RESUMO
AIM: to study the activity of antioxidant enzymes and to evaluate an intensity of prooxidant processes in sarcoma 37 (S37) cells during tumor development and under influence of sodium dichloroacetate (SDA). METHODS: Activity of total superoxide dismutase (SOD), SOD isoforms, catalase (Cat), glutathione peroxidase (GP), and glutathione reductase (GR), as well as content of reduced glutathione (GSH) and lipid peroxidation (LP) secondary byproducts were determined in S37 homogenated tissues of untreated mice and animals treated with SDA at daily dose of 86 mg/kg. RESULTS: SDA treatment of S37-bearing mice resulted in the reduced activities of total SOD, SOD isoforms (especially Mn-SOD), Cat, GP and significantly decreased GSH content on the background of LP intensification in tumor tissue. CONCLUSION: The observed changes of oxidative homeostasis in S37-bearing animals treated with SDA could be considered as an element of antitumor action of SDA.
Assuntos
Ácido Dicloroacético/farmacologia , Sarcoma 37/metabolismo , Animais , Catalase/metabolismo , Proliferação de Células/efeitos dos fármacos , Ácido Dicloroacético/administração & dosagem , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Glutationa Redutase/metabolismo , Injeções Intraperitoneais , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Oxirredução , Sarcoma 37/tratamento farmacológico , Sarcoma 37/mortalidade , Superóxido Dismutase/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismoRESUMO
Oral administration of a liposome-encapsulated agent triggering the synthesis of interferon--poly(I) poly (C)--to mice bearing solid sarcoma 37 was followed by (a) production of an interferon which circulates for a longer period after preliminary administration of "empty" liposomes; (b) lengthening of the period of tumor latency; in some cases tumor failed to appear at all; (c) higher efficacy of radiation treatment which was manifested by a higher inhibition of tumor growth or complete regression; (d) increased antitumor effect of 5-fluorouracil, and (e) suppression of proliferative activity of tumor.
Assuntos
Fluoruracila/administração & dosagem , Indutores de Interferon/administração & dosagem , Sarcoma 37/terapia , Sarcoma Experimental/terapia , Animais , Ciclo Celular/efeitos dos fármacos , Ciclo Celular/efeitos da radiação , Terapia Combinada , Lipossomos/administração & dosagem , Camundongos , Transplante de Neoplasias , Poli I-C/administração & dosagem , Dosagem Radioterapêutica , Sarcoma 37/mortalidade , Fatores de TempoRESUMO
Athymic nude (nu/nu) mice, normal NMRI (plus/plus) mice, and nu/plus heterozygotes, inoculated at birth with the Moloney strain of murine sarcoma virus (M-MSV), did not differ either in tumor growth rate or incidence or in mortality due to tumor development; neither did nude (nu/nu) and normal NMRI mice inoculated with M-MSV at 30 days of age differ in the initial tumor growth rate. However, tumors induced in adult nude mice grew progressively and eventually killed the hosts, whereas tumors induced in adult normal NMRI mice completely regressed. These results indicated that thymus-dependent immunity, though not active in M-MSV tumor induction, determined whether the tumor, once formed, would regress or kill the host.
