RESUMO
Surface engineering of individual living cells is a promising field for cell-based applications. However, engineering individual cells with controllable thickness by chemical methods has been rarely studied. This article describes the development of a new cytocompatible chemical strategy to decorate individual living cells. The thicknesses of the crosslinked shells could be conveniently controlled by the irradiation time, visible light intensity, or monomer concentration. Moreover, the lag phase of the yeast cell division was extended and their stability against lysis was improved, which could also be tuned by controlling the shell thickness.
Assuntos
Engenharia Celular/métodos , Polietilenoglicóis/química , Polietilenoimina/química , Divisão Celular/efeitos dos fármacos , Parede Celular/metabolismo , Difusão , Glucana Endo-1,3-beta-D-Glucosidase/química , Glucana Endo-1,3-beta-D-Glucosidase/metabolismo , Luz , Complexos Multienzimáticos/química , Complexos Multienzimáticos/metabolismo , Peptídeo Hidrolases/química , Peptídeo Hidrolases/metabolismo , Polietilenoglicóis/síntese química , Polietilenoglicóis/efeitos da radiação , Polietilenoimina/síntese química , Polietilenoimina/efeitos da radiação , Polimerização , Saccharomyces cerevisiae/químicaRESUMO
Generation of supporting structures, which guide cell growth, is a challenging task in the field of tissue engineering. Cell guidance properties of a scaffold are important in the field of neuronal regeneration. Those guiding structures can provide guidance just by mechanical stimulus or by chemical stimuli like cell signaling molecules. For an enhanced guidance, chemical gradients are under investigation. With this study, we show that ultraviolet laser irradiation is a useful tool to activate polymer surfaces with a high temporal and spatial resolution. We demonstrated that poly(methyl methacrylate) (PMMA) and poly-ε-caprolactone (PCL) can be locally activated and functionalized with amine groups that can be used for immobilization of arginine-glycine-aspartic acid (RGD) peptide. The immobilized RGD was detected by neuronal B35 cells. By defined pulse accumulation functionalization density on the surface can be varied for the generation of gradients. We demonstrated that PMMA and PCL have different process windows for functionalization. Although PMMA has a very small process window for activation, PCL allows the generation of stepwise functionalization. The presented technology can help to develop assays for the analysis of cell migration and neuronal regeneration due to flexible patterning easily realized by changing the irradiation parameters.
