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1.
Mol Biol Rep ; 51(1): 738, 2024 Jun 14.
Artigo em Inglês | MEDLINE | ID: mdl-38874633

RESUMO

BACKGROUND: Interspecific hybrids of rohu (Labeo rohita) and catla (Labeo catla) are common, especially in India due to constrained breeding. These hybrids must segregate from their wild parents as part of conservational strategies. This study intended to screen the hybrids from wild rohu and catla parents using both morphometric and molecular approaches. METHODS & RESULTS: The carp samples were collected from Jharkhand and West Bengal, India. The correlation and regression analysis of morphometric features are considered superficial but could be protracted statistically by clustering analysis and further consolidated by nucleotide variations of one mitochondrial and one nuclear gene to differentiate hybrids from their parents. Out of 21 morphometric features, 6 were used for clustering analysis that exhibited discrete separation among rohu, catla, and their hybrids when the data points were plotted in a low-dimensional 2-D plane using the first 2 principal components. Out of 40 selected single nucleotide polymorphism (SNP) positions of the COX1 gene, hybrid showed 100% similarity with catla. Concerning SNP similarity of the 18S rRNA nuclear gene, the hybrid showed 100% similarity with rohu but not with catla; exhibiting its probable parental inheritance. CONCLUSIONS: Along with morphometric analysis, the SNP comparison study together points towards strong evidence of interspecific hybridization between rohu and catla, as these hybrids share both morphological and molecular differences with either parent. However, this study will help screen the hybrids from their wild parents, as a strategy for conservational management.


Assuntos
Carpas , Hibridização Genética , Polimorfismo de Nucleotídeo Único , Animais , Carpas/genética , Carpas/anatomia & histologia , Hibridização Genética/genética , Polimorfismo de Nucleotídeo Único/genética , Índia , RNA Ribossômico 18S/genética , Filogenia , Cyprinidae/genética , Cyprinidae/anatomia & histologia , Quimera/genética , Análise por Conglomerados
2.
Mol Plant Pathol ; 25(6): e13487, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38877765

RESUMO

We had previously reported that a plum pox virus (PPV)-based chimera that had its P1-HCPro bi-cistron replaced by a modified one from potato virus Y (PVY) increased its virulence in some Nicotiana benthamiana plants, after mechanical passages. This correlated with the natural acquisition of amino acid substitutions in several proteins, including in HCPro at either position 352 (Ile→Thr) or 454 (Leu→Arg), or of mutations in non-coding regions. Thr in position 352 is not found among natural potyviruses, while Arg in 454 is a reversion to the native PVY HCPro amino acid. We show here that both mutations separately contributed to the increased virulence observed in the passaged chimeras that acquired them, and that Thr in position 352 is no intragenic suppressor to a Leu in position 454, because their combined effects were cumulative. We demonstrate that Arg in position 454 improved HCPro autocatalytic cleavage, while Thr in position 352 increased its accumulation and the silencing suppression of a reporter in agropatch assays. We assessed infection by four cloned chimera variants expressing HCPro with none of the two substitutions, one of them or both, in wild-type versus DCL2/4-silenced transgenic plants. We found that during infection, the transgenic context of altered small RNAs affected the accumulation of the four HCPro variants differently and hence, also infection virulence.


Assuntos
Substituição de Aminoácidos , Nicotiana , Potyvirus , Proteínas Virais , Virulência/genética , Nicotiana/virologia , Potyvirus/patogenicidade , Potyvirus/genética , Proteínas Virais/metabolismo , Proteínas Virais/genética , Doenças das Plantas/virologia , Quimera , Vírus Eruptivo da Ameixa/patogenicidade , Vírus Eruptivo da Ameixa/genética , Cisteína Endopeptidases/metabolismo , Cisteína Endopeptidases/genética , Mutação/genética
3.
Stem Cell Reports ; 19(6): 877-889, 2024 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-38729156

RESUMO

Liver disease is a major global health challenge. There is a shortage of liver donors worldwide, and hepatocyte transplantation (HT) may be an effective treatment to overcome this problem. However, the present approaches for generation of hepatocytes are associated with challenges, and interspecies chimera-derived hepatocytes produced by interspecies blastocyst complementation (IBC) may be promising donor hepatocytes because of their more comprehensive hepatic functions. In this study, we isolated mouse hepatocytes from mouse-rat chimeric livers using IBC and found that interspecies chimera-derived hepatocytes exhibited mature hepatic functions in terms of lipid accumulation, glycogen storage, and urea synthesis. Meanwhile, they were more similar to endogenous hepatocytes than hepatocytes derived in vitro. Interspecies chimera-derived hepatocytes could relieve chronic liver fibrosis and reside in the injured liver after transplantation. Our results suggest that interspecies chimera-derived hepatocytes are a potentially reliable source of hepatocytes and can be applied as a therapeutic approach for HT.


Assuntos
Quimera , Hepatócitos , Cirrose Hepática , Fígado , Animais , Hepatócitos/metabolismo , Hepatócitos/citologia , Cirrose Hepática/terapia , Cirrose Hepática/metabolismo , Cirrose Hepática/patologia , Camundongos , Fígado/metabolismo , Fígado/patologia , Ratos , Diferenciação Celular , Camundongos Endogâmicos C57BL , Masculino , Blastocisto/metabolismo , Blastocisto/citologia , Doença Crônica , Células Cultivadas
4.
Theriogenology ; 222: 10-21, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-38603966

RESUMO

Producing chimaeras constitutes the most reliable method of verifying the pluripotency of newly established cells. Moreover, forming chimaeras by injecting genetically modified embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) into the embryo is part of the procedure for generating transgenic mice, which are used for understanding gene function. Conventional methods for generating transgenic mice, including the breeding of chimaeras and tetraploid complementation, are time-consuming and cost-inefficient, with significant limitations that hinder their effectiveness and widespread applications. In the present study, we modified the traditional method of chimaera generation to significantly speed up this process by generating mice exclusively derived from ESCs. This study aimed to assess whether fully ESC-derived mice could be obtained by modulating fibroblast growth factor 4 (FGF4) levels in the culture medium and changing the direction of cell differentiation in the chimaeric embryo. We found that exogenous FGF4 directs all host blastomeres to the primitive endoderm fate, but does not affect the localisation of ESCs in the epiblast of the chimaeric embryos. Consequently, all FGF4-treated chimaeric embryos contained an epiblast composed exclusively of ESCs, and following transfer into recipient mice, these embryos developed into fully ESC-derived newborns. Collectively, this simple approach could accelerate the generation of ESC-derived animals and thus optimise ESC-mediated transgenesis and the verification of cell pluripotency. Compared to traditional methods, it could speed up functional studies by several weeks and significantly reduce costs related to maintaining and breeding chimaeras. Moreover, since the effect of stimulating the FGF signalling pathway is universal across different animal species, our approach can be applied not only to rodents but also to other animals, offering its utility beyond laboratory settings.


Assuntos
Quimera , Fator 4 de Crescimento de Fibroblastos , Animais , Fator 4 de Crescimento de Fibroblastos/genética , Camundongos , Células-Tronco Embrionárias , Camundongos Transgênicos , Embrião de Mamíferos , Diferenciação Celular
5.
Dev Comp Immunol ; 157: 105179, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-38614378

RESUMO

Marine sponges, including the crumb of bread sponge, Hymeniacidon sinapium, display allorejection responses to contact with conspecifics in both experimental and natural settings. These responses have been used to infer immunocompetence in a variety of marine invertebrates. However, larvae and juveniles from several marine sponge species fuse and form chimeras. Some of these chimeras persist, whereas others eventually break down, revealing a period of allogeneic non-responsiveness that varies depending on the species. Alternatively, for H. sinapium, most pairs of sibling post-larvae and juveniles that settle in contact initiate immediate allorecognition and show the same morphological response progression as the adults. This indicates that allorecognition and response occurs during early metamorphosis. Results from H. sinapium and other sponge species, in addition to annotations of sponge genomes, suggest that allorecognition and immunocompetence in sponges are mediated by distinct systems and may become functional at different times during or after metamorphosis for different species. Consequently, allorecognition may not be a good proxy for the onset of immunocompetence.


Assuntos
Larva , Metamorfose Biológica , Poríferos , Animais , Poríferos/imunologia , Poríferos/genética , Larva/crescimento & desenvolvimento , Larva/imunologia , Imunocompetência , Quimera
6.
Science ; 383(6687): 1041, 2024 Mar 08.
Artigo em Inglês | MEDLINE | ID: mdl-38452074

RESUMO

The fish's genomes change so slowly that species separated since the dinosaurs can produce fertile hybrids today.


Assuntos
Evolução Biológica , Quimera , Peixes , Animais , Peixes/classificação , Peixes/genética , Genoma , Reparo do DNA/genética
7.
Neurosci Bull ; 40(6): 849-851, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38492165
9.
Science ; 382(6674): 983-984, 2023 12.
Artigo em Inglês | MEDLINE | ID: mdl-38033062

RESUMO

Genes from second wild grass may have helped propel its success-but scientists don't know how.


Assuntos
Produtos Agrícolas , Hibridização Genética , Zea mays , Zea mays/genética , Produtos Agrícolas/genética , Genes de Plantas , Quimera
10.
J Virol ; 97(10): e0093823, 2023 10 31.
Artigo em Inglês | MEDLINE | ID: mdl-37792003

RESUMO

IMPORTANCE: Human norovirus (HuNoV) is highly infectious and can result in severe illnesses in the elderly and children. So far, there is no effective antiviral drug to treat HuNoV infection, and thus, the development of HuNoV vaccines is urgent. However, NoV evolves rapidly, and currently, at least 10 genogroups with numerous genotypes have been found. The genetic diversity of NoV and the lack of cross-protection between different genotypes pose challenges to the development of broadly protective vaccines. In this study, guided by structural alignment between GI.1 and GII.4 HuNoV VP1 proteins, several chimeric-type virus-like particles (VLPs) were designed through surface-exposed loop grafting. Mouse immunization studies show that two of the designed chimeric VLPs induced cross-immunity against both GI.1 and GII.4 HuNoVs. To our knowledge, this is the first designed chimeric VLPs that can induce cross-immune activities across different genogroups of HuNoV, which provides valuable strategies for the development of cross-reactive HuNoV vaccines.


Assuntos
Infecções por Caliciviridae , Epitopos , Genótipo , Norovirus , Vacinas Virais , Vírion , Animais , Humanos , Camundongos , Infecções por Caliciviridae/imunologia , Infecções por Caliciviridae/prevenção & controle , Infecções por Caliciviridae/virologia , Epitopos/química , Epitopos/genética , Epitopos/imunologia , Imunização , Norovirus/química , Norovirus/classificação , Norovirus/genética , Norovirus/imunologia , Vacinas Virais/química , Vacinas Virais/genética , Vacinas Virais/imunologia , Quimera/genética , Quimera/imunologia , Proteínas do Capsídeo/química , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/imunologia , Vírion/química , Vírion/genética , Vírion/imunologia
11.
Cuad Bioet ; 34(111): 175-188, 2023.
Artigo em Espanhol | MEDLINE | ID: mdl-37804491

RESUMO

Human-animal chimera research has gradually evolved to the present day, in which large projects related to the attempt to solve pathologies that help us human beings to alleviate diseases. However, it must be considered that many of these advances in science imply an important ethical dilemma in many cases, and even more so if we involve people in said experiments. In the present systematic review we sought to identify these ethical problems related to chimeras, as well as possible solutions to them proposed in the literature, including technical means for the realization of less humanized chimeras. A bibliographic search was carried out in the Pubmed, Embase and Medes databases on January 4 th, 2022. The articles that strictly comply with the objectives selected for the completion of the work will be selected. A total of 21 articles makes up our sample, from which ethical problems related to chimeras, possible solutions and technical means to avoid obtaining too humanized chimeras will be extracted. The issues identified in the articles are problems related to animal welfare, acquisition of human traits from chimeras, medical concerns derived from experimentation such as zoonoses, the origin of pluripotential cells for chimera production, the creation of human gametes by said chimeras, neurological chimerism and the moral status of chimeras. This paper provides solutions for these problems, such as the use of suicide genes in human cells that would be activated if they differentiate into neuronal cells or the use of gene editing through the CRISPR/Cas9 mechanism to incapacitate these cells so that they do not differentiate into neuronal cells. The only question that remains elusive to the proposal of solutions is the one related to the potential moral status of chimeras. It is certainly a complex issue given the variety of proposals on the concept of moral status available in literature. It is therefore necessary to bring these proposals closer to reflection on human-animal chimeras in order to initiate a discussion that can shed light on this issue.


Assuntos
Quimera , Ética em Pesquisa , Animais , Humanos
12.
Genome Biol Evol ; 15(9)2023 09 01.
Artigo em Inglês | MEDLINE | ID: mdl-37625795

RESUMO

A range of different genetic architectures underpin local adaptation in nature. Honey bees (Apis mellifera) in the Eastern African Mountains harbor high frequencies of two chromosomal inversions that likely govern adaptation to this high-elevation habitat. In the Americas, honey bees are hybrids of European and African ancestries and adaptation to latitudinal variation in climate correlates with the proportion of these ancestries across the genome. It is unknown which, if either, of these forms of genetic variation governs adaptation in honey bees living at high elevations in the Americas. Here, we performed whole-genome sequencing of 29 honey bees from both high- and low-elevation populations in Colombia. Analysis of genetic ancestry indicated that both populations were predominantly of African ancestry, but the East African inversions were not detected. However, individuals in the higher elevation population had significantly higher proportions of European ancestry, likely reflecting local adaptation. Several genomic regions exhibited particularly high differentiation between highland and lowland bees, containing candidate loci for local adaptation. Genes that were highly differentiated between highland and lowland populations were enriched for functions related to reproduction and sperm competition. Furthermore, variation in levels of European ancestry across the genome was correlated between populations of honey bees in the highland population and populations at higher latitudes in South America. The results are consistent with the hypothesis that adaptation to both latitude and elevation in these hybrid honey bees are mediated by variation in ancestry at many loci across the genome.


Assuntos
Abelhas , Quimera , Animais , Masculino , Aclimatação/genética , Aclimatação/fisiologia , África , Altitude , Abelhas/genética , Abelhas/fisiologia , Quimera/genética , Quimera/fisiologia , Clima , Europa (Continente) , Genômica , Sêmen , América do Sul , Colômbia
13.
Cuad. bioét ; 34(111): 178-188, may.- ago. 2023. tab, graf
Artigo em Espanhol | IBECS | ID: ibc-226232

RESUMO

Las investigaciones con quimeras humano-animales han evolucionado gradualmente hasta día de hoy, en que se plantean grandes proyectos relacionados con el intento de solucionar patologías que nos ayu den a los seres humanos a paliar enfermedades. Sin embargo, se debe de tener en cuenta, que muchos de estos avances científicos llevan implícito un dilema ético importante en muchos casos, y más si se involucra a personas en dichos experimentos. En la presente revisión sistemática se buscó identificar estos problemas éticos relacionados con las quimeras, así como posibles soluciones a los mismos propuestas en la literatura, incluyendo medios técnicos para la realización de quimeras menos humanizadas. Se realizó una búsqueda bibliográfica sistemática en las bases de datos Pubmed, Embase y Medes con fecha 4 de enero de 2022. Se seleccionan los artículos que cumplían estrictamente con los objetivos seleccionados para la realización del trabajo. Un total de 21 artículos componen nuestra muestra, de los cuales se extraen problemas éticos relacionados con las quimeras, posibles soluciones y medios técnicos para evitar la obtención de quimeras demasiado humanizadas. Las cuestiones identificadas en los artículos seleccionados son problemas relacio nados con el bienestar animal, adquisición de rasgos humanos de las quimeras, preocupaciones médicas derivadas de la experimentación como pueden ser las zoonosis, el origen de las células pluripontenciales para la realización de quimeras, la creación de gametos humanos por parte de dichas quimeras, el qui merismo neurológico y el estatus moral de las quimeras. En el trabajo se aportan soluciones para estos problemas, tales como la utilización de genes suicidas en las células humanas que se activarían si estas se diferencian en células neuronales o el uso de la edición genética mediante el mecanismo CRISPR/Cas9 para incapacitar a estas células para que no se diferencien en células neuronales (AU)


Human-animal chimera research has gradually evolved to the present day, in which large projects re lated to the attempt to solve pathologies that help us human beings to alleviate diseases. However, it must be considered that many of these advances in science imply an important ethical dilemma in many cases, and even more so if we involve people in said experiments. In the present systematic review we sought to identify these ethical problems related to chimeras, as well as possible solutions to them proposed in the literature, including technical means for the realization of less humanized chimeras. A bibliographic search was carried out in the Pubmed, Embase and Medes databases on January 4th, 2022. The articles that strictly comply with the objectives selected for the completion of the work will be selected. A total of 21 articles makes up our sample, from which ethical problems related to chimeras, possible solutions and technical means to avoid obtaining too humanized chimeras will be extracted. The issues identified in the articles are problems related to animal welfare, acquisition of human traits from chimeras, medical concerns derived from experimentation such as zoonoses, the origin of pluripotential cells for chimera production, the cre ation of human gametes by said chimeras, neurological chimerism and the moral status of chimeras. This paper provides solutions for these problems, such as the use of suicide genes in human cells that would be activated if they differentiate into neuronal cells or the use of gene editing through the CRISPR/Cas9 mech anism to incapacitate these cells so that they do not differentiate into neuronal cells. The only question that remains elusive to the proposal of solutions is the one related to the potential moral status of chime ras (AU)


Assuntos
Humanos , Experimentação Animal/ética , Experimentação Humana/ética , Ética em Pesquisa , Quimera
14.
Nature ; 619(7971): 811-818, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37407817

RESUMO

RNA viruses have evolved elaborate strategies to protect their genomes, including 5' capping. However, until now no RNA 5' cap has been identified for hepatitis C virus1,2 (HCV), which causes chronic infection, liver cirrhosis and cancer3. Here we demonstrate that the cellular metabolite flavin adenine dinucleotide (FAD) is used as a non-canonical initiating nucleotide by the viral RNA-dependent RNA polymerase, resulting in a 5'-FAD cap on the HCV RNA. The HCV FAD-capping frequency is around 75%, which is the highest observed for any RNA metabolite cap across all kingdoms of life4-8. FAD capping is conserved among HCV isolates for the replication-intermediate negative strand and partially for the positive strand. It is also observed in vivo on HCV RNA isolated from patient samples and from the liver and serum of a human liver chimeric mouse model. Furthermore, we show that 5'-FAD capping protects RNA from RIG-I mediated innate immune recognition but does not stabilize the HCV RNA. These results establish capping with cellular metabolites as a novel viral RNA-capping strategy, which could be used by other viruses and affect anti-viral treatment outcomes and persistence of infection.


Assuntos
Flavina-Adenina Dinucleotídeo , Hepacivirus , Capuzes de RNA , RNA Viral , Animais , Humanos , Camundongos , Quimera/virologia , Flavina-Adenina Dinucleotídeo/metabolismo , Hepacivirus/genética , Hepacivirus/imunologia , Hepatite C/virologia , Reconhecimento da Imunidade Inata , Fígado/virologia , Estabilidade de RNA , RNA Viral/química , RNA Viral/genética , RNA Viral/imunologia , RNA Viral/metabolismo , RNA Polimerase Dependente de RNA/metabolismo , Replicação Viral/genética , Capuzes de RNA/metabolismo
15.
Science ; 380(6648): eabl4997, 2023 06 02.
Artigo em Inglês | MEDLINE | ID: mdl-37262139

RESUMO

Hybridization is widely recognized as promoting both species and phenotypic diversity. However, its role in mammalian evolution is rarely examined. We report historical hybridization among a group of snub-nosed monkeys (Rhinopithecus) that resulted in the origin of a hybrid species. The geographically isolated gray snub-nosed monkey Rhinopithecus brelichi shows a stable mixed genomic ancestry derived from the golden snub-nosed monkey (Rhinopithecus roxellana) and the ancestor of black-white (Rhinopithecus bieti) and black snub-nosed monkeys (Rhinopithecus strykeri). We further identified key genes derived from the parental lineages, respectively, that may have contributed to the mosaic coat coloration of R. brelichi, which likely promoted premating reproductive isolation of the hybrid from parental lineages. Our study highlights the underappreciated role of hybridization in generating species and phenotypic diversity in mammals.


Assuntos
Evolução Biológica , Quimera , Hibridização Genética , Pigmentação , Presbytini , Animais , China , Genoma , Genômica , Presbytini/anatomia & histologia , Presbytini/genética , Isolamento Reprodutivo , Variação Biológica da População , Pigmentação/genética
16.
Science ; 380(6640): 33-34, 2023 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-37023202

RESUMO

The males of an invasive ant species are chimeras of two distinct genetic lineages.


Assuntos
Formigas , Quimera , Espécies Introduzidas , Reprodução , Animais , Masculino , Formigas/genética , Formigas/crescimento & desenvolvimento
17.
Methods Mol Biol ; 2589: 179-193, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36255625

RESUMO

Histone deacetylases are considered promising epigenetic targets for chemical protein degradation due to their diverse roles in physiological cellular functions and in the diseased state. Proteolysis-targeting chimeras (PROTACs) are bifunctional molecules that hijack the cell's ubiquitin-proteasome system (UPS). One of the promising targets for this approach is histone deacetylase 6 (HDAC6), which is highly expressed in several types of cancers and is linked to the aggressiveness of tumors. In the present work, we describe the synthesis of HDAC6 targeting PROTACs based on previously synthesized benzohydroxamates selectively inhibiting HDAC6 and how to assess their activities in different biochemical in vitro assays and in cellular assays. HDAC inhibition was determined using fluorometric assays, while the degradation ability of the PROTACs was assessed using western blot analysis.


Assuntos
Neoplasias , Complexo de Endopeptidases do Proteassoma , Humanos , Desacetilase 6 de Histona/metabolismo , Proteólise , Complexo de Endopeptidases do Proteassoma/metabolismo , Quimera/metabolismo , Ubiquitina/metabolismo , Histona Desacetilases/metabolismo , Ubiquitina-Proteína Ligases/metabolismo
18.
Dev Comp Immunol ; 138: 104554, 2023 01.
Artigo em Inglês | MEDLINE | ID: mdl-36185036

RESUMO

Antimicrobial peptides (AMPs) are gene encoded short peptides which play an important role in the innate immunity of almost all living organisms ranging from bacteria to mammals. Histones play a very important role in defense as precursors to bioactive peptides. The present study is an attempt to decipher the antimicrobial activity of a histone H2A derived peptide, Harriottin-1 from sicklefin chimaera, Neoharriotta pinnata. Analysis in silico predicted the molecule with potent antibacterial and anticancer property. The Harriottin-1 was recombinantly produced and the recombinant peptide rHar-1 demonstrated potent antibacterial activity at 25 µM besides anticancer activity. The study strongly suggests the importance of histone H2A derived peptides as a model for the design and synthesis of potent peptide drugs.


Assuntos
Peptídeos Antimicrobianos , Histonas , Sequência de Aminoácidos , Animais , Antibacterianos , Quimera , Peixes/metabolismo , Histonas/metabolismo , Mamíferos
19.
Hastings Cent Rep ; 52 Suppl 2: S2-S23, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-36484509

RESUMO

This article is the lead piece in a special report that presents the results of a bioethical investigation into chimeric research, which involves the insertion of human cells into nonhuman animals and nonhuman animal embryos, including into their brains. Rapid scientific developments in this field may advance knowledge and could lead to new therapies for humans. They also reveal the conceptual, ethical, and procedural limitations of existing ethics guidance for human-nonhuman chimeric research. Led by bioethics researchers working closely with an interdisciplinary work group, the investigation focused on generating conceptual clarity and identifying improvements to governance approaches, with the goal of helping scholars, funders, scientists, institutional leaders, and oversight bodies (embryonic stem cell research oversight [ESCRO] committees and institutional animal care and use committees [IACUCs]) deliver principled and trustworthy oversight of this area of science. The article, which focuses on human-nonhuman animal chimeric research that is stem cell based, identifies key ethical issues in and offers ten recommendations regarding the ethics and oversight of this research. Turning from bioethics' previous focus on human-centered questions about the ethics of "humanization" and this research's potential impact on concepts like human dignity, this article emphasizes the importance of nonhuman animal welfare concerns in chimeric research and argues for less-siloed governance and oversight and more-comprehensive public communication.


Assuntos
Bem-Estar do Animal , Animais , Humanos , Pesquisa com Células-Tronco , Quimera , Bioética
20.
An. R. Acad. Nac. Farm. (Internet) ; 88(número extraordinario): 117-121, diciembre 2022.
Artigo em Espanhol | IBECS | ID: ibc-225727

RESUMO

Se describe la obtención de embriones quiméricos macaco-humanos por Izpisua y colaboradores (2021) mediante la inyección de células troncales pluripotentes humanas en blastocistos de macaco cultivados ex vivo y se analiza el destino de las líneas celulares humanas en su desarrollo posterior hasta el día 19 después de la fecundación. Se hace una reflexión bioética sobre la relación ciencia-ética en general y sobre dicha investigación en particular. (AU)


The obtention by Izpisua and collaborators (2021) of macaque-human chimeric embryos by microinjection of human pluripotent stem cells into early blastocysts of cynomolgus monkey is described. They studied the competency of human pluripotent stem cells in macaque embryos cultured ex vivo until 19 days post-fertilization. A reflection on these experiments is made from the bioethical point of view. (AU)


Assuntos
Humanos , Quimera , Células-Tronco , Bioética , Genótipo
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