Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 4 de 4
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Pol J Vet Sci ; 19(1): 7-14, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27096782

RESUMO

The aim of this study was to characterize Staphylococcus pseudintermedius for its antimicrobial resistance and virulence factors with a special focus on methicillin-resistant (MRSP) strains isolated from sick dogs in Lithuania. Clinically sick adult dogs suffering from infections (n=214) and bitches with reproductive disorders (n=36) from kennels were selected for the study. Samples (n=192) from the 250 tested (76.8%) dogs were positive for Staphylococcus spp. Molecular profiling using the species-specific nuc gene identified 51 isolates as S. pseudintermedius (26.6% from a total number of isolated staphylococci) of which 15 isolates were identified as MRSP. Ten MRSP isolates were isolated from bitches with reproductive disorders from two large breeding kennels. Data on susceptibility of S. pseudintermedius to different antimicrobials revealed that all isolates were susceptible to vancomycin, daptomycin and linezolid. Two isolates (3.9%) were resistant to rifampicin. A high resistance was seen towards penicillin G (94.1%), tetracycline (64.7%) and macrolides (68.7%). Resistance to fluoroquinolones ranged from 25.5% (gatifloxacin) to 31.4% (ciprofloxacin). The most prevalent genes encoding resistance included blaZ, aac(6')-Ie-aph(2'')-Ia, mecA, and tet(M). The Luk-I gene encoding a leukotoxin was detected in 29% of the isolates, whereas the siet gene encoding exfoliative toxin was detected in 69% of the S. pseudintermedius isolates. This report of MRSP in companion animals represents a major challenge for veterinarians in terms of antibiotic therapy and is a concern for both animal and public health.


Assuntos
Antibacterianos/farmacologia , Doenças do Cão/microbiologia , Infecções Cutâneas Estafilocócicas/veterinária , Staphylococcus intermedius/efeitos dos fármacos , Animais , Doenças do Cão/epidemiologia , Cães , Farmacorresistência Bacteriana Múltipla , Regulação Bacteriana da Expressão Gênica/fisiologia , Lituânia/epidemiologia , Infecções Cutâneas Estafilocócicas/epidemiologia , Infecções Cutâneas Estafilocócicas/microbiologia , Staphylococcus intermedius/genética , Staphylococcus intermedius/metabolismo , Fatores de Virulência/genética , Fatores de Virulência/metabolismo
2.
J Clin Microbiol ; 54(3): 745-8, 2016 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-26677248

RESUMO

The performance of a rapid penicillin-binding protein 2a (PBP2a) detection assay, the Alere PBP2a culture colony test, was evaluated for identification of PBP2a-mediated beta-lactam resistance in human and animal clinical isolates of Staphylococcus intermedius group, Staphylococcus lugdunensis, and Staphylococcus schleiferi. The assay was sensitive and specific, with all PBP2a-negative and PBP2a-positive strains testing negative and positive, respectively.


Assuntos
Cromatografia de Afinidade , Proteínas de Ligação às Penicilinas/metabolismo , Peptídeo Sintases/metabolismo , Staphylococcus intermedius/metabolismo , Staphylococcus lugdunensis/metabolismo , Animais , Cromatografia de Afinidade/métodos , Cromatografia de Afinidade/normas , Humanos , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Infecções Estafilocócicas/microbiologia , Staphylococcus intermedius/isolamento & purificação , Staphylococcus lugdunensis/isolamento & purificação
3.
Mol Gen Mikrobiol Virusol ; 34(3): 83-89, 2016.
Artigo em Russo | MEDLINE | ID: mdl-30383929

RESUMO

The changes in the nomenclature of species in the genus Staphylococcus, including the most pathogenic cluster of the coagulase-positive staphylococci, are represented. Presently, besides S. aureus, this cluster consists of 6 species: S. intermedius, S. schleiferi ssp. coagulans, S. lutrae, S. hyicus, S. pseudintermedius, and S. delphini. A particular attention was paid to the Staphylococcus intermedius group (SIG), which includes three closely related coagulase-positive bacterial species: S. intermedius, S. pseudintermedius, and S. delphini. The hosts of SIG species are various mammals and birds, which live in a close contact with humans. The current knowledge about the virulence factors and pathogenicity for animals and humans are analyzed. The diffic6lties of the species identification, the features of ecology and epidemiology, antimicrobial resistance were reviewed. The biological features of S. pseudintermedius, which has the greatest similarity with S. aureus, are considered in the context of the properties of newly emerging pathogens.


Assuntos
Farmacorresistência Bacteriana , Infecções Estafilocócicas , Staphylococcus intermedius , Fatores de Virulência/metabolismo , Animais , Humanos , Infecções Estafilocócicas/tratamento farmacológico , Infecções Estafilocócicas/epidemiologia , Staphylococcus intermedius/metabolismo , Staphylococcus intermedius/patogenicidade
4.
Peptides ; 68: 190-6, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25784287

RESUMO

Royalisin is a 5.5-kDa antibacterial peptide isolated from the royal jelly of the honeybee (Apis mellifera). The antimicrobial activity of royalisin against fungi, Gram-positive and Gram-negative bacteria has been revealed. Compared with another insect antibacterial peptide, there is an extra stretch of 11 amino acid residues at the C-terminus of royalisin. In this study, a recombinant shortened form of royalisin named as royalisin-D, was constructed without the 11 amino acid residues at the C-terminal of royalisin and linked to the C-terminal of oleosin by an inteinS fragment. The recombinant protein was overexpressed in Escherichia coli, purified by artificial oil body system and subsequently released through self-splicing of inteinS induced by the changes of temperature. The antibacterial activity of royalisin-D was compared with royalisin via minimal inhibitory concentration (MIC) assay, minimal bactericidal concentration (MBC) assay, microbial adhesion to solvents (MATS) methods, and cell membrane permeability. Furthermore, the recombinant royalisin and royalisin-D have also been treated with the reducing agent of disulfide bonds, dithiothreitol (DTT), to investigate the importance of the intra-disulfide bond in royalisin. In our results, royalisin-D exhibited similar antimicrobial activity to royalisin. Royalisin and royalisin D lost their antimicrobial activities when the intra-disulfide bonds were reduced by DDT. The intra-disulfide bond plays a more important role than the extra stretch of 11 amino acid residues at the C-terminus of royalisin in terms of the antimicrobial properties of the native royalisin.


Assuntos
Antibacterianos/farmacologia , Proteínas de Insetos/farmacologia , Proteínas/farmacologia , Animais , Antibacterianos/química , Abelhas , Ácidos Graxos/química , Interações Hidrofóbicas e Hidrofílicas , Proteínas de Insetos/química , Peptídeos e Proteínas de Sinalização Intercelular , Testes de Sensibilidade Microbiana , Estrutura Molecular , Permeabilidade , Proteínas/química , Pseudomonas aeruginosa/efeitos dos fármacos , Pseudomonas aeruginosa/metabolismo , Staphylococcus intermedius/efeitos dos fármacos , Staphylococcus intermedius/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...