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1.
PLoS One ; 17(2): e0264226, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35213573

RESUMO

The higher airborne microbial concentration in indoor areas might be responsible for the adverse indoor air quality, which relates well with poor respiratory and general health effects in the form of Sick building syndromes. The current study aimed to isolate and characterize the seasonal (winter and spring) levels of culturable bio-aerosols from indoor air, implicating human health by using an epidemiological health survey. Microorganisms were identified by standard macro and microbiological methods, followed by biochemical testing and molecular techniques. Sampling results revealed the bacterial and fungal aerosol concentrations ranging between (300-3650 CFU/m3) and (300-4150 CFU/m3) respectively, in different microenvironments during the winter season (December-February). However, in spring (March-May), bacterial and fungal aerosol concentrations were monitored, ranging between (450-5150 CFU/m3) and (350-5070 CFU/m3) respectively. Interestingly, Aspergillus and Cladosporium were the majorly recorded fungi whereas, Staphylococcus, Streptobacillus, and Micrococcus found predominant bacterial genera among all the sites. Taken together, the elevated levels of bioaerosols are the foremost risk factor that can lead to various respiratory and general health issues in additional analysis, the questionnaire survey indicated the headache (28%) and allergy (20%) were significant indoor health concerns. This type of approach will serve as a foundation for assisting residents in taking preventative measures to avoid exposure to dangerous bioaerosols.


Assuntos
Microbiologia do Ar , Poluição do Ar em Ambientes Fechados , Aspergillus/classificação , Bactérias , Cladosporium/classificação , Síndrome do Edifício Doente/microbiologia , Bactérias/classificação , Monitoramento Ambiental , Humanos , Estações do Ano
2.
Innate Immun ; 27(1): 15-22, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33287602

RESUMO

In this comparative study, serum complement system antimicrobial activity was measured from 159 serum samples, taken from individuals from microbe-damaged (70 samples) and from reference buildings (89 samples). Antimicrobial activity was assessed using a probe-based bacterial Escherichia coli-lux bioluminescence system and comparison was made at a group level between the experimental and reference group. The complement activity was higher in users of microbe-damaged buildings compared with the reference group and the significant (P < 0.001) increase in activity was found in the classical reaction pathway. This study strengthens our notion that exposure to indoor-related microbe damage increases the risk for systemic subclinical inflammation and creates a health risk for building users.


Assuntos
Microbiologia do Ar , Atividade Bactericida do Sangue/imunologia , Proteínas do Sistema Complemento/imunologia , Bactérias , Carga Bacteriana , Contagem de Colônia Microbiana , Via Alternativa do Complemento , Via Clássica do Complemento , Escherichia coli , Fungos , Humanos , Valores de Referência , Síndrome do Edifício Doente/imunologia , Síndrome do Edifício Doente/microbiologia
3.
PLoS One ; 14(4): e0215179, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30964926

RESUMO

The aim of the study was to develop a method for early detection and identification of fungal contamination of building materials using an electronic nose. Therefore, the laboratory experiments based on the analysis of the air in the vicinity of fungal isolates potentially found in the building materials were performed. The results revealed that the employed gas sensors array consisting of MOS-type sensors enables the detection of the differences among the examined samples of fungi and distinguishing between the non-contaminated and contaminated samples, shortly after fungal contamination occurs. Electronic nose readouts were analysed using Principal Component Analysis and the results were verified with standard chromatographic analysis by means of SPME-GC/MS method, which proved that gas sensors array can be applied for early detection of fungal contamination.


Assuntos
Materiais de Construção/microbiologia , Nariz Eletrônico , Microbiologia Ambiental , Fungos/isolamento & purificação , Microbiologia do Ar , Poluição do Ar em Ambientes Fechados/análise , Nariz Eletrônico/estatística & dados numéricos , Fungos/química , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Análise de Componente Principal , Semicondutores , Síndrome do Edifício Doente/microbiologia , Microextração em Fase Sólida
4.
Occup Med (Lond) ; 63(4): 287-90, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23599177

RESUMO

BACKGROUND: Working in damp conditions is associated with asthma, but few studies have used objective testing to document work-related patterns. AIMS: To describe the relationship of peak flow measurements to work-related asthma (WRA) symptoms and WRA among occupants in a damp office building. METHODS: At the beginning of the study, all workers were offered a questionnaire and methacholine challenge testing. Participants were then instructed to perform serial spirometry using handheld spirometers five times per day over a 3 week period. Peak flow data were analysed using OASYS-2 software. We calculated the area between the curves (ABC score) using hours from waking. We considered a score >5.6 L/min/h to be indicative of a work-related pattern. RESULTS: All 24 employees participated in the questionnaire. Seven participants (29%) reported physician-diagnosed asthma with onset after starting work in the building. Almost two-thirds (63%) of participants reported at least one lower respiratory symptom (LRS) occurring one or more times per week in the last 4 weeks. Twenty-two (92%) consented to participate in serial spirometry. Fourteen participants had adequate quality of serial spirometry, five of whom had ABC scores >5.6, ranging from 5.9-23.0. Of these five, two had airways responsiveness, three had current post-hire onset physician-diagnosed asthma and four reported work-related LRS. CONCLUSIONS: We found evidence of work-related changes in serial peak flows among some occupants of an office building with a history of dampness. Serial peak flows may be a useful measure to determine WRA in office settings.


Assuntos
Asma Ocupacional/fisiopatologia , Exposição Ocupacional/efeitos adversos , Pico do Fluxo Expiratório/fisiologia , Síndrome do Edifício Doente/fisiopatologia , Local de Trabalho/normas , Adulto , Asma Ocupacional/diagnóstico , Asma Ocupacional/etiologia , Testes de Provocação Brônquica , Dióxido de Carbono/análise , Monitoramento Ambiental , Fungos/isolamento & purificação , Humanos , Umidade , Sensibilidade e Especificidade , Síndrome do Edifício Doente/microbiologia , Inquéritos e Questionários
5.
J Expo Sci Environ Epidemiol ; 23(4): 409-15, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22968350

RESUMO

We evaluated attenuation in linear associations between microbial exposure and respiratory symptoms occurring when individual measurements of microbial agents were used for estimating employees' exposure compared with group means. Symptoms, which improved when away from the building (building-related, BR), and measurements of culturable fungi, ergosterol, and endotoxin in floor dust were obtained between 2001 and 2007 from four cross-sectional studies on occupants of a water-damaged building. We compared odds ratios from longitudinal health effect models using individual measurements at employees' workstations with those using floor (group) means. Estimated odds for BR respiratory symptoms in group-based analyses increased by 2 to 5 times compared with those from individual-based analyses for culturable fungi and ergosterol, although they were less precise. For endotoxin, we found substantially increased and significant odds in group-based analyses, while we found no associations in individual-based analyses for various symptoms. Our study suggested that the building floor was useful in constructing exposure groups for microbial agents in this water-damaged building for epidemiologic analysis. Our study showed that group-average exposure estimation provides less attenuated associations between exposures to microbial agents and health in damp indoor environments where measurement error and intrinsic temporal variability are often large.


Assuntos
Poluição do Ar em Ambientes Fechados/efeitos adversos , Exposição Ambiental/análise , Umidade/efeitos adversos , Doenças Respiratórias/microbiologia , Síndrome do Edifício Doente/microbiologia , Poluição do Ar em Ambientes Fechados/análise , Estudos Transversais , Poeira/análise , Endotoxinas/efeitos adversos , Endotoxinas/análise , Exposição Ambiental/efeitos adversos , Fungos , Inquéritos Epidemiológicos , Humanos , Doenças Respiratórias/etiologia , Síndrome do Edifício Doente/etiologia
6.
Indoor Air ; 22(4): 339-51, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22257156

RESUMO

UNLABELLED: The role of human occupancy as a source of indoor biological aerosols is poorly understood. Size-resolved concentrations of total and biological particles in indoor air were quantified in a classroom under occupied and vacant conditions. Per-occupant emission rates were estimated through a mass-balance modeling approach, and the microbial diversity of indoor and outdoor air during occupancy was determined via rDNA gene sequence analysis. Significant increases of total particle mass and bacterial genome concentrations were observed during the occupied period compared to the vacant case. These increases varied in magnitude with the particle size and ranged from 3 to 68 times for total mass, 12-2700 times for bacterial genomes, and 1.5-5.2 times for fungal genomes. Emission rates per person-hour because of occupancy were 31 mg, 37 × 10(6) genome copies, and 7.3 × 10(6) genome copies for total particle mass, bacteria, and fungi, respectively. Of the bacterial emissions, ∼18% are from taxa that are closely associated with the human skin microbiome. This analysis provides size-resolved, per person-hour emission rates for these biological particles and illustrates the extent to which being in an occupied room results in exposure to bacteria that are associated with previous or current human occupants. PRACTICAL IMPLICATIONS: Presented here are the first size-resolved, per person emission rate estimates of bacterial and fungal genomes for a common occupied indoor space. The marked differences observed between total particle and bacterial size distributions suggest that size-dependent aerosol models that use total particles as a surrogate for microbial particles incorrectly assess the fate of and human exposure to airborne bacteria. The strong signal of human microbiota in airborne particulate matter in an occupied setting demonstrates that the aerosol route can be a source of exposure to microorganisms emitted from the skin, hair, nostrils, and mouths of other occupants.


Assuntos
Microbiologia do Ar , Poluição do Ar em Ambientes Fechados/análise , Bactérias/isolamento & purificação , DNA Bacteriano/análise , DNA Fúngico/análise , Fungos/isolamento & purificação , Bactérias/classificação , Bactérias/genética , DNA Ribossômico/análise , Exposição Ambiental/efeitos adversos , Fungos/classificação , Fungos/genética , Genômica , Humanos , Tamanho da Partícula , Material Particulado/análise , Filogenia , Densidade Demográfica , Síndrome do Edifício Doente/etiologia , Síndrome do Edifício Doente/microbiologia , Estudantes , Universidades/estatística & dados numéricos
7.
Sci Total Environ ; 414: 277-86, 2012 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-22169393

RESUMO

A Penicillium polonicum, an Aspergillus ustus and a Periconia britannica strain were isolated from water-damaged environments and the production of microbial volatile organic compounds (MVOCs) was investigated by means of headspace solid-phase microextraction followed by GC-MS analysis. The most important MVOCs produced were 2-methylisoborneol, geosmin and daucane-type sesquiterpenes for P. polonicum, 1-octen-3-ol, 3-octanone, germacrene D, δ-cadinene and other sesquiterpenes for A. ustus and the volatile mycotoxin precursor aristolochene together with valencene, α-selinene and ß-selinene for P. britannica. Different growth conditions (substrate, temperature, relative humidity) were selected, resembling indoor parameters, to investigate their influence on fungal metabolism in relation with the sick building syndrome and the results were compared with two other fungal strains previously analyzed under the same conditions. In general, the range of MVOCs and the emitted quantities were larger on malt extract agar than on wallpaper and plasterboard, but, overall, the main MVOC profile was conserved also on the two building materials tested. The influence of temperature and relative humidity on growth and metabolism is different for different fungal species, and two main patterns of behavior could be distinguished. Results show that, even at suboptimal conditions for growth, production of fungal volatiles can be significant.


Assuntos
Habitação , Fungos Mitospóricos/crescimento & desenvolvimento , Fungos Mitospóricos/metabolismo , Síndrome do Edifício Doente/metabolismo , Síndrome do Edifício Doente/microbiologia , Compostos Orgânicos Voláteis/metabolismo , Análise de Variância , Canfanos/metabolismo , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Umidade , Cetonas/metabolismo , Naftóis/metabolismo , Octanóis/metabolismo , Sesquiterpenos/metabolismo , Sesquiterpenos de Germacrano/metabolismo , Extração em Fase Sólida , Especificidade da Espécie , Temperatura
8.
Int J Biol Sci ; 7(3): 261-8, 2011 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-21448336

RESUMO

Non-infectious inhaled microbial particles can cause illness by triggering an inappropriate immunological response. From the pathogenic point of view these illnesses can be seen to be related to on one hand autoimmune diseases and on the other infectious diseases.In this review three such illnesses are discussed in some detail. Hypersensitivity pneumonitis (HP) is the best known of these illnesses and it has also been widely studied in animal models and clinically. In contrast to HP Pulmonary mycotoxicosis (PM) is not considered to involve immunological memory, it is an acute self-limiting condition is caused by an immediate "toxic" effect. Damp building related illness (DBRI) is a controversial and from a diagnostic point poorly defined entity that is however causing, or attributed to cause, much more morbidity than the two other diseases.In the recent decade there has been a shift in the focus of immunology from the lymphocyte centered, adaptive immunity towards innate immunity. The archetypal cell in innate immunity is the macrophage although many other cell types participate. Innate immunity relies on a limited number of germline coded receptors for the recognition of pathogens and signs of cellular damage. The focus on innate immunity has opened new paths for the understanding of many chronic inflammatory diseases. The purpose of this review is to discuss the impact of some recent studies, that include aspects concerning innate immunity, on our understanding of the pathogenesis of inflammatory diseases associated with exposure to inhaled microbial matter.


Assuntos
Doença Ambiental/imunologia , Imunidade Inata/fisiologia , Alveolite Alérgica Extrínseca/imunologia , Alveolite Alérgica Extrínseca/microbiologia , Animais , Doença Ambiental/microbiologia , Humanos , Macrófagos/fisiologia , Micotoxicose/imunologia , Micotoxicose/microbiologia , Síndrome do Edifício Doente/imunologia , Síndrome do Edifício Doente/microbiologia
9.
Indoor Air ; 21(3): 253-63, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21198888

RESUMO

UNLABELLED: This study investigated the possible relationships between exposures to mite allergen and airborne fungi with sick building syndrome (SBS) symptoms for residents living in newly built dwellings. We randomly sampled 5709 newly built dwellings in six prefectures from northern to southern Japan. A total of 1479 residents in 425 households participated in the study by completing questionnaire surveys and agreeing to environmental monitoring for mite allergen (Der 1), airborne fungi, aldehydes, and volatile organic compounds. Stepwise logistic regression analyses adjusted for confounders were used to obtain odds ratios (OR) of mite allergen and fungi for SBS symptoms. Der 1 had a significantly high OR for nose symptoms. Rhodotorula had a significantly high OR for any symptoms, and Aspergillus had significantly high OR for eye symptoms. However, the total colony-forming units had a significantly low OR for throat and respiratory symptoms. Eurotium had a significantly low OR for skin symptoms. In conclusion, dust-mite allergen levels and indoor airborne Rhodotorula and Aspergillus concentrations may result in SBS symptoms in newly built dwellings. PRACTICAL IMPLICATIONS: Various factors can cause sick building syndrome symptoms. This study focused on biologic factors such as dust-mite allergen and airborne fungi in newly built dwellings in Japan. Dust-mite allergen levels were significantly associated with higher rates of nose symptoms, airborne Rhodotorula concentrations were significantly associated with higher rates of any symptoms, and Aspergillus concentrations were significantly associated with higher rates of eye symptoms. Measures should be taken to reduce mite allergen levels and fungal concentrations in these dwellings.


Assuntos
Alérgenos/efeitos adversos , Antígenos de Dermatophagoides/efeitos adversos , Fungos Mitospóricos/isolamento & purificação , Síndrome do Edifício Doente/etiologia , Acetona/efeitos adversos , Acetona/análise , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Poluição do Ar em Ambientes Fechados/efeitos adversos , Poluição do Ar em Ambientes Fechados/análise , Aldeídos/efeitos adversos , Aldeídos/análise , Alérgenos/análise , Antígenos de Dermatophagoides/análise , Proteínas de Artrópodes , Criança , Pré-Escolar , Estudos Transversais , Cisteína Endopeptidases , Feminino , Habitação , Humanos , Lactente , Japão/epidemiologia , Masculino , Pessoa de Meia-Idade , Razão de Chances , Síndrome do Edifício Doente/epidemiologia , Síndrome do Edifício Doente/microbiologia , Inquéritos e Questionários , Compostos Orgânicos Voláteis/efeitos adversos , Compostos Orgânicos Voláteis/análise , Adulto Jovem
10.
Front Biosci (Elite Ed) ; 3(2): 562-80, 2011 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-21196335

RESUMO

The following is a review of some of the work that we have done since 2007 regarding the importance of molds in the phenomenon of sick building syndrome (SBS). In these studies we first examined mold contamination in air handling units (AHU). Our results showed that Cladosporium sp. were commonly recovered in AHU as growth sites and free spores. They were found mainly on the blower wheel fan blades, the ductwork, and cooling coil fans. Our results showed that the presence of species of molds other than Cladosporium in locations other than the blower wheel blades indicated that the AHU condition was not optimal. In a series of three papers, we examined growth and mycotoxin production by Chaetomium globosum (CG). In these studies we showed that CG produces two potent mycotoxins, chaetoglobosin A (Ch-A) and chaetoglobosin C (Ch-C) when grown on building material. We discovered that these toxins break down when exposed to temperatures in excess of 75 degrees C. We also showed that growth and mycotoxin production by CG is favored at a neutral pH. In another study, we showed that mycotoxins can be detected in body fluids and human tissues from patients exposed to mycotoxin producing molds, and we showed which human tissues or fluids were the most likely to give positive results for detection of these compounds. Finally, we showed that the macrocyclic trichothecene mycotoxins (MTM) produced by Stachybotrys chartarum (SC) are detectable in experimental animals soon after exposure and we described the dynamics of MTM tissue loading.


Assuntos
Ar Condicionado/efeitos adversos , Microbiologia do Ar , Poluição do Ar em Ambientes Fechados , Exposição Ambiental , Fungos/crescimento & desenvolvimento , Síndrome do Edifício Doente/microbiologia , Fungos/química , Fungos/isolamento & purificação , Humanos , Concentração de Íons de Hidrogênio , Alcaloides Indólicos/toxicidade , Micotoxinas/toxicidade , Temperatura
11.
Gig Sanit ; (5): 26-8, 2010.
Artigo em Russo | MEDLINE | ID: mdl-21344696

RESUMO

Dwelling and public buildings underwent comprehensive hygienic studies for fungal contamination. Human allergization associated with fungal contamination within the building envelopes and with the viable fungal spores in the air of enclosed spaces was found to be prevalent. The leading factors determining the extent to which the internal environment of premises was exposed to fungal contamination: their increased air humidity due to leakages and inlets, the affected area of building envelopes, and a temperature factor were revealed. The criteria showing it necessary to undertake specific measures to optimize the living conditions of the population were defined.


Assuntos
Microbiologia do Ar , Poluição do Ar em Ambientes Fechados/análise , Monitoramento Ambiental/métodos , Arquitetura de Instituições de Saúde , Higiene , Síndrome do Edifício Doente/microbiologia , Esporos Fúngicos/isolamento & purificação , Humanos , Síndrome do Edifício Doente/prevenção & controle
12.
Crit Rev Toxicol ; 39(10): 799-864, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19863384

RESUMO

Fungal spores are ubiquitous in the environment. However, exposure levels in workplaces where mouldy materials are handled are much higher than in common indoor and outdoor environments. Spores of all tested species induced inflammation in experimental studies. The response to mycotoxin-producing and pathogenic species was much stronger. In animal studies, nonallergic responses dominated after a single dose. Allergic responses also occurred, especially to mycotoxin-producing and pathogenic species, and after repeated exposures. Inhalation of a single spore dose by subjects with sick building syndrome indicated no observed effect levels of 4 x 10(3) Trichoderma harzianum spores/m(3) and 8 x 10(3) Penicillium chrysogenum spores/m(3) for lung function, respiratory symptoms, and inflammatory cells in the blood. In asthmatic patients allergic to Penicillium sp. or Alternaria alternata, lowest observed effect levels (LOELs) for reduced airway conductance were 1 x 10(4) and 2 x 10(4) spores/m(3), respectively. In epidemiological studies of highly exposed working populations lung function decline, respiratory symptoms and airway inflammation began to appear at exposure levels of 10(5) spores/m(3). Thus, human challenge and epidemiological studies support fairly consistent LOELs of approximately 10(5) spores/m(3) for diverse fungal species in nonsensitised populations. Mycotoxin-producing and pathogenic species have to be detected specifically, however, because of their higher toxicity.


Assuntos
Exposição por Inalação/efeitos adversos , Exposição Ocupacional/efeitos adversos , Esporos Fúngicos/isolamento & purificação , Poluentes Ocupacionais do Ar/imunologia , Poluentes Ocupacionais do Ar/toxicidade , Animais , Monitoramento Ambiental/métodos , Humanos , Inflamação/imunologia , Inflamação/microbiologia , Exposição por Inalação/prevenção & controle , Concentração Máxima Permitida , Exposição Ocupacional/prevenção & controle , Síndrome do Edifício Doente/microbiologia , Síndrome do Edifício Doente/prevenção & controle , Esporos Fúngicos/imunologia
13.
Toxicol Ind Health ; 25(9-10): 571-6, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19808743

RESUMO

Molds are ubiquitous throughout the biosphere of planet earth and cause infectious, allergic, and toxic diseases. Toxic diseases arise from exposure to mycotoxins produced by molds. Throughout history, there have been a number of toxic epidemics associated with exposure to mycotoxins. Acute epidemics of ergotism are caused by consumption of grain infested by fungi of the genus Claviceps, which produce the bioactive amine ergotamine that mimics the neurotransmitters norepinephrine, serotonin, and dopamine. Acute aflatoxin outbreaks have occurred from ingestion of corn stored in damp conditions that potentiate growth of the molds of the species Aspergillus. Contemporary construction methods that use cellulose substrates such as fiber board and indoor moisture have caused an outbreak of contaminated buildings with Stachybotrys chartarum, with the extent of health effects still a subject of debate and ongoing research. This article reviews several of the more prominent epidemics and discusses the nature of the toxins. Two diseases that were leading causes of childhood mortality in England in the 1970s and vanished with changing dietary habits, putrid malignant fever, and slow nervous fever were most likely toxic mold epidemics.


Assuntos
Surtos de Doenças , Fungos/fisiologia , Fungos/patogenicidade , Micoses/epidemiologia , Doença Aguda , Aspergilose/epidemiologia , Aspergilose/microbiologia , Materiais de Construção/microbiologia , Ergotismo/epidemiologia , Ergotismo/microbiologia , Fungos/isolamento & purificação , Habitação , Humanos , Micoses/microbiologia , Síndrome do Edifício Doente/epidemiologia , Síndrome do Edifício Doente/microbiologia
14.
Toxicol Ind Health ; 25(9-10): 617-35, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19854820

RESUMO

The following is a review of some of the work we have done since 2004 regarding the importance of molds and their mycotoxins in the phenomenon of sick building syndrome (SBS). In these studies we showed that the macrocyclic trichothecene mycotoxins (MTM) of Stachybotrys chartarum (SC) are easily dissociated from the surface of the organism as it grows and could therefore be consequently spread in buildings as the fungus experiences additional water events. We then showed that SC and Penicillium chrysogenum (PC) colonies remain viable long after a water source has been removed, and the MTM produced by SC remain toxic over extended periods of time. We next showed that PC when inhaled, can release in vivo, a protease allergen that can cause a significant allergic inflammatory reaction in the lungs of mice. We then showed, in a laboratory study, that the MTM of SC can become airborne attached to spores or SC particulates smaller than spores. Following that study, we next showed that the same phenomenon actually occurred in SC infested buildings where people were complaining of health problems potentially associated with SBS. Finally, we were able to demonstrate the presence of MTM in the sera of individuals who had been exposed to SC in indoor environments. This last study was done with enough mold exposed individuals to allow for the statistical significance of SC exposure to be evaluated.


Assuntos
Poluição do Ar em Ambientes Fechados/efeitos adversos , Micotoxinas/efeitos adversos , Penicillium chrysogenum/patogenicidade , Síndrome do Edifício Doente/microbiologia , Stachybotrys/patogenicidade , Animais , Modelos Animais de Doenças , Humanos , Exposição por Inalação , Camundongos , Penicillium chrysogenum/isolamento & purificação , Penicillium chrysogenum/fisiologia , Stachybotrys/isolamento & purificação , Stachybotrys/fisiologia , Microbiologia da Água
15.
J Med Invest ; 56(3-4): 150-6, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19763028

RESUMO

We previously developed a high powered light-emitting diode device capable of discharging germicidal ultraviolet irradiation (UVA-LED) at an approximate wavelength of 365 nm. This study examined the bactericidal activity of UVA-LED in moving air streams. Aerosols of Escherichia coli DH5alpha were exposed to UVA-LED irradiation using a stable current (0.5 A and 1.2 mW/cm(2)) or pulse current (1.0 A and 0.2 mW/cm(2)). Settle plate analysis was used for bioaerosol sampling, where results were expressed as Colony Forming Units. A -3 Log inactivation of the E. coli population occurred after 75 minutes of constant exposure to stable current. The pulse current produced inactivation within a similar timeframe. Our results might be significant as a basic study for further investigations about the effect of UVA-LED on airborne bacteria and its suitability for air disinfection applications.


Assuntos
Microbiologia do Ar , Desinfecção/instrumentação , Raios Ultravioleta , Aerossóis , Poluição do Ar em Ambientes Fechados , Escherichia coli/efeitos da radiação , Humanos , Síndrome do Edifício Doente/microbiologia , Síndrome do Edifício Doente/prevenção & controle
17.
Inhal Toxicol ; 21(10): 857-67, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19459771

RESUMO

Oxidative stress has been proposed to be one mechanism behind the adverse health outcomes associated with living in a damp indoor environment. In the present study, the capability of damp building-related microbes Streptomyces californicus and Stachybotrys chartarum to induce oxidative stress was evaluated in vitro. In addition, the role of oxidative stress in provoking the detected cytotoxic, genotoxic, and inflammatory responses was studied by inhibiting the production of reactive oxygen species (ROS) using N-acetyl-l-cysteine (NAC). RAW264.7 macrophages were exposed in a dose- and time-dependent manner to the spores of co-cultivated S. californicus and S. chartarum, to their separately cultivated spore-mixture, or to the spores of these microbes alone. The intracellular peroxide production and cytotoxicity were measured by flow cytometric analysis, nitric oxide production was analyzed by the Griess method, DNA damage was determined by the comet assay, and cytokine production was measured by an immunochemical ELISA (enzyme-linked immunosorbent assay). All the studied microbial exposures triggered oxidative stress and subsequent cellular damage in RAW264.7 macrophages. The ROS scavenger, NAC, prevented growth arrest, apoptosis, DNA damage, and cytokine production induced by the co-culture since it reduced the intracellular level of ROS within macrophages. In contrast, the DNA damage and cell cycle arrest induced by the spores of S. californicus alone could not be prevented by NAC. Bioaerosol-induced oxidative stress in macrophages may be an important mechanism behind the frequent respiratory symptoms and diseases suffered by residents of moisture damaged buildings. Furthermore, microbial interactions during co-cultivation stimulate the production of highly toxic compound(s) which may significantly increase oxidative damage.


Assuntos
Imunotoxinas/toxicidade , Mutagênicos/toxicidade , Estresse Oxidativo/efeitos dos fármacos , Síndrome do Edifício Doente/microbiologia , Stachybotrys/metabolismo , Streptomyces/metabolismo , Acetilcisteína/metabolismo , Animais , Ciclo Celular/efeitos dos fármacos , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Ensaio Cometa , Citocinas/biossíntese , DNA/biossíntese , DNA/genética , Cães , Relação Dose-Resposta a Droga , Citometria de Fluxo , Humanos , Peroxidação de Lipídeos/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Macrófagos/imunologia , Espécies Reativas de Oxigênio/metabolismo , Esporos Bacterianos/química , Esporos Bacterianos/metabolismo , Stachybotrys/imunologia , Streptomyces/imunologia
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