RESUMO
The aim of our work was to identify the anthraquinone derivatives in the underground organs (root-stock and root) of the madder root live plant sortiment Rubia tinctorium L, furthermore to isolate populations the anthraquinone compostitions of which are optimal from therapeutical point of view. In the course of the examinations, we studied the anthraquinone composition in root-stock and root samples of plants originating from 11 different habitats. The identification of the anthraquinone derivatives we carried out after the hydrolysis of these samples with HCl solution, by HPLC method and by use of UV-VIS spectrophotometric and mass spectrometric detection. In the samples we identified 6 anthraquinone-aglicones. The main components were alizarin (9.6-21.8 mg/g), purpurin (3.7-12.3 mg/g), and lucidin (1.8-5.7 mg/g). The total anthraquinone-derivative content varied between 15.6 and 39.4 mg/g. After the evaluation of the results it can be stated that from therapeutical point of view the most favourable biggest ratio of total anthraquinone amount/lucidin amount (11.97) and one of the biggest total anthraquinone-derivative contents (38.1 mg/g) are found in the Rubia tinctorium sample marked R02 originating from Aachen (Germany). These results enable us at the same time to find--through the establishment of further collections and through the exploration of the biodiversity of these--basic material with more favourable composition than at present, especially in relation to lucidin derivatives which are risk factors.
