Assuntos
Antibióticos Antituberculose/efeitos adversos , Autoanticorpos/análise , Doenças Autoimunes/tratamento farmacológico , Córnea/efeitos dos fármacos , Opacidade da Córnea/induzido quimicamente , Fatores Imunológicos/administração & dosagem , Interferon gama/imunologia , Infecção por Mycobacterium avium-intracellulare/tratamento farmacológico , Rifabutina/efeitos adversos , Rituximab/administração & dosagem , Adulto , Antibióticos Antituberculose/análise , Doenças Autoimunes/diagnóstico , Doenças Autoimunes/imunologia , Córnea/química , Córnea/patologia , Opacidade da Córnea/diagnóstico , Opacidade da Córnea/metabolismo , Feminino , Humanos , Hospedeiro Imunocomprometido , Fatores Imunológicos/efeitos adversos , Infecção por Mycobacterium avium-intracellulare/diagnóstico , Infecção por Mycobacterium avium-intracellulare/microbiologia , Rifabutina/análise , Fatores de Risco , Rituximab/efeitos adversos , SíndromeRESUMO
Capillary zone electrophoresis (CZE) was used for determination of rifabutin (RFB), an anti-tuberculosis antibiotic drug, in various pharmaceutical formulations. Apart from that, simultaneous determination of RFB and human serum albumin (HSA) was performed. Electrophoretic behaviour of RFB was examined at various pH levels. CE conditions: a quartz capillary tube (internal diameter 75mm, effective length 50cm, total length 60cm), the capillary temperature was 25°Ð¡, the voltage applied to the capillary tube was +20kV, the UV detection wavelength was 214nm, hydrodynamic injection of the sample was performed at 30mbar for 5s, tetraborate buffer solution (0.01Ð, ÑÐ9.2). The obtained results are characterized by high efficiency (number of theoretical plates up to 260,000) and sufficient sensitivity (LOQ starting from 0.02µg/ml for RFB). The obtained data are in good accord with both HPLC results (for RFB) and spectrophotometry (for HSA).