Recent Advances in Electrochemical Biosensors for the Detection of Salmonellosis: Current Prospective and Challenges.

Salmonellosis is a major cause of foodborne infections, caused by Salmonella, posing a major health risk. It possesses the ability to infiltrate the food supply chain at any point throughout the manufacturing, distribution, processing or quality control process. Salmonella infection has increased severely and requires effective and efficient methods for early monitoring and detection. Traditional methods, such as real-time polymerase chain reaction and culture plate, consume a lot of time and are labor-intensive. Therefore, new quick detection methods for on-field applications are urgently needed. Biosensors provide consumer-friendly approaches for quick on-field diagnoses. In the last few years, there has been a surge in research into the creation of reliable and advanced electrochemical sensors for the detection of Salmonella strains in food samples. Electrochemical sensors provide extensive accuracy and reproducible results. Herein, we present a comprehensive overview of electrochemical sensors for the detection of Salmonella by focusing on various mechanisms of electrochemical transducer. Further, we explain new-generation biosensors (microfluidics, CRISPR- and IOT-based) for point-of care applications. This review also highlights the limitations of developing biosensors in Salmonella detection and future possibilities.

Recent trends in application of nanomaterials for the development of electrochemical microRNA biosensors.

The biology of the late twentieth century was marked by the discovery in 1993 of a new class of small non-coding ribonucleic acids (RNAs) which play major roles in regulating the translation and degradation of messenger RNAs. These small RNAs (18-25 nucleotides), called microRNAs (miRNAs), are implied in several biological processes such as differentiation, metabolic homeostasis, or cellular apoptosis and proliferation. The discovery in 2008 that the presence of miRNAs in body fluids could be correlated with cancer (prostate, breast, colon, lung, etc.) or other diseases (diabetes, heart diseases, etc.) has made them new key players as biomarkers. Therefore, miRNA detection is of considerable significance in both disease diagnosis and in the study of miRNA function. Until these days, more than 1200 miRNAs have been identified. However, traditional methods developed for conventional DNA does not apply satisfactorily for miRNA, in particular due to the low expression level of these miRNA in biofluids, and because they are very short strands. Electrochemical biosensors can provide this sensitivity and also offer the advantages of mass fabrication, low-cost, and potential decentralized analysis, which has wide application for microRNAs sensing, with many promising results already reported. The present review summarizes some newly developed electrochemical miRNA detection methods.

Application of Ionic Liquids in Electrochemistry-Recent Advances.

In this review, the roles of room temperature ionic liquids (RTILs) and RTIL based solvent systems as proposed alternatives for conventional organic electrolyte solutions are described. Ionic liquids are introduced as well as the relevant properties for their use in electrochemistry (reduction of ohmic losses), such as diffusive molecular motion and ionic conductivity. We have restricted ourselves to provide a survey on the latest, most representative developments and progress made in the use of ionic liquids as electrolytes, in particular achieved by the cyclic voltammetry technique. Thus, the present review comprises literature from 2015 onward covering the different aspects of RTILs, from the knowledge of these media to the use of their properties for electrochemical processes. Out of the scope of this review are heat transfer applications, medical or biological applications, and multiphasic reactions.

Waste or Gold? Bioelectrochemical Resource Recovery in Source-Separated Urine.

In recent years, source-separated human urine has been highlighted as an effective resource for energy and nutrient recovery. However, even though several technologies exist for resource recovery, they have not been widely implemented. Among these technologies, bioelectrochemical systems (BESs) hold promise as technically and economically interesting alternatives for sustainable resource recovery from source-separated urine. Here, we review the resource recovery performance of BESs, including microbial fuel cells (MFCs) and microbial electrolysis cells (MECs), fed with source-separated urine over the past decade, and suggest an effective path forward toward their widespread implementation.

Impedimetric Aptamer-Based Biosensors: Principles and Techniques.

Aptamers are a specific class of ligands with high affinities comparable to antibodies, which are selected and synthesized in vitro. In combination with impedance spectroscopy as sensitive measurement method, we gain a class of biosensors with high potential for handheld devices and point-of-care tests. In this review, we report on recent advances in aptamer-based impedimetric biosensors. Besides giving a short summary of electrochemical measurement techniques, the most exciting innovative developments of detection strategies in the last decades are reviewed. Finally, important criteria for the comparison of aptamer-based biosensors are discussed.

Cytokine and Cancer Biomarkers Detection: The Dawn of Electrochemical Paper-Based Biosensor.

Although the established ELISA-based sensing platforms have many benefits, the importance of cytokine and cancer biomarkers detection for point-of-care diagnostics has propelled the search for more specific, sensitive, simple, accessible, yet economical sensor. Paper-based biosensor holds promise for future in-situ applications and can provide rapid analysis and data without the need to conduct in a laboratory. Electrochemical detection plays a vital role in interpreting results obtained from qualitative assessment to quantitative determination. In this review, various factors affecting the design of an electrochemical paper-based biosensor are highlighted and discussed in depth. Different detection methods, along with the latest development in utilizing them in cytokine and cancer biomarkers detection, are reviewed. Lastly, the fabrication of portable electrochemical paper-based biosensor is ideal in deliberating positive societal implications in developing countries with limited resources and accessibility to healthcare services.

Recent Advances in Ochratoxin A Electrochemical Biosensors: Recognition Elements, Sensitization Technologies, and Their Applications.

Ochratoxin A (OTA) is a class of mycotoxin that are mainly produced by Aspergillus and Penicillium and widely found in plant origin food. OTA-contaminated foods can cause serious harm to animals and humans, while high stability of OTA makes it difficult to remove in conventional food processing. Thus, sensitive and rapid detection of OTA undoubtedly plays an important role in OTA prevention and control. In this paper, the conventional and novel methods of OTA at home and abroad are summarized and compared. The latest research progress and related applications of novel OTA electrochemical biosensors are mainly described with a new perspective. We innovatively divided the recognition element into single and combined recognition elements. Specifically, signal amplification technologies applied to the OTA electrochemical aptasensor are proposed. Furthermore, summary of the current limitations and future challenges in OTA analysis is included, which provide reference for the further research and applications.

Pesticides determination in foods and natural waters using solid amalgam-based electrodes: Challenges and trends.

The intensive use of pesticides in agricultural practices has promoted the appearance of environmental and public health problems. So, there has been a sharp increase in the development of simple, fast, sensitive, selective, and low-cost methods to analyse pesticides. Among them, electroanalytical methods have been frequently employed; however, the performance of these methods is strongly influenced by the working electrode material and so an adequate choice is critical to success of the analysis. Solid amalgam-based electrodes have been widely used; this review critically discusses the evolution of the preparation and use of these electrodes and their application in analysis of pesticides from different chemical classes, indicating challenges and trends in pesticide electroanalysis. The relationship between pesticides' chemical structures and electrochemical behaviour on a mercury-based electrode is explored in order to indicate the use of electroanalysis in pesticide determination.

Recent Advances in Electrochemical and Optical Sensing of Dopamine.

Nowadays, several neurological disorders and neurocrine tumours are associated with dopamine (DA) concentrations in various biological fluids. Highly accurate and ultrasensitive detection of DA levels in different biological samples in real-time can change and improve the quality of a patient's life in addition to reducing the treatment cost. Therefore, the design and development of diagnostic tool for in vivo and in vitro monitoring of DA is of considerable clinical and pharmacological importance. In recent decades, a large number of techniques have been established for DA detection, including chromatography coupled to mass spectrometry, spectroscopic approaches, and electrochemical (EC) methods. These methods are effective, but most of them still have some drawbacks such as consuming time, effort, and money. Added to that, sometimes they need complex procedures to obtain good sensitivity and suffer from low selectivity due to interference from other biological species such as uric acid (UA) and ascorbic acid (AA). Advanced materials can offer remarkable opportunities to overcome drawbacks in conventional DA sensors. This review aims to explain challenges related to DA detection using different techniques, and to summarize and highlight recent advancements in materials used and approaches applied for several sensor surface modification for the monitoring of DA. Also, it focuses on the analytical features of the EC and optical-based sensing techniques available.

Bioelectrofuel Synthesis by Nanoenzymes: Novel Alternatives to Conventional Enzymes.

Recent bioinspired efforts of designing novel nanoenzyme-based electrocatalysts are driven by the urgency of making bioelectrofuels more affordable and efficient. Unlike natural enzymes, nanoenzyme-modified electrodes with large surface areas enclose numerous biomimicking active sites to facilitate enhanced microbial growth followed by increased reactant-to-bioelectrofuel conversion.

Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review.

Neurotransmitters are endogenous chemical messengers which play an important role in many of the brain functions, abnormal levels being correlated with physical, psychotic and neurodegenerative diseases such as Alzheimer's, Parkinson's, and Huntington's disease. Therefore, their sensitive and robust detection is of great clinical significance. Electrochemical methods have been intensively used in the last decades for neurotransmitter detection, outclassing more complicated analytical techniques such as conventional spectrophotometry, chromatography, fluorescence, flow injection, and capillary electrophoresis. In this manuscript, the most successful and promising electrochemical enzyme-free and enzymatic sensors for neurotransmitter detection are reviewed. Focusing on the activity of worldwide researchers mainly during the last ten years (2010-2019), without pretending to be exhaustive, we present an overview of the progress made in sensing strategies during this time. Particular emphasis is placed on nanostructured-based sensors, which show a substantial improvement of the analytical performances. This review also examines the progress made in biosensors for neurotransmitter measurements in vitro, in vivo and ex vivo.

Dengue virus: a review on advances in detection and trends - from conventional methods to novel biosensors.

Dengue virus is an important arbovirus infection which transmitted by the Aedes female mosquitoes. The attempt to control and early detection of this infection is a global public health issue at present. Because of the clinical importance of its detection, the main focus of this review is on all of the methods that can offer the new diagnosis strategies. The advantages and disadvantages of reported methods have been discussed comprehensively from different aspects like biomarkers type, sensitivity, accuracy, rate of detection, possibility of commercialization, availability, limit of detection, linear range, simplicity, mechanism of detection, and ability of usage for clinical applications. The optical, electrochemical, microfluidic, enzyme linked immunosorbent assay (ELISA), and smartphone-based biosensors are the main approaches which developed for detection of different biomarkers and serotypes of Dengue virus. Future efforts in miniaturization of these methods open the horizons for development of commercial biosensors for early-diagnosis of Dengue virus infection. Graphical abstract Transmission of Dengue virus by the biting of an Aedes aegypti mosquito, the symptoms of Dengue hemorrhagic fever and the structure of Dengue virus and application of biosensors for its detection.

Dissecting the Catecholamines: How New Approaches Will Facilitate the Distinction between Noradrenergic and Dopaminergic Systems.

To overcome the limitations of fast scan cyclic voltammetry (FSCV) to discriminate between catecholamines, we discuss new approaches to monitor the dynamics of these neurochemicals with high spatial, genetic, and temporal specificity.

Recent electrochemical methods in electrochemical degradation of halogenated organics: a review.

Halogenated organics are widely used in modern industry, agriculture, and medicine, and their large-scale emissions have led to soil and water pollution. Electrochemical methods are attractive and promising techniques for wastewater treatment and have been developed for degradation of halogenated organic pollutants under mild conditions. Electrochemical techniques are classified according to main reaction pathways: (i) electrochemical reduction, in which cleavage of C-X (X = F, Cl, Br, I) bonds to release halide ions and produce non-halogenated and non-toxic organics and (ii) electrochemical oxidation, in which halogenated organics are degraded by electrogenerated oxidants. The electrode material is crucial to the degradation efficiency of an electrochemical process. Much research has therefore been devoted to developing appropriate electrode materials for practical applications. This paper reviews recent developments in electrode materials for electrochemical degradation of halogenated organics. And at the end of this paper, the characteristics of new combination methods, such as photocatalysis, nanofiltration, and the use of biochemical method, are discussed.

Functional Sensing Interfaces of PEDOT:PSS Organic Electrochemical Transistors for Chemical and Biological Sensors: A Mini Review.

Organic electrochemical transistors (OECTs) are promising devices for applications in in vitro and in vivo measurements. OECTs have two important sensing interfaces for signal monitoring: One is the gate electrode surface; the other is the channel surface. This mini review introduced the new developments in chemical and biological detection of the two sensing interfaces. Specific focus was given on the modification technological approaches of the gate or channel surface. In particular, some unique strategies and surface designs aiming to facilitate signal-transduction and amplification were discussed. Several perspectives and current challenges of OECTs development were also briefly summarized.

Nanomaterial based electrochemical sensing of the biomarker serotonin: a comprehensive review.

This review (with 131 references) summarizes the progress made in the past years in the field of nanomaterial based sensing of serotonin (5-HT). An introduction summarizes the significant role of 5-HT as a biomarker for several major diseases, methods for its determination and the various kinds of nanomaterials for use in electrochemical sensing process relies principally on a precise choice of electrodes. The next main section covers nanomaterial based methods for sensing 5-HT, with subsections on electrodes modified with carbon nanotubes, graphene related materials, gold nanomaterials, and by other nanomaterials. A concluding section discusses future perspectives and current challenges of 5-HT determination. Graphical abstract Conceptual design of electrochemical sensing process of the biomarker serotonin by using nanomaterials and the role of 5-HTas biomarker in the body from preclinical to clincal.

Current state of green reduction strategies: Solution-processed reduced graphene oxide for healthcare biodetection.

Reduction of graphene oxide becomes an alternative way to produce a scalable graphene and the resulting nanomaterial namely reduced graphene oxide (rGO) has been utilized in a wide range of potential applications. In this article, the level of green reduction strategies, especially the solution-based reduction methods are overviewed based on recent progression, to get insights towards biomedical applications. The degrees of gaining tips with the solution-based green reduction methods, conditions, complexity and the resulting rGO characteristics have been elucidated comparatively. Moreover, the application of greenly produced rGO in electrochemical biosensors has been elucidated as well as their electrical performance in term of linear range and limit of detections for various healthcare biological analytes. In addition, the characterization scheme for graphene-based materials and the analyses on the reduction especially for the solution-based green reduction methods are outlined for the future endeavours.

Electrochemical Reduction of Carbon Dioxide to Value-Added Products: The Electrocatalyst and Microbial Electrosynthesis.

The electrochemical reduction of carbon dioxide (CO2 ) to value-added products obtains great attention and investigation worldwide in recent years. The commercialization of this green process relies on the progress of relating high-performance electrocatalysts and their feasibility with proper reactor design. The microbial electrosynthesis (MES) is an alternative route to reduce CO2 with electroactive bio-film electrode as catalyst. This review presents the research status and development of cathode catalysts, particularly focusing on the active sites and development tendency, for highly efficient electrochemical reduction CO2 from personal viewpoint. Some of our results are also presented to exhibit contributions. MES shows a similar process to the typical electrochemical reduction of CO2 . Their combination is an important trend, and the future research in this field is full of challenges and opportunities.

Molecularly Imprinted Polymers in Electrochemical and Optical Sensors.

Molecular imprinting is the process of template-induced formation of specific recognition sites in a polymer. Synthetic receptors prepared using molecular imprinting possess a unique combination of properties such as robustness, high affinity, specificity, and low-cost production, which makes them attractive alternatives to natural receptors. Improvements in polymer science and nanotechnology have contributed to enhanced performance of molecularly imprinted polymer (MIP) sensors. Encouragingly, recent years have seen an increase in high-quality publications describing MIP sensors for the determination of biomolecules, drugs of abuse, and explosives, driving toward applications of this technology in medical and forensic diagnostics. This review aims to provide a focused overview of the latest achievements made in MIP-based sensor technology, with emphasis on research toward real-life applications.