[Antirecurrence action of preparations made from Listeria in the surgical removal of sarcoma 180]. / Protivoretsidivnoe deistvie preparatov iz listerii pri khirurgicheskom udalenii sarkomy 180.

It is shown that in spite of the absence of the marked inhibition of the tumour growth in the preparative period listeria preparations decreased the percentage of recurrances. Based on the results obtained a conclusion is drawn that injection of listeria preparations into the tumour for several days before its surgical removal may be an effective prophylactic means of the relapse development.

Augmentation of host's immunity by combined cryodestruction of sarcoma 180 and administration of protein-bound polysaccharide, EA6, isolated from Flammulina velutipes (Curt. ex Fr.) Sing. in ICR mice.

Augmentation of the host's immunity by combined in situ freeze-destruction of the tumor (cryosurgery) and administration of the antitumor active protein-bound polysaccharide, EA6, isolated from a hot water extract of an edible mushroom, Flammulina velutipes (Curt. ex Fr.) Sing., was investigated in sarcoma 180 bearing ICR mice. Oral administration (p.o.) of the EA6 stimulated anti-sheep red blood cells (SRBC) IgM antibody-producing activity of the spleen cells, and also delayed hypersensitivity (DTH) against SRBC in swelling of the foot pads of the tumor-bearing hosts. When EA6 p.o. was combined with cryosurgery, further augmentation of IgM-producing activity and DTH reaction to SRBC was recognized as compared with the EA6 single use. But the reticuloendothelial system of the mice, estimated by carbon clearance test, was not activated by EA6 p.o. or combined with cryosurgery.

Augmentation of antitumor activity by combined cryo-destruction of sarcoma 180 and protein-bound polysaccharide, EA6, isolated from Flammulina velutipes (Curt. ex Fr.) Sing. in ICR mice.

Augmentation of antitumor activity by combined in situ freeze-destruction of tumor (cryosurgery) and administration of antitumor active substances isolated from a hot water extract of an edible mushroom, Flammulina velutipes (Curt. ex Fr.) SING., was investigated in sarcoma 180 bearing ICR mice. Antitumor active substances of the mushroom included beta-(1,3)-glucan (EA3) and protein-bound polysaccharide (EA6). Antitumor activity was evaluated by the growth rate of the solid tumor rechallenged subcutaneously (s.c.) or by cumulative mortalities of the mice rechallenged intraperitoneally (i.p.) with the ascites tumor, on day 7 after cryosurgery. Weak antitumor effect induced by cryosurgery against challenged solid tumor of sarcoma 180 was markedly augmented by i.p. administration of EA6 (10 mg/kg). Cryosurgery of the solid sarcoma 180 apparently did not induce any antitumor effect against challenged ascites sarcoma 180. However, when cryosurgery was combined with oral administration of the polysaccharides (50 mg/kg), prolonged survival of the mice challenged with ascites sarcoma 180 i.p. was recognized by EA6, but not by EA3. Timing of the administration of EA6 (i.p.) with cryosurgery was best in pre- and post-cryosurgery. Immunological activity of EA6 to the host was discussed.