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1.
J Chromatogr ; 416(2): 219-35, 1987 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-3611255

RESUMO

The gas chromatographic and mass spectrometric properties of the monohydroxy acids 5-hydroxyeicosatetraenoic acid (5-HETE), 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-hydroxyeicosatetraenoic acid (15-HETE) as their methyl ester trimethylsilyl, methyl ester allyldimethylsilyl and methyl ester tert.-butyldimethylsilyl ethers were investigated. The gas chromatographic properties of the trimethylsilyl and tert.-butyldimethylsilyl derivatives were found to be excellent while the allyldimethylsilyl derivative required a well deactivated column. The mass spectra of these silyl derivatives with the exception for 12-HETE did not exhibit particularly intense ions in the upper mass region. A quantitative analysis by selected-ion monitoring of the most intense ion in the upper mass region of respective mass spectrum demonstrated that a detection limit in the low picogram range could only be obtained for 12-HETE. Since the mass spectra indicated that the double bonds exerted a strong influence on the fragmentation pattern, the trimethylsilyl, allyldimethylsilyl and tert.-butyldimethylsilyl ethers of the methyl esters of the reduced analogues of the monohydroxy acids were prepared. The saturation of the double bonds completely altered the fragmentation patterns and very intense ions carrying a high percentage of the total ion abundance were found in all of the mass spectra. The developed technique was utilized for measurements of 5-HETE in lung tissue samples from patients with lung cancer.


Assuntos
Ácidos Hidroxieicosatetraenoicos/análise , Compostos de Organossilício , Ácido 12-Hidroxi-5,8,10,14-Eicosatetraenoico , Compostos Alílicos/análise , Compostos Alílicos/síntese química , Catálise , Cromatografia Gasosa , Ésteres/análise , Ésteres/síntese química , Cromatografia Gasosa-Espectrometria de Massas , Humanos , Hidrogenação , Pulmão/análise , Silício/análise , Silício/síntese química , Compostos de Trimetilsilil/análise , Compostos de Trimetilsilil/síntese química
4.
Int J Appl Radiat Isot ; 36(5): 375-8, 1985 May.
Artigo em Inglês | MEDLINE | ID: mdl-4018898

RESUMO

18F-Labeled fluorotrimethylsilane was prepared by nucleophilic substitution of chlorotrimethylsilane with reactor produced [18F]fluoride. Hydrolysis of fluorotrimethylsilane by aqueous tetraethylammonium hydroxide followed by removal of water with a mechanical pump gave a powerful source of no carrier added nucleophilic 18F. Reaction of this purified 18F preparation with 4,6-benzylidene-1-beta-O-methyl D-mannopyranoside-2.3-cyclic sulfate was complete in 2 min at 80 degrees C and gave two labeled products with similar retention times on reverse phase HPLC. Allowing for decay and handling losses during deprotection, the maximum yield of [18F]2-deoxy-2-fluoro-D-glucose from no-carrier added tetraethylammonium fluoride was 50%. Incorporation of 18F into organic products was 30% complete in 10 min at room temperature. An identical time-course was observed for reaction of 3-O-triflyl-1,2-5,6-diisopropylidene-D-allofuranose, the starting material for 3-deoxy-3-fluoro-D-glucose. Reaction of tetraethylammonium fluoride with chlorotrimethylsilane was more rapid and much more tolerant of water than the fluorosugar reactions. Chlorotrimethylsilane can be used to recover unreacted 18F from reaction mixtures.


Assuntos
Desoxiaçúcares/síntese química , Desoxiglucose/síntese química , Silício/síntese química , Compostos de Tetraetilamônio , Compostos de Trimetilsilil/síntese química , Fenômenos Químicos , Química , Cromatografia Líquida de Alta Pressão , Desoxiglucose/análogos & derivados , Flúor , Fluordesoxiglucose F18 , Hidrólise , Marcação por Isótopo , Cinética , Radioisótopos , Tetraetilamônio
5.
Scand J Work Environ Health ; 11(2): 111-5, 1985 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-4001899

RESUMO

Airborne dust from the production of silicon carbide has been analyzed for particle morphology and composition. Fibers of alpha silicon carbide were identified by scanning electron microscopy (SEM) combined with energy dispersive X-ray spectrometry (EDS) and transmission electron microscopy (TEM) with selected area electron diffraction techniques (SAED). Micrographs taken at high magnification revealed several stacking periods along the fiber axis, and one or more of the polytypes 2H, 4H, or 6H could be distinguished. Preliminary investigations applying SEM showed that 80% of the fibers had diameters of less than 0.5 micron and a length greater than 5 micron. Fiber concentrations were examined by the counting of stationary and personal samples in an optical phase contrast microscope. The fiber levels in the three plants investigated were low and less than 1 fiber/cc of air (10(6) fibers/m3). Dust samples from the handling of raw material, including recycled material, contained up to 5 fibers/cc (5 X 10(6) fibers/m3).


Assuntos
Poluentes Ocupacionais do Ar/análise , Compostos Inorgânicos de Carbono , Carbono/análise , Compostos de Silício , Silício/análise , Carbono/síntese química , Microscopia Eletrônica de Varredura , Silício/síntese química
6.
Steroids ; 45(3-4): 357-74, 1985.
Artigo em Inglês | MEDLINE | ID: mdl-3834657

RESUMO

Androst-4-en-3-one analogs incorporating a trimethylsilyl or a trimethylsilylmethyl group at C-1, C-2 or C-19 were prepared and evaluated as inhibitors of aromatase. Only 10-[1-hydroxy-2-(trimethylsilyl)ethyl]estr-4-ene-3,17-dione inhibited human placental aromatase. Enzyme kinetic analysis revealed competitive inhibition [apparent dissociation constant (Ki) of 562 +/- 12 nM] associated with marginal time-dependent inhibition.


Assuntos
Androstenóis/síntese química , Inibidores da Aromatase , Placenta/enzimologia , Silício/síntese química , Compostos de Trimetilsilil/síntese química , Androstenóis/farmacologia , Ligação Competitiva , Fenômenos Químicos , Química , Feminino , Humanos , Indicadores e Reagentes , Cinética , Espectroscopia de Ressonância Magnética , Gravidez , Relação Estrutura-Atividade , Compostos de Trimetilsilil/farmacologia
7.
Bioorg Khim ; 10(12): 1645-54, 1984 Dec.
Artigo em Russo | MEDLINE | ID: mdl-6543305

RESUMO

Glycosylation of 5-trimethylsilyl- and 5-triethylsilyluracil as well as 5-trimethylsilylcytosine with 2-deoxy-3,5-di-O-p-toluyl-alpha-D-ribofuranosylchloride and subsequent deacylation led to alpha- and beta-anomers of 5-Me3SidUrd, 5-Et3SidUrd and 5-Me3SidCyd. 1-(alpha-D-Arabinofuranosyl)-5-trimethylsilyluracil was synthesized starting from the derivatives of 2,3,5-tri-O-benzoyl-D-arabinofuranose and then converted to alpha-5-Me3SidUrd via 1-(2,2'-anhydro-alpha-D-ribofuranosyl)-5-trimethylsilyluracil and 1-(2-chloro-2-deoxy-alpha-D-arabinofuranosyl)-5-trimethylsilyluracil+ ++. Out of all synthesized compounds, only alpha-5-Me3SidUrd inhibits the replication of HSV-1 both in vitro and in vivo; it also exerts a pronounced therapeutic effect in guinea pigs with herpes genitalis induced by HSV-2. The resistance of alpha-5-Me3SidUrd to pyrimidine phosphorylases facilitates its antiviral activity in vivo.


Assuntos
Encefalite/tratamento farmacológico , Herpes Genital/tratamento farmacológico , Herpes Simples/tratamento farmacológico , Nucleosídeos de Pirimidina/síntese química , Silício/síntese química , Compostos de Trimetilsilil/síntese química , Animais , Fenômenos Químicos , Química , Encefalite/etiologia , Feminino , Cobaias , Masculino , Nucleosídeos de Pirimidina/uso terapêutico , Ratos , Compostos de Trimetilsilil/uso terapêutico
8.
J Chromatogr ; 289: 195-206, 1984 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-6736153

RESUMO

Conditions are described for conversion of testosterone into the 3-enol, 17-bis-tert.-butyldimethylsilyl ether derivative without formation of side products. The steroid is treated with tert.- butyldimethylsilylimidazole in heptane at 100 degrees C using sodium formate as catalyst. Derivatives are also formed at different rates of 3-keto-5 alpha, 3 alpha/beta-hydroxy-, 6 alpha/beta-hydroxy-, 7 beta-hydroxy-, 16 alpha-hydroxy-, 17 beta-hydroxy(sec.)- and 20 alpha/beta-hydroxysteroids, whereas hydroxyl groups in 1 beta, 7 alpha, 12 alpha/beta, 15 beta and 17 alpha(tert.) positions do not react to a significant extent. These positions are derivatized by subsequent addition of trimethylsilylimidazole , yielding mixed derivatives which are suitable for gas chromatography--mass spectrometry with selected ion monitoring. Conditions are given for conversion of some biologically important androgens, progestins and bile acids into a single form of derivative. The use of the method is illustrated by an analysis of steroids in a rat testis.


Assuntos
Ácidos e Sais Biliares/análise , Compostos de Organossilício , Silício/síntese química , Esteroides/análise , Compostos de Trimetilsilil/síntese química , Animais , Cromatografia Gasosa/métodos , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Masculino , Ratos , Solventes , Testículo/análise
10.
Br J Ind Med ; 41(1): 109-15, 1984 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-6691928

RESUMO

Chest x rays, smoking histories, and pulmonary function tests were obtained for 171 men employed in the manufacturing of silicon carbide. A lifetime exposure to respirable particulates (organic and inorganic fractions) and sulphur dioxide was estimated for each worker. Chest x ray abnormalities were related to respirable particulates (round opacities) and to age and smoking (linear opacities). Pulmonary function was affected by respirable particulates (FVC) and by sulphur dioxide and smoking (FEV1). Pleural thickening was related to age. No exposures exceeded the relevant standards; we therefore conclude that the current standards do not provide protection against injurious pulmonary effects, at least in this industry.


Assuntos
Compostos Inorgânicos de Carbono , Carbono/síntese química , Indústria Química , Doenças Profissionais/etiologia , Doenças Respiratórias/etiologia , Compostos de Silício , Silício/síntese química , Adulto , Poluentes Ocupacionais do Ar/efeitos adversos , Poluentes Ocupacionais do Ar/análise , Exposição Ambiental , Humanos , Pulmão/diagnóstico por imagem , Masculino , Pessoa de Meia-Idade , Radiografia , Testes de Função Respiratória , Fumar , Dióxido de Enxofre/efeitos adversos , Dióxido de Enxofre/análise
11.
Nucleic Acids Symp Ser ; (15): 41-4, 1984.
Artigo em Inglês | MEDLINE | ID: mdl-6522290

RESUMO

Formation of 3',5'-O-(dialkylsilanediyl)deoxyribonucleosides was studied. Treatment of deoxythymidine in DMF with bifunctional silylating reagent such as di-t-butyldichlorosilane and diisopropyldichlorosilane in the presence of imidazole gave the expected silanediyl derivatives. The structure was confirmed mainly by NMR spectroscopy. The stability of these cyclic silyl derivatives toward hydrolysis is also described.


Assuntos
Desoxirribonucleosídeos/síntese química , Silanos/síntese química , Silício/síntese química , Estabilidade de Medicamentos , Indicadores e Reagentes , Espectroscopia de Ressonância Magnética , Relação Estrutura-Atividade
13.
Hoppe Seylers Z Physiol Chem ; 362(9): 1289-92, 1981 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-7346386

RESUMO

2-(Trimethylsilyl)ethyl-4-nitrophenyl carbonate has been prepared as a new reagent for the introduction of the 2-(trimethylsilyl)ethyloxycarbonyl group into amino acids or amino acid derivatives. The resulting N alpha-protected amino acids were found to represent suitable intermediates for the synthesis of peptides. The amino-protecting group proved to be stable under the usual conditions of peptide synthesis and readily cleaved in selective conditions via fluoride ion-mediated fragmentation.


Assuntos
Aminoácidos/síntese química , Peptídeos/síntese química , Silício/síntese química , Compostos de Trimetilsilil/síntese química , Indicadores e Reagentes , Espectroscopia de Ressonância Magnética , Espectrofotometria Infravermelho
19.
Clin Chim Acta ; 73(3): 527-35, 1976 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-1000870

RESUMO

Evidence is presented to confirm that N,O-bis(trimethylsilyl)acetamide does not form trimethylsilyl derivatives of sodium salts of many organic acids. Further evidence suggests that such derivatives are formed when either hydroxylamine hydrochloride or trimethylchlorosilane are also present. This presents a useful technique of reducing losses of organic acids due to evaporation during lyophilisation by converting them to sodium salts and preparing volatile derivatives for gas chromatography directly from the salt residue.


Assuntos
Ácidos Carboxílicos/isolamento & purificação , Silício/síntese química , Sódio , Compostos de Trimetilsilil/síntese química , Cromatografia Gasosa/métodos , Hidroxilaminas
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