Effects of ethanamizuril, sulfachlorpyridazine or their combination on cecum microbial community and metabolomics in chickens infected with Eimeria tenella.

Coccidiostat and antibiotics are widely used in poultry industry, but their effects on cecum microbial community and metabolomics in chickens infected with coccidia have been rarely studied. In this study, we analyzed the changes of microbiota and metabolomic which associated with Eimeria tenella infection in 8 days of age chickens in the presence or absence of ethanamizuril, sulfachlorpyridazine or their combinations treatment for 3 consecutive days. 16S rRNA gene sequencing and LC-MS/MS analyses were used to profile the cecal microbiome and metabolome in each group of chickens on 7 days post-infection. The results showed that coccidial infection induced significant perturbations in the distribution of microbial taxonomy and the metabolism of physiological functional molecules in cecal contents. Ethanamizuril treatment seemed to transform microbiota into a steady state conducive to animal health, and sulfachlorpyridazine treatment alleviated the growth of potentially harmful bacteria such as Escherichia-Shigella. The change trends of metabolites such as n-carbamoylglutamic acid were consistent with the anticoccidial effect of ethanamizuril. The combinations of ethanamizuril and sulfachlorpyridazine at low-dose had little effect on gut microbiota, metabolism and anticoccidial effect. These data indicate that the cecal microbiota and metabolic status of chickens infected with E. tenella following ethanamizuril treatment could be used to monitor the response to drug efficacy. This study provides a new system approach to elucidate the microbiota, metabolic and therapeutic effects of the combination of coccidiostat and antibiotics in the context of avian coccidiosis.

Diagnosis and Management of Diabetes Mellitus in a Bali Mynah (Leucopsar rothschildi).

An 18-year-old female Bali mynah (Leucopsar rothschildi) was presented for polyphagia, weight loss, and incoordination. Diabetes mellitus was diagnosed based on the history and clinical findings, including persistent hyperglycemia with concurrent hypoinsulinemia and glucosuria. A treatment protocol was developed that led to improvement of clinical signs and management of hyperglycemia over several months. Because of the advanced age of the animal, difficulty in maintaining euglycemia, and the stress of handling and treatment, euthanasia was elected 167 days after initial presentation. At postmortem examination, no pancreatic lesions were detected histologically that would account for the diabetes mellitus. To our knowledge this is the first reported case of diabetes mellitus and clinical management of this condition in a passerine species.

Pharmacokinetics and therapeutic potential for repeated oral doses of trimethoprim/sulphachlorpyridazine in horses.

The pharmacokinetic parameters of a powder formulation of trimethoprim/sulphachlorpyridazine were studied in eight healthy horses which received 5 mg/kg trimethoprim and 25 mg/kg sulphachlorpyridazine 12-hourly with concentrate for five days. The intake of the medicated concentrate by the horses was variable during the first two days, but after they became accustomed to the taste the intake by all the horses during the last three days was good. Faecal samples taken before and on the last day of the drug administrations were negative when cultured for salmonella. Compared with the results of a previous single-dose experiment, higher plasma concentrations and a higher area under the curve for both the drugs were observed. The repeated doses provided plasma concentrations above the minimal inhibitory concentration for Streptococcus zooepidemicus, S equi, Actinobacillus equuli and Rhodococcus equi isolated from the respiratory tract of horses. Synergism between the two drugs occurred at different drug concentration ratios with different bacterial species.

Some pharmacokinetic aspects of four sulphonamides and trimethoprim, and their therapeutic efficacy in experimental Escherichia coli infection in poultry.

Pharmacokinetic studies in broilers and layers of different sulphonamides indicate a good absorption and a long elimination half-life (of sulphaquinoxaline, sulphadimidine and to a lesser degree sulphadiazine) resulting in high plasma concentrations during drinking water medication in the recommended therapeutic doses. In contrast drinking water medication with high concentrations of trimethoprim (up to 1,320 mg/liter) resulted in a maximal mean plasma concentration of 1.2 micrograms/ml. Very good therapeutic effects were demonstrated in broilers experimentally infected with a sulphonamide-susceptible E. coli strain when treated with sulphaquinoxaline (200 mg/liter), sulphadimidine sodium (2 gram/liter), sulphachloropyridazine 30 per cent (1 gram/liter) and to a lesser degree sulphadiazine sodium (250 mg/liter). Synergism was demonstrated between trimethoprim and sulphadiazine (1:5). The combination of trimethoprim with sulphaquinoxaline (1:3) did not induce better therapeutic effects than sulphaquinoxaline in proportional doses. However, significant synergism was demonstrated between trimethoprim and both sulphonamides in treatment of experimental infection with sulphonamide-resistant E. coli. No signs resembling sulphonamide intoxication were observed during these studies.

[Action of sodium sulfachlorpyrazine (ESB3) on the endogenous development of Eimeria tenella in the experimental infestation of chickens]. / Prouchvane deistvieto na sulfakhlorpirazin-natriia (ESB3) vurkhu endogennoto razvitie na Eimeria tenella pri eksperimentalna invaziia na pileta.

Studies were carried out on the action of sulfachlorpyrisine-Na (ESB3) on the endogenic development of Eimeria tenella in the case of experimental infection in birds. Most sensitive to the preparation were the endogenic forms between the 48 h and the 72 nd following the act of infection. Given to birds on the day of infection and 24 hours later it produced no harmful effect on the further endogenic development of E. tenella. When the preparation was applied at the 72 nd hour it led to the degeneration of most of the II generation schizonts and inhibited their further development. As a result no oocysts were found in the feces of birds up to the 10 th-11 th day after infection. When applied at the 92 nd and the 120 th hour sulfachlorpyrisine-Na interfered with the development of the already finite forms of gametogony without concurrent degenerative effects.

[Chemoprophylaxis and therapy of coccidiosis in the rock partridge, Alectoris graeca cypriatis]. / Khimioprofilaktika i terapiia na koktsidiozata po keklitsite Alektoris graeca cypriatis.

A number of coccidiostatica--amprolium (Merek Sharp & Dohme), both for the prevention and treatment, DOT Solubile (By-Gulden) and Esb3 (Ciba Geigy) for treatment--were tested with artificially raised rock partridges on an infected farm. The trials were carried out with a total of 12300 young (12-day-old) birds suffering from spontaneous outbreaks of coccidiosis and 60 such birds with experimental coccidiosis. The comparative study on the results obtained revealed that amprolium produced a good prophylactic effect and a low therapeutic one in cases of clinical coccidioses, used at the rate of 125 mg/kg and offered with the forage mixture. DOT at 30 g/10 1 of water had a pronounced therapeutic effect in the course of 5 days. The preparation Esb3 at the rate of 1 g per one liter of water for 3 days was tested in experimentally induced coccidiosis (E. kofoidi). It produced good therapeutic effects with the highest coccidiosis index ever noted on the second day following infection. It can be applied on occasions of E. kofoidi-induced coccidiosis.