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1.
Biomolecules ; 13(12)2023 12 05.
Artigo em Inglês | MEDLINE | ID: mdl-38136617

RESUMO

Menkes' disease (MD) and Wilson's disease (WD) are two major copper (Cu) metabolism-related disorders caused by mutations of the ATP7A and ATP7B ATPase gene, respectively. While Cu is involved in DNA strand breaks signaling and repair, the response of cells from both diseases to ionizing radiation, a common DNA strand breaks inducer, has not been investigated yet. To this aim, three MD and two WD skin fibroblasts lines were irradiated at two Gy X-rays and clonogenic cell survival, micronuclei, anti-γH2AX, -pATM, and -MRE11 immunofluorescence assays were applied to evaluate the DNA double-strand breaks (DSB) recognition and repair. MD and WD cells appeared moderately radiosensitive with a delay in the radiation-induced ATM nucleo-shuttling (RIANS) associated with impairments in the DSB recognition. Such delayed RIANS was notably caused in both MD and WD cells by a highly expressed ATP7B protein that forms complexes with ATM monomers in cytoplasm. Interestingly, a Cu pre-treatment of cells may influence the activity of the MRE11 nuclease and modulate the radiobiological phenotype. Lastly, some high-passage MD cells cultured in routine may transform spontaneously becoming immortalized. Altogether, our findings suggest that exposure to ionizing radiation may impact on clinical features of MD and WD, which requires cautiousness when affected patients are submitted to radiodiagnosis and, eventually, radiotherapy.


Assuntos
Degeneração Hepatolenticular , Síndrome dos Cabelos Torcidos , Humanos , Cobre/metabolismo , Proteínas Quinases/metabolismo , Radiação Ionizante , Síndrome dos Cabelos Torcidos/genética , Síndrome dos Cabelos Torcidos/metabolismo , Degeneração Hepatolenticular/genética , Fibroblastos/metabolismo , DNA/metabolismo , Proteínas Mutadas de Ataxia Telangiectasia/metabolismo
2.
PLoS Genet ; 19(1): e1010558, 2023 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-36626371

RESUMO

Copper (Cu) has a multifaceted role in brain development, function, and metabolism. Two homologous Cu transporters, Atp7a (Menkes disease protein) and Atp7b (Wilson disease protein), maintain Cu homeostasis in the tissue. Atp7a mediates Cu entry into the brain and activates Cu-dependent enzymes, whereas the role of Atp7b is less clear. We show that during postnatal development Atp7b is necessary for normal morphology and function of choroid plexus (ChPl). Inactivation of Atp7b causes reorganization of ChPl' cytoskeleton and cell-cell contacts, loss of Slc31a1 from the apical membrane, and a decrease in the length and number of microvilli and cilia. In ChPl lacking Atp7b, Atp7a is upregulated but remains intracellular, which limits Cu transport into the brain and results in significant Cu deficit, which is reversed only in older animals. Cu deficiency is associated with down-regulation of Atp7a in locus coeruleus and catecholamine imbalance, despite normal expression of dopamine-ß-hydroxylase. In addition, there are notable changes in the brain lipidome, which can be attributed to inhibition of diacylglyceride-to-phosphatidylethanolamine conversion. These results identify the new role for Atp7b in developing brain and identify metabolic changes that could be exacerbated by Cu chelation therapy.


Assuntos
Cobre , Síndrome dos Cabelos Torcidos , Camundongos , Animais , ATPases Transportadoras de Cobre , Cobre/metabolismo , Plexo Corióideo/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Encéfalo/metabolismo
3.
Int J Mol Sci ; 23(19)2022 Sep 28.
Artigo em Inglês | MEDLINE | ID: mdl-36232742

RESUMO

Kidneys play an especial role in copper redistribution in the organism. The epithelial cells of proximal tubules perform the functions of both copper uptake from the primary urine and release to the blood. These cells are equipped on their apical and basal membrane with copper transporters CTR1 and ATP7A. Mosaic mutant mice displaying a functional dysfunction of ATP7A are an established model of Menkes disease. These mice exhibit systemic copper deficiency despite renal copper overload, enhanced by copper therapy, which is indispensable for their life span extension. The aim of this study was to analyze the expression of Slc31a1 and Slc31a2 genes (encoding CTR1/CTR2 proteins) and the cellular localization of the CTR1 protein in suckling, young and adult mosaic mutants. Our results indicate that in the kidney of both intact and copper-injected 14-day-old mutants showing high renal copper content, CTR1 mRNA level is not up-regulated compared to wild-type mice given a copper injection. The expression of the Slc31a1 gene in 45-day-old mice is even reduced compared with intact wild-type animals. In suckling and young copper-injected mutants, the CTR1 protein is relocalized from the apical membrane to the cytoplasm of epithelial cells of proximal tubules, the process which prevents copper transport from the primary urine and, thus, protects cells against copper toxicity.


Assuntos
Transportador de Cobre 1 , Cobre , Células Epiteliais , Túbulos Renais Proximais , Síndrome dos Cabelos Torcidos , Animais , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Membrana Celular/genética , Membrana Celular/metabolismo , Cobre/metabolismo , Cobre/toxicidade , Transportador de Cobre 1/genética , Transportador de Cobre 1/metabolismo , ATPases Transportadoras de Cobre/genética , ATPases Transportadoras de Cobre/metabolismo , Citoplasma/genética , Citoplasma/metabolismo , Modelos Animais de Doenças , Células Epiteliais/metabolismo , Expressão Gênica , Túbulos Renais Proximais/metabolismo , Proteínas de Membrana/genética , Proteínas de Membrana/metabolismo , Síndrome dos Cabelos Torcidos/etiologia , Síndrome dos Cabelos Torcidos/genética , Síndrome dos Cabelos Torcidos/metabolismo , Camundongos , Transporte Proteico/genética , Transporte Proteico/fisiologia , RNA Mensageiro/metabolismo , Proteínas SLC31/genética , Proteínas SLC31/metabolismo
4.
Pflugers Arch ; 472(10): 1415-1429, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-32506322

RESUMO

Copper is an essential element in cells; it can act as either a recipient or a donor of electrons, participating in various reactions. However, an excess of copper ions in cells is detrimental as these copper ions can generate free radicals and increase oxidative stress. In multicellular organisms, copper metabolism involves uptake, distribution, sequestration, and excretion, at both the cellular and systemic levels. Mammalian enterocytes take in bioavailable copper ions from the diet in a Ctr1-dependent manner. After incorporation, cuprous ions are delivered to ATP7A, which pumps Cu+ from enterocytes into the blood. Copper ions arrive at the liver through the portal vein and are incorporated into hepatocytes by Ctr1. Then, Cu+ can be secreted into the bile or the blood via the Atox1/ATP7B/ceruloplasmin route. In the bloodstream, this micronutrient can reach peripheral tissues and is again incorporated by Ctr1. In peripheral tissue cells, cuprous ions are either sequestrated by molecules such as metallothioneins or targeted to utilization pathways by chaperons such as Atox1, Cox17, and CCS. Copper metabolism must be tightly controlled in order to achieve homeostasis and avoid disorders. A hereditary or acquired copper unbalance, including deficiency, overload, or misdistribution, may cause or aggravate certain diseases such as Menkes disease, Wilson disease, neurodegenerative diseases, anemia, metabolic syndrome, cardiovascular diseases, and cancer. A full understanding of copper metabolism and its roles in diseases underlies the identification of novel effective therapies for such diseases.


Assuntos
Cobre/metabolismo , Degeneração Hepatolenticular/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Animais , Cobre/deficiência , ATPases Transportadoras de Cobre/genética , ATPases Transportadoras de Cobre/metabolismo , Degeneração Hepatolenticular/genética , Humanos , Síndrome dos Cabelos Torcidos/genética , Chaperonas Moleculares/genética , Chaperonas Moleculares/metabolismo
5.
Science ; 368(6491): 620-625, 2020 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-32381719

RESUMO

Loss-of-function mutations in the copper (Cu) transporter ATP7A cause Menkes disease. Menkes is an infantile, fatal, hereditary copper-deficiency disorder that is characterized by progressive neurological injury culminating in death, typically by 3 years of age. Severe copper deficiency leads to multiple pathologies, including impaired energy generation caused by cytochrome c oxidase dysfunction in the mitochondria. Here we report that the small molecule elesclomol escorted copper to the mitochondria and increased cytochrome c oxidase levels in the brain. Through this mechanism, elesclomol prevented detrimental neurodegenerative changes and improved the survival of the mottled-brindled mouse-a murine model of severe Menkes disease. Thus, elesclomol holds promise for the treatment of Menkes and associated disorders of hereditary copper deficiency.


Assuntos
Cobre/metabolismo , Hidrazinas/uso terapêutico , Síndrome dos Cabelos Torcidos/tratamento farmacológico , Animais , Transporte Biológico/efeitos dos fármacos , Encéfalo/metabolismo , Encéfalo/patologia , Linhagem Celular , Transportador de Cobre 1/genética , Modelos Animais de Doenças , Complexo IV da Cadeia de Transporte de Elétrons/metabolismo , Hidrazinas/farmacologia , Masculino , Síndrome dos Cabelos Torcidos/metabolismo , Síndrome dos Cabelos Torcidos/patologia , Camundongos , Camundongos Knockout , Mitocôndrias/metabolismo , Doenças Neurodegenerativas/prevenção & controle , Ratos
6.
Proc Natl Acad Sci U S A ; 116(25): 12167-12172, 2019 06 18.
Artigo em Inglês | MEDLINE | ID: mdl-31160463

RESUMO

Copper is controlled by a sophisticated network of transport and storage proteins within mammalian cells, yet its uptake and efflux occur with rapid kinetics. Present as Cu(I) within the reducing intracellular environment, the nature of this labile copper pool remains elusive. While glutathione is involved in copper homeostasis and has been assumed to buffer intracellular copper, we demonstrate with a ratiometric fluorescent indicator, crisp-17, that cytosolic Cu(I) levels are buffered to the vicinity of 1 aM, where negligible complexation by glutathione is expected. Enabled by our phosphine sulfide-stabilized phosphine (PSP) ligand design strategy, crisp-17 offers a Cu(I) dissociation constant of 8 aM, thus exceeding the binding affinities of previous synthetic Cu(I) probes by four to six orders of magnitude. Two-photon excitation microscopy with crisp-17 revealed rapid, reversible increases in intracellular Cu(I) availability upon addition of the ionophoric complex CuGTSM or the thiol-selective oxidant 2,2'-dithiodipyridine (DTDP). While the latter effect was dramatically enhanced in 3T3 cells grown in the presence of supplemental copper and in cultured Menkes mutant fibroblasts exhibiting impaired copper efflux, basal Cu(I) availability in these cells showed little difference from controls, despite large increases in total copper content. Intracellular copper is thus tightly buffered by endogenous thiol ligands with significantly higher affinity than glutathione. The dual utility of crisp-17 to detect normal intracellular buffered Cu(I) levels as well as to probe the depth of the labile copper pool in conjunction with DTDP provides a promising strategy to characterize perturbations of cellular copper homeostasis.


Assuntos
Cobre/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Soluções Tampão , Fibroblastos/metabolismo , Corantes Fluorescentes , Glutationa/metabolismo , Ligantes , Microscopia de Fluorescência por Excitação Multifotônica/métodos , Mutação , Fosfinas/metabolismo
7.
J Inherit Metab Dis ; 41(6): 1285-1291, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-30132231

RESUMO

INTRODUCTION: Menkes disease (MD) is an X-linked recessive disorder caused by dysfunction of a copper-transporting protein, leading to severe neurodegeneration in early childhood. We investigated whether a lipophilic copper chelator, disulfiram, could enhance copper absorption from the intestine and transport copper across the blood-brain barrier in MD model mice. METHODS: Wild type and MD model mice were pretreated with disulfiram for 30 min before oral administration of 64CuCl2. Each organ was sequentially analyzed for radioactivity with γ counting. Copper uptake into the brain parenchyma was assessed by ex vivo autoradiography. RESULTS: In wild type mice, orally administered copper was initially detected in the intestine within 2 h, reaching a maximum level in the liver (19.6 ± 3.8 percentage injected dose per gram [%ID/g]) at 6 h. In MD model mice, the copper reached the maximum level in the liver (5.3 ± 1.5 %ID/g) at 4 h, which was lower than that of wild type mice (19.0 ± 7.4 %ID/g) (P < 0.05). Pretreatment of disulfiram in MD model mice increased the copper level in the brain (0.59 ± 0.28 %ID/g) at 24 h compared with MD model mice without disulfiram (0.07 ± 0.05 %ID/g) (P < 0.05). Ex vivo autoradiography revealed that high levels of copper uptake was observed in the cerebral cortex upon disulfiram pretreatment. CONCLUSION: Our data demonstrated that disulfiram enhanced the delivery of orally administered copper into the central nervous system in MD model mice. The administration of disulfiram will enable patients to avoid unpleasant subcutaneous copper injection in the future.


Assuntos
Cobre/farmacologia , Dissulfiram/uso terapêutico , Portadores de Fármacos , Síndrome dos Cabelos Torcidos/tratamento farmacológico , Animais , Autorradiografia , Barreira Hematoencefálica/metabolismo , Cobre/metabolismo , Modelos Animais de Doenças , Masculino , Síndrome dos Cabelos Torcidos/sangue , Síndrome dos Cabelos Torcidos/metabolismo , Camundongos , Camundongos Endogâmicos C3H
8.
Metallomics ; 10(3): 474-485, 2018 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-29507920

RESUMO

Copper is essential for eukaryotic life, and animals must acquire this nutrient through the diet and distribute it to cells and organelles for proper function of biological targets. Indeed, mutations in the central copper exporter ATP7A contribute to a spectrum of diseases, including Menkes disease, with symptoms ranging from neurodegeneration to lax connective tissue. As such, a better understanding of the fundamental impacts of ATP7A mutations on in vivo copper distributions is of relevance to those affected by these diseases. Here we combine metal imaging and optical imaging techniques at a variety of spatial resolutions to identify tissues and structures with altered copper levels in the Calamitygw71 zebrafish model of Menkes disease. Rapid profiling of tissue slices with LA-ICP-MS identified reduced copper levels in the brain, neuroretina, and liver of Menkes fish compared to control specimens. High resolution nanoSIMS imaging of the neuroretina, combined with electron and confocal microscopies, identified the megamitochondria of photoreceptors as loci of copper accumulation in wildtype fish, with lower levels of megamitochondrial copper observed in Calamitygw71 zebrafish. Interestingly, this localized copper decrease does not result in impaired photoreceptor development or altered megamitochondrial morphology, suggesting the prioritization of copper at sufficient levels for maintaining essential mitochondrial functions. Together, these data establish the Calamitygw71 zebrafish as an optically transparent in vivo model for the study of neural copper misregulation, illuminate a role for the ATP7A copper exporter in trafficking copper to the neuroretina, and highlight the utility of combining multiple imaging techniques for studying metals in whole organism settings with spatial resolution.


Assuntos
Cobre/metabolismo , Modelos Animais de Doenças , Síndrome dos Cabelos Torcidos/metabolismo , Mitocôndrias/metabolismo , Células Fotorreceptoras de Vertebrados/metabolismo , Peixe-Zebra/metabolismo , Animais , ATPases Transportadoras de Cobre/genética , ATPases Transportadoras de Cobre/metabolismo , Terapia a Laser , Síndrome dos Cabelos Torcidos/patologia , Imagem Multimodal/métodos , Mutação , Nanotecnologia , Fenótipo , Células Fotorreceptoras de Vertebrados/patologia , Espectrometria de Massas por Ionização por Electrospray , Espectrometria de Massa de Íon Secundário , Peixe-Zebra/crescimento & desenvolvimento
9.
Arch Dis Child Educ Pract Ed ; 102(6): 319-327, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28751535

RESUMO

In paediatrics, one of our main aims in the diagnostic process is to identify any treatable conditions. The copper metabolism disorder Wilson's disease (WD) is one such condition that is caused by mutations in the ATP7B gene. Delay in treatment could result in irreversible disability or even death. Although liver disease is the most common presenting feature in children, some children may initially present with a subtle neurological presentation only. In patients presenting with dystonia, tremor, dysarthria or with a deterioration in school performance, there should be a high index of suspicion for WD. However, the differential of these clinical presentations is wide and exclusion of WD is difficult. No single diagnostic test can exclude WD and each of the biochemical tests has limitations. In this article, we discuss copper metabolism disorders including WD and Menke's disease. We then discuss the available diagnostic tests and how to investigate children for these rare disorders.


Assuntos
Degeneração Hepatolenticular/diagnóstico , Síndrome dos Cabelos Torcidos/diagnóstico , Adolescente , Algoritmos , Proteína C-Reativa/metabolismo , Ceruloplasmina/metabolismo , Criança , Pré-Escolar , Cobre/metabolismo , Feminino , Testes Genéticos , Degeneração Hepatolenticular/complicações , Degeneração Hepatolenticular/metabolismo , Humanos , Lactente , Testes de Função Hepática , Masculino , Síndrome dos Cabelos Torcidos/complicações , Síndrome dos Cabelos Torcidos/metabolismo , Adulto Jovem
10.
Sci Rep ; 7(1): 757, 2017 04 07.
Artigo em Inglês | MEDLINE | ID: mdl-28389643

RESUMO

Menkes disease (MD) is caused by mutations in ATP7A, encoding a copper-transporting P-type ATPase which exhibits copper-dependent trafficking. ATP7A is found in the Trans-Golgi Network (TGN) at low copper concentrations, and in the post-Golgi compartments and the plasma membrane at higher concentrations. Here we have analyzed the effect of 36 ATP7A missense mutations identified in phenotypically different MD patients. Nine mutations identified in patients with severe MD, virtually eliminated ATP7A synthesis, in most cases due to aberrant RNA splicing. A group of 21 predominantly severe mutations led to trapping of the protein in TGN and displayed essentially no activity in a yeast-based functional assay. These were predicted to inhibit the catalytic phosphorylation of the protein. Four mutants showed diffuse post-TGN localization, while two displayed copper dependent trafficking. These six variants were identified in patients with mild MD and typically displayed activity in the yeast assay. The four post-TGN located mutants were presumably affected in the catalytic dephosphorylation of the protein. Together these results indicate that the severity of MD correlate with cellular localization of ATP7A and support previous studies indicating that phosphorylation is crucial for the exit of ATP7A from TGN, while dephosphorylation is crucial for recycling back to TGN.


Assuntos
ATPases Transportadoras de Cobre/genética , ATPases Transportadoras de Cobre/metabolismo , Síndrome dos Cabelos Torcidos/genética , Síndrome dos Cabelos Torcidos/metabolismo , Mutação de Sentido Incorreto , Alelos , Processamento Alternativo , Substituição de Aminoácidos , Biomarcadores , Cobre/metabolismo , Fibroblastos , Imunofluorescência , Humanos , Espaço Intracelular/metabolismo , Síndrome dos Cabelos Torcidos/diagnóstico , Modelos Biológicos , Fenótipo , Fosforilação , Complexo de Endopeptidases do Proteassoma , Transporte Proteico , Proteólise , Índice de Gravidade de Doença
11.
IUBMB Life ; 69(4): 263-270, 2017 04.
Artigo em Inglês | MEDLINE | ID: mdl-28271632

RESUMO

The essential transition metal copper is important in lipid metabolism, redox balance, iron mobilization, and many other critical processes in eukaryotic organisms. Genetic diseases where copper homeostasis is disrupted, including Menkes disease and Wilson disease, indicate the importance of copper balance to human health. The severe consequences of insufficient copper supply are illustrated by Menkes disease, caused by mutation in the X-linked ATP7A gene encoding a protein that transports copper from intestinal epithelia into the bloodstream and across the blood-brain barrier. Inadequate copper supply to the body due to poor diet quality or malabsorption can disrupt several molecular level pathways and processes. Though much of the copper distribution machinery has been described and consequences of disrupted copper handling have been characterized in human disease as well as animal models, physiological consequences of sub-optimal copper due to poor nutrition or malabsorption have not been extensively studied. Recent work indicates that insufficient copper may be important in a number of common diseases including obesity, ischemic heart disease, and metabolic syndrome. Specifically, marginal copper deficiency (CuD) has been reported as a potential etiologic factor in diseases characterized by disrupted lipid metabolism such as non-alcoholic fatty-liver disease (NAFLD). In this review, we discuss the available data suggesting that a significant portion of the North American population may consume insufficient copper, the potential mechanisms by which CuD may promote lipid biosynthesis, and the interaction between CuD and dietary fructose in the etiology of NAFLD. © 2016 IUBMB Life, 69(4):263-270, 2017.


Assuntos
Cobre/metabolismo , Metabolismo dos Lipídeos/genética , Lipídeos/biossíntese , Hepatopatia Gordurosa não Alcoólica/metabolismo , Adenosina Trifosfatases/genética , Adenosina Trifosfatases/metabolismo , Barreira Hematoencefálica/metabolismo , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , ATPases Transportadoras de Cobre , Dieta , Humanos , Ferro/metabolismo , Lipídeos/genética , Fígado/metabolismo , Fígado/patologia , Síndrome dos Cabelos Torcidos/genética , Síndrome dos Cabelos Torcidos/metabolismo , Hepatopatia Gordurosa não Alcoólica/dietoterapia , Hepatopatia Gordurosa não Alcoólica/genética , Hepatopatia Gordurosa não Alcoólica/patologia
12.
Folia Biol (Praha) ; 63(5-6): 165-173, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-29687769

RESUMO

Menkes disease is a severe X-linked recessive disorder caused by a defect in the ATP7A gene, which encodes a membrane copper-transporting ATPase. Deficient activity of the ATP7A protein results in decreased intestinal absorption of copper, low copper level in serum and defective distribution of copper in tissues. The clinical symptoms are caused by decreased activities of copper-dependent enzymes and include neurodegeneration, connective tissue disorders, arterial changes and hair abnormalities. Without therapy, the disease is fatal in early infancy. Rapid diagnosis of Menkes disease and early start of copper therapy is critical for the effectiveness of treatment. We report a molecular biology-based strategy that allows early diagnosis of copper transport defects and implementation of individual therapies before the full development of pathological symptoms. Low serum copper and decreased activity of copperdependent mitochondrial cytochrome c oxidase in isolated platelets found in three patients indicated a possibility of functional defects in copper-transporting proteins, especially in the ATPA7 protein, a copper- transporting P-type ATPase. Rapid mutational screening of the ATP7A gene using high-resolution melting analysis of DNA indicated presence of mutations in the patients. Molecular investigation for mutations in the ATP7A gene revealed three nonsense mutations: c.2170C>T (p.Gln724Ter); c.3745G>T (p.Glu1249Ter); and c.3862C>T (p.Gln1288Ter). The mutation c.3745G>T (p.Glu1249Ter) has not been identified previously. Molecular analysis of the ATOX1 gene as a possible modulating factor of Menkes disease did not reveal presence of pathogenic mutations. Molecular diagnostics allowed early onset of individual therapies, adequate genetic counselling and prenatal diagnosis in the affected families.


Assuntos
Proteínas de Transporte/metabolismo , ATPases Transportadoras de Cobre/metabolismo , Cobre/sangue , Síndrome dos Cabelos Torcidos/metabolismo , Mutação/genética , Proteínas de Transporte/genética , Criança , Cobre/metabolismo , Proteínas de Transporte de Cobre , ATPases Transportadoras de Cobre/genética , Humanos , Lactente , Masculino , Síndrome dos Cabelos Torcidos/genética , Metalochaperonas/genética , Metalochaperonas/metabolismo , Modelos Biológicos , Chaperonas Moleculares
13.
Sci Rep ; 6: 33247, 2016 09 15.
Artigo em Inglês | MEDLINE | ID: mdl-27629586

RESUMO

Copper (Cu) is an indispensable metal for normal development and function of humans, especially in central nervous system (CNS). However, its redox activity requires accurate Cu transport system. ATP7A, a main Cu(2+) transporting-ATPase, is necessary to efflux Cu across the plasma membrane and synthesize cuproenzymes. Menkes disease (MD) is caused by mutations in ATP7A gene. Clinically, MD is Cu deficiency syndrome and is treated with Cu-histidine injections soon after definite diagnosis. But outcome of the most remains poor. To estimate the standard therapy, Cu distribution in the treated classic MD patients is analyzed by synchrotron-generated X-ray fluorescence technique (SR-XRF), which identifies and quantifies an individual atom up to at subcellular level of resolution with wide detection area. SR-XRF analysis newly reveals that Cu exists in spinal cord parenchyma and flows out via venous and lymph systems. By systemic analysis, excess Cu is detected in the proximal tubular cells of the kidney, the mucosal epithelial cells of the intestine, and the lymph and venous systems. The current study suggests that the standard therapy supply almost enough Cu for patient tissues. But given Cu passes through the tissues to venous and lymph systems, or accumulate in the cells responsible for Cu absorption.


Assuntos
Sistema Nervoso Central/metabolismo , Cobre/metabolismo , Síndrome dos Cabelos Torcidos/diagnóstico por imagem , Síndrome dos Cabelos Torcidos/metabolismo , Sistema Nervoso Central/diagnóstico por imagem , Sistema Nervoso Central/patologia , Cobre/deficiência , ATPases Transportadoras de Cobre/sangue , ATPases Transportadoras de Cobre/genética , Fluorescência , Histidina/metabolismo , Humanos , Rim/metabolismo , Síndrome dos Cabelos Torcidos/patologia , Mutação , Radiografia , Síncrotrons , Raios X
14.
Nihon Rinsho ; 74(7): 1151-5, 2016 Jul.
Artigo em Japonês | MEDLINE | ID: mdl-27455805

RESUMO

Copper is one of essential trace elements. Copper deficiency lead to growth and developmental failure and/or neurological dysfunction. However, excess copper is also problems for human life. There are two disorders of inborn error of copper metabolism, Menkes disease and Wilson disease. Menkes disease is an X linked recessive disorder with copper deficiency and Wilson disease is an autosomal recessive disorder with copper accumulation. These both disorders result from the defective functioning of copper transport P-type ATPase, ATP7A of Menkes disease and ATP7B of Wilson disease. In this paper, the author describes about copper metabolism of human, and clinical feature, diagnosis and treatment of Menkes disease and Wilson disease.


Assuntos
Cobre/metabolismo , Predisposição Genética para Doença , Degeneração Hepatolenticular/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Degeneração Hepatolenticular/diagnóstico , Degeneração Hepatolenticular/tratamento farmacológico , Degeneração Hepatolenticular/genética , Humanos , Síndrome dos Cabelos Torcidos/diagnóstico , Síndrome dos Cabelos Torcidos/tratamento farmacológico , Síndrome dos Cabelos Torcidos/genética , Prognóstico
17.
Stem Cell Res Ther ; 6: 160, 2015 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-26347346

RESUMO

INTRODUCTION: Bone abnormalities, one of the primary manifestations of Menkes disease (MD), include a weakened bone matrix and low mineral density. However, the molecular and cellular mechanisms underlying these bone defects are poorly understood. METHODS: We present in vitro modeling for impaired osteogenesis in MD using human induced pluripotent stem cells (iPSCs) with a mutated ATP7A gene. MD-iPSC lines were generated from two patients harboring different mutations. RESULTS: The MD-iPSCs showed a remarkable retardation in CD105 expression with morphological anomalies during development to mesenchymal stem cells (MSCs) compared with wild-type (WT)-iPSCs. Interestingly, although prolonged culture enhanced CD105 expression, mature MD-MSCs presented with low alkaline phosphatase activity, reduced calcium deposition in the extracellular matrix, and downregulated osteoblast-specific genes during osteoblast differentiation in vitro. Knockdown of ATP7A also impaired osteogenesis in WT-MSCs. Lysyl oxidase activity was also decreased in MD-MSCs during osteoblast differentiation. CONCLUSIONS: Our findings indicate that ATP7A dysfunction contributes to retardation in MSC development and impairs osteogenesis in MD.


Assuntos
Células-Tronco Pluripotentes Induzidas/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Osteogênese , Adenosina Trifosfatases/genética , Animais , Antígenos CD/genética , Antígenos CD/metabolismo , Proteínas de Transporte de Cátions/genética , Células Cultivadas , ATPases Transportadoras de Cobre , Endoglina , Matriz Extracelular/metabolismo , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/transplante , Lactente , Recém-Nascido , Síndrome dos Cabelos Torcidos/genética , Síndrome dos Cabelos Torcidos/patologia , Camundongos , Mutação , Proteína-Lisina 6-Oxidase/metabolismo , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo
18.
Am J Physiol Cell Physiol ; 309(10): C660-8, 2015 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-26269458

RESUMO

Menkes disease is a fatal neurodegenerative disorder arising from a systemic copper deficiency caused by loss-of-function mutations in a ubiquitously expressed copper transporter, ATP7A. Although this disorder reveals an essential role for copper in the developing human nervous system, the role of ATP7A in the pathogenesis of signs and symptoms in affected patients, including severe mental retardation, ataxia, and excitotoxic seizures, remains unknown. To directly examine the role of ATP7A within the central nervous system, we generated Atp7a(Nes) mice, in which the Atp7a gene was specifically deleted within neural and glial cell precursors without impairing systemic copper homeostasis, and compared these mice with the mottled brindle (mo-br) mutant, a murine model of Menkes disease in which Atp7a is defective in all cells. Whereas mo-br mice displayed neurodegeneration, demyelination, and 100% mortality prior to weaning, the Atp7a(Nes) mice showed none of these phenotypes, exhibiting only mild sensorimotor deficits, increased anxiety, and susceptibility to NMDA-induced seizure. Our results indicate that the pathophysiology of severe neurological signs and symptoms in Menkes disease is the result of copper deficiency within the central nervous system secondary to impaired systemic copper homeostasis and does not arise from an intrinsic lack of ATP7A within the developing brain. Furthermore, the sensorimotor deficits, hypophagia, anxiety, and sensitivity to NMDA-induced seizure in the Atp7a(Nes) mice reveal unique autonomous requirements for ATP7A in the nervous system. Taken together, these data reveal essential roles for copper acquisition in the central nervous system in early development and suggest novel therapeutic approaches in affected patients.


Assuntos
Adenosina Trifosfatases/metabolismo , Proteínas de Transporte de Cátions/metabolismo , Cobre/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Adenosina Trifosfatases/genética , Animais , Proteínas de Transporte de Cátions/genética , ATPases Transportadoras de Cobre , Feminino , Regulação da Expressão Gênica/fisiologia , Integrases , Masculino , Síndrome dos Cabelos Torcidos/genética , Síndrome dos Cabelos Torcidos/patologia , Camundongos , Camundongos Knockout , Mutação
19.
Chem Commun (Camb) ; 51(26): 5556-71, 2015 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-25647245

RESUMO

Copper ions are indispensible to life and maintaining tight control over the homeostasis of copper ions in the body is a prerequisite to sustaining health. Aberrations in normal copper levels, both systemic as well as on a tissue or cellular scale, are implicated in a wide range of diseases, such as Menkes disease, Wilson's disease, Alzheimer's disease, Parkinson's disease and transmissible spongiform encephalopathy (prion diseases). The current understanding of how copper influences these diseases is described. The field of fluorescent copper sensors both functioning via a reaction based mechanism as well as by directly binding copper ions has known an inflation in recent years, and the importance of this field to elucidating the role of copper in cell biology is pointed out. Progress in these tightly interwoven fields has resulted in a better understanding of a number of diseases related to copper imbalances and current developments might open the path for novel and innovating therapies to address these diseases.


Assuntos
Cobre/análise , Cobre/metabolismo , Corantes Fluorescentes , Doença de Alzheimer/metabolismo , Doença de Alzheimer/fisiopatologia , Cobre/química , Corantes Fluorescentes/análise , Corantes Fluorescentes/química , Degeneração Hepatolenticular/metabolismo , Degeneração Hepatolenticular/fisiopatologia , Humanos , Íons/análise , Íons/química , Íons/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Síndrome dos Cabelos Torcidos/fisiopatologia , Simulação de Dinâmica Molecular , Estrutura Molecular , Doença de Parkinson/metabolismo , Doença de Parkinson/fisiopatologia , Doenças Priônicas/metabolismo , Doenças Priônicas/fisiopatologia
20.
J Trace Elem Med Biol ; 31: 173-7, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25172213

RESUMO

Mutations in ATP7A lead to at least three allelic disorders: Menkes disease (MD), Occipital horn syndrome and X-linked distal motor neuropathy. These disorders are mainly seen in male individuals, but a few affected females have been described. More than 400 different mutations have been identified in the ATP7A gene. We have conducted several studies in the hope of uncovering the relationship between genotype and phenotype. We have examined the X-inactivation pattern in affected females, the effect of exon-deletions and--duplications, and splice-site mutations on the composition and amount of ATP7A transcript, and we have examined the structural location of missense mutations. The X-inactivation pattern did not fully explain the manifestation of MD in a small fraction of carriers. Most of the affected females had preferential inactivation of the X-chromosome with the normal ATP7A gene, but a few individuals exhibited preferential inactivation of the X-chromosome with the mutated ATP7A gene. The observed mild phenotype in some patients with mutations that effect the composition of the ATP7A transcript, seems to be explained by the presence of a small amount of normal ATP7A transcript. The location of missense mutations on structural models of the ATP7A protein suggests that affected conserved residues generally lead to a severe phenotype. The ATP7A protein traffics within the cells. At low copper levels, ATP7A locates to the Trans-Golgi Network (TGN) to load cuproenzymes with copper, whereas at higher concentrations, ATP7A shifts to the post-Golgi compartments or to the plasma membrane to export copper out of the cell. Impaired copper-regulation trafficking has been observed for ATP7A mutants, but its impact on the clinical outcome is not clear. The major problem in patients with MD seems to be insufficient amounts of copper in the brain. In fact, prenatal treatment of mottled mice as a model for human MD with a combination of chelator and copper, produces a slight increase in copper levels in the brain which perhaps leads to longer survival and more active behavior. In conclusion, small amounts of copper at the right location seem to relieve the symptoms.


Assuntos
Adenosina Trifosfatases/metabolismo , Proteínas de Transporte de Cátions/metabolismo , Cobre/metabolismo , Cútis Laxa/metabolismo , Síndrome de Ehlers-Danlos/metabolismo , Doenças Genéticas Ligadas ao Cromossomo X/metabolismo , Neuropatia Hereditária Motora e Sensorial/metabolismo , Síndrome dos Cabelos Torcidos/metabolismo , Mutação , Adenosina Trifosfatases/genética , Animais , Encéfalo/metabolismo , Proteínas de Transporte de Cátions/genética , Quelantes/uso terapêutico , Cobre/deficiência , Cobre/uso terapêutico , ATPases Transportadoras de Cobre , Cútis Laxa/genética , Cútis Laxa/fisiopatologia , Cútis Laxa/terapia , Deficiências Nutricionais/dietoterapia , Deficiências Nutricionais/etiologia , Suplementos Nutricionais , Regulação para Baixo , Síndrome de Ehlers-Danlos/genética , Síndrome de Ehlers-Danlos/fisiopatologia , Síndrome de Ehlers-Danlos/terapia , Feminino , Estudos de Associação Genética , Doenças Genéticas Ligadas ao Cromossomo X/genética , Doenças Genéticas Ligadas ao Cromossomo X/fisiopatologia , Doenças Genéticas Ligadas ao Cromossomo X/terapia , Neuropatia Hereditária Motora e Sensorial/genética , Neuropatia Hereditária Motora e Sensorial/fisiopatologia , Neuropatia Hereditária Motora e Sensorial/terapia , Humanos , Masculino , Síndrome dos Cabelos Torcidos/genética , Síndrome dos Cabelos Torcidos/fisiopatologia , Síndrome dos Cabelos Torcidos/terapia , Neurônios/metabolismo , Índice de Gravidade de Doença , Inativação do Cromossomo X
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