Integrated Bayesian species delimitation and morphological diagnostics of chorioptic mange mites (Acariformes: Psoroptidae: Chorioptes).

The external morphology of adult and immature stages of mange mites of the genus Chorioptes was investigated with the aid of light and scanning electron microscopy. A molecular phylogeny of this genus was inferred based on six genes (18S, 28S rDNA, EF1-α, SRP54, HSP70, and CO1). The validity of four species (Ch. bovis, Ch. panda, Ch. texanus, and Ch. sweatmani sp. nov. described from the moose from Sweden, Finland, and Russia) was confirmed based on morphology and a Bayesian species delimitation analysis incorporating both gene tree uncertainties and incomplete lineage sorting via the coalescent process model in BPP. Sequence data for Ch. crewei and Ch. mydaus was not available but their morphology strongly suggests their validity. The six valid Chorioptes species are diagnosed using type and non-type specimens, and a key to species is provided. Ch. sweatmani differs from closely related Ch. texanus by the following features: in males, the body length, including the gnathosoma, is 380-405 µm (vs. 220-295 in Ch. texanus), the idiosoma is 3-4 times longer than setae cp (vs. 1.3-1.6 times longer), legs III are approximately three times longer than setae sRIII (vs. 1.8-2 times longer), the apical spur of tarsus III is curved (vs. straight), a spur near seta fIII base is not developed (vs. small but distinct); in females, setae h2 are 1.4-1.5 times shorter than legs IV (vs. about two times longer). Hosts and distribution records of Chorioptes species are summarized.

Chorioptes mites: re-evaluation of species integrity.

Chorioptes mites (Acari: Psoroptidae) are non-burrowing agents of mange which parasitize a wide range of domesticated and wild ungulates. Considerable historical confusion has surrounded the description and naming of the various species of Chorioptes. Here, the opisthosomal setae of male adult Chorioptes mites from a range of host species and geographic locations were subjected to detailed analysis. Discriminant analysis suggests the existence of three clear morphotypes. The first, Chorioptes bovis, is characterized by a very long seta 1 (ae) and short spatulate setae 2 (l4 and d5), whereas, in the second, Chorioptes texanus, setae 2 (l4 and d5) are longer and narrower than in C. bovis, but most of the other setae are shorter; this is particularly true for seta 1 (ae). A third morphotype is characterized by a seta 1 (ae) that is shorter than in C. bovis but longer than in C. texanus, long setae 2 (l4 and d5) and a long seta 6 (l5). The data are compared with previously published measurements. Although this analysis supports the validity of C. bovis and C. texanus as morphologically distinct, it also supports the existence of a proposed third species of Chorioptes, as described by Hestvik et al. in 2007, and suggests that the mites described by Sweatman in his classic 1958 description of C. texanus, which were obtained from the ears of semi-domesticated reindeer, are likely to have been specimens of this as yet unnamed species. The results show that there is a high degree of phenotypic plasticity in setal lengths. It is concluded that a statistical difference in the absolute measurement of any one seta is not a reliable species indicator; the relative sizes of groups of setae would appear to be more taxonomically informative and should be used when identifying Chorioptes morphologically.

Ultrastructural morphology of the male and female genital tracts of Psoroptes spp. (Acari: Astigmata: Psoroptidae).

The structure of the male and female genital systems of the astigmatid mite Psoroptes ovis (Hering) is described. The male genital system is composed of a paired testis, fused at its proximal part, two vasa deferentia, an ejaculatory duct, into which a single accessory gland opens, and a copulatory organ. The testis is characterized by a peripheric syncytial cell surrounding spermatogonia, spermatocytes, spermatids and spermatozoa which are distributed regularly in the gonad according to the sequence of spermatogenesis. The female genital system consists of a copulatory pore (the bursa copulatrix), a seminal receptacle, paired ovaries and oviducts, a glandular uterus and an ovipositor which leads to the oviporus. Ovaries are composed of somatic cells, germ cells and a central cell, with a multilobular nucleus, connected to oocytes by a stalk. Similarities with other astigmatic mites belonging to Psoroptidia and Acaridia are also discussed.

Structure and function of the gnathosoma of the mange mite, Psoroptes ovis.

Mites of the genus Psoroptes (Acari: Psoroptidae) are obligate, non-burrowing, astigmatid ectoparasites of mammals. A detailed understanding of the morphology of the gnathosoma is an important step towards elucidation of the feeding behaviour of this mite and, hence, the pathology of psoroptic mange. Scanning and transmission electron microscopy were used to examine Psoroptes ovis (Hering) (syn. P. cuniculi). The gnathosoma is composed of an infracapitulum (hypostome), which forms the floor of an extended U-shaped pre-oral trough, bounded by laterally compressed palps and enclosing paired chelicerae. Distally, each palp terminates in a flap-like process and four tapered terminal sensillae. The floor of the infracapitulum is interrupted along its length by a dorsally projecting ridge. At the distal end of the infracapitulum are paired, grooved pseudorutellae and, between them, paired, ungrooved lateral lips. Between the lateral lips is a grooved, elongate labium. Medially, a pair of finger-like projections emanates from the paraxial walls of the palps and project anteriorly. Each chelicera is made up of fixed and mobile digits, with a cheliceral membranous fold articulating the two. The distal ends of both digits are chelated. For much of its length, the mobile cheliceral digit appears to lie within a fold formed by the infracapitular ridge, thereby creating a central channel between the chelicerae. It is suggested that this arrangement of elements may facilitate a two-way flow of liquid, where saliva flows down a central salivary canal and spills out onto the host's skin over the labium at the point where the chelated tips of the chelicerae meet and abrade the skin. Liquid food may move along the grooves of the pseudorutellae and then be sucked up a food canal formed by the paraxial walls of the palps and the outer wall of the chelicerae.