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1.
Syst Parasitol ; 101(5): 54, 2024 Aug 09.
Artigo em Inglês | MEDLINE | ID: mdl-39120762

RESUMO

Ergasilus lizae Krøyer, 1863 is a parasitic copepod known to infect mullets (Mugilidae) in different parts of the world. It was originally reported from the east coast of North America, but the original description lacks enough detail, making identification with this information difficult. In this study, we provide a redescription of E. lizae found on Mugil curema Valenciennes and M. cephalus Linnaeus, caught in two coastal lagoons of northwestern Mexico during two climatic seasons: warm/rainy and cold/dry. The prevalence of this parasite was higher in the warm season than in the cold season. To facilitate the species identification, new sequences of the barcoding gene (COI mtDNA) of E. lizae were generated and compared against unpublished sequences of E. lizae available in the Barcode of Life Database (BOLD). Our results suggest that the sequences of BOLD possibly belong to a species misidentified as E. lizae.


Assuntos
Copépodes , Código de Barras de DNA Taxonômico , Estações do Ano , Especificidade da Espécie , Animais , Copépodes/genética , Copépodes/anatomia & histologia , Copépodes/classificação , México , Smegmamorpha/parasitologia , Filogenia , Complexo IV da Cadeia de Transporte de Elétrons/genética
2.
J Parasitol ; 110(4): 393-401, 2024 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-39169851

RESUMO

Myxospores discovered floating free in the bile of marine fishes from the south-central coast of Vietnam were identified using morphological and molecular methods, leading to the description of 2 new species. Ceratomyxa chauvanminhi n. sp. was detected in 16% (8/50) of cultured barramundi Lates calcarifer (Bloch) specimens, and Ceratomyxa sekoi n. sp. was found in 20% (5/25) of wild largehead hairtail Trichiurus lepturus Linnaeus specimens. The spores of C. chauvanminhi n. sp. are very shallowly ovoid, slightly crescent shaped, and 11.5 ± 0.5 (10.7-12.4) µm thick, 5.8 ± 0.2 (5.4-6.1) µm long, and 5.5 ± 0.2 (5.2-5.7) µm wide. Their posterior angles are slightly concave at 158.7° ± 4.2° (151.3°-164.8°), and they possess 2 equal spherical polar capsules 2.5 ± 0.2 (2.1-2.9) µm in diameter. The spores of C. sekoi n. sp. are 5.6 ± 0.2 (5.0-6.1) µm long, 75.5 ± 4.8 (68.9-90.0) µm thick, and 5.5 ± 0.1 (5.4-5.6) µm wide, with 2 equal, slightly anterior spherical polar capsules 2.1 ± 0.2 (1.7-2.4) µm in diameter. Although C. sekoi n. sp. spores resemble those of species of MyxodavisiaZhao, Zhou, Kent, and Whipps, 2008, characterized by long tapering valves, genetic analyses distinctly place this new species within the Ceratomyxa Thélohan, 1892 lineage. This study contributes to the understanding myxosporean diversity in Vietnamese waters and highlights the difficulty associated with distinguishing between the genera Ceratomyxa and Myxodavisia.


Assuntos
Doenças dos Peixes , Vesícula Biliar , Myxozoa , Doenças Parasitárias em Animais , Perciformes , Filogenia , Animais , Vietnã , Doenças dos Peixes/parasitologia , Doenças dos Peixes/epidemiologia , Myxozoa/classificação , Myxozoa/isolamento & purificação , Myxozoa/genética , Myxozoa/anatomia & histologia , Vesícula Biliar/parasitologia , Perciformes/parasitologia , Doenças Parasitárias em Animais/parasitologia , Doenças Parasitárias em Animais/epidemiologia , Doenças da Vesícula Biliar/parasitologia , Doenças da Vesícula Biliar/veterinária , Peixes/parasitologia , Prevalência , DNA Ribossômico/química , Smegmamorpha/parasitologia
3.
Parasitol Int ; 102: 102918, 2024 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-38945391

RESUMO

The coastal waters of Vietnam are home to a wide diversity of fishes, but the parasite diversity of these potential hosts is much less well characterized. To begin addressing this knowledge gap, we carried out surveys of myxozoan parasites in fishes collected from Nha Trang Bay in Vietnam's East Sea in 2018-2019. Mugilid fishes were collected in March-April 2018, January-February 2019, and November-December 2019, and examined for myxozoans. Myxospores consistent with those of the genus Ellipsomyxa were found in the gall bladder of four mullet species, and we thoroughly characterized those from Planiliza melinoptera. Myxospores were elliptoid and devoid of striation, with a distinct sinuous suture line. Polar capsules were pyriform and oriented toward the poles of the spore. Morphological features were compared to nominal species and this species from Vietnam was distinct. Phylogenetic analysis based on partial small subunit rDNA sequence revealed that broadly, Ellipsomyxa species split into three phylogenetic lineages, and although in some branches there are groupings by host family, habitat or locality, there are no clear phylogenetic patterns. The new species we encountered in P. melinoptera had a close sister relationship with Ellipsomyxa adlardi, with both species part of a larger subclade within the Ellipsomyxa lineage. Despite this phylogenetic similarity, these species were morphologically distinct, and partial large subunit DNA sequences were only 93% similar to each other. A combination of the morphological characteristics and molecular data suggest that this is an undescribed species and we propose the name Ellipsomyxa gordeyi n. sp.


Assuntos
Doenças dos Peixes , Vesícula Biliar , Myxozoa , Filogenia , Smegmamorpha , Animais , Vietnã , Doenças dos Peixes/parasitologia , Vesícula Biliar/parasitologia , Smegmamorpha/parasitologia , Myxozoa/classificação , Myxozoa/genética , Myxozoa/anatomia & histologia , Myxozoa/isolamento & purificação , Doenças Parasitárias em Animais/parasitologia , Doenças Parasitárias em Animais/epidemiologia , Baías
4.
Fish Shellfish Immunol ; 149: 109580, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38663464

RESUMO

Wild organisms are regularly exposed to a wide range of parasites, requiring the management of an effective immune response while avoiding immunopathology. Currently, our knowledge of immunoparasitology primarily derives from controlled laboratory studies, neglecting the genetic and environmental diversity that contribute to immune phenotypes observed in wild populations. To gain insight into the immunologic variability in natural settings, we examined differences in immune gene expression of two Alaskan stickleback (Gasterosteus aculeatus) populations with varying susceptibility to infection by the cestode Schistocephalus solidus. Between these two populations, we found distinct immune gene expression patterns at the population level in response to infection with fish from the high-infection population displaying signs of parasite-driven immune manipulation. Further, we found significant differences in baseline immune gene profiles between the populations, with uninfected low-infection population fish showing signatures of inflammation compared to uninfected high-infection population fish. These results shed light on divergent responses of wild populations to the same parasite, providing valuable insights into host-parasite interactions in natural ecosystems.


Assuntos
Cestoides , Infecções por Cestoides , Doenças dos Peixes , Smegmamorpha , Animais , Smegmamorpha/imunologia , Smegmamorpha/genética , Smegmamorpha/parasitologia , Doenças dos Peixes/imunologia , Doenças dos Peixes/parasitologia , Infecções por Cestoides/veterinária , Infecções por Cestoides/imunologia , Infecções por Cestoides/parasitologia , Cestoides/imunologia , Cestoides/fisiologia , Interações Hospedeiro-Parasita/imunologia , Alaska , Imunidade Inata/genética
5.
J Parasitol ; 109(4): 419-422, 2023 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-37580060

RESUMO

A survey of threespine stickleback (Gasterosteus aculeatus L.) and juvenile Chinook salmon (Oncorhynchus tshawytscha (Walbaum)) from the lower Fraser River, British Columbia, Canada and adjacent marine waters was conducted for the presence of myxozoan parasites of the genus Ceratonova Atkinson, Foott, and Bartholomew, 2014 (Bivalvulida, Ceratomyxidae). Quantitative polymerase chain reaction (PCR; small subunit ribosomal RNA gene) combined with conventional PCR and sequencing (ribosomal internal transcribed spacer region 1) detected Ceratonova gasterostea Atkinson, Foott, and Bartholomew, 2014 in 89 of 269 (33.1%) sticklebacks from the Fraser River and in 1 of 51 (2%) from Howe Sound. In contrast, Ceratonova shasta (Atkinson, Foott, and Bartholomew, 2014) was detected in 2 of 26 (7.7%) Chinook salmon from the Fraser River. Reciprocal infections were not detected. Light microscopic examination of the stickleback intestine revealed the presence of an intense infection with large numbers of pseudoplasmodia which interacted with enterocytes through pseudopodia-like projections. Myxospores with characteristic elongate valve cells were visible within mature pseudoplasmodia and free in the intestinal lumen. This is the first report of C. gasterostea in Canada.


Assuntos
Cnidários , Doenças dos Peixes , Myxozoa , Smegmamorpha , Animais , Colúmbia Britânica/epidemiologia , Myxozoa/genética , Salmão/parasitologia , Smegmamorpha/parasitologia , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia
6.
Sci Rep ; 13(1): 1054, 2023 01 19.
Artigo em Inglês | MEDLINE | ID: mdl-36658208

RESUMO

Stable isotope analysis of individual compounds is emerging as a powerful tool to study nutrient origin and conversion in host-parasite systems. We measured the carbon isotope composition of amino acids and glucose in the cestode Schistocephalus solidus and in liver and muscle tissues of its second intermediate host, the three-spined stickleback (Gasterosteus aculeatus), over the course of 90 days in a controlled infection experiment. Similar linear regressions of δ13C values over time and low trophic fractionation of essential amino acids indicate that the parasite assimilates nutrients from sources closely connected to the liver metabolism of its host. Biosynthesis of glucose in the parasite might occur from the glucogenic precursors alanine, asparagine and glutamine and with an isotope fractionation of - 2 to - 3 ‰ from enzymatic reactions, while trophic fractionation of glycine, serine and threonine could be interpreted as extensive nutrient conversion to fuel parasitic growth through one-carbon metabolism. Trophic fractionation of amino acids between sticklebacks and their diets was slightly increased in infected compared to uninfected individuals, which could be caused by increased (immune-) metabolic activities due to parasitic infection. Our results show that compound-specific stable isotope analysis has unique opportunities to study host and parasite physiology.


Assuntos
Cestoides , Infecções por Cestoides , Doenças dos Peixes , Parasitos , Smegmamorpha , Animais , Humanos , Infecções por Cestoides/parasitologia , Isótopos de Carbono , Carbono , Aminoácidos , Cestoides/fisiologia , Smegmamorpha/parasitologia , Nutrientes , Interações Hospedeiro-Parasita , Doenças dos Peixes/parasitologia
7.
J Helminthol ; 96: e85, 2022 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-36377340

RESUMO

Ligophorus kaohsianghsieni (Gusev, ) Gusev, was collected from the so-iuy mullet Planiliza haematocheilus (Temminck & Schlegel, 1845) from the Black Sea and the Sea of Japan. DNA sequences data for L. kaohsianghsieni, as well as its morphological characters from the Sea of Japan were obtained for the first time. Significant morphometric and genetic diversity between specimens of L. kaohsianghsieni from the Black-Azov Sea region and the Sea of Japan were not found. For the first time, the molecular phylogeny of L. kaohsianghsieni based on three fragments of the nuclear DNA ribosomal cluster (18S, internal transcribed spacer 1 and 28S) was reconstructed. Molecular analysis of Ligophorus species from the Atlantic and Pacific Oceans revealed a significant phylogenetic distance between L. kaohsianghsieni and two others species (Ligophorus pilengas and Ligophorus llewellyni) from the same host (P. haematocheilus) and region. This result indicates the lack of correspondence between the phylogenetic and geographical closeness of the hosts and the relation of their parasites from the genus Ligophorus.


Assuntos
Platelmintos , Smegmamorpha , Trematódeos , Animais , Filogenia , Mar Negro , Japão , Trematódeos/genética , Smegmamorpha/parasitologia
8.
J Helminthol ; 96: e70, 2022 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-36148561

RESUMO

Plerocerci of the trypanorhynch cestode Progrillotia dasyatidis Beveridge, Neifar & Euzet, (Progrillotiidae) were isolated from the gallbladder of the three-spined stickleback Gasterosteus aculeatus from a small freshwater marsh in the Lake Atanasovsko Wetlands, a coastal area adjacent to the Black Sea coast, Bulgaria. The parasite was recorded in five out of 134 fish individuals studied (prevalence 3.73%, intensity 1-7, mean intensity 2.40 ± 1.17 and mean abundance 0.09 ± 0.06). A description of the plerocerci is presented, expanding data on intraspecific variation. The present report is the first record of P. dasyatidis from G. aculeatus (new host record) and from Bulgaria (new geographical record). Recording only plerocerci with evaginated scoleces in the sticklebacks is consistent with the hypothesis that teleosts are paratenic hosts and not intermediate hosts of P. dasyatidis.


Assuntos
Cestoides , Infecções por Cestoides , Doenças dos Peixes , Parasitos , Smegmamorpha , Animais , Bulgária/epidemiologia , Infecções por Cestoides/epidemiologia , Infecções por Cestoides/parasitologia , Infecções por Cestoides/veterinária , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Lagos , Estágios do Ciclo de Vida , Smegmamorpha/parasitologia , Áreas Alagadas
9.
Exp Parasitol ; 240: 108335, 2022 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-35932907

RESUMO

Parasite identification is crucial in areas where no sanitary inspection is conducted on fish, especially considering that parasitic zoonoses like anisakiasis and gnathostomiasis can pose a risk for human health. In this study, parasites in mullet fish (Mugil curema) from the Chautengo Lagoon, Guerrero, Mexico, were identified by morphological and molecular methods. A total of 122 specimens weighing 317 ± 51.25 g and 19.3 ± 1.14 cm in length were assessed. Their helminthofauna was classified by measuring internal structures, total length, and maximum width; a morphometric index was also calculated for larval stages. The prevalence of parasitosis in these mullets was 91.8%, with a mean infection intensity of 4.1. The acanthocephalan Floridosentis mugilis was identified by its external and internal structures. The nematodes found were of the Anisakidae family in stage 3 (L3), with a morphology consistent with Contracaecum sp. To determine the species, the ITS ribosomal gene and the mitochondrial genes COX2 and rrnS were molecularly characterized by PCR; then, they were aligned by CLUSTAL W, and a phylogenetic tree was obtained. In this analysis, the sequences were compared with those reported in GenBank. A total of 460 parasites were studied, 283 of which were nematodes (61.5%) and 177 were acanthocephalans (38.5%). The sequences of seven nematodes showed 99% homology with each other, and thus they formed an independent branch within the Contracaecum sp. group. This is the first report identifying Contracaecum multipapillatum in mullet fish in the Chautengo Lagoon, Guerrero.


Assuntos
Ascaridoidea , Doenças dos Peixes , Parasitos , Smegmamorpha , Animais , Ascaridoidea/genética , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Peixes/parasitologia , Humanos , México/epidemiologia , Filogenia , Smegmamorpha/parasitologia
10.
Ann Parasitol ; 68(2): 353-365, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35810385

RESUMO

The leaping mullet Chelon saliens is one of the economically significant fish species and the revealing its parasite fauna in relation with some ecological and host related factors will provide new data for our current knowledge. A total of 165 leaping mullet were collected from Sinop coasts of the Black Sea in the period from September 2015 to August 2016 and investigated for parasites. Eleven parasite species including Myxobolus parvus, Myxobolus sp., Sphaerospora mugilis (Myxozoa), Ligophorus szidati, Solostamenides mugilis (Monogenea), Schikhobalotrema sparisomae, Saccocoelium tensum, Saccocoelium obesum (Digenea), Hysterothylacium aduncum (Nematoda), Neoechinorhynchus sp. (Acanthocephala) and Ergasilus lizae (Copepoda) have been identified. The overall infection prevalence, mean intensity and mean abundance values were 65.5%, 26.2 and 17.2, respectively. The overall infection prevalence was dominated by L. szidati, followed by M. parvus and Digenea-group. On the other hand, the overall mean intensity values were dominated by Digenea-group, followed by L. szidati and E. lizae, respectively, while the mean abundance values were dominated by L. szidati, followed by Digenea-group and E. lizae. The infection indices of all identified parasites were also calculated in relation with length classes and sex of fish as well as season and the differences were evaluated statistically. Seasonally significant differences in the infection prevalence and mean abundance were found for Digenea-group, Ligophorus szidati and Neoechinorhynchus sp. These differences were also significant in the length classes of Digenea-group and Ligophorus szidati. This study is the first investigation on seasonal and host related dynamics of parasites of C. saliens in the southern coasts of the Black Sea and all investigated factors were found to influence the infection indices of dominating parasite species.


Assuntos
Copépodes , Doenças dos Peixes , Myxobolus , Parasitos , Smegmamorpha , Trematódeos , Animais , Mar Negro , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Peixes , Cimento de Policarboxilato , Estações do Ano , Smegmamorpha/parasitologia
11.
Sci Rep ; 12(1): 10049, 2022 06 16.
Artigo em Inglês | MEDLINE | ID: mdl-35710685

RESUMO

Mullets (Mugilidae) are economically important fish in Israel. Two species of mugilids (i.e., the thinlip mullet Chelon ramada and the flathead grey mullet Mugil cephalus) have been stocked in the Sea of Galilee (Lake Kinneret) in order to increase fishermen's income and lake water quality. These catadromous species do not reproduce in the lake, consequently, fingerlings have been introduced every year since 1958. Following a survey of myxozoan infections in the Sea of Galilee, we described Myxobolus pupkoi n. sp. infecting the gill arches, and reported Myxobolus exiguus from visceral peritoneum and gall bladder of C. ramada. The prevalence of infection of both Myxobolus pupkoi n. sp. and M. exiguus were 11.5% (2/23). Our study indicates that the parasites infecting C. ramada belong to a lineage of myxozoans infecting mugilids. This result suggests that the infection took place in the Mediterranean Sea, where the fingerlings were caught, before their introduction into the Sea of Galilee. Since 2018 only farm-raised fingerlings have been introduced. We thus recommend to closely monitor the presence of these parasites in the future to determine if the presence of parasites disappear with the introduction of farm-raised fingerlings.


Assuntos
Doenças dos Peixes , Myxobolus , Smegmamorpha , Animais , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Filogenia , Cimento de Policarboxilato , Smegmamorpha/parasitologia
12.
Parasitology ; 149(9): 1173-1178, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35570667

RESUMO

The cestode Schistocephalus solidus is a common parasite in freshwater threespine stickleback populations, imposing strong fitness costs on their hosts. Given this, it is surprising how little is known about the timing and development of infections in natural stickleback populations. Previous work showed that young-of-year stickleback can get infected shortly after hatching. We extended this observation by comparing infection prevalence of young-of-year stickleback from 3 Alaskan populations (Walby, Cornelius and Wolf lakes) over 2 successive cohorts (2018/19 and 2019/20). We observed strong variation between sampling years (2018 vs 2019 vs 2020), stickleback age groups (young-of-year vs 1-year-old) and sampling populations.


Assuntos
Infecções por Cestoides , Doenças dos Peixes , Smegmamorpha , Alaska/epidemiologia , Animais , Infecções por Cestoides/epidemiologia , Infecções por Cestoides/veterinária , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Interações Hospedeiro-Parasita , Lagos/parasitologia , Smegmamorpha/parasitologia
13.
Parasitology ; 149(9): 1164-1172, 2022 08.
Artigo em Inglês | MEDLINE | ID: mdl-35570701

RESUMO

How parasites alter host feeding ecology remains elusive in natural populations. A powerful approach to investigate the link between infection and feeding ecology is quantifying unique and shared responses to parasite infection in related host species within a common environment. Here, 9 pairs of sympatric populations of the three-spined and nine-spined stickleback fishes were sampled across a range of freshwater and brackish habitats to investigate how parasites alter host feeding ecology: (i) biotic and abiotic determinants of parasite community composition, and (ii) to what extent parasite infection correlates with trophic niche specialization of the 2 species, using stable isotope analyses (δ15N and δ13C). It was determined that parasite community composition and host parasite load varied among sites and species and were correlated with dissolved oxygen. It was also observed that the digenean Cyathocotyle sp.'s abundance, a common directly infecting parasite with a complex life cycle, correlated with host δ13C in a fish species-specific manner. In 6 sites, correlations were found between parasite abundance and their hosts' feeding ecology. These effects were location-specific and occasionally host species or host size-specific. Overall, the results suggest a relationship between parasite infection and host trophic niche which may be an important and largely overlooked ecological factor. The population specificity and variation in parasite communities also suggest this effect is multifarious and context-dependent.


Assuntos
Doenças dos Peixes , Doenças Parasitárias , Smegmamorpha , Trematódeos , Animais , Doenças dos Peixes/parasitologia , Peixes , Interações Hospedeiro-Parasita , Smegmamorpha/parasitologia
14.
Parasitol Res ; 121(6): 1607-1619, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35435510

RESUMO

Since 2012, a massive invasion of the three-spined stickleback (Gasterosteus aculeatus) has taken place into the pelagic area of Lake Constance. This species, which had previously been restricted to the littoral zone, is now the dominant pelagic fish and the previously dominant whitefish (Coregonus wartmanni) has suffered severe reductions in growth and recruitment. In this study, in total, 2871 sticklebacks were collected via monthly sessions over a 4-year period in pelagic and benthic areas of Lake Constance and examined for signs of infection with Schistocephalus solidus, a parasite known to be potentially fatal. The infection risk to sticklebacks increases throughout the course of the year and is size- and sex-dependent. Habitat has only a marginal impact. All parasite-induced harm is imparted after stickleback spawning and parental care is over. The results did not support the hypothesis that the invasion of the pelagic area might be driven by parasite-avoiding behaviour. Furthermore, the impact of the parasite is likely to be limited to post-reproductive adults, thereby ensuring stable reproduction of the hosts despite high rates of transmission and mortality. In consequence, stickleback stock development is independent of S. solidus infection, leading to secure coexistence of host and parasite even at extraordinary high host levels.


Assuntos
Cestoides , Infecções por Cestoides , Doenças dos Peixes , Parasitos , Salmonidae , Smegmamorpha , Animais , Infecções por Cestoides/parasitologia , Doenças dos Peixes/epidemiologia , Doenças dos Peixes/parasitologia , Interações Hospedeiro-Parasita , Smegmamorpha/parasitologia
15.
Parasitol Res ; 121(5): 1305-1315, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-35307765

RESUMO

Trophically transmitted parasites have life cycles that require the infected host to be eaten by the correct type of predator. Such parasites should benefit from an ability to suppress the host's fear of predators, but if the manipulation is imprecise the consequence may be increased predation by non-hosts, to the detriment of the parasite. Three-spined sticklebacks (Gasterosteus aculeatus) infected by the cestode Schistocephalus solidus express reduced antipredator behaviours, but it is unknown whether this is an example of a highly precise manipulation, a more general manipulation, or if it can even be attributed to mere side effects of disease. In a series of experiments, we investigated several behaviours of infected and uninfected sticklebacks. As expected, they had weak responses to simulated predatory attacks compared to uninfected fish. However, our results suggest that the parasite induced a general fearlessness, rather than a precise manipulation aimed at the correct predators (birds). Infected fish had reduced responses also when attacked from the side and when exposed to odour from a fish predator, which is a "dead-end" for this parasite. We also tested whether the reduced anti-predator behaviours were mere symptoms of a decreased overall vigour, or due to parasite-induced hunger, but we found no support for these ideas. We propose that even imprecise manipulations of anti-predator behaviours may benefit parasites, for example, if other behaviours are altered in a way that increases the exposure to the correct predator.


Assuntos
Cestoides , Infecções por Cestoides , Doenças dos Peixes , Parasitos , Smegmamorpha , Animais , Cestoides/fisiologia , Infecções por Cestoides/parasitologia , Infecções por Cestoides/veterinária , Doenças dos Peixes/parasitologia , Peixes , Interações Hospedeiro-Parasita , Smegmamorpha/parasitologia
16.
Mol Ecol ; 31(5): 1577-1594, 2022 03.
Artigo em Inglês | MEDLINE | ID: mdl-35000227

RESUMO

A growing literature demonstrates the impact of helminths on their host gut microbiome. We investigated whether the stickleback host microbiome depends on ecoevolutionary variables by testing the impact of exposure to the cestode parasite Schistocephalus solidus with respect to infection success, host genotype, parasite genotype, and parasite microbiome composition. We observed constitutive differences in the microbiome of sticklebacks of different origin, and those differences increased when sticklebacks exposed to the parasite resisted infection. In contrast, the microbiome of successfully infected sticklebacks varied with parasite genotype. More specifically, we revealed that the association between microbiome and immune gene expression increased in infected individuals and varied with parasite genotype. In addition, we showed that S. solidus hosts a complex endomicrobiome and that bacterial abundance in the parasite correlates with expression of host immune genes. Within this comprehensive analysis we demonstrated that (i) parasites contribute to modulating the host microbiome through both successful and unsuccessful infection, (ii) when infection is successful, the host microbiome varies with parasite genotype due to genotype-dependent variation in parasite immunomodulation, and (iii) the parasite-associated microbiome is distinct from its host and impacts the host immune response to infection.


Assuntos
Cestoides , Infecções por Cestoides , Doenças dos Peixes , Microbiota , Parasitos , Smegmamorpha , Animais , Cestoides/genética , Infecções por Cestoides/genética , Infecções por Cestoides/parasitologia , Doenças dos Peixes/genética , Doenças dos Peixes/parasitologia , Genótipo , Interações Hospedeiro-Parasita/genética , Microbiota/genética , Fenótipo , Smegmamorpha/genética , Smegmamorpha/parasitologia
17.
Biol Open ; 10(11)2021 11 15.
Artigo em Inglês | MEDLINE | ID: mdl-34787304

RESUMO

The protein composition of the cestode Schistocephalus solidus was measured in an experiment simulating the trophic transmission of the parasite from a cold-blooded to a warm-blooded host. The first hour of host colonisation was studied in a model experiment, in which sticklebacks Gasterosteus aculeatus infected with S. solidus were heated at 40°C for 1 h. As a result, a decrease in the content of one tegument protein was detected in the plerocercoids of S. solidus. Sexual maturation of the parasites was initiated in an experiment where S. solidus larvae were taken from fish and cultured in vitro at 40°C for 48 h. Temperature-independent changes in the parasite proteome were investigated by incubating plerocercoids at 22°C for 48 h in culture medium. Analysis of the proteome allowed us to distinguish the temperature-induced genes of S. solidus, as well as to specify the molecular markers of the plerocercoid and adult worms. The main conclusion of the study is that the key enzymes of long-term metabolic changes (glycogen consumption, protein production, etc.) in parasites during colonisation of a warm-blooded host are induced by temperature.


Assuntos
Doenças dos Peixes/parasitologia , Interações Hospedeiro-Parasita , Temperatura Alta/efeitos adversos , Proteoma/metabolismo , Smegmamorpha/parasitologia , Animais , Cestoides
18.
Parasitol Res ; 120(9): 3123-3136, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34410496

RESUMO

The present paper describes Neoechinorhynchus (Neoechinorhynchus) cephali n. sp., an acanthocephalan parasite infecting the intestine of the flathead grey mullet Mugil cephalus from the southwest coast of India. The parasite exhibited a prevalence of 7.40%, mean intensity of 18.5 and abundance of 18-19 worms/infected host. Morphologically, N. (N.) cephali n. sp. is sexually dimorphic, small, cylindrical, slightly curved and creamy white in colour. Females are larger than males, measured 8.87 × 0.88 mm and 5.65 × 0.66 mm, respectively. Proboscis is armed with three circles of six hooks each, which progressively decreases in size posteriorly. Hooks are backwardly curved and robust and tapering with a sharp, pointed tip, striations on the surface and a manubrium at its base. The body is aspinose, trunk surface with micropores and pits and proboscis surface with papilliform structures. The body wall is with five dorsal and two ventral hypodermal nuclei, along with lacunar canals connected by circular anastomoses. Lemnisci are subequal, small lemnisci are uninucleated, and large ones are binucleated. The cement gland is oval, with four giant nuclei; bursa is with many sensory cells. Eggs are elliptical, with concentric shells, and polar prolongation is absent. In the molecular and phylogenetic analyses based on the 18S ribosomal DNA region, the present species stands out with a high bootstrap value and is positioned as a sister branch of N. (N.) dimorphospinus. Based on the differences in morphology, morphometry and molecular and phylogenetic analyses, the present species of acanthocephalan infecting M. cephalus is considered as new, and the name Neoechinorhynchus (Neoechinorhynchus) cephali n. sp. is proposed.


Assuntos
Acantocéfalos , Doenças dos Peixes , Helmintíase Animal , Smegmamorpha , Acantocéfalos/anatomia & histologia , Acantocéfalos/classificação , Animais , Feminino , Doenças dos Peixes/parasitologia , Helmintíase Animal/parasitologia , Índia , Masculino , Filogenia , Smegmamorpha/parasitologia
19.
Parasitol Res ; 120(8): 2769-2778, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34269872

RESUMO

Discoveries of new Hemiclepsis species (Hirudinea: Glossiphoniidae) in East and Southeast Asia were expected. A peculiar freshwater leech was found on the body surface of Asian Swamp Eel Monopterus albus (Synbranchiformes: Synbranchidae) in Jingzhou, Hubei Province, China. Here, we describe this leech species as Hemiclepsis yangtzenensis sp. nov. It could be distinguished from other congeners based on a combination of morphological features such as very small size, translucent body with dense green reticulate markings throughout dorsum, very large posterior sucker with a characteristic reticulate pattern, and two pairs of well-developed drop-like eyespots. It also represents a phylogenetic lineage that is distant from other members of the genus based on the COI and 18S rRNA sequences. This leech species seems to be a specialized fish parasite that could negatively affect farming and wild stocks of Asian Swamp Eel in China.


Assuntos
Sanguessugas , Smegmamorpha , Animais , China , Água Doce , Sanguessugas/anatomia & histologia , Sanguessugas/classificação , Filogenia , Smegmamorpha/parasitologia
20.
Syst Parasitol ; 98(4): 455-476, 2021 08.
Artigo em Inglês | MEDLINE | ID: mdl-34145527

RESUMO

Members of Forticulcitinae Blasco-Costa, Balbuena, Kostadinova & Olson, 2009 include endoparasites of mullet fishes distributed worldwide. Adult specimens were collected from the intestines of white mullet (Mugil curema) and flathead grey mullet (Mugil cephalus) from five localities in the Gulf of Mexico and a single locality in Venezuela. Photogenophores were sequenced for two nuclear molecular markers, the large subunit (LSU) and second internal transcribed spacer (ITS2) of nuclear rDNA. The new sequences were aligned with other sequences downloaded from GenBank. The maximum likelihood and Bayesian inferences were deduced using the combined dataset (LSU + ITS2). The phylogenetic analyses revealed four new lineages belonging to Forticulcitinae. Three new species are described in the present study. Ekuarhuni mexicanus n. sp. can be differentiated from its congeneric species by presenting a longer hermaphroditic sac length (136-180 µm) and a wider testis (91-123 µm). Forticulcita macropharyngis n. sp. and Forticulcita venezuelensis n. sp. are the 8th and 9th species described in Forticulcita. Both species belong to the diminutive morphotype of Forticulcita. Forticulcita macropharyngis n. sp. can be morphologically distinguished from the other congeneric species by the presence of a massive and muscular pharynx (46-110 µm long, 74-106 µm wide). Forticulcita venezuelensis n. sp. is the second species of the studied genus recorded in South America and can be differentiated from congeneric species by possessing the largest testis (138-201 µm long, 83-100 µm wide). Finally, the fourth lineage corresponds to Overstreetoides Andrade-Gómez & García-Varela, 2021; however, few specimens of this lineage were collected, precluding any description of the species. In addition, a key is proposed for differentiating the genera and species of Forticulcitinae.


Assuntos
Filogenia , Smegmamorpha/parasitologia , Trematódeos/classificação , América , Animais , DNA de Helmintos/genética , DNA Ribossômico/genética , Golfo do México , Especificidade da Espécie , Trematódeos/anatomia & histologia , Trematódeos/genética , Venezuela
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