Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Int J Biol Macromol ; 184: 522-529, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34119553

RESUMO

Sericin, as the main component of silkworm cocoon silk, surrounds and protects the silk fibroin. Sericin is a natural macromolecular protein complex encoded by the genes Ser1, Ser2, and Ser3. At present, there are no available antibodies against sericin that may be used to identify and locate it at the protein level, hindering the study of its secretion mechanism and materials application. Therefore, the development of effective antibodies against sericin is an urgent necessity. To address this problem, we prepared polyclonal antibodies against the Ser1, Ser2 and Ser3 proteins using synthesized peptides for the first time. The specificity of the antibodies was confirmed using dot blot, immunoblotting and mass spectrometry on the hybrid bands of the middle silk gland. The immunoblotting results of anti-sericin antibodies showed that sericin has different molecular weights in different regions of the middle silk gland and strains in the 5th instar. Through immunohistochemistry, anti-sericin antibodies revealed that sericin presented different distributions in the anterior part of the middle silk gland of 872 strain at the 7th day of 5th instar. In addition, the prepared antibodies not only detected intact sericin molecules, but also detected degraded sericin that was dissolved in five different solvents. In summary, this work prepared effective sericin antibodies for silk protein synthesis and secretion research and provides a possible molecular detection method for biological products containing silkworm sericin.


Assuntos
Anticorpos/análise , Bombyx/crescimento & desenvolvimento , Peptídeos/imunologia , Sericinas/química , Sequência de Aminoácidos , Animais , Especificidade de Anticorpos , Bombyx/imunologia , Bombyx/metabolismo , Peso Molecular , Família Multigênica , Peptídeos/genética , Sericinas/genética , Sericinas/imunologia , Especificidade da Espécie
2.
Macromol Biosci ; 17(12)2017 12.
Artigo em Inglês | MEDLINE | ID: mdl-29045024

RESUMO

The biosafety of sericin remains controversial. The misunderstanding regarding sericin causing adverse biological responses have been clarified by extensively reviewing relevant literatures and experimentally demonstrating that sericin exhibits mild inflammatory responses, negligible allergenicity, and low immunogenicity in vivo. This study supports that sericin is biosafe as a natural biomaterial.


Assuntos
Materiais Biocompatíveis/efeitos adversos , Sericinas/imunologia , Sericinas/farmacologia , Imunidade Adaptativa/efeitos dos fármacos , Animais , Bombyx/genética , Hipersensibilidade a Drogas/etiologia , Fibroínas/imunologia , Fibroínas/farmacologia , Imunidade Inata/efeitos dos fármacos , Inflamação/induzido quimicamente , Masculino , Teste de Materiais , Camundongos , Camundongos Endogâmicos BALB C , Mutação , Células RAW 264.7 , Sericinas/efeitos adversos
3.
Histol Histopathol ; 31(9): 1011-27, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-26864661

RESUMO

Silk sericin-releasing (sericin/polyvinyl alcohol (PVA)/glycerin) scaffolds have been designed for wound dressing applications using different fabrication techniques that influence scaffold antigenicity. The immunological tolerance of scaffolds depends on the balance of immunogenic and tolerogenic responses modulated by dendritic cells (DCs). An in vivo skin implantation model was used to compare the tolerogenic effect of sericin/PVA/glycerin scaffolds prepared by freeze-drying versus salt-leaching techniques, using an Allevyn® scaffold as a control. Immunohistochemical and histopathological studies were performed to evaluate tolerogenic DCs (CD206+), immunogenic DCs (CD83+), regulatory T-cells (FOXP3+ and CTLA-4), a proinflammatory cytokine (interleukin 33: IL-33), a stress marker (heat shock protein 60; HSP60), histopathological changes and related inflammatory cells. It was found that both sericin/PVA/glycerin scaffolds were tolerogenic and induced early activated Treg functions, while the Allevyn® scaffold was immunogenic. However, the tolerance of the freeze-dried sericin/PVA/glycerin scaffolds was not as consistent as the salt-leached sericin/PVA/glycerin scaffolds, indicated by the low level of CTLA-4 expression. This was probably due to molecular cross-linking and the morphological and mechanical properties of the freeze-drying technique, which would enhance the immune response. Severe inflammatory responses (including mast cell degranulation and foreign body giant cell accumulation) and histopathological changes (including fat infiltration and fibrosis formation) were mainly found with the Allevyn® scaffold, presumably from its architecture and chemical composition, especially polyurethane. The up-regulation of IL-33 and HSP60 with the Allevyn® scaffold was correlated with the inflammatory and pathological levels. Our findings suggested that salt-leached sericin/PVA/glycerin scaffolds were tolerogenic, induced a low inflammatory response and were appropriate for wound dressing applications.


Assuntos
Materiais Biocompatíveis/química , Células Dendríticas/imunologia , Tolerância Imunológica/imunologia , Teste de Materiais , Alicerces Teciduais/química , Animais , Antígenos CD/imunologia , Antígeno CTLA-4/imunologia , Chaperonina 60/imunologia , Feminino , Fatores de Transcrição Forkhead/imunologia , Glicerol/imunologia , Imunoglobulinas/imunologia , Imuno-Histoquímica , Interleucina-33/imunologia , Lectinas Tipo C/imunologia , Receptor de Manose , Lectinas de Ligação a Manose/imunologia , Glicoproteínas de Membrana/imunologia , Proteínas Mitocondriais/imunologia , Álcool de Polivinil , Ratos , Ratos Wistar , Receptores de Superfície Celular/imunologia , Sericinas/imunologia , Cicatrização , Antígeno CD83
4.
J Control Release ; 115(3): 307-15, 2006 Oct 27.
Artigo em Inglês | MEDLINE | ID: mdl-17034892

RESUMO

When silk fiber derived from Bombyx mori was subjected to degumming treatments twice in water and subsequent degraded processing in slightly alkaline aqueous solution under high-temperature and high-pressure, the water-soluble silk sericin peptides (SS) with different molecular mass from 10 to 70 kDa were obtained. The sericin peptides could be conjugated covalently with insulin alone with cross-linking reagent glutaraldehyde. The physicochemical properties of the silk sericin-insulin (SS-Ins) conjugates were determined by Enzyme-Linked Immunosorbent Assay (ELISA). The biological activities of SS-Ins bioconjugates were investigated in vitro and in vivo. The results in human serum in vitro indicated that the half-life of the synthesized SS-Ins derivatives was 2.3 and 2.7 times more than that of bovine serum albumin-insulin (BSA-Ins) conjugates and intact insulin, respectively. The pharmacological activity of SS-Ins bioconjugates lengthened to 21 h in mice in vivo, which was over 4 times longer than that of the native insulin. The immunogenicity of silk sericin and the antigenicity of SS-Ins derivatives were not observed in both rabbits and mice. The bioconjugation of insulin with silk sericin protein evidently improved both physicochemical and biological stability of the polypeptide.


Assuntos
Bombyx , Hipoglicemiantes/síntese química , Proteínas de Insetos/química , Insulina/síntese química , Sericinas/química , Seda/química , Sequência de Aminoácidos , Animais , Glicemia/efeitos dos fármacos , Glicemia/metabolismo , Química Farmacêutica , Reagentes de Ligações Cruzadas/química , Diabetes Mellitus Experimental/sangue , Estabilidade de Medicamentos , Glutaral/química , Hipoglicemiantes/imunologia , Hipoglicemiantes/farmacologia , Técnicas In Vitro , Proteínas de Insetos/imunologia , Proteínas de Insetos/isolamento & purificação , Insulina/análogos & derivados , Insulina/imunologia , Insulina/farmacologia , Masculino , Camundongos , Peso Molecular , Coelhos , Sericinas/imunologia , Sericinas/isolamento & purificação , Soroalbumina Bovina/química
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...