tissue reaction, but the overall histology score did not differ among the suture materials. Silk was the only suture that did not have a significantly increased granuloma score when compared with the control. Although none of the suture materials evaluated seemed clearly superior for use in Aplysia, we recommend silk because of its less robust granuloma induction, which is favorable in a clinical and research setting.


Assuntos
Aplysia , Pele/patologia , Suturas/veterinária , Animais , Procedimentos Cirúrgicos Dermatológicos , Inflamação/epidemiologia , Inflamação/veterinária , Nylons/toxicidade , Polidioxanona/toxicidade , Poliglactina 910/toxicidade , Seda/toxicidade , Tela Subcutânea/patologia , Tela Subcutânea/cirurgia , Suturas/efeitos adversos
4.
J Am Assoc Lab Anim Sci ; 45(6): 22-6, 2006 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-17089987

RESUMO

Surgical harvest of Xenopus laevis oocytes for developmental research is a common procedure that requires closure of a 0.5- to 2.0-cm incision with suture material. Although such harvests are a frequent practice, little published information exists to provide guidance regarding the most appropriate suture material for wound closure in laboratory amphibians. To determine which suture material elicits the least response in amphibian skin, we used Xenopus laevis as a model to investigate the gross and histologic tissue reactions to 5 commonly used suture materials-3-0 silk, monofilament nylon, polydioxanone, polyglactin 910, and chromic gut. The skin reacted in 3 ways to suture material, showing edema, epidermal changes, and inflammation. Although the gross reactions to monofilament nylon, polydioxanone, and polyglactin 910 were clinically indistinguishable and were associated with lowest gross reaction scores, monofilament nylon elicited the least histologic reaction and therefore seems to be the most appropriate choice for use in amphibian skin.


Assuntos
Procedimentos Cirúrgicos Dermatológicos , Suturas , Xenopus laevis/cirurgia , Animais , Feminino , Inflamação/etiologia , Inflamação/patologia , Masculino , Nylons/toxicidade , Polidioxanona/toxicidade , Poliglactina 910/toxicidade , Seda/toxicidade , Pele/patologia
5.
J Biomed Mater Res ; 29(11): 1337-48, 1995 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8582902

RESUMO

The polymerization of desaminotyrosinetyrosylhexyl ester (DTH) with phosgene gives rise to poly(DTH carbonate), a new pseudopoly(amino acid). To evaluate the performance of this bioabsorbable material in orthopedic applications, the tissue responses elicited by compression-molded pins of poly(DTH carbonate) and clinically used polydioxanone pins (PDS; Orthosorb) were compared. The two types of pins were implanted in the paravertebral muscle and in the metaphyseal proximal tibia and distal femur of 10 White New Zealand Rabbits for 1, 2, 4, and 26 weeks. The tissue response was evaluated using histologic staining of soft- and hard-tissue sections, fluorescent bone marker of incorporation, and backscattered electron imaging. In soft tissue, both poly(DTH carbonate) and PDS elicited a mild inflammatory response resulting in encapsulation. During the disintegration phase, the PDS implants triggered a foreign body response involving the phagocytosis of polymeric debris by histiocytes and giant cells. No such response was observed for poly(DTH carbonate). In hard tissue, close bone apposition was observed throughout the 26-week test period for poly(DTH carbonate) implants. At the 26-week time point, the poly(DTH carbonate) implants exhibited surface erosion and were penetrated by new bone. In contrast, an intervening fibrous tissue layer was always present between the PDS pins and the bone. At 26 weeks, the PDS implants had partially resorbed and a foreign body response characterized by infiltration in several of the implantation sites. This study indicates that poly(DTH carbonate) and PDS exhibit fundamentally different interactions with hard tissue, and that poly(DTH carbonate) is a promising orthopedic implant material.


Assuntos
Materiais Biocompatíveis/toxicidade , Biopolímeros/toxicidade , Próteses e Implantes , Tirosina/análogos & derivados , Tirosina/toxicidade , Absorção , Animais , Materiais Biocompatíveis/química , Biopolímeros/química , Pinos Ortopédicos , Osso e Ossos/patologia , Osso e Ossos/fisiologia , Reação a Corpo Estranho/patologia , Masculino , Teste de Materiais , Peso Molecular , Músculo Esquelético/patologia , Músculo Esquelético/fisiologia , Polidioxanona/química , Polidioxanona/toxicidade , Coelhos , Tirosina/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...