RESUMO
Ultraviolet B (UVB) from the sunlight is a major environmental cause for human skin damages, inducing cell death, inflammation, senescence and even carcinogenesis. The natural flavonoid silibinin, clinically used as liver protectant, has protective effects against UVB-caused skin injury in vivo and in vitro. Silibinin is often classified as a phytoestrogen, because it modulates the activation of estrogen receptors (ERs). However, whether silibinin's estrogenic effect contributes to the skin protection against UVB injury remains to be elucidated. The issue was explored in this study by using the human foreskin dermal fibroblasts (HFF) and human non-malignant immortalized keratinocytes (HaCaT). In HFF, pre-treatment with silibinin rescued UVB-irradiated cells from apoptosis. Interestingly, silibinin increased the whole cellular and nuclear levels of ERα and ERß in UVB-irradiated cells. Activation of ERs by treatment with estradiol elevated the cell survival and reduced apoptosis in UVB-treated cells. ERα agonist increased cell survival, while its antagonist decreased it. ERß agonist also increased cell survival, but the antagonist had no effect on cell survival. Transfection of the cells with the small interfering RNAs (si-RNAs) to ERα or ERß diminished the protective effect of silibinin on UVB-irradiated cells. In UVB-treated HaCaT cells, both ERα and ERß were increased by silibinin treatment. Inhibition of activation and expression of ERα or ERß by specific antagonists and si-RNAs, respectively, reduced cell survival in UVB-treated HaCaT cells regardless of silibinin treatment. Taken together, it is summarized that silibinin up-regulates both ERα and ERß pathways in UVB-treated dermal HFF cells and epidermal HaCaT cells, leading to protection of skin from UVB-damage.
Assuntos
Fibroblastos/efeitos da radiação , Substâncias Protetoras/química , Receptores de Estrogênio/antagonistas & inibidores , Silibina/química , Apoptose/efeitos da radiação , Fibroblastos/citologia , Células HaCaT , Humanos , Substâncias Protetoras/farmacologia , RNA Interferente Pequeno/metabolismo , RNA Interferente Pequeno/efeitos da radiação , Receptores de Estrogênio/efeitos da radiação , Transdução de Sinais , Silibina/farmacologia , Pele , Raios Ultravioleta , Regulação para Cima/efeitos da radiaçãoRESUMO
Genotoxic agents produce numerous cellular responses that are principally dedicated to maintain or restore DNA integrity. In human cells, nucleotide excision repair (NER) is one of the major pathways for the repair of DNA damage such as ultraviolet (UV) radiation-induced lesions. Endocrine disrupting compounds are environmental contaminants that interfere with the function of the endocrine system. Among them, the natural estrogen 17beta-estradiol (E(2)) exhibits the most potent activity. Some proteins directly or indirectly involved in NER also fulfill other functions such as transcription, DNA damage checkpoints or cell cycle. Moreover, steroids such as E(2) are believed to interact with a large number of proteins including some involved in NER and DNA damage checkpoint control. We therefore investigated the potential modulation of genotoxic stress-cells responses by E(2) treatment. Estrogen receptor (ER)-positive human breast cancer cells were submitted to E(2) before and/or after UVB irradiation and thereafter the repair kinetics of UV-induced DNA damage were evaluated. We report here that the repair rate of UVB-induced DNA damage is enhanced when cells are submitted to an estrogenic stimulation. Moreover, our results suggest that this response could be mediated by cell cycle regulatory proteins in a p53-independent manner.
Assuntos
Neoplasias da Mama/metabolismo , Dano ao DNA , Reparo do DNA/efeitos dos fármacos , Estradiol/farmacologia , Receptores de Estrogênio/metabolismo , Raios Ultravioleta , Proteínas de Ciclo Celular/metabolismo , Dano ao DNA/fisiologia , Dano ao DNA/efeitos da radiação , Relação Dose-Resposta à Radiação , Feminino , Genes p53/fisiologia , Genes p53/efeitos da radiação , Humanos , Neoplasias Hormônio-Dependentes , Receptores de Estrogênio/efeitos da radiação , Transfecção , Células Tumorais CultivadasRESUMO
Chemotherapy or irradiation treatment induces breast cancer cell apoptosis, but this can be limited by estradiol (E2) through unknown mechanisms. To investigate this, we subjected estrogen receptor-expressing human breast cancer cells (MCF-7 and ZR-75-1) to paclitaxel (taxol) or to UV irradiation. Marked increases in cell apoptosis were induced, but these were significantly reversed by incubation with E2. Taxol or UV stimulated c-Jun N-terminal kinase (JNK) activity, which was inhibited by E2. Expression of a dominant-negative Jnk-1 protein strongly prevented taxol- or UV-induced apoptosis, whereas E2 inhibition of apoptosis was reversed by expression of constituitively active Jnk-1. As targets for participation in apoptosis, Bcl-2 and Bcl-xl were phosphorylated in response to JNK activation by taxol or UV; this was prevented by E2. Taxol or UV activated caspase activity in a JNK-dependent fashion and caused the cleavage of procaspase-9 to caspase-9, each inhibited by E2. Independently, the steroid also activated extracellular signal-regulated protein kinase activity, which contributed to the antiapoptotic effects. We report novel and rapid mechanisms by which E2 prevents chemotherapy or radiation-induced apoptosis of breast cancer, probably mediated through the plasma membrane estrogen receptor.
Assuntos
Apoptose/fisiologia , Receptores de Estrogênio/fisiologia , Apoptose/efeitos dos fármacos , Neoplasias da Mama , Caspase 9 , Caspases/metabolismo , Membrana Celular/fisiologia , Precursores Enzimáticos/metabolismo , Estradiol/farmacologia , Feminino , Humanos , Proteínas Quinases JNK Ativadas por Mitógeno , Proteína Quinase 8 Ativada por Mitógeno , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Proteínas Quinases Ativadas por Mitógeno/efeitos da radiação , Paclitaxel/farmacologia , Fosforilação , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Receptores de Estrogênio/efeitos dos fármacos , Receptores de Estrogênio/efeitos da radiação , Proteínas Recombinantes/metabolismo , Transfecção , Células Tumorais Cultivadas , Raios Ultravioleta , Proteína bcl-XRESUMO
Ionizing radiation in doses of 0.5 and 1.0 Gy modifies cytosol estrogen- and progestin-receptor complexes decreasing their acception by hepatocyte nuclei in the liver of gamma-irradiated female rats.
Assuntos
Hepatócitos/metabolismo , Hepatócitos/efeitos da radiação , Receptores de Estrogênio/efeitos da radiação , Receptores de Progesterona/efeitos da radiação , Animais , Núcleo Celular/metabolismo , Núcleo Celular/efeitos da radiação , Citosol/metabolismo , Citosol/efeitos da radiação , Feminino , Cinética , Ratos , Ratos WistarRESUMO
Chicken ovalbumin upstream promoter-transcription factor (COUP-TF) was identified as a low abundance protein in bovine uterus that co-purified with estrogen receptor (ER) in a ligand-independent manner and was separated from the ER by its lower retention on estrogen response element (ERE)-Sepharose. In gel mobility shift assays, COUP-TF bound as an apparent dimer to ERE and ERE half-sites. COUP-TF bound to an ERE half-site with high affinity, Kd = 1.24 nM. In contrast, ER did not bind a single ERE half-site. None of the class II nuclear receptors analyzed, i.e. retinoic acid receptor, retinoid X receptor, thyroid receptor, peroxisome proliferator-activated receptor, or vitamin D receptor, were constituents of the COUP-TF.DNA binding complex detected in gel mobility shift assays. Direct interaction of COUP-TF with ER was indicated by GST "pull-down" and co-immunoprecipitation assays. The nature of the ER ligand influenced COUP-TF-ERE half-site binding. When ER was liganded by the antiestrogen 4-hydroxytamoxifen (4-OHT), COUP-TF-half-site interaction decreased. Conversely, COUP-TF transcribed and translated in vitro enhanced the ERE binding of purified estradiol (E2)-liganded ER but not 4-OHT-liganded ER. Co-transfection of ER-expressing MCF-7 human breast cancer cells with an expression vector for COUP-TFI resulted in a dose-dependent inhibition of E2-induced expression of a luciferase reporter gene under the control of three tandem copies of EREc38. The ability of COUP-TF to bind specifically to EREs and half-sites, to interact with ER, and to inhibit E2-induced gene expression suggests COUP-TF regulates ER action by both direct DNA binding competition and through protein-protein interactions.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Estrogênios/farmacologia , Expressão Gênica/efeitos dos fármacos , Ovalbumina/genética , Receptores de Estrogênio/metabolismo , Receptores de Glucocorticoides/metabolismo , Fatores de Transcrição/metabolismo , Animais , Anticorpos Monoclonais/metabolismo , Fator I de Transcrição COUP , Bovinos , Galinhas , Sequência Consenso , DNA/metabolismo , Genes Reporter , Humanos , Cinética , Receptores Citoplasmáticos e Nucleares/imunologia , Receptores Citoplasmáticos e Nucleares/metabolismo , Receptores de Estrogênio/efeitos da radiação , Proteínas Recombinantes/metabolismo , Ativação Transcricional/efeitos dos fármacos , Células Tumorais Cultivadas , Raios Ultravioleta , Receptor ERRalfa Relacionado ao EstrogênioRESUMO
DNA damage induced by the radioactive decay of 125I-estrogen (125I-VME2) in an estrogen receptor expressing CHO cell line, CHO-ER, was measured. 125I-VME2 targeted 125I atoms proximal to DNA estrogen response elements (EREs). 125I decays were accumulated at -135 degrees C, and thereafter assayed by alkaline and neutral filter elution techniques to measure DNA single strand break (ssb) and double strand break (dsb) induction respectively. Increasing DNA damage (both ssbs and dsbs) was detected after exposure of cells to increasing concentrations of 125I-VME2. DNA ssb and dsb dose-response curves for 125I-VME2 were multiphasic. The rates of DNA damage induction by the decay of 125I-VME2 was determined by comparing slopes of all data or by comparing initial slopes. DNA ssb induction per 125I-VME2 decay was approximately 2 times greater compared with DNA dsb induction. 125I-VME2 decay induced approximately 4-8 times more DNA dsbs than 125IUdR decay.
Assuntos
Dano ao DNA , DNA/efeitos da radiação , Estrogênios/farmacologia , Radioisótopos do Iodo , Transferência Linear de Energia , Animais , Células CHO , Cricetinae , DNA/metabolismo , DNA de Cadeia Simples/efeitos da radiação , Relação Dose-Resposta à Radiação , Radioisótopos do Iodo/metabolismo , Receptores de Estrogênio/efeitos da radiaçãoRESUMO
We studied the effect of 123I-labeled estrogen (123I-E) in estrogen receptor (ER)-rich cells in culture and in cell free model systems in vitro to elucidate the nature of the radiotoxicity for ER + cells of estrogens containing nuclides which emit Auger electrons. In cells the 123I-E caused a dose-dependent, unlabeled estrogen-inhibitable induction of chromosome aberrations. A dose of about 1000 decays per cell, which is approximately the mean lethal dose for these cells, resulted in an average of 1 chromosome break per cell. This supports the hypothesis that the lethal lesion induced by 123I-E is a chromosome break. Incubation of 123I-E/ER complex, but not 123I-E alone, with 27-mer duplex estrogen response element (ERE) DNA produced a dose-dependent cleavage of the ERE. However, we were unable to detect any fragmentation of either the 66 kDa full length ER in cell extracts or a purified 31 kDa hormone binding domain when incubated with excess 123I-E. Thus it appears that 123I-E effects its radiotoxicity by binding to ER, associating with ERE DNA and, by directing high LET radiation to DNA, inducing lethal chromosome breaks.
Assuntos
Aberrações Cromossômicas , Dano ao DNA , Estrogênios/farmacologia , Radioisótopos do Iodo , Transferência Linear de Energia , Receptores de Estrogênio/efeitos da radiação , Animais , Células CHO/química , Células CHO/efeitos da radiação , Cricetinae , Estrogênios/metabolismo , Radioisótopos do Iodo/metabolismo , Doses de RadiaçãoRESUMO
PURPOSE: Studies on radiation-induced changes in gene expression are likely to be very important in developing a better understanding of cellular responses to ionizing radiation. While there is some information on the activation of cellular signal transduction pathways after radiation, few late reacting target genes have been identified. This study focuses on the characterization of expression modulation of two critical growth regulatory genes, estrogen receptor and epidermal growth factor-receptor in malignant mammary epithelial cells in response to single and repeated ionizing radiation exposures. METHODS AND MATERIALS: MCF-7 cells were used for single radiation exposure (2-50 Gy) experiments and MCF-IR-3 cells, generated by exposure to cumulative doses of 60 Gy in 2 Gy fractions, respectively, were used to study the effects of repeated exposures. Steady-state messenger ribonucleic acid levels for estrogen receptor, epidermal growth factor-receptor, and transforming growth factor-alpha were determined by ribonucleic acid protection experiments. Estrogen receptor and epidermal growth factor-receptor protein expression was quantitated by competitive binding studies with 3H-estradiol and 125I-EGF. RESULTS: MCF-IR-3 cells showed a permanent three-fold down-regulation of the estrogen receptor messenger ribonucleic acid and protein, while epidermal growth factor-receptor was upregulated about nine-fold. Epidermal growth factor-receptor was substantially up-regulated in MCF-7 cells, at both the mRNA and protein levels, within 24 h of a single 2 Gy exposures, while there was a two-fold concomitant increase in transforming growth factor-alpha messenger ribonucleic acid expression. A decrease in estrogen receptor messenger ribonucleic acid and protein was suggested only after higher doses of single radiation exposures. CONCLUSION: Single and repeated radiation exposures modulate the expression of two critical growth promoting genes, estrogen receptor and epidermal growth factor-receptor, in MCF-7 cells. The inverse expression of estrogen receptor and epidermal growth factor-receptor established for estrogen receptor-positive malignant mammary epithelial cells is maintained in MCF-7 cells after single and repeated exposures suggesting that radiation acts through common regulatory circuits and may modulate the cellular phenotype.
Assuntos
Neoplasias da Mama/radioterapia , Neoplasias da Mama/ultraestrutura , Receptores ErbB/genética , Receptores ErbB/efeitos da radiação , Regulação Neoplásica da Expressão Gênica/efeitos da radiação , Receptores de Estrogênio/genética , Receptores de Estrogênio/efeitos da radiação , Neoplasias da Mama/patologia , Ciclo Celular/efeitos da radiação , Sobrevivência Celular/efeitos da radiação , Relação Dose-Resposta à Radiação , Receptores ErbB/metabolismo , Expressão Gênica/efeitos da radiação , Humanos , Fenótipo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Mensageiro/efeitos da radiação , Receptores de Estrogênio/metabolismo , Fator de Crescimento Transformador alfa/genética , Células Tumorais Cultivadas/efeitos da radiação , Regulação para Cima/fisiologia , Regulação para Cima/efeitos da radiaçãoRESUMO
Characterization of the DNA-binding state of the rat uterine estrogen receptor was examined by UV cross-linking using [32P]5-bromo-2'-deoxyuridine-substituted 25-base pair synthetic oligonucleotide containing a Xenopus vitellogenin A2 estrogen response element (BrdUVRE). After UV irradiation of the receptor-BrdUVRE complexes, they were analyzed by SDS-polyacrylamide gel electrophoresis. In the nuclear estradiol-, ICI164, 384- and 4-hydroxytamoxifen-receptor complexes, the bands corresponding to the mobility of the receptor homodimer were observed. In a molybdate stabilized soluble receptor, we observed the bands with the slower mobility than the receptor homodimers.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Proteínas de Ligação a DNA/efeitos da radiação , DNA/metabolismo , DNA/efeitos da radiação , Receptores de Estrogênio/metabolismo , Receptores de Estrogênio/efeitos da radiação , Raios Ultravioleta , Útero/metabolismo , Útero/ultraestrutura , Animais , Sequência de Bases , Bromodesoxiuridina/metabolismo , Eletroforese , Feminino , Dados de Sequência Molecular , Molibdênio/farmacologia , Radioisótopos de Fósforo , Ratos , Ratos Wistar , Solubilidade , Especificidade por Substrato , Útero/efeitos da radiação , Vitelogeninas/metabolismo , XenopusRESUMO
Strategies for diagnosis and therapy in which sex steroid receptor ligands serve as carriers for radionuclides are attractive because a high incidence of carcinomas of the female genital tract and the breast that are seen clinically have an abundant expression of one or more of the receptor proteins. A radiohalogenated estrogen receptor (ER) ligand, 16 alpha-[123I]iodo-17 beta-estradiol [123I]E, has met clinical criteria for receptor-mediated diagnostic imaging. Its [125]I-labeled sister nuclide derivative [125I]E decays by orbital electron capture with emission of very-low-energy (Auger) electrons, which gives this latter radiohalogen the potential to serve in pharmaceuticals for radiotherapy; as examples, [125I]deoxyuridine, when incorporated into the DNA molecule, or [125I]E, when bound to the receptor within ER-rich tumor cells, are both cytotoxic in vitro. Whereas the mechanisms and subcellular changes that accompany the cytotoxicity from [125I]deoxyuridine are well documented in the form of aberrations and breaks in the cellular DNA, the effects at the subcellular level causing the cytotoxicity of the sex steroid receptor ligand [125I]E have not been characterized and are the focus of our study. We found that in a standard colony-forming assay the addition of [125I]E to the cultures decreased the survival rate of ER-positive MCF-7 cells in a dose-dependent manner. The decreased survival rate was prevented by the addition of competing excess radioinert ER ligand (diethylstilbestrol); [125I]E did not reduce survival in ER-negative MCF-7 cells. The [125I]E-induced and ER-mediated cytotoxicity was accompanied by aberrations in the DNA components of the nuclei of the cells. These included chromatid and chromosome breaks, gaps, and tri-radial chromosome formation. Our findings add plausibility and credence to the notion that the cytotoxicity imparted by Auger-electron-emitting radioligands for sex steroid receptors is in part attributable to radiodecay that causes double-stranded breakage of DNA.
Assuntos
Dano ao DNA/efeitos da radiação , Estradiol/análogos & derivados , Radioisótopos do Iodo , Receptores de Estrogênio/efeitos da radiação , Cromossomos/efeitos da radiação , Ensaio de Unidades Formadoras de Colônias , Estradiol/farmacologia , Humanos , Ensaio Radioligante , Células Tumorais CultivadasRESUMO
To assess the feasibility of using estrogen receptor-directed therapy with Auger electron-emitting ligands for therapy of estrogen receptor (ER)-containing cancers, we synthesized and evaluated the radiotoxicity of several 123I-labeled estrogens to specifically kill ER+ cells in culture. Auger electrons have been previously shown to be of short range, generally less than the dimensions of a cell, so that to use them therapeutically a mechanism is needed to deliver the Auger electron-emitting nuclide to the vicinity of the DNA. Since it is now well established that the estrogen receptor, when bound to estrogen, forms a high affinity association with distinct estrogen response elements in the DNA, we wished to test the hypothesis that a short exposure of cells to a 123I-labeled estrogen would be specifically radiotoxic to ER+ cells, and that the decays per cell needed for cell killing would be compatible with reasonable levels of receptor occupancy. Using the halodestannylation reaction with tributyl tin precursors of several estrogens and commercially available iodine-123, we prepared the iodoestrogens, E-17 alpha(-)[123I]-iodo-11 beta-methoxyestradiol and 2(-)[123I]iodo-1,1-bis(4-hydroxyphenyl)--2-phenylethylene, at high specific activities, in several cases at essentially the specific activity of 123I itself, 240,000 Ci/mmol. When various concentrations of either of the 123I-labeled estrogens were incubated for 1 h with a subline of ER+ Chinese hamster ovary cells and the washed cells plated for survival assays, a dose-dependent, unlabeled estradiol-inhibitable reduction in survival was observed. In contrast, Chinese hamster ovary cells not expressing estrogen receptor showed little sensitivity to the radiotoxicity of the 123I-labeled estrogens. Calculations based on the assayed residence time of the iodoestrogens in the cells indicate that several hundred decays per cell are sufficient to kill cells.
Assuntos
Elétrons , Radioisótopos do Iodo/toxicidade , Receptores de Estrogênio/efeitos da radiação , Animais , Antineoplásicos/toxicidade , Células CHO/metabolismo , Células CHO/efeitos da radiação , Morte Celular/efeitos da radiação , Cricetinae , Relação Dose-Resposta à Radiação , Estradiol/análogos & derivados , Estradiol/toxicidade , Radioisótopos do Iodo/uso terapêutico , Neoplasias Hormônio-Dependentes/metabolismo , Neoplasias Hormônio-Dependentes/radioterapia , Receptores de Estrogênio/metabolismo , Sensibilidade e Especificidade , Estilbenos/toxicidadeRESUMO
Whole MCF-7 human breast-cancer cells were irradiated at - 78 degrees C in a calibrated Gammacel 60Co irradiator. Freezing or storing conditions induce neither an alteration of the viability of cells nor a change in estradiol binding activity. Hexosaminidase was used as internal marker, and we measured the radiation inactivation size (RIS) of the estrogen receptor in whole cells. After various cell treatments, the estradiol binding unit always presents a molecular mass of 25 kDa. This value, which corresponds to the size of the defined hormone binding domain of the estrogen receptor, suggests that the energy delivered to the protein by the radiation is efficient to inactivate estradiol binding only when the hit occurs directly in the smaller hormone binding domain.
Assuntos
Neoplasias da Mama/química , Receptores de Estrogênio/efeitos da radiação , Sítios de Ligação/efeitos da radiação , Sobrevivência Celular/efeitos da radiação , Estradiol/metabolismo , Humanos , Cinética , Radiação Ionizante , Receptores de Estrogênio/química , Receptores de Estrogênio/metabolismo , Temperatura , Células Tumorais Cultivadas , beta-N-Acetil-Hexosaminidases/metabolismoRESUMO
To investigate the effect of ligand (be it hormone, antihormone, or no hormone) on the interaction between estrogen receptor (ER) and chromatin, we have used formaldehyde as a cross-linking agent in intact MCF-7 human breast cancer cells. After a 1- to 2-h hormone treatment, the cells are exposed for 8 min to formaldehyde, which is added directly to their culture medium to minimize environmental perturbation. Nuclei are prepared from formaldehyde-treated cells and their contents are fractionated on CsCl density gradients to separate DNA-protein complexes from free protein. Peak gradient fractions are assayed for the presence of specific proteins by immunoblot of sodium dodecyl sulfate-polyacrylamide gel patterns. Using this approach, we find that 0.15% formaldehyde is optimal for cross-linking ER to chromatin. We detect ER and the large subunit of RNA polymerase II with DNA from formaldehyde-treated, but not from untreated cells. On the other hand, actin (a cytoplasmic protein) and small nuclear ribonucleoprotein particle proteins (nuclear RNA binding proteins) are not cross-linked to DNA. Therefore, cross-linking appears to be selective and fractionation is efficient. Interestingly, we detect similar levels of ER (as well as RNA polymerase II) with DNA from formaldehyde-treated cells, regardless of whether the cells are preexposed to estrogen (17 beta-estradiol at 10(-8) M), antiestrogen (ICI 164,384 at 10(-7) or 10(-6) M), or no hormone. These results, using covalent cross-linking in intact cells, indicate that both ligand-occupied and unoccupied ER are associated with chromatin.
Assuntos
Neoplasias da Mama/genética , Cromatina/metabolismo , Reagentes de Ligações Cruzadas/metabolismo , DNA/metabolismo , Receptores de Estrogênio/genética , Ribonucleoproteínas/genética , Centrifugação com Gradiente de Concentração , Cromatina/efeitos dos fármacos , Estradiol/análogos & derivados , Antagonistas de Estrogênios/farmacologia , Formaldeído/farmacologia , Humanos , Ligantes , Alcamidas Poli-Insaturadas , RNA Polimerase II/genética , Receptores de Estrogênio/efeitos dos fármacos , Receptores de Estrogênio/efeitos da radiação , Células Tumorais Cultivadas , Raios UltravioletaRESUMO
MCF-7 human breast cancer cells were submitted to the tritiated antiestrogen tamoxifen aziridine, frozen at -170 degrees C, stored and irradiated at -78 degrees C in a calibrated Gammacell 60Co irradiator. A three-step protein extraction procedure provided protein samples for the determination of the target size (TS) of the covalently labelled estrogen receptor (ER). From the TS it is shown that ER bound to an antiestrogen was, in whole cells, part of a 265 kDa polypeptide structure if measured in MCF-7 cells at subconfluency, or of a 360 kDa species in superconfluent cells.
Assuntos
Neoplasias da Mama/metabolismo , Receptores de Estrogênio/metabolismo , Radioisótopos de Cobalto , Eletroforese em Gel de Poliacrilamida , Humanos , Peso Molecular , Receptores de Estrogênio/isolamento & purificação , Receptores de Estrogênio/efeitos da radiação , Tamoxifeno/análogos & derivados , Tamoxifeno/metabolismo , Trítio , Células Tumorais CultivadasRESUMO
The present investigation reaffirms our observation that removal of a C3H mouse mammary adenocarcinoma results in a perturbation of tumor cells in a metastatic focus. An increase occurs in the proportion of cells undergoing DNA synthesis (labeling index, LI), and a decrease occurs in the proportion demonstrating estrogen receptor (ER index; ERI). The changes are transient but of sufficient duration and magnitude to produce an increase in the size of a distant tumor. This study was conducted to determine whether cytoreduction of a primary tumor by irradiation would produce a similar change in metastatic tumor cells and whether preoperative radiation would obtund the effect of primary tumor removal. The administration of a maximum tolerated dose of radiation (50 Gy) to a primary tumor produced a significant (p less than 0.001) increase in LI and decrease in ERI of a lesser magnitude than that observed following surgical removal of the primary tumor, but still sufficient to enhance the growth of a metastatic focus. Whereas, there was almost a 50% increase in LI in a metastasis 1 and 3 days following removal of a primary tumor the increase was only 13% three days after radiation. There was a 20% decrease in ERI 3 days following radiation and a 37% decrease at that time following tumor removal. Preoperative irradiation of a primary tumor 1, 3, or 5 days prior to tumor removal, obtunds the increase in LI and decrease in ERI following operation. Radiation the day before surgery was most effective because the changes in a distant focus occurring as a result of the radiation and of the surgery were prevented. The clinical relevance of these observations deserves further consideration.
Assuntos
Replicação do DNA/efeitos da radiação , Neoplasias Mamárias Experimentais/radioterapia , Receptores de Estrogênio/efeitos da radiação , Animais , Divisão Celular/efeitos da radiação , Feminino , Cinética , Neoplasias Mamárias Experimentais/análise , Neoplasias Mamárias Experimentais/patologia , Camundongos , Camundongos Endogâmicos C3H , Metástase Neoplásica , Receptores de Estrogênio/análiseRESUMO
Estradiol and progesterone receptor levels were measured in 130 patients with stage III breast tumors before treatment and following preoperative radiation or chemotherapy. The data were evaluated versus the morphologic features of posttreatment pathomorphosis of tumor. Standard fractionated radiation (total dose of 70 Gy) was followed by pronounced postradiation pathomorphosis and a decrease in the level and incidence of steroid receptors in 72.7-87.5%. The essentially unchanged receptor profile of tumor following large-fraction (total dose-20 Gy) irradiation as well as presence of estradiol and progesterone receptors in the originally receptor-negative neoplasms after chemotherapy were matched by a slight degree of pathomorphosis.
Assuntos
Neoplasias da Mama/patologia , Progesterona/metabolismo , Receptores de Estrogênio/análise , Receptores de Progesterona/análise , Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Biópsia , Mama/análise , Mama/patologia , Neoplasias da Mama/análise , Neoplasias da Mama/terapia , Terapia Combinada , Estrogênios/metabolismo , Feminino , Humanos , Cuidados Pré-Operatórios , Dosagem Radioterapêutica , Receptores de Estrogênio/efeitos dos fármacos , Receptores de Estrogênio/efeitos da radiação , Receptores de Progesterona/efeitos dos fármacos , Receptores de Progesterona/efeitos da radiaçãoRESUMO
Human mammary tumor cells in continuous culture (MCF-7 cells) are hormone- and radiosensitive. The interaction of both factors is analyzed. Ionizing irradiation lowers the concentration of both the estradiol and progesterone receptors per cell. The reduction is dose dependent. However, the effects on the cytoplasmic and nuclear forms of the receptors are not similar. For the estradiol receptor, an accumulation in the nuclear fraction is observed 48 hr after irradiation when no appreciable amounts of estrogens are present. After administration of 10(-8) M estradiol, the cytoplasmic clearance is comparable to the unirradiated controls. However, nuclear accumulation is impaired. The processing of the nuclear estrogen receptor remains identical. Nuclear progesterone receptor is not significantly increased due to irradiation in the absence of progestins. Cytoplasmic decrease after incubation with progestins is unaffected. Again, nuclear accumulation is impaired in contrast to the unchanged processing of the nuclear form of the progesterone receptor. A decrease in "nuclear acceptor sites" for both receptors after irradiation may be an explanation for these observations. No significant effects of ionizing irradiation are observed in the initial steps of steroid hormone action.
Assuntos
Neoplasias da Mama/metabolismo , Receptores de Estrogênio/efeitos da radiação , Receptores de Progesterona/efeitos da radiação , Neoplasias da Mama/patologia , Divisão Celular/efeitos dos fármacos , Divisão Celular/efeitos da radiação , Núcleo Celular/metabolismo , Citosol/metabolismo , DNA de Neoplasias/análise , Estradiol/metabolismo , Feminino , Humanos , Cinética , Proteínas de Neoplasias/análise , Promegestona/metabolismo , Receptores de Estrogênio/metabolismo , Receptores de Progesterona/metabolismoRESUMO
The effects of ionizing irradiation on the sedimentation coefficients of both estrogen receptor (ER) and progesterone receptor (PgR) have been examined in comparison to the effects of proteolysis. DMBA-induced rat mammary tumors were subjected to a treatment of 20 Gy and the ER and PgR concentrations were determined at different time intervals after irradiation. On a 5-20% sucrose gradient the ER sedimented as 9-11 and 4-5 S molecular forms, while PgR sedimented as a small 8-9 S peak and a major 4-5 S peak. Radiotherapy particularly reduced the 4-5 S sedimentation peaks of both receptors but did not initiate any new sedimentation forms. Although the 4-5 S ER receptor concentrations remained low, both progesterone receptor forms appeared to recover by 60 days after treatment. As these effects could be due to the release of proteolytic enzymes following irradiation of tumors, the receptors from untreated tumors were exposed to different concentrations of trypsin. The effects of trypsin were identical for ER and for PgR, and proved to be dependent on the trypsin concentration. Only concentrations of trypsin up to 30 micrograms/ml resulted in a reduction of 9-11 S ER or 8-9 S PgR forms which was accompanied by a simultaneous increase in the 4-5 S peaks, resulting in no change in total binding sites. Still higher trypsinization (300-3000 micrograms/ml) also reduced the 4-5 S ER and PgR fractions. In the presence or the absence of sodium molybdate, a stabilizer of the faster sedimenting forms of the receptor, no alterations were observed in the position of, or the total number of binding sites of, the sucrose gradient fractions from control or irradiated tumors. The irradiation effects appear to be due either to damage of the cytosolic ER receptor, thereby preventing its participation in the induction of de novo synthesis of ER and PgR, or to the non-specific damage of transcription and/or translation systems.