RESUMO
BACKGROUND: Sparganosis is an infection with a larval Diphyllobothriidea tapeworm. From a rare cerebral case presented at a clinic in the UK, DNA was recovered from a biopsy sample and used to determine the causative species as Spirometra erinaceieuropaei through sequencing of the cox1 gene. From the same DNA, we have produced a draft genome, the first of its kind for this species, and used it to perform a comparative genomics analysis and to investigate known and potential tapeworm drug targets in this tapeworm. RESULTS: The 1.26 Gb draft genome of S. erinaceieuropaei is currently the largest reported for any flatworm. Through investigation of ß-tubulin genes, we predict that S. erinaceieuropaei larvae are insensitive to the tapeworm drug albendazole. We find that many putative tapeworm drug targets are also present in S. erinaceieuropaei, allowing possible cross application of new drugs. In comparison to other sequenced tapeworm species we observe expansion of protease classes, and of Kuntiz-type protease inhibitors. Expanded gene families in this tapeworm also include those that are involved in processes that add post-translational diversity to the protein landscape, intracellular transport, transcriptional regulation and detoxification. CONCLUSIONS: The S. erinaceieuropaei genome begins to give us insight into an order of tapeworms previously uncharacterized at the genome-wide level. From a single clinical case we have begun to sketch a picture of the characteristics of these organisms. Finally, our work represents a significant technological achievement as we present a draft genome sequence of a rare tapeworm, and from a small amount of starting material.
Assuntos
Diphyllobothrium/genética , Genoma , Esparganose/genética , Spirometra/genética , Animais , Sequência de Bases , Biópsia , Encéfalo/parasitologia , Encéfalo/patologia , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Esparganose/parasitologia , Spirometra/parasitologia , Reino UnidoRESUMO
OBJECTIVE: To predict structure and function of translationally controlled tumor protein (TCTP) from Spirometra mansoni by bioinformatics technology, and to provide a theoretical basis for further study. METHODS: Open reading frame (ORF) of EST sequence from Spirometra mansoni was obtained by ORF finder and was translated into amino acid residue by DNAclub. The structure domain was analyzed by Blast. By the method of online analysis tools: Protparam, InterProScan, protscale, SignalP-3.0, PSORT II, BepiPred, TMHMM, VectorNTI Suite 9 packages and Phyre2, the structure and function of the protein were predicted and analyzed. RESULTS: The results showed that the EST sequence was Sm TCTP with 173 amino acid residues, theoretical molecular weight was 19 872.0 Da. The protein has the closest evolutionary status with Clonorchis sinensis, Schistosoma mansoni, and Schistosoma japonicum. Then it had no signal peptide site and transmembrane domain. Secondary structure of TCTP contained two α -helices and eight ß -strands. CONCLUSIONS: Sm TCTP was a variety of biological functions of protein that may be used as a vaccine candidate molecule and drug target.
Assuntos
Biomarcadores Tumorais/química , Infecções por Cestoides/veterinária , Proteínas de Helminto/química , Spirometra/genética , Spirometra/parasitologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Biomarcadores Tumorais/genética , Biomarcadores Tumorais/metabolismo , Infecções por Cestoides/parasitologia , Biologia Computacional , Doenças do Cão , Cães , Etiquetas de Sequências Expressas , Proteínas de Helminto/genética , Proteínas de Helminto/metabolismo , Helmintos/química , Helmintos/classificação , Helmintos/genética , Fases de Leitura Aberta , Filogenia , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Spirometra/química , Spirometra/metabolismo , Proteína Tumoral 1 Controlada por TraduçãoRESUMO
Se describe un céstodo encontrado en el intestino delgado de gatos provenientes de dos zonas diferentes de Costa Rica (Liberia-Guanacaste y Atenas-Alajuela). De acuerdo con un análisis comparativo morfológico se concluye que los ejemplares hallados corresponden a parásitos del género Spirometra ya que presentan el útero espiralado y huevecillos con los extremos puntiagudos, a diferencia del útero en roseta y huevecillos con extremos redondeados que se encuentran en el género Diphyllobothrium. Dado que presentan un número variable de vueltas en la parte anterior del útero y la vagina es ondulada se clasifican como Spirometra mansoni, siendo éste el primer reporte de este parásito en el país. Se hace una corta revisión de la importancia de este parasito para el ser humano.
