RESUMO
In Brazil, dengue is a public health problem with the occurrence of explosive epidemics. This study reports maternal and fetal deaths due to dengue and which tissues of placenta and umbilical cord were analyzed by molecular methods and immunohistochemistry. The dengue NS3 and NS1 detection revealed the viral presence in different cells from placenta and umbilical cord. In the latter, DENV-2 was detected at a viral titer of 1,02 × 10(4) amounts of viral RNA. It was shown that the DENV markers analyzed here may be an alternative approach for dengue fatal cases investigation, especially involving maternal and fetal death. J. Med. Virol. 88:1448-1452, 2016. © 2016 Wiley Periodicals, Inc.
Assuntos
Vírus da Dengue , Dengue/virologia , Morte Fetal/etiologia , Morte Materna/etiologia , Placenta/virologia , Cordão Umbilical/virologia , Proteínas não Estruturais Virais/isolamento & purificação , Anticorpos Antivirais/imunologia , Antígenos Virais/genética , Brasil/epidemiologia , Dengue/epidemiologia , Vírus da Dengue/química , Vírus da Dengue/genética , Vírus da Dengue/imunologia , Vírus da Dengue/isolamento & purificação , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imuno-Histoquímica , Macrófagos/virologia , Placenta/citologia , Placenta/patologia , Gravidez , RNA Helicases/genética , RNA Helicases/imunologia , RNA Helicases/isolamento & purificação , RNA Viral/genética , RNA Viral/isolamento & purificação , Serina Endopeptidases/genética , Serina Endopeptidases/imunologia , Serina Endopeptidases/isolamento & purificação , Testes Sorológicos , Cordão Umbilical/citologia , Cordão Umbilical/patologia , Proteínas não Estruturais Virais/genética , Proteínas não Estruturais Virais/imunologia , Adulto JovemRESUMO
RNA helicases are ubiquitous enzymes that participate in almost all aspects of RNA processing, including RNA and RNA-protein complex remodelling. In trypanosomatids, which post-transcriptionally regulate gene expression, the formation of different kinds of ribonucleoprotein granules under stress conditions modulates the parasite's RNA metabolism. This paper describes the isolation of a putative DEVH-box RNA helicase produced by promastigotes of Leishmania braziliensis. Using a Cy3-labelled dT30 oligo, FISH showed the localization of this protein to mRNA granules under starvation stress conditions. The central region of the protein was shown to be responsible for this behaviour.