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1.
Anaerobe ; 72: 102469, 2021 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-34699978

RESUMO

OBJECTIVES: Blood culture bottles (BCBs) are commonly used for the diagnosis of infections associated with orthopedic devices. Although Cutibacterium acnes is an important pathogen in orthopedics, relatively little is known about its growth characteristics in BCBs. This prompted us to analyze the influence of bacterial genotype and clinical significance on time-to-detection (TTD) in BCBs. METHODS: We reviewed 59 cases of orthopedic device-related infections in which at least one intraoperative specimen yielded a pure C. acnes culture from anaerobic BCBs (BD Bactec Lytic/10 Anaerobic/F; Lytic-Ana) and/or solid media. A strain was considered infectant if the same genotype was present in two or more intraoperative samples. From these cases, we isolated a total of 72 unique C. acnes strains belonging to four multilocus sequence type clonal complexes (CCs): CC18, CC28, CC36 and CC53. Growth rate and TTD in Lytic-Ana BCB were studied under experimental conditions (inoculation of standard inoculum) and in clinical samples (inoculation of periprosthetic tissue samples). RESULTS: Median TTD values were shorter for CC53 compared to other CCs under experimental conditions (69 vs. 103 h; p < 0.001) and from clinical specimens (70 vs. 200 h; p = 0.02). Infectant strains had a shorter median TTD compared to contaminant strains in a clinical situation, while the difference was not observed under experimental conditions. CONCLUSIONS: The detection dynamics of C. acnes in Lytic-Ana BCBs were associated with genotype. Thus, TTD not only reflects the bacterial load in clinical samples, but may also reflect the intrinsic properties of the clonal complex of C. acnes.


Assuntos
Infecções por Bactérias Gram-Positivas/diagnóstico , Infecções por Bactérias Gram-Positivas/microbiologia , Propionibacterium acnes , Infecções Relacionadas à Prótese/diagnóstico , Infecções Relacionadas à Prótese/etiologia , Adulto , Idoso , Proteínas de Bactérias/genética , Técnicas de Tipagem Bacteriana , Hemocultura , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Tipagem de Sequências Multilocus , Procedimentos Ortopédicos/efeitos adversos , Propionibacterium acnes/classificação , Propionibacterium acnes/genética , Propionibacterium acnes/isolamento & purificação
2.
Exp Dermatol ; 30(3): 347-357, 2021 03.
Artigo em Inglês | MEDLINE | ID: mdl-33354825

RESUMO

Acne is an inflammatory skin disease of the pilosebaceous unit, involving four essential factors: hyperseborrhoea combined to a modification of sebum composition, colonization by Cutibacterium (C.) acnes, hyperkeratinization and secreted inflammation. Understanding and mimicking compromised skin is essential to further develop appropriate therapeutic solutions. This study aimed to develop new in vitro 3D models mimicking acneic skin, by combining two main factors involved in the physiopathology, namely, altered sebum composition and C. acnes invasion. Normal human keratinocytes were first used to generate reconstructed human epidermis (RHE) that were then left untreated (control) or treated topically with a combination of both peroxidized squalene and C. acnes cultures. Once validated, this model considered relevant to mimic acneic skin, was further improved by using different phylotypes of C. acnes strains specifically isolated from healthy and acneic patients. While both phylotypes IB and II did not significantly alter RHE, C. acnes IA1 strains induce major acneic skin hallmarks such as hyperkeratinization, secreted inflammation and altered barrier function. Interestingly, these results are obtained independently of the origin of IA1 phylotypes (acneic vs. healthy patient), thus suggesting a role of the ecosystem in controlling C. acnes virulence in healthy skin. In conclusion, by combining two major factors involved in the physiopathology of acne, we (1) succeeded to design in vitro 3D models mimicking this skin disorder and (2) highlighted how C. acnes phylotypes can have an impact on epidermal physiology. These relevant models will be suitable for the substantiation of therapeutic molecules dedicated to acne treatment.


Assuntos
Acne Vulgar/metabolismo , Acne Vulgar/microbiologia , Modelos Biológicos , Propionibacterium acnes , Sebo/metabolismo , Acne Vulgar/patologia , Citocinas/metabolismo , Epiderme , Humanos , Queratinócitos , Propionibacterium acnes/classificação , Fenômenos Fisiológicos da Pele , Esqualeno
3.
Anaerobe ; 62: 102169, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32058277

RESUMO

FMX101 4% minocycline foam (FMX101 4%) is a novel, topical minocycline formulation for treatment of acne vulgaris. We report that FMX101 4% had an MIC90 of 0.25 µg/ml and was ≥4-fold more active than comparator antimicrobials against a panel of 98 clinical Cutibacterium acnes isolates. The panel was diverse by clonal complex and sequence type, having 20 novel multi-locus sequence types including clonal complexes and sequence types associated with acne (CC1, CC3, and CC4; ST1 and ST3). Some isolates were phenotypically resistant to clindamycin (6.1%), erythromycin (14.3%), and tetracycline (2.0% intermediate resistance). Six isolates (6.4%) carried a mutation in the quinolone resistance-determining region of gyrA. With C. acnes, spontaneous resistance to FMX101 4% occurred at frequencies ranging from ≤5 × 10-9 to <1 × 10-8; mutations were identified in rpsJ, a gene encoding 30S ribosomal protein S10. No mutant exhibited a minocycline MIC above 0.5 µg/ml. No second-step mutation in previously isolated mutants or strains containing rpsJ ± 16S rRNA mutations was detected following minocycline challenge. Minocycline retained antibacterial activity against C. acnes over 15 multiple passages; thus, no selective growth advantage for minocycline-resistant mutants occurred under the experimental conditions. FMX101 4% has the potential to retain the favorable resistance profile of minocycline in diverse C. acnes isolates while providing the benefits of a topical formulation for treatment of acne vulgaris.


Assuntos
Antibacterianos/administração & dosagem , Minociclina/administração & dosagem , Propionibacterium acnes/efeitos dos fármacos , Farmacorresistência Bacteriana , Genótipo , Infecções por Bactérias Gram-Positivas/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Tipagem de Sequências Multilocus , Mutação , Propionibacterium acnes/classificação , Propionibacterium acnes/genética
4.
Anaerobe ; 61: 102133, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31813852

RESUMO

Cutibacterium acnes is a major etiologic agent of orthopaedic implant-associated infections (IAIs) and requires up to 14 days of incubation in an anaerobic atmosphere for growth detection. As blood culture (BC) systems are increasingly being used to monitor the growth of IAI specimens, we compared different BC media for growth detection of C. acnes. Non-duplicate C. acnes isolates (n = 99) obtained from sonicate-fluid cultures of orthopaedic IAIs from Slovenia (n = 54), conventional tissue samples of monomicrobial orthopaedic IAIs from France (n = 43) and two reference strains were inoculated to anaerobic BC bottles of two major BC systems and 3 conventional culture media types (thioglycolate broth, Schaedler and chocolate agar). Growth and time-to-detection (TTD) were recorded. Only Lytic (BACTEC) and SN (BacT/ALERT) bottles consistently detected growth of C. acnes within 14 days with 94% (n = 93) and 92% (n = 91) detection rates, respectively (p = 0.79). Lytic was superior to Plus BACTEC medium (p < 0.001), while SN was superior to all other BacT/ALERT media (p < 0.001). Mean TTD was 128 ± 43 h (61-336 h) for Lytic and 158 ± 65 h (77-336 h) for SN medium. Among the conventional media, 99% (n = 98) of the isolates grew on Schaedler agar, 96% (n = 95) in thioglycolate broth and 74% (n = 73) on chocolate agar. Inconsistent growth of C. acnes in different BC media can critically influence the detection of this major IAI pathogen. Only Lytic (BACTEC) and SN (BacT/ALERT) BC media types were consistently able to detect C. acnes within 14 days of incubation. However, visible growth was observed faster in thioglycolate broth and Schaedler agar media.


Assuntos
Infecções por Bactérias Gram-Positivas/diagnóstico , Infecções por Bactérias Gram-Positivas/microbiologia , Procedimentos Ortopédicos/efeitos adversos , Propionibacterium acnes , Infecções Relacionadas à Prótese/diagnóstico , Infecções Relacionadas à Prótese/microbiologia , Técnicas de Tipagem Bacteriana , Hemocultura , Humanos , Propionibacterium acnes/classificação , Propionibacterium acnes/isolamento & purificação
5.
Clin Infect Dis ; 68(11): 1942-1945, 2019 05 17.
Artigo em Inglês | MEDLINE | ID: mdl-31098616

RESUMO

Cutibacterium acnes orthopedic device-related infections (ODRIs) range from obvious infections to solely culture-based diagnoses. Multilocus sequence typing of multiple isolates from the same procedure revealed that most cases with normal C-reactive protein levels that were classified as C. acnes ODRI would be considered contaminations when accounting for genotypic data.


Assuntos
Contaminação de Equipamentos , Infecções por Bactérias Gram-Positivas/diagnóstico , Infecções por Bactérias Gram-Positivas/microbiologia , Tipagem de Sequências Multilocus , Propionibacterium acnes/classificação , Adulto , Idoso , Técnicas de Tipagem Bacteriana , Feminino , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Propionibacterium acnes/isolamento & purificação
6.
Anaerobe ; 59: 54-60, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31075312

RESUMO

Prosthetic joint infections (PJIs) are rare but feared complications following joint replacement surgery. Cutibacterium acnes is a skin commensal that is best known for its role in acne vulgaris but can also cause invasive infections such as PJIs. Some phylotypes might be associated with specific diseases, and recently, a plasmid was detected that might harbour important virulence genes. In this study, we characterized C. acnes isolates from 63 patients with PJIs (n = 140 isolates) and from the skin of 56 healthy individuals (n = 56 isolates), using molecular methods to determine the phylotype and investigate the presence of the plasmid. Single-locus sequence typing and a polymerase chain reaction designed to detect the plasmid were performed on all 196 isolates. No statistically significant differences in sequence types were seen between the two study groups indicating that the C. acnes that causes PJIs originates from the patients own normal skin microbiota. Of the 27 patients with multiple tissue samples, 19 displayed the same sequence types among all their samples. Single-locus sequence typing identified different genotypes among consecutive C. acnes isolates from four patients with recurrent infections. The plasmid was found among 17 isolates distributed in both groups, indicating that it might not be a marker for virulence regarding PJIs. Patients presenting multiple sequence types in tissue samples may represent contamination or a true polyclonal infection due to C. acnes.


Assuntos
Artrite/microbiologia , Portador Sadio/microbiologia , Genótipo , Infecções por Bactérias Gram-Positivas/microbiologia , Propionibacterium acnes/classificação , Propionibacterium acnes/genética , Infecções Relacionadas à Prótese/microbiologia , Artrite/epidemiologia , Humanos , Epidemiologia Molecular , Tipagem Molecular , Plasmídeos/análise , Reação em Cadeia da Polimerase , Propionibacterium acnes/isolamento & purificação , Infecções Relacionadas à Prótese/epidemiologia , Análise de Sequência de DNA
8.
Int J Syst Evol Microbiol ; 69(4): 1087-1092, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30762517

RESUMO

In 2016, division of the genus Propionibacterium into four distinct genera was proposed. As a consequence, the species Propionibacterium acnes was transferred to Cutibacterium gen. nov. as Cutibacterium acnes comb. nov. The three recently proposed subspecies of P. acnes were not, however, accommodated in this proposal. Following a very recent validation of a new combination for C. acnessubsp.defendens and an automatically created C. acnessubsp.acnes, we now propose the new combination, C. acnessubsp. elongatum comb. nov. The type strain of Cutibacterium acnes subsp. elongatum is JCM 18919T (=NCTC 13655T). On the basis of further genomic and phenotypic (haemolysis and MALDI-TOF mass spectrometry) analyses of these subspecies, we also provide emended descriptions of the genus Cutibacterium Scholz and Kilian 2016, C. acnessubsp.acnes (Gilchrist 1900) Nouioui et al. 2018, and C. acnessubsp.defendens (McDowell et al. 2016) Nouioui et al. 2018.


Assuntos
Filogenia , Propionibacterium acnes/classificação , Pele/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Humanos , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA
9.
Ann Dermatol Venereol ; 146(1): 26-30, 2019 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-30558958

RESUMO

Since its discovery, Propionibacterium acnes has undergone various name changes, and has been known since 2016, as Cutibacterium acnes. Herein we set out the history and rational of these taxonomic changes together with a description of a new genus, Cutibacterium, which includes five species within the cutaneous ecosystem. Modern microbiological techniques allow finer distinction between species and subspecies while also enabling the identification of separate subtypes within the population of Cutibacterium acnes. Phylogeny and molecular typing techniques thus provide a better understanding of the subtypes involved in certain inflammatory skin diseases, including acne, folliculitis and progressive macular hypomelanosis.


Assuntos
Acne Vulgar/microbiologia , Propionibacterium acnes/classificação , Propionibacterium acnes/genética , Humanos , Tipagem Molecular , Filogenia , RNA Ribossômico 16S , Análise de Sequência de RNA
10.
J Eur Acad Dermatol Venereol ; 32 Suppl 2: 15-23, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29894577

RESUMO

OBJECTIVE: Our main objective was to compare Cutibacterium acnes (C. acnes) skin colonisation in patients with mild to moderate acne versus healthy controls and secondly, to evaluate a Myrtacine® -based cream on C. acnes total population and antibioresistant Cutibacteria in patients with acne. METHODS: In 60 acne patients (Global Acne Severity Scale, GEA grades 2-3), of mean age 20 [15-30] years and in 24 age- and sex- matched healthy controls, forehead strips samplings were performed for microbiological analysis of comedones by colony forming unit (CFU) counts of global C. acnes and erythromycin (EryR) or clindamycin-resistant (ClnR) populations of Cutibacterium and determination of phylotypes by MALTI-TOF. Clinical evaluations of acne patients (GEA, lesion count, porphyrin fluorescence) were performed at baseline and after 56 days of twice-daily application of a Myrtacine® -based cream. RESULTS: We first showed (i) high and similar levels of C. acnes colonisation in superficial pilosebaceous follicles and detection of EryR and ClnR strains in both acne and control groups; (ii) different repartition of phylotypes in acne patients versus healthy control, with a predominance of phylotype IA in acne patients and a link between phylotype IA and erythromycin resistance. Besides, after treatment with the Myrtacine® -based cream in acne patients, there was no change in C. acnes total load, but a significant decrease of EryR Cutibacteria, reduced porphyrin production by C. acnes, a decrease in acne severity (GEA), associated with reduced retentional and inflammatory lesions. CONCLUSION: Cutibacterium acnes colonisation was not significantly different in acne versus control groups. Phylotype IA was predominant in acne patient and in EryR C. acnes. A Myrtacine® -based cream significantly reduced the level of EryR Cutibacteria in vivo and improved acne lesions.


Assuntos
Acne Vulgar/tratamento farmacológico , Acne Vulgar/microbiologia , Extratos Vegetais/uso terapêutico , Propionibacterium acnes/isolamento & purificação , Adolescente , Adulto , Carga Bacteriana , Estudos de Casos e Controles , Feminino , Humanos , Masculino , Propionibacterium acnes/classificação , Índice de Gravidade de Doença , Adulto Jovem
11.
J Eur Acad Dermatol Venereol ; 32 Suppl 2: 5-14, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29894579

RESUMO

While the commensal bacterium Propionibacterium acnes (P. acnes) is involved in the maintenance of a healthy skin, it can also act as an opportunistic pathogen in acne vulgaris. The latest findings on P. acnes shed light on the critical role of a tight equilibrium between members of its phylotypes and within the skin microbiota in the development of this skin disease. Indeed, contrary to what was previously thought, proliferation of P. acnes is not the trigger of acne as patients with acne do not harbour more P. acnes in follicles than normal individuals. Instead, the loss of the skin microbial diversity together with the activation of the innate immunity might lead to this chronic inflammatory condition. This review provides results of the most recent biochemical and genomic investigations that led to the new taxonomic classification of P. acnes renamed Cutibacterium acnes (C. acnes), and to the better characterisation of its phylogenetic cluster groups. Moreover, the latest data on the role of C. acnes and its different phylotypes in acne are presented, providing an overview of the factors that could participate in the virulence and in the antimicrobial resistance of acne-associated strains. Overall, this emerging key information offers new perspectives in the treatment of acne, with future innovative strategies focusing on C. acnes biofilms and/or on its acne-associated phylotypes.


Assuntos
Acne Vulgar/microbiologia , Propionibacterium acnes/classificação , Humanos , Propionibacterium acnes/fisiologia
13.
Acta Derm Venereol ; 98(2): 262-267, 2018 02 07.
Artigo em Inglês | MEDLINE | ID: mdl-29136261

RESUMO

Propionibacterium acnes, a major member of normal skin microbiota, is subdivided into 6 phylotypes: IA1, IA2, IB, IC, II and III. This study investigated P. acnes subgroups on the face and back in patients with severe acne and in healthy controls. In 71.4% of patients with severe acne, P. acnes phylotypes were identical on the face and back, whereas this was the case in only 45.5% of healthy controls. The healthy group carried phylotypes IA1 (39.1%) and II (43.4%), whereas the acne group carried a high predominance of IA1 (84.4%), especially on the back (95.6%). In addition, the single-locus sequence typing (SLST) method revealed A1 to be the predominant type on the back of patients with acne, compared with a wide diversity in the healthy group. We report here that severity of acne on the back is associated with loss of diversity of P. acnes phylotype, with a major predominance of phylotype IA1. The change in balance of cutaneous P. acnes subgroups might be an inducing factor in the activation of P. acnes, which could trigger inflammation.


Assuntos
Acne Vulgar/microbiologia , Filogenia , Propionibacterium acnes/classificação , Pele/microbiologia , Acne Vulgar/diagnóstico , Adolescente , Adulto , Dorso , Estudos de Casos e Controles , Face , Feminino , Genótipo , Humanos , Masculino , Propionibacterium acnes/genética , Propionibacterium acnes/isolamento & purificação , Índice de Gravidade de Doença , Adulto Jovem
14.
Anaerobe ; 48: 172-176, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28859990

RESUMO

Cutibacterium (formerly Propionibacterium) acnes is involved in chronic/low-grade pathologies such as sarcoidosis or prosthetic joint infection (PJI). In these diseases, granulomatous structures are frequently observed. In this study, we induced a physiological granulomatous reaction in response to different well-characterized clinical C. acnes isolates in order to investigate the cellular process during granuloma formation. Three C. acnes isolates selected according to their origin (PJI, sarcoidosis and acne) were typed by MLST. All C. acnes isolates generated granulomatous structures in our experimental conditions. The bacterial burden was better controlled by granulomas induced by the sarcoidosis C. acnes isolate. The PJI C. acnes isolate, belonging to CC36, promoted the recruitment of CD8+ lymphocytes inside the granuloma. In contrast, the acne and sarcoidosis C. acnes isolates, belonging to phylotypes IA1/CC18 and IA2/CC28, respectively, generated a higher number of granulomas and promoted the recruitment of CD4+ lymphocytes inside the granuloma. Our results provide new evidence supporting the role of C. acnes in the development of sarcoidosis and new explanations concerning the mechanisms underlying PJI due to C. acnes.


Assuntos
Infecções por Bactérias Gram-Positivas/complicações , Infecções por Bactérias Gram-Positivas/imunologia , Granuloma/etiologia , Imunidade , Propionibacterium acnes/imunologia , Suscetibilidade a Doenças , Humanos , Leucócitos Mononucleares/imunologia , Leucócitos Mononucleares/microbiologia , Tipagem de Sequências Multilocus , Propionibacterium acnes/classificação , Propionibacterium acnes/genética
15.
Eur J Clin Microbiol Infect Dis ; 36(12): 2483-2489, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28842758

RESUMO

The study aimed to retrospectively assess if strain typing of Propionibacterium acnes could help to distinguish between infection and contamination in isolates recovered from the central nervous system (CNS) and prosthetic joints (PJs). This was a retrospective cohort of all Propionibacterium species isolates from the Barnes-Jewish Hospital (St Louis, MO, USA) clinical microbiology laboratory from 2011 to 2014. Available frozen isolates were recovered, and strain type (IA-1, IA-2, IB, II, III, or nontypeable class A or B) was determined via polymerase chain reaction (PCR)-based methods. For CNS isolates, P. acnes was considered pathogenic if treating physicians administered ≥7 days of directed antibiotic therapy against P. acnes. During the study period, Propionibacterium species was isolated from clinical cultures 411 times. 152 isolates were available for analysis. Of the 152 isolates, 140 were confirmed to be P. acnes, 61 of which were from the CNS (45 contaminants, 16 infections). Strain type IA-1 was more common (50.0%, 8 out of 16) among CNS infections than among contaminants (22.2%, 10 out of 45). For PJ isolates 61.3% (19 out of 31) met the criteria for infection. The predominant strain type for CNS infection was IA-1 and for PJ isolates, IB. Strain type IA-1 was isolated more often in patients with CNS infections, which may indicate a predilection of this strain type to cause CNS infection. Future research should prospectively evaluate strain typing as a means of assisting in the diagnosis of CNS infections and confirm our findings.


Assuntos
Artrite Infecciosa/microbiologia , Infecções Bacterianas do Sistema Nervoso Central/microbiologia , Propionibacterium acnes/classificação , Infecções Relacionadas à Prótese/microbiologia , Adulto , Artrite Infecciosa/diagnóstico , Infecções Bacterianas do Sistema Nervoso Central/diagnóstico , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Tipagem de Sequências Multilocus , Propionibacterium acnes/genética , Infecções Relacionadas à Prótese/diagnóstico , Estudos Retrospectivos , Adulto Jovem
16.
J Microbiol Methods ; 141: 17-24, 2017 10.
Artigo em Inglês | MEDLINE | ID: mdl-28732695

RESUMO

Propionibacterium acnes is a key factor in the pathogenesis of acne vulgaris, although currently it is also being associated with medical-device infections. The aim of this work was to validate a safe and quick identification and typing of 24 clinical isolates of Propionibacterium acnes, applying a range of biochemical as well as genetic methods, and investigating the pathogenic potential to associate the different types with human health. RAPD-PCRs revealed the existence of two discernible clusters in correspondence with the phylogroups I and II, according to the PAtig gene polymorphism, leading them to be assigned as P. acnes subsp. acnes subsp. nov. Biotyping according to the pattern of sugar fermentation evidenced that all the isolates from acne and the majority from opportunistic infections fit the biotype I-B3. Consistent with the multiplex touchdown analysis, nearly all the isolates included in this biotype belonged to the subgroups IA1 (the exception being four strains classified as IB). The remaining ones were assigned to phylogroup II, considered to be part of the normal cutaneous microbiota. The susceptibility to three antibiotics was also investigated to explore the relations with the virulence, although no clear trend was identified.


Assuntos
Infecções por Bactérias Gram-Positivas/microbiologia , Tipagem Molecular/métodos , Propionibacterium acnes/classificação , Propionibacterium acnes/isolamento & purificação , Técnica de Amplificação ao Acaso de DNA Polimórfico/métodos , Antibacterianos/farmacologia , DNA Bacteriano/genética , Genótipo , Humanos , Filogenia , Propionibacterium acnes/efeitos dos fármacos , Propionibacterium acnes/genética , Pele/microbiologia
17.
Anaerobe ; 47: 57-63, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28434779

RESUMO

BACKGROUND: Cutibacterium (Propionibacterium) acnes is assumed to play an important role in the pathogenesis of acne. OBJECTIVES: To examine if clones with distinct virulence properties are associated with acne. METHODS: Multiple C. acnes isolates from follicles and surface skin of patients with moderate to severe acne and healthy controls were characterized by multilocus sequence typing. To determine if CC18 isolates from acne patients differ from those of controls in the possession of virulence genes or lack of genes conducive to a harmonious coexistence the full genomes of dominating CC18 follicular clones from six patients and five controls were sequenced. RESULTS: Individuals carried one to ten clones simultaneously. The dominating C. acnes clones in follicles from acne patients were exclusively from the phylogenetic clade I-1a and all belonged to clonal complex CC18 with the exception of one patient dominated by the worldwide-disseminated and often antibiotic resistant clone ST3. The clonal composition of healthy follicles showed a more heterogeneous pattern with follicles dominated by clones representing the phylogenetic clades I-1a, I-1b, I-2 and II. Comparison of follicular CC18 gene contents, allelic versions of putative virulence genes and their promoter regions, and 54 variable-length intragenic and inter-genic homopolymeric tracts showed extensive conservation and no difference associated with the clinical origin of isolates. CONCLUSIONS: The study supports that C. acnes strains from clonal complex CC18 and the often antibiotic resistant clone ST3 are associated with acne and suggests that susceptibility of the host rather than differences within these clones may determine the clinical outcome of colonization.


Assuntos
Acne Vulgar/microbiologia , Variação Genética , Infecções por Bactérias Gram-Positivas/microbiologia , Voluntários Saudáveis , Propionibacterium acnes/classificação , Propionibacterium acnes/isolamento & purificação , Adolescente , Adulto , Feminino , Genótipo , Humanos , Masculino , Tipagem de Sequências Multilocus , Propionibacterium acnes/genética , Pele/microbiologia , Fatores de Virulência/análise , Fatores de Virulência/genética , Sequenciamento Completo do Genoma , Adulto Jovem
18.
Anaerobe ; 47: 73-78, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28454760

RESUMO

The recognition of the pathogenicity of Cutibacterium acnes in implant-associated infection is not always obvious. In this paper, we aimed to distinguish pathogenic and non-pathogenic C. acnes isolates. To reach this goal, we investigated the clonal complex (CC) of a large collection of C. acnes clinical isolates through Multi-Locus Sequence Typing (MLST), we established a Caenorhabditis elegans model to assess C. acnes virulence and we investigated the presence of virulence factors in our collection. Ours results showed that CC36 and CC53 C. acnes isolates were more frequently observed in prosthetic joint infections (PJI) than CC18 and CC28 C. acnes isolates (p = 0.021). The C. elegans model developed here showed two distinct virulence groups of C. acnes (p < 0.05). These groups were not correlated to CC or clinical origin. Whole genome sequencing allowed us to identify a putative gene linked to low virulent strains. In conclusion, MLST remains a good method to screen pathogenic C. acnes isolates according to their clinical context but mechanisms of C. acnes virulence need to be assess thought transcriptomic analysis to investigate regulatory process.


Assuntos
Infecções por Bactérias Gram-Positivas/microbiologia , Propionibacterium acnes/fisiologia , Propionibacterium acnes/patogenicidade , Infecções Relacionadas à Prótese/microbiologia , Tropismo Viral , Animais , Caenorhabditis elegans/microbiologia , Caenorhabditis elegans/fisiologia , Modelos Animais de Doenças , Humanos , Tipagem de Sequências Multilocus , Propionibacterium acnes/classificação , Propionibacterium acnes/genética , Análise de Sobrevida , Virulência , Fatores de Virulência/análise , Fatores de Virulência/genética , Sequenciamento Completo do Genoma
20.
Respir Res ; 18(1): 28, 2017 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-28143482

RESUMO

OBJECTIVE: This study aims to use high throughput 16SrRNA gene sequencing to examine the bacterial profile of lymph node biopsy samples of patients with sarcoidosis and to further verify the association between Propionibacterium acnes (P. acnes) and sarcoidosis. METHODS: A total of 36 mediastinal lymph node biopsy specimens were collected from 17 cases of sarcoidosis, 8 tuberculosis (TB group), and 11 non-infectious lung diseases (control group). The V4 region of the bacterial 16SrRNA gene in the specimens was amplified and sequenced using the high throughput sequencing platform MiSeq, and bacterial profile was established. The data analysis software QIIME and Metastats were used to compare bacterial relative abundance in the three patient groups. RESULTS: Overall, 545 genera were identified; 38 showed significantly lower and 29 had significantly higher relative abundance in the sarcoidosis group than in the TB and control groups (P < 0.01). P. acnes 16SrRNA was exclusively found in all the 17 samples of the sarcoidosis group, whereas was not detected in the TB and control groups. The relative abundance of P. acnes in the sarcoidosis group (0.16% ± 0. 11%) was significantly higher than that in the TB (Metastats analysis: P = 0.0010, q = 0.0044) and control groups (Metastats analysis: P = 0.0010, q = 0.0038). The relative abundance of P. granulosum was only 0.0022% ± 0. 0044% in the sarcoidosis group. P. granulosum 16SrRNA was not detected in the other two groups. CONCLUSION: High throughput 16SrRNA gene sequencing appears to be a useful tool to investigate the bacterial profile of sarcoidosis specimens. The results suggest that P. acnes may be involved in sarcoidosis development.


Assuntos
Infecções por Bactérias Gram-Positivas/microbiologia , Linfonodos/microbiologia , Propionibacterium acnes/genética , Propionibacterium acnes/isolamento & purificação , RNA Ribossômico 16S/genética , Sarcoidose Pulmonar/microbiologia , Feminino , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Sequenciamento de Nucleotídeos em Larga Escala/estatística & dados numéricos , Humanos , Masculino , Pessoa de Meia-Idade , Propionibacterium acnes/classificação , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise de Sequência de RNA/métodos , Análise de Sequência de RNA/estatística & dados numéricos , Estatística como Assunto
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