RESUMO
OBJECTIVE: To study seed quality test, and establish seed quality classification standard of Prunella vulgaris. METHOD: The seed quality and planting quality of P. vulgaris from different population were measured and these indexes were analyzed by K-clustering. RESULT AND CONCLUSION: The seed purity, 1000 seeds weight, seed moisture content, seed viability of the seed of P. vulgaris from different population were measured, and the seed quality classification standard of P. vulgaris.
Assuntos
Prunella/classificação , Sementes/classificação , Análise por Conglomerados , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/classificação , Medicamentos de Ervas Chinesas/normas , Prunella/química , Prunella/fisiologia , Controle de Qualidade , Sementes/química , Sementes/fisiologia , Água/análiseRESUMO
OBJECTIVE: To explore the variety of the genetic polymorphism of eight Prunella germplasm resources by AFLP analysis. METHOD: The amplified fragment length polymorphism (AFLP) tags were applied to screen out 32 selective amplification primer pairs, the amplified bands as original matrix were analyzed with NTSYS-PC software for the similarity between the Prunella germplasm and the construction of genetic phylogenetic tree. RESULT: SDS extraction of genomic Prunella DNA showed a good quality, could meet the requirements of AFLP analysis. From 32 selective amplification primer pairs, 10 pairs with strong polymorphism, better band and higher resolution were used for the construction of the AFLP Prunella fingerprint, all eight Prunella germplasms were separated, they were divided into 3 categories. CONCLUSION: Prunella germplasm resources are rich in genetic diversity, certain morphological characteristics and differences are associate with genotype.
