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1.
J Pharm Biomed Anal ; 177: 112859, 2020 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-31522098

RESUMO

Epinephrine and norepinephrine are a class of chiral endogenous catecholamines, which are known as major neurotransmitters. This work described a new LC-MS/MS method coupled with pre-column derivatization, enabling the simultaneous enantiomeric separation of epinephrine and norepinephrine in rat plasma. After protein precipitation procedure, the samples were derivatized with (S)-N-(4-nitrophenoxycarbonyl) phenylalanine methoxyethyl ester, [(S)-NIFE]. The derivatives resolved with good baseline separation on an ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 µm) with mobile phase composed of methanol with 0.2% formic acid in water at a flow rate of 0.2 mL/min. Analysis was performed by multiple reaction monitoring in positive ionization mode. The linear ranges were 1.0-500 ng/mL for epinephrine enantiomers and 1.5-750 ng/mL for norepinephrine enantiomers. The lower limits of quantification for epinephrine and norepinephrine enantiomers were 1.0 and 1.5 ng/mL, respectively. The intra-day and inter-day precision were all less than 10.7% and accuracy ranged from 96.0 to 101.5%. Recoveries for all the analytes were more than 80.3%. The proposed method was successfully applied to simultaneously determine endogenous epinephrine and norepinephrine enantiomers in rat plasma. l-epinephrine and l-norepinephrine were sensitively and accurately quantified while both the d-enantiomers were not detected. Additionally, epinephrine enantiomers were analyzed for stereoselective pharmacokinetics in rats after intravenous administration of racemic epinephrine for the first time. The pharmacokinetic results indicated that the disposition of epinephrine enantiomers was stereoselective and chiral inversion did not occur in rats.


Assuntos
Epinefrina/farmacocinética , Norepinefrina/farmacocinética , Simpatomiméticos/farmacocinética , Animais , Cromatografia Líquida de Alta Pressão/métodos , Epinefrina/administração & dosagem , Epinefrina/sangue , Epinefrina/química , Masculino , Modelos Animais , Estrutura Molecular , Norepinefrina/administração & dosagem , Norepinefrina/sangue , Norepinefrina/química , Ratos , Ratos Wistar , Organismos Livres de Patógenos Específicos , Estereoisomerismo , Relação Estrutura-Atividade , Simpatomiméticos/administração & dosagem , Simpatomiméticos/sangue , Simpatomiméticos/química , Espectrometria de Massas em Tandem/métodos
2.
Artigo em Inglês | MEDLINE | ID: mdl-28431781

RESUMO

Clenbuterol (Broncodil and trade) is a direct-acting sympathomimetic agent with mainly beta-adrenergic activity and a selective action on ß2 receptors (a ß2 agonist). It has properties similar to those of salbutamol. It is used as a bronchodilator in the management of reversible airways obstruction, as in asthma and in certain patients with chronic obstructive pulmonary disease. The uses, applications, and the synthetic pathways of this drug are outlined. Physical characteristics including: ionization constant, solubility, X-ray powder diffraction pattern, thermal methods of analysis, UV spectrum, IR spectrum, mass spectrum are all produced. This profile also includes the monograph of British Pharmacopoeia, together with several reported analytical methods including spectrophotometric, electrochemical, chromatographic, immunochemical methods, and capillary electrophoretic methods. The stability, the pharmacokinetic behavior, and the pharmacology of the drug are also provided.


Assuntos
Clembuterol , Agonistas Adrenérgicos beta/química , Agonistas Adrenérgicos beta/farmacocinética , Agonistas Adrenérgicos beta/farmacologia , Agonistas Adrenérgicos beta/uso terapêutico , Animais , Asma/tratamento farmacológico , Broncodilatadores/química , Broncodilatadores/farmacocinética , Broncodilatadores/farmacologia , Broncodilatadores/uso terapêutico , Clembuterol/química , Clembuterol/farmacocinética , Clembuterol/farmacologia , Clembuterol/uso terapêutico , Humanos , Estrutura Molecular , Doença Pulmonar Obstrutiva Crônica/tratamento farmacológico , Simpatomiméticos/química , Simpatomiméticos/farmacocinética , Simpatomiméticos/farmacologia , Simpatomiméticos/uso terapêutico
3.
Spectrochim Acta A Mol Biomol Spectrosc ; 107: 386-98, 2013 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-23485937

RESUMO

The experimental and theoretical vibrational spectra of S-S-2 methylamino-1-phenyl propan-1-ol (SSMPL). Fourier transform infrared (FTIR) and FT Raman spectra of SSMPL in the solid phase were recorded and analyzed. The molecular geometry, vibrational frequencies, infrared intensities, Raman activities and atomic charges were calculated using density functional theory calculation (B3LYP) with standard 6-31G(d,p) and high level 6-311++G(d,p) basis sets. Complete vibrational assignment and analysis of the fundamental modes of the compound were carried out using the observed FTIR and FT Raman data. The thermodynamic functions of the title compound were also performed by B3LYP with two basis sets 6-31G(d,p) and 6-311++G(d,p). Stability of the molecule arising from hyper conjugative interactions, charge delocalization has been analyzed using natural bond orbital (NBO) analysis. The calculated HOMO and LUMO energies show that charge transfer occurs within the molecule. Using the method B3LYP, the dipole moment (µ), polarizability (α) and the hyperpolarizability (ß) values of the investigated molecule has been computed. Total energy distribution (TED) was used for the assignment of Unambiguous vibrational fundamental modes. Finally, Simulated FTIR and FT Raman spectra of SSMPL showed good agreement with the observed spectra.


Assuntos
Metilaminas/química , Propanóis/química , Simpatomiméticos/química , Modelos Moleculares , Conformação Molecular , Teoria Quântica , Espectroscopia de Infravermelho com Transformada de Fourier , Análise Espectral Raman
4.
J AOAC Int ; 95(5): 1412-7, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-23175974

RESUMO

Spectrophotometric and stability-indicating HPLC procedures are described for determination of terbutaline sulfate in bulk powder and dosage form. The first procedure is based on diazo coupling of the phenolic groups of terbutaline sulfate with fast red B salt in the presence of sodium hydroxide. The colored compound developed in alkaline medium was measured at 475 nm. Different variables affecting the reaction were studied. Beer's Law is obeyed in the concentration range of 1-6 microg/mL. In the HPLC procedure, the separation was carried out on a Caltrex AIII column, a relatively new packing material consisting of silica-bonded calix[8]arene, using an isocratic binary mobile phase, acetonitrile-ammonium acetate (50 + 50, v/v), at pH 6.2. A diode array detector was used at 280 nm. The method was validated for system suitability, linearity, precision, LOD, LOQ, specificity, stability, and robustness. The LOD and LOQ were 0.196 and 0.781 microg/mL, respectively. The recovery values of this method were between 98 and 102%, and the reproducibility was within 0.92%. Statistical comparison of the results obtained from the analysis of the studied drug to those of the official British Pharmacopoeia (2007) method using t- and F-tests showed no significant difference between them.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Espectrofotometria/métodos , Simpatomiméticos/química , Terbutalina/química , Química Farmacêutica/métodos , Estrutura Molecular , Pós/química , Reprodutibilidade dos Testes , Sensibilidade e Especificidade
5.
J Fluoresc ; 22(6): 1461-74, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22752432

RESUMO

The absorption and fluorescence spectra of labetalol and pseudoephedrine have been studied in different polarities of solvents and ß-cyclodextrin (ß-CD). The inclusion complexation with ß-CD is investigated by UV-visible, steady state and time resolved fluorescence spectra and PM3 method. In protic solvents, the normal emission originates from a locally excited state and the longer wavelength emission is due to intramolecular charge transfer (TICT). Labetalol forms a 1:2 complex and pseudoephedrine forms 1:1 complex with ß-CD. Nanosecond time-resolved studies indicated that both molecules show triexponential decay. Thermodynamic parameters (ΔG, ΔH, ΔS) and HOMO, LUMO orbital investigations confirm the stability of the inclusion complex. The geometry of the most stable complex shows that the aromatic ring is deeply self included inside the ß-CD cavity and intermolecular hydrogen bonds were established between host and guest molecules. This suggests that hydrophobic effect and hydrogen bond play an important role in the inclusion process.


Assuntos
Anti-Hipertensivos/química , Portadores de Fármacos/química , Labetalol/química , Modelos Moleculares , Pseudoefedrina/química , Simpatomiméticos/química , beta-Ciclodextrinas/química , Cápsulas , Configuração de Carboidratos , Solventes/química , Espectrometria de Fluorescência
6.
Eur J Pharm Biopharm ; 82(3): 598-604, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22683694

RESUMO

Epinephrine is the drug of choice in the management of anaphylaxis. For first-aid treatment in the community, epinephrine autoinjectors (E-autos) are commonly prescribed, but are underutilized. In our laboratory, we developed a series of first-generation rapidly-disintegrating sublingual tablets (RDSTs) containing 40mg of epinephrine. One RDST had similar bioavailability to epinephrine 0.3mg from an auto-injector, as confirmed in a validated rabbit model, while other formulations containing different non-medicinal ingredients (NMIs) and with similar in vitro characteristics demonstrated much lower bioavailability. Subsequently, we evaluated the effect of changing the grade and proportion of NMIs, specifically mannitol and microcrystalline cellulose (MCC), on the in vitro characteristics of second- and third-generation RDSTs. Weight variation, content uniformity, breaking force, and friability were tested using official USP methods. Novel validated methods that simulate ambient conditions of the sublingual cavity were developed to test disintegration time, wetting time, and dissolution. Using these methods, it was possible to measure the effects of making small changes in NMIs on the in vitro characteristics of the formulations. The RDST formulation that resulted in the best in vitro characteristics contained the optimum proportion of mannitol and a specific ratio of coarse and fine particle grades of MCC. Appropriate comparative testing resulted in the selection of the RDST with the optimum in vitro characteristics.


Assuntos
Celulose/química , Epinefrina/administração & dosagem , Excipientes/química , Manitol/química , Administração Sublingual , Anafilaxia/tratamento farmacológico , Química Farmacêutica , Composição de Medicamentos , Epinefrina/química , Tamanho da Partícula , Solubilidade , Simpatomiméticos/administração & dosagem , Simpatomiméticos/química , Comprimidos , Fatores de Tempo
7.
J Sep Sci ; 35(8): 915-21, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22589151

RESUMO

The retention behavior for a series of biogenic amines and related sympathomimetic drugs has been investigated in reversed-phase thin-layer chromatography using RP-2, RP-8, RP-18W, and Diol stationary phase and mixtures of phosphate buffer (pH = 7.10) and methanol in different proportions as mobile phases. Several methodologies like arithmetic mean of experimental retention values, extrapolation to zero methanol concentration procedure and principal component analysis were applied to retention data values (R(M)) in order to determine relevant parameters (mean of R(M) - mR(M), R(M0), and scores corresponding to the first principal component - PC1/R(M) respectively) encoding information on the lipophilic behavior of compounds. High similarities in lipophilicity behavior of investigated amines were highlighted by mR(M) and PC1/R(M) lipophilicity indices for all of the studied stationary phases. The experimental results were compared with some computed lipophilicity parameters expressed as distribution coefficients at working pH (logD), partition coefficients (logP(N), logP(I), and diff(logP(N-I))) concerning both neutral and fully protonated species and difference between both species, and also with various lipophilicity values (logP) generated by different commonly used software. Significant correlations were observed between the experimental lipophilicity indices mR(M) respectively PC1/R(M) and diff(logP(N-I) ) values in all cases.


Assuntos
Aminas Biogênicas/química , Cromatografia em Camada Fina/métodos , Simpatomiméticos/química , Cromatografia em Camada Fina/instrumentação , Análise de Componente Principal , Software
8.
J Mater Sci Mater Med ; 22(6): 1465-77, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21553156

RESUMO

Naturally-occurring biomaterial scaffolds derived from extracellular matrix (ECM) have been previously investigated for soft tissue repair. We propose to enrich fascia ECM with high molecular weight tyramine substituted-hyaluronan (TS-HA) to modulate inflammation associated with implantation and enhance fibroblast infiltration. As critical determinants of constructive remodeling, the host inflammatory response and macrophage polarization to TS-HA enriched fascia were characterized in a rat abdominal wall model. TS-HA treated fascia with cross-linking had a similar lymphocyte (P = 0.11) and plasma cell (P = 0.13) densities, greater macrophage (P = 0.001) and giant cell (P < 0.0001) densities, and a lower density of fibroblast-like cells (P < 0.0001) than water treated controls. Treated fascia, with or without cross-linking, exhibited a predominantly M2 pro-remodeling macrophage profile similar to water controls (P = 0.82), which is suggestive of constructive tissue remodeling. Our findings demonstrated that HA augmentation can alter the host response to an ECM, but the appropriate concentration and molecular weight needed to minimize chronic inflammation within the scaffold remains to be determined.


Assuntos
Materiais Biocompatíveis/farmacologia , Fáscia/citologia , Ácido Hialurônico/química , Alicerces Teciduais/química , Tiramina/farmacologia , Parede Abdominal/fisiologia , Adolescente , Adulto , Animais , Materiais Biocompatíveis/química , Matriz Extracelular/química , Matriz Extracelular/efeitos dos fármacos , Fáscia/química , Fáscia/efeitos dos fármacos , Humanos , Ácido Hialurônico/farmacologia , Masculino , Pessoa de Meia-Idade , Ratos , Ratos Endogâmicos Lew , Simpatomiméticos/química , Simpatomiméticos/farmacologia , Tiramina/química , Adulto Jovem
9.
Drug Metab Dispos ; 39(4): 693-702, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21177487

RESUMO

Oxymetazoline (6-tert-butyl-3-(2-imidazolin-2-ylmethyl)-2,4-dimethylphenol) has been widely used as a nonprescription nasal vasoconstrictor for >40 years; however, its metabolic pathway has not been investigated. This study describes the in vitro metabolism of oxymetazoline in human, rat, and rabbit liver postmitochondrial supernatant fraction from homogenized tissue (S9) fractions and their microsomes supplemented with NADPH. The metabolites of oxymetazoline identified by liquid chromatography (LC)/UV/tandem mass spectrometry (MS/MS), included M1 (monohydroxylation of the t-butyl group), M2 (oxidative dehydrogenation of the imidazoline to an imidazole moiety), M3 (monohydroxylation of M2), M4 (dihydroxylation of oxymetazoline), and M5 (dihydroxylation of M2). Screening with nine human expressed cytochromes P450 (P450s) identified CYP2C19 as the single P450 isoform catalyzing the formation of M1, M2, and M3. Glutathione conjugates of oxymetazoline (M6) and M2 (M7) were identified in the liver S9 fractions, indicating the capability of oxymetazoline to undergo bioactivation to reactive intermediate species. M6 and M7 were not detected in those liver S9 incubations without NADPH. Cysteine conjugates (M8 and M9) derived from glutathione conjugates and hydroxylated glutathione conjugates (M10 and M11) were also identified. The reactive intermediate of oxymetazoline was trapped with glutathione and N-acetyl cysteine and identified by LC/MS/MS. M6 was isolated and identified by one-dimensional or two-dimensional NMR as the glutathione conjugate of a p-quinone methide. We have shown the tendency of oxymetazoline to form p-quinone methide species via a bioactivation mechanism involving a CYP2C19-catalyzed two-electron oxidation. Nevertheless, we conclude that the formation of this reactive species might not be a safety concern for oxymetazoline nasal products because of the typical low-dose and brief dosage regimen limited to nasal delivery.


Assuntos
Sistema Enzimático do Citocromo P-450/metabolismo , Glutationa/metabolismo , Oximetazolina/metabolismo , Simpatomiméticos/metabolismo , Acetilcisteína/metabolismo , Animais , Hidrocarboneto de Aril Hidroxilases/metabolismo , Citocromo P-450 CYP2C19 , Humanos , Hidroxilação , Técnicas In Vitro , Indolquinonas/metabolismo , Fígado/metabolismo , Masculino , Microssomos Hepáticos/metabolismo , NADP/metabolismo , Oxirredução , Oximetazolina/química , Coelhos , Ratos , Simpatomiméticos/química
10.
Biomed Chromatogr ; 24(10): 1125-9, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20853467

RESUMO

The development of methods for the separation of the enantiomers of fenoterol by chiral HPLC and capillary zone electrophoresis (CZE) is described. For the HPLC separation precolumn fluorescence derivatization with naphthyl isocyanate was applied. The resulting urea derivatives were resolved on a cellulose tris(3,5-dimethylphenylcarbamate)-coated silica gel column employing a column switching procedure. Detection was carried out fluorimetrically with a detection limit in the low ng/mL range. The method was adapted to the determination of fenoterol enantiomers in rat heart perfusates using liquid-liquid extraction. As an alternative a CE method was used for the direct separation of fenoterol enantiomers comparing different cyclodextrin derivatives as chiral selectors.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Eletroforese Capilar/métodos , Fenoterol/química , Agonistas de Receptores Adrenérgicos beta 2/química , Agonistas de Receptores Adrenérgicos beta 2/isolamento & purificação , Animais , Fenoterol/isolamento & purificação , Miocárdio/química , Ratos , Estereoisomerismo , Simpatomiméticos/química , Simpatomiméticos/isolamento & purificação
11.
J AOAC Int ; 93(1): 116-22, 2010.
Artigo em Inglês | MEDLINE | ID: mdl-20334173

RESUMO

A GC method was developed for the identification and quantitation of eight sympathomimetic amines in urine, i.e., amphetamine, methamphetamine, mephentermine, ephedrine, pseudoephedrine, methylenedioxyamphetamine, methylenedioxymethamphetamine, and methylenedioxyethylamphetamine. Methoxyphenamine was used as the internal standard (IS). The assay is rapid, sensitive, and simple to perform. It involves a liquid-liquid extraction procedure with simultaneous in-solution derivatization of the organic layer with pentafluorobenzoyl chloride (PFB-CI), followed by GC/MS analysis. These derivatives and the IS were extracted from 1 mL alkaline urine into hexane before derivatization with PFB-CI. The organic layer was then removed and evaporated to dryness before dissolution with hexane for GC/MS analysis. Calibration curves for each analyte showed linearity in the range of 25-5000 ng/mL (r2 > or = 0.997). Recoveries ranged from 88 to 99%, with the precision of recoveries typically < or = 5%. The LOD values ranged from 7 to 28 ng/mL, and the LOQ values ranged from 23 to 94 ng/mL. At least four ions were available for each analyte for confirmation of identity by MS.


Assuntos
Cromatografia Gasosa-Espectrometria de Massas/métodos , Simpatomiméticos/urina , 3,4-Metilenodioxianfetamina/análogos & derivados , 3,4-Metilenodioxianfetamina/urina , Anfetamina/urina , Efedrina/urina , Cromatografia Gasosa-Espectrometria de Massas/normas , Cromatografia Gasosa-Espectrometria de Massas/estatística & dados numéricos , Humanos , Mefentermina/urina , Metanfetamina/urina , Estrutura Molecular , N-Metil-3,4-Metilenodioxianfetamina/urina , Pseudoefedrina/urina , Padrões de Referência , Simpatomiméticos/química , Simpatomiméticos/normas
12.
Biomaterials ; 31(11): 3103-13, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-20116847

RESUMO

Polysaccharide hybrids consisting of hyaluronic acid (HA) grafted with a dextran-tyramine conjugate (Dex-TA) were synthesized and investigated as injectable biomimetic hydrogels for cartilage tissue engineering. The design of these hybrids (denoted as HA-g-Dex-TA) is based on the molecular structure of proteoglycans present in the extracellular matrix of native cartilage. Hydrogels of HA-g-Dex-TA were rapidly formed within 2 min via enzymatic crosslinking of the tyramine residues in the presence of horseradish peroxidase and hydrogen peroxide. The gelation time, equilibrium swelling and storage modulus could be adjusted by varying the degree of substitution of tyramine residues and polymer concentration. Bovine chondrocytes incorporated in the HA-g-Dex-TA hydrogels remained viable, as shown by the Live-dead assay. Moreover, enhanced chondrocyte proliferation and matrix production were observed in the HA-g-Dex-TA hydrogels compared to Dex-TA hydrogels. These results suggest that HA-g-Dex-TA hydrogels have a high potential as injectable scaffolds for cartilage tissue engineering.


Assuntos
Biomimética , Cartilagem/química , Dextranos/química , Ácido Hialurônico/química , Hidrogéis/química , Engenharia Tecidual , Animais , Materiais Biocompatíveis/química , Configuração de Carboidratos , Sequência de Carboidratos , Cartilagem/patologia , Bovinos , Proliferação de Células , Células Cultivadas , Condrócitos/química , Condrócitos/citologia , Reagentes de Ligações Cruzadas/química , Matriz Extracelular/química , Matriz Extracelular/metabolismo , Injeções , Teste de Materiais , Modelos Moleculares , Dados de Sequência Molecular , Simpatomiméticos/química , Engenharia Tecidual/instrumentação , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Tiramina/química
13.
J Pharm Biomed Anal ; 52(2): 203-9, 2010 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-20122809

RESUMO

The frequently used sympathomimetic drug phenylephrine has been studied by electrospray ionisation-mass spectrometry. The stability of the adrenoceptor agonist was examined by investigations of the pharmaceutically used salts phenylephrine hydrochloride and phenylephrine bitartrate. Photostability has been studied by use of an irradiation equipment emitting a solar radiation spectrum. The experiments were carried out by analysis of aqueous drug solutions before and after irradiation treatment. The phenylephrine derivative with unsaturated side chain originating from the drug by loss of one water molecule has been detected as the major degradation product of both phenylephrine salts the hydrochloride and the bitartrate. Further degradation and oxidation products were detectable already in the full scan mode demonstrating a low stability of the drug. Tandem mass spectrometry and multiple stage mass spectrometry experiments enabled the establishment of fragmentation schemes of both salts for the first time. Irradiation treatment indicated that phenylephrine bitartrate is more prone to degradation than the hydrochloride because of an additional decomposition sensitivity of the tartaric acid counter ion. An interaction between phenylephrine and its counter ion degradation products via a nucleophilic addition mechanism is suggested to be the explanation for the detected ion signals after irradiation treatment of phenylephrine bitartrate.


Assuntos
Fenilefrina/análise , Fenilefrina/química , Espectrometria de Massas por Ionização por Electrospray/métodos , Simpatomiméticos/análise , Simpatomiméticos/química , Estabilidade de Medicamentos , Espectrometria de Massas , Estrutura Molecular , Oxirredução , Processos Fotoquímicos/efeitos da radiação , Soluções , Espectrofotometria Ultravioleta , Espectrometria de Massas em Tandem , Água/química
14.
Pharmacol Ther ; 125(3): 363-75, 2010 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-19948186

RESUMO

Trace amines, including tyramine, beta-phenylethylamine (beta-PEA), tryptamine and octopamine, are biologically active amines mostly based on phenylethylamine, occurring in the body in trace amounts. They are a diverse group of naturally occurring and synthetic amines, which are also found in the diet and in herbal plants, such as ephedrine and cathinone. They include amphetamine and its analogues, such as MDMA ('ecstasy'), and synthetic proprietary sympathomimetic agents such as phenylpropanolamine and pseudoephedrine. On the vascular system they cause vasoconstriction and a rise in blood pressure. This effect is the basis of their use as nasal decongestants. For over 50 years, they have been assumed to be indirectly acting sympathomimetic amines, their responses being due to the release of noradrenaline from sympathetic neurones. There are, however, results that suggest that this is not their only mechanism of action and that they may also exert direct vascular effects independent of a noradrenergic mechanism. Recently, a group of novel trace amine-associated receptors (TAARs) have been cloned and identified in the brain and peripheral tissues including blood vessels. Trace amines bind to these cloned receptors and it is suggested that their vasoconstrictor effects can in part be attributed to this mechanism. This review describes the cardiovascular pharmacology of this diverse group of amines, their structures and uses and their endogenous synthesis and metabolism. The review also considers their clinical relevance as constituents of the diet, as therapeutic agents (ritodrine, phenylpropanolamine, and pseudoephedrine) and as drugs of abuse (amphetamine, 'ecstasy') and their mechanisms of action.


Assuntos
Sistema Cardiovascular/efeitos dos fármacos , Fenetilaminas/farmacologia , Receptores Acoplados a Proteínas G/metabolismo , Simpatomiméticos/farmacologia , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Doenças Cardiovasculares/induzido quimicamente , Sistema Cardiovascular/metabolismo , Humanos , Estrutura Molecular , Fenetilaminas/efeitos adversos , Fenetilaminas/química , Fenetilaminas/farmacocinética , Fenetilaminas/uso terapêutico , Receptores Acoplados a Proteínas G/efeitos dos fármacos , Simpatomiméticos/efeitos adversos , Simpatomiméticos/química , Simpatomiméticos/farmacocinética
15.
J Psychosoc Nurs Ment Health Serv ; 47(8): 17-21, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19681517

RESUMO

Chirality, the concept of nonsuperimposable mirror images, is a fundamental property of biological systems and can be observed on a molecular, cellular, or organism level. Stereoisomer compounds possess the same molecular and structural formula, but they differ in their three-dimensional configurations. Chiral compounds have two mirror-image stereoisomer forms called enantiomers. Compounds containing mirror-image enantiomers in equal proportions are referred to as racemic mixtures or racemates. Racemates and their individual enantiomers can have very different pharmacological properties that are relevant in clinical psychopharmacology. Various examples of drug therapies that show the clinical importance of chirality and stereochemistry are described.


Assuntos
Fármacos do Sistema Nervoso Central/química , Fármacos do Sistema Nervoso Central/farmacologia , Transtornos Mentais/tratamento farmacológico , Estereoisomerismo , Analgésicos Opioides/química , Analgésicos Opioides/farmacologia , Antidepressivos/química , Antidepressivos/farmacologia , Estimulantes do Sistema Nervoso Central/química , Estimulantes do Sistema Nervoso Central/farmacologia , Humanos , Psicofarmacologia , Simpatomiméticos/química , Simpatomiméticos/farmacologia
16.
Forensic Sci Int ; 185(1-3): e1-5, 2009 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-19157735

RESUMO

The urine specimens of numerous athletes were found to be positive for mephentermine both in-competition and out-of-competition in Taiwan. The donor of one specimen claimed she had only taken Mucaine (contains oxethazaine) for relieving symptomatic peptic ulcer and gastritis. Oxethazaine is not included in the prohibited list of the World Anti-Doping Agency; however, its metabolized compounds, mephentermine and phentermine, are included in that list. This study applied LC-MS-MS to analyze the excretions of three volunteers who ingested oxethazaine and presented positive results for mephentermine and/or phentermine. Thus, oxethazaine is the source of mephentermine and phentermine. Moreover, the results showed that 48 brands of gastric medicines containing oxethazaine were legally imported or locally manufactured in Taiwan, information which could be useful for limiting the misuse of oxethazaine by athletes. The data suggested that the sports associations should warn athletes about the risks of taking oxethazaine.


Assuntos
Antiácidos/química , Dopagem Esportivo , Etanolaminas/química , Mefentermina/urina , Fentermina/urina , Antiácidos/administração & dosagem , Antiácidos/farmacocinética , Estimulantes do Sistema Nervoso Central/química , Estimulantes do Sistema Nervoso Central/urina , Cromatografia Líquida , Etanolaminas/farmacocinética , Feminino , Humanos , Espectrometria de Massas , Mefentermina/química , Estrutura Molecular , Fentermina/química , Simpatomiméticos/química , Simpatomiméticos/urina , Taiwan
17.
Amino Acids ; 36(2): 185-93, 2009 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-18317872

RESUMO

The synthesis, isolation, spectroscopic and structural elucidation of sympathomimetic amine, tyramine dihydrogenphosphate are of interest due to its biological activity and the establishing correlation between spectroscopic properties and structure. The complex approach for investigation included single crystal X-ray diffraction, new technique in linear-polarized IR-spectroscopy in solid state and quantum chemical calculations with a view to predict the electronic structure and vibrational data of interacting species in entitled compound, the correlation structure-spectroscopic properties as well as the influence of intermolecular interaction on IR-characteristic bands are carried out.


Assuntos
Simpatomiméticos/química , Tiramina/química , Cristalização , Cristalografia por Raios X , Modelos Químicos , Estrutura Molecular , Espectrofotometria Infravermelho , Simpatomiméticos/síntese química , Tiramina/síntese química
18.
J Psychopharmacol ; 23(1): 51-5, 2009 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-18562433

RESUMO

In human subjects, the acute tryptophan (TRP) depletion (ATD) paradigm has been shown to have effects on mood and cognition. It is assumed that these effects are mediated through the serotonin system. In this study, we have examined the effects of ATD on the central concentrations of the monoamine transmitters, noradrenaline (NA) and dopamine (DA) as well as on serotonin (5-HT). Effects on NA and DA could also affect mood and cognition. Following oral administration of TRP-containing (TRP+) and TRP-free (TRP-) amino acid mixtures, neurotransmitter concentrations and free plasma TRP concentrations were determined by High Performance Liquid Chromatography (HPLC) with electrochemical detection. Free plasma TRP was significantly and substantially reduced (79%) in rats given a TRP- amino acid mixture when compared with those given a TRP+ mixture. ATD also significantly decreased 5-HT and 5-hydroxyindolacetic acid in the frontal cortex, remaining cortex and hippocampus, but did not significantly reduce these in the striatum. Furthermore, ATD did not significantly alter the concentration of NA and DA in any brain region examined. This study demonstrates that the administration of a TRP- amino acid mixture in rats can reduce free plasma TRP to levels comparable to those reported in human studies. These results indicate that behavioural and cognitive changes produced by ATD in preclinical or clinical studies are likely to be due to specific effects on the serotonergic system.


Assuntos
Aminoácidos Essenciais/deficiência , Química Encefálica/efeitos dos fármacos , Dopamina/metabolismo , Norepinefrina/metabolismo , Simpatomiméticos/metabolismo , Triptofano/deficiência , Triptofano/farmacologia , Ácido 3,4-Di-Hidroxifenilacético/análise , Ácido 3,4-Di-Hidroxifenilacético/química , Ácido 3,4-Di-Hidroxifenilacético/metabolismo , Aminoácidos Essenciais/sangue , Aminoácidos Essenciais/química , Aminoácidos Essenciais/farmacologia , Animais , Peso Corporal/fisiologia , Corpo Estriado/química , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/metabolismo , Suplementos Nutricionais , Dopamina/análise , Lobo Frontal/química , Lobo Frontal/efeitos dos fármacos , Lobo Frontal/metabolismo , Hipocampo/química , Hipocampo/efeitos dos fármacos , Hipocampo/metabolismo , Ácido Homovanílico/química , Ácido Homovanílico/metabolismo , Humanos , Ácido Hidroxi-Indolacético/análise , Ácido Hidroxi-Indolacético/antagonistas & inibidores , Ácido Hidroxi-Indolacético/química , Masculino , Norepinefrina/análise , Ratos , Ratos Sprague-Dawley , Serotonina/análise , Serotonina/deficiência , Simpatomiméticos/análise , Simpatomiméticos/química , Triptofano/sangue , Triptofano/química
19.
Psychopharmacology (Berl) ; 201(3): 413-22, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18766330

RESUMO

RATIONALE: Somatostatin and its receptors have been localized in brain nuclei implicated in motor control, such as the striatum, nucleus accumbens, ventral pallidum, and globus pallidus (GP). OBJECTIVES: The objective of this study was to investigate the role of somatostatin receptors (sst(1,2,4)) in the GP on dopamine (DA)-mediated behaviors, such as locomotor activity, and to examine the GP-striatum circuitry by correlating the effect of somatostatin in the GP with the release of DA in the striatum. MATERIALS AND METHODS: Animals received saline, somatostatin (60, 120, 240 ng/0.5 microl per side) or the following selective ligands: L-797,591 (sst(1) analog, 60, 120, 240 ng/0.5 microl per side), L-779,976 (sst(2) analog, 120, 240, 480 ng/0.5 microl per side), L-803,087 (sst(4) analog; 120, 240, 480 ng/0.5 microl per side), L-796,778 (sst(3) analog, 240 ng/0.5 microl per side), SRA-880 (sst(1) selective antagonist + somatostatin, 120 ng/0.5 microl per side), CYN154806 (sst(2) selective antagonist + somatostatin, 120 ng/0.5 microl per side) bilaterally in the GP of the rat. Locomotor activity was measured for 60 min. The effect of somatostatin, administered intrapallidally, on the extracellular concentrations of DA, 3,4-dihydroxyphenylacetic acid, and homovanillic acid in the striatum was also studied in the behaving rat using in vivo microdialysis methodology. RESULTS: Somatostatin increased the locomotor activity of the rat in a dose-dependent manner. This effect was mediated by activation of the sst(1), sst(2), and sst(4) receptors. Selective sst agonists increased locomotor activity in a statistical significant manner, while selective sst(1) and sst(2) antagonists reversed the somatostatin-mediated locomotor activity to control levels. DA levels increased in the striatum after intrapallidal infusion of somatostatin (240 ng/side). CONCLUSIONS: These data provide behavioral and neurochemical evidence of the functional role of somatostatin receptors in the GP-striatum circuitry.


Assuntos
Corpo Estriado/efeitos dos fármacos , Corpo Estriado/metabolismo , Dopamina/metabolismo , Globo Pálido/efeitos dos fármacos , Atividade Motora/efeitos dos fármacos , Receptores de Somatostatina/antagonistas & inibidores , Receptores de Somatostatina/fisiologia , Simpatomiméticos/metabolismo , Amidas/farmacologia , Animais , Corpo Estriado/química , Dopamina/análise , Dopamina/química , Relação Dose-Resposta a Droga , Globo Pálido/fisiologia , Indóis/farmacologia , Microdiálise/métodos , Modelos Anatômicos , Atividade Motora/fisiologia , Naftalenos/farmacologia , Neuropeptídeos/química , Neuropeptídeos/farmacologia , Nitrobenzenos/farmacologia , Oligopeptídeos/farmacologia , Piperazinas/farmacologia , Piridinas/farmacologia , Quinolinas/farmacologia , Ratos , Receptores de Somatostatina/agonistas , Cloreto de Sódio/administração & dosagem , Somatostatina/química , Somatostatina/farmacologia , Simpatomiméticos/química
20.
J Sep Sci ; 31(18): 3212-7, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18773417

RESUMO

Liquid-liquid-liquid microextraction (LLLME) in combination with HPLC and UV detection has been used as a sensitive method for the determination of ephedrine in urine samples. Extraction process was performed in a homemade total glass vial without using a Teflon ring, usually employed. Ephedrine was first extracted from 3.5 mL of urine sample (pH 12) into a microfilm of toluene/benzene (50:50). The analyte was subsequently back extracted into an acidic microdrop solution (pH 2) suspended in the organic phase. The extract was then injected into the HPLC system directly. An enrichment factor of 137 along with a good sample clean-up was obtained under the optimized conditions. The calibration curve showed linearity in the range of 0.01-50 mg/L with regression coefficient corresponding to 0.998. The LODs and LOQs, based on a S/N of 3 and 10, were 5 and 10 microg/L, respectively. The method was eventually applied for the determination of ephedrine in urine sample after oral administration of 5 mg single dose of drug.


Assuntos
Fracionamento Químico/métodos , Cromatografia Líquida de Alta Pressão/métodos , Efedrina/urina , Simpatomiméticos/urina , Fracionamento Químico/instrumentação , Cromatografia Líquida de Alta Pressão/instrumentação , Dopagem Esportivo , Efedrina/química , Humanos , Estrutura Molecular , Reprodutibilidade dos Testes , Solventes/química , Simpatomiméticos/química
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