RESUMO
Extracting venom from small species is usually challenging. We describe here an affordable and versatile electrical venom extractor based on the Arduino(®) Mega 2560 Board, which is designed to extract venom from arthropods and other small animals. The device includes fine tuning of stimulation time and voltage. It was used to collect venom without apparent deleterious effects, and characterized for the first time the venom of Zoropsis spinimana, a common spider in French Mediterranean regions.
Assuntos
Manejo de Espécimes/instrumentação , Venenos de Aranha/isolamento & purificação , Aranhas/fisiologia , Animais , Proteínas de Artrópodes/análise , Proteínas de Artrópodes/química , Proteínas de Artrópodes/economia , Proteínas de Artrópodes/isolamento & purificação , Venenos de Artrópodes/química , Venenos de Artrópodes/economia , Venenos de Artrópodes/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Custos e Análise de Custo , Equipamentos e Provisões Elétricas/economia , Desenho de Equipamento , França , Teste de Materiais , Região do Mediterrâneo , Peso Molecular , Restrição Física/instrumentação , Manejo de Espécimes/economia , Espectrometria de Massas por Ionização por Electrospray , Venenos de Aranha/química , Venenos de Aranha/economia , Aranhas/crescimento & desenvolvimentoRESUMO
Recently, much attention has been given to improving the antioxidant activity of protein hydrolysates via the Maillard reaction, but little is known about the cellular antioxidant activity of Maillard reaction products (MRPs) from protein hydrolysates. We first investigated chemical characterization and the cellular antioxidant activity of MRPs in a shrimp (Litopenaeus vannamei) by-product protein hydrolysate (SBH)-glucose system at 110 °C for up to 10 h of heating. Solutions of SBH and glucose were also heated alone as controls. The Maillard reaction greatly resulted in the increase of hydroxymethylfurfural (HMF) and browning intensity, high molecular weight fraction, and reduction of the total amino acid in SBH with the heating time, which correlated well with the free radical scavenging activity of MRPs. MRPs had stronger inhibiting effects on oxidative stress of human HepG2 cells than the original SBH, and its cellular antioxidant activity strongly correlated with free radical scavenging activity, but less affected by the browning intensity and HMF level. The caramelization of glucose partially affected the HMF level and free radical scavenging activity of MRPs, but it was not related to the cellular antioxidant activity. The cellular antioxidant activity of MRPs for 5 h of heating time appeared to reach a maximum level, which was mainly due to carbonyl ammonia condensation reaction. In conclusion, the Maillard reaction is a potential method to increase the cellular antioxidant activity of a shrimp by-product protein hydrolysate, but the higher HMF levels and the lower amino acid content in MRPs should also be considered.
