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1.
Viruses ; 9(8)2017 07 25.
Artigo em Inglês | MEDLINE | ID: mdl-28757575

RESUMO

The Chinese giant salamander iridovirus (CGSIV), belonging to the genus Ranavirus in the family Iridoviridae, is the causative agent of an emerging infectious disease causing high mortality of more than 90% and economic losses in Chinese giant salamanders in China. In this study, a recombinant baculovirus-based vaccine expressing the CGSIV major capsid protein (MCP) was developed and its protective immunity in Chinese giant salamanders was evaluated. The recombinant Autographacalifornica nucleopolyhedrosis virus (AcNPV), expressing CGSIV MCP, designated as AcNPV-MCP, was generated with the highest titers of 1 × 108 plaque forming units/mL (PFU/mL) and confirmed by Western blot and indirect immunofluorescence (IIF) assays. Western blot analysis revealed that the expressed MCP reacted with mouse anti-MCP monoclonal antibodies at the band of about 53 kDa. The results of IIF indicated that the MCP was expressed in the infected Spodoptera frugiperda 9 (Sf9) cells with the recombinant baculovirus, and the Chinese giant salamander muscle cells also transduced with the AcNPV-MCP. Immunization with the recombinant baculovirus of AcNPV-MCP elicited robust specific humoral immune responses detected by ELISA and neutralization assays and potent cellular immune responses in Chinese giant salamanders. Importantly, the effective immunization conferred highly protective immunity for Chinese giant salamanders against CGSIV challenge and produced a relative percent of survival rate of 84%. Thus, the recombinant baculovirus expressing CGSIV MCP can induce significant immune responses involving both humoral and cell-mediated immunity in Chinese giant salamanders and might represent a potential baculovirus based vaccine candidate for Chinese giant salamanders against CGSIV.


Assuntos
Proteínas do Capsídeo/imunologia , Infecções por Vírus de DNA/veterinária , Ranavirus/imunologia , Salamandra/imunologia , Vacinas Virais/imunologia , Animais , Baculoviridae/imunologia , Proteínas do Capsídeo/genética , China , Infecções por Vírus de DNA/imunologia , Infecções por Vírus de DNA/prevenção & controle , DNA Viral , Imunidade Celular , Proteômica , Ranavirus/genética , Proteínas Recombinantes/administração & dosagem , Proteínas Recombinantes/imunologia , Salamandra/virologia , Vacinação/veterinária , Vacinas Sintéticas/administração & dosagem , Vacinas Sintéticas/imunologia , Vacinas Virais/administração & dosagem
2.
Ecohealth ; 8(3): 301-19, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22071720

RESUMO

Research that identifies the potential host range of generalist pathogens as well as variation in host susceptibility is critical for understanding and predicting the dynamics of infectious diseases within ecological communities. Ranaviruses have been linked to amphibian die-off events worldwide with the greatest number of reported mortality events occurring in the United States. While reports of ranavirus-associated mortality events continue to accumulate, few data exist comparing the relative susceptibility of different species. Using a series of laboratory exposure experiments and comparative phylogenetics, we compared the susceptibilities of 19 amphibian species from two salamander families and five anurans families for two ranavirus isolates: frog virus 3 (FV3) and an FV3-like isolate from an American bullfrog culture facility. We discovered that ranaviruses were capable of infecting 17 of the 19 larval amphibian species tested with mortality ranging from 0 to 100%. Phylogenetic comparative methods demonstrated that species within the anuran family Ranidae were generally more susceptible to ranavirus infection compared to species from the other five families. We also found that susceptibility to infection was associated with species that breed in semi-permanent ponds, develop rapidly as larvae, and have limited range sizes. Collectively, these results suggest that phylogeny, life history characteristics, and habitat associations of amphibians have the potential to impact susceptibility to ranaviruses.


Assuntos
Anuros/genética , Anuros/virologia , Infecções por Vírus de DNA/mortalidade , Suscetibilidade a Doenças , Ecossistema , Filogenia , Ranavirus/patogenicidade , Salamandra/genética , Salamandra/virologia , Animais , Doenças Transmissíveis Emergentes , Reservatórios de Doenças , Estados Unidos/epidemiologia
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