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1.
PLoS One ; 8(6): e64938, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23762268

RESUMO

The Asian citrus psyllid (ACP), Diaphorina citri (Hemiptera: Psyllidae) is the primary vector of the bacterium causing citrus huanglongbing (citrus greening), the most serious disease of citrus worldwide. Psyllids and other hemipterans produce large amounts of honeydew, which has been used previously as an indicator of phloem sap composition and insect feeding or metabolism. Behavioral, ultrastructural and chemical studies on ACP, its honeydew and waxy secretions showed important differences between nymphs, males and females, and suggested some mechanisms by which the psyllids, especially nymphs and adult females, can minimize their contamination with honeydew excretions. The anal opening in ACP, near the posterior end of the abdomen, is on the ventral side in nymphs and on the dorsal side in adult males and females. Video recordings showed that adult males produce clear sticky droplets of honeydew gently deposited behind their body on the leaf surface, whereas adult females produce whitish honeydew pellets powerfully propelled away from the female body, probably to get their excretions away from eggs and newly hatched nymphs. ACP nymphs produce long ribbons or tubes of honeydew that frequently stay attached to the exuviae after molting, or drop when feeding on the lower side of citrus leaves. Furthermore, honeydew excretions of both nymphs and adult females are covered with a thin layer of whitish waxy material ultrastructurally composed of a convoluted network of long fine filaments or ribbons. This material is extruded from intricate arrays of wax pores in the circumanal ring (around the anus) that is found in nymphs and females but not in males of ACP or other psyllid species. Infrared microscopy and mass spectroscopy revealed that, in addition to various sugars, honeydew excretions of ACP nymphs and females are covered with a thin layer of wax similar in profile to ester waxes.


Assuntos
Sacos Anais/fisiologia , Comportamento Animal , Secreções Corporais/química , Citrus/microbiologia , Hemípteros/fisiologia , Sacos Anais/ultraestrutura , Animais , Feminino , Cadeia Alimentar , Interações Hospedeiro-Patógeno , Masculino , Microscopia Eletrônica de Varredura , Ninfa , Espectrofotometria Infravermelho
2.
Acta Histochem ; 114(4): 327-33, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21802714

RESUMO

The distribution of sialic acids and antimicrobial products (lysozyme, ß-defensin-1, lactoferrin, IgA) in the anal glands of miniature pig was studied by glycoconjugate histochemistry and immunohistochemistry. The glandular acini of these glands exhibited considerable amounts of sialoglycoconjugates that terminated in Siaα2-6Gal/GalNAc or Siaα2-3Gal1-4GlcNAc, including O-acetylated sialic acids. Additionally, all antimicrobial products examined could be demonstrated in the anal glands, especially in the serous cells. The results obtained are discussed with regard to the functional significance of the anal glands. Our observations corroborated the view that sialic acids closely interact with defense cells and antimicrobial substances in the innate immune response. Therefore, the anal glandular secretions may function as protective agents in order to preserve the integrity of the anal region.


Assuntos
Sacos Anais/ultraestrutura , Imunidade Inata , Ácidos Siálicos/análise , Sacos Anais/imunologia , Animais , Glicoconjugados/análise , Imunoglobulina A/análise , Imunoglobulina A/biossíntese , Imuno-Histoquímica , Lactoferrina/análise , Lactoferrina/biossíntese , Masculino , Muramidase/análise , Muramidase/biossíntese , Coloração e Rotulagem , Suínos , Porco Miniatura , beta-Defensinas/análise , beta-Defensinas/biossíntese
3.
Cells Tissues Organs ; 168(3): 203-19, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11173805

RESUMO

The study reports on secretion production and composition in the tubular glands of the canine anal sacs. For this purpose, light and electron microscopical (TEM, SEM) as well as several histochemical methods for the demonstration of lysosomal acidity, lipofuscin, and complex carbohydrates were used. The glandular tubules exhibited a pseudostratified epithelium with secretory cells of a different shape as related to secretion production activity, and regionally varying amounts of basal cells. Flat, cuboidal or columnar cells with or without apocrine-like protrusions were assembled in one glandular endpiece, although grouping of these cell types often occurred. Active secretory cells were columnar with many cytoplasmic vesicles and a typically merocrine and/or micro-apocrine exocytosis of vesicle contents. Additionally, many lysosomes of different sizes could be found, whereby in aged cells giant secondary lysosomes (autophagolysosomes, about 7 microm in diameter) occupied the major cell part. These giant lysosomes were shed by an apocrine-like process forming a final bottleneck stage of the upper cell part, and consisted of ceroid-type lipofuscin. The general carbohydrate histochemical and the lectin histochemical methods revealed that the secretion produced was composed of strongly concentrated neutral glycoproteins with the following saccharide residues: alpha-D-mannose, beta-D-galactose, beta-N-acetyl-D-glucosamine, alpha-L-fucose and N-acetyl-neuraminic acid (sialic acid); the luminal secretion contained only beta-D-galactose and, especially, N-acetyl-neuraminic acid. This luminal secretion showed a spatially orientated maturation beginning in terminal tubular regions and finishing near the excretory duct, independent of the different secretory cell types. The results obtained demonstrated highly active secretion production, with a regional variation in the glandular tubule, and at least three different modes of secretion by the secretory cells, whereby the shedding of giant lipofuscin granules seems to be very specific. The high amounts of sialic acids in the glycoproteins found may influence the rheological properties of the secretion by their water-binding capacities.


Assuntos
Sacos Anais/citologia , Sacos Anais/metabolismo , Glândulas Exócrinas/citologia , Glândulas Exócrinas/metabolismo , Lectinas/química , Lectinas/metabolismo , Sacos Anais/química , Sacos Anais/ultraestrutura , Animais , Líquidos Corporais/química , Líquidos Corporais/metabolismo , Cães , Glândulas Exócrinas/química , Glândulas Exócrinas/ultraestrutura , Feminino , Histocitoquímica , Lectinas/ultraestrutura , Masculino , Microscopia Eletrônica , Microscopia Eletrônica de Varredura
4.
Mem Inst Oswaldo Cruz ; 79(4): 513-5, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6442753

RESUMO

Epimastigotes multiplying extracellularly and metacyclic trypomastigotes, stages that correspond to the cycle of Trypanosoma cruzi in the intestinal lumen of its insect vector, were consistently found in the lumen of the anal glands of opossums Didelphis marsupialis inoculated subcutaneously with infective feces of triatomid bugs.


Assuntos
Sacos Anais/parasitologia , Gambás/parasitologia , Trypanosoma cruzi/fisiologia , Sacos Anais/ultraestrutura , Animais , Doença de Chagas/veterinária , Reservatórios de Doenças , Feminino , Interações Hospedeiro-Parasita , Técnicas In Vitro
5.
Am J Pathol ; 107(2): 167-75, 1982 May.
Artigo em Inglês | MEDLINE | ID: mdl-7200729

RESUMO

Adenocarcinomas derived from apocrine glands of the anal sac and associated with persistent hypercalcemia in dogs were composed of tumor cells with numerous profiles of rough endoplasmic reticulum, clusters of free ribosomes, and a prominent Golgi apparatus. Neoplastic cells contained microtubules, microfilaments, tonofibrils, and had two types of electron-dense granules. Large lysosomelike dense bodies ranged from 0.6 to 2.2 microns in diameter and had a poorly delineated limiting membrane. Small granules (150-400 nm in diameter) had a sharply delineated limiting membrane with a narrow submembranous space and a homogeneous dense core. These smaller granules usually were located near the apexes of neoplastic cells, whereas the larger granules were situated near the base of cells. Apocrine cells in glands of the anal sac from control dogs that were in the secretory phase were columnar and had large dilated profiles of rough endoplasmic reticulum. Membranes of the endoplasmic reticulum fused with the plasmalemma and appeared to secrete their product directly into the lumens of acini, characteristic of merocrine secretion. Apical blebs of electron-lucent cytoplasm pinched off from nonneoplastic aprocine cells and were released into glandular lumens. Similar electron-lucent cytoplasmic blebs were present at the apexes of tumor cells. Myoepithelial cells were present between the epithelial cells and basement membrane in normal apocrine glands and were absent in neoplasms derived from these glands. Identification of the contents of the secretory-like granules in tumor cells and characterization of the hypercalcemic factor in the plasma or tumor tissue from dogs with this syndrome will help explain the pathogenesis of hypercalcemia associated with malignancy in animals and man.


Assuntos
Adenocarcinoma/veterinária , Neoplasias das Glândulas Anais/veterinária , Doenças do Cão/patologia , Hipercalcemia/veterinária , Neoplasias das Glândulas Sudoríparas/veterinária , Adenocarcinoma/complicações , Adenocarcinoma/ultraestrutura , Neoplasias das Glândulas Anais/complicações , Neoplasias das Glândulas Anais/ultraestrutura , Sacos Anais/ultraestrutura , Animais , Grânulos Citoplasmáticos/ultraestrutura , Citoesqueleto/ultraestrutura , Cães , Retículo Endoplasmático/ultraestrutura , Feminino , Complexo de Golgi/ultraestrutura , Hipercalcemia/etiologia , Ribossomos/ultraestrutura , Neoplasias das Glândulas Sudoríparas/complicações , Neoplasias das Glândulas Sudoríparas/ultraestrutura
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