RESUMO
OBJECTIVE: To obtain polyploidy Scutellaria barbata. METHODS: Explants of stem segment of Scutellaria barbata were treated with oryzalin at various concentration and time duration in vitro. RESULTS: Comparing with the characteristics of diploid, mutant plants had significant changes in plant morphology inducing compact plant, darker leaf color, smaller leaf index, thicken stem, significantly larger stomata, guard cells and chloroplasts in stomata guard cells and significantly lower stomata density. Mutant plants were plant chimera of diploid and tetraploid assessed by chromosome counting and flow cytometry. CONCLUSION: The technique could be used for chromosome doubling in Scutellaria barbata.
Assuntos
Cromossomos de Plantas/efeitos dos fármacos , Dinitrobenzenos/farmacologia , Poliploidia , Scutellaria/fisiologia , Sulfanilamidas/farmacologia , Cromossomos de Plantas/genética , Cruzamentos Genéticos , Diploide , Folhas de Planta/efeitos dos fármacos , Folhas de Planta/fisiologia , Caules de Planta/efeitos dos fármacos , Caules de Planta/fisiologia , Scutellaria/efeitos dos fármacos , Scutellaria/genética , Fatores de Tempo , Técnicas de Cultura de Tecidos/métodosRESUMO
Tissue cultures of Scutellaria amoena were established from the young stem explants on MS + IAA 0.1 mg/L + BA 0. 5 (0.2) mg/L. Propagation cultures were established on MS + BA 0.2 (1.5) mg/L + IAA 0.1 mg/L + NAA 0.1 mg/L + 2, 4-D 0.5 (1.5) mg/L. Rooting of planlet was established on 1/2 MS + NAA 0.1 (0.2) mg/L + IBA 0.1 (0.2) mg/L + PP333 1.0 mg/L and the rate can reach 100%.
