1.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub
; 149(2): 261-4, 2005 Dec.
Artigo
em Inglês
| MEDLINE
| ID: mdl-16601766
RESUMO
The aim of this work was isolation and purification of the major immunodominant protein, Outer surface protein C (OspC) of three members of the species group Borrelia burgdorferi, the causative agent of Lyme disease. Our aim was to obtain this protein in a quantity and purity sufficient for immunization of experimental animals. For optimalization of protein purification's yield we used immobilized metal ion affinity chromatography (IMAC) under different conditions. The greatest efficiency was achieved by using of HiTrap Chelating Column under native conditions.