RESUMO
In order to develop an efficient large-scale extraction of secologanin from Symphoricarpos albus, different methods have been compared. Ultrasonication with organic solvents and water, microwave-assisted extraction and hot water extraction methods were evaluated for their efficiencies. Among the methods tested, ultrasonication with methanol showed the highest yield of secologanin (3.35 +/- 0.24 mg/g fresh weight). For reliable quantification of secologanin a 1H-NMR method was developed. The experiment was performed by the analysis of the integral of the signal of H-9, which was well separated in the range delta 7.4-7.5 in the 1H-NMR spectrum. The quantity of the compound was calculated from the relative ratio of intensity of the target peak to the known amount of internal standard, 200 microg of gallic acid. This method allows rapid and simple quantification of secologanin in 5 min without any pre-purification steps.
Assuntos
Iridoides/isolamento & purificação , Symphoricarpos/química , Técnicas de Química Analítica/métodos , Frutas/química , Glucosídeos Iridoides , Iridoides/química , Micro-Ondas , Ressonância Magnética Nuclear Biomolecular/métodos , SonicaçãoRESUMO
The content of phenolic acids was determined in the extracts and fractions from leaves, flowers and fruits of Symphoricarpos albus (L.) Blake (Caprifoliaceae) by the Arnov's method. Antimicrobial activity of all extracts against Gram-positive anti Gram-negative microorganisms has been tested.
Assuntos
Anti-Infecciosos/farmacologia , Hidroxibenzoatos/análise , Symphoricarpos/química , Bactérias/efeitos dos fármacos , Fungos/efeitos dos fármacos , Indicadores e Reagentes , Testes de Sensibilidade Microbiana , Extratos Vegetais/químicaRESUMO
The leaves, flowers and fruits of Symphoricarpos albus (L.) Blake (Caprifoliaceae) were analysed for the presence of phenolic acids. Eleven free and liberated by hydrolysis phenolic acids were identified by TLC, HPLC and spectral (UV) methods. Moreover, the HPLC method was applied for the quantitative determination of phenolic acids in the analysed fractions.
