RESUMO
Crotalus durissus terrificus venom (CDT venom) was irradiated with four different doses of gamma rays (2, 3, 5 and 10 kGy) from a 60Co source and their structural, toxic and immunogenic properties were analysed. Venom irradiated with 2 and 3 kGy were, respectively, 2.7 and 13.5 times less toxic than the native one, whereas the 5 or 10 kGy irradiated venom were at least 100 times less toxic than nonirradiated venom. Irradiated venom with all doses were immunogenic and the antibodies elicited by them were able to recognise the native venom in ELISA. However the toxoid produced with 2 kGy irradiation dose had its immunogenicity improved. Antisera raised against this toxoid had a higher neutralising capacity than those produced against the native venom. Irradiation of venom with 2 kGy dose was the most effective to inactivate the CDT venom toxicity and improve its immunogenicity.
Assuntos
Crotalus/fisiologia , Raios gama , Soros Imunes/efeitos da radiação , Imunização Passiva/métodos , Venenos de Serpentes/efeitos da radiação , Venenos de Serpentes/toxicidade , Animais , Western Blotting , Cromatografia em Gel , Relação Dose-Resposta a Droga , Eletroforese em Gel de Poliacrilamida , Ensaio de Imunoadsorção Enzimática , Feminino , Testes de Neutralização , Coelhos , Venenos de Serpentes/imunologia , Taxa de SobrevidaRESUMO
The effect of irradiation of the aqueous solution of L. m. muta venom was evaluated with thermic neutrons from Uranium-235 employing doses of 1.61 to 5.19 Gray. The venom was examined for protein content by the Folin Lowry Method modified by Stauffer; for acute toxicity by intraperitoneal route estimation in mice and for immunochemical tests by the antigen-antibody reactions evaluation. Neutronic radiation affects all evaluated parameters in venom (decrease in protein content levels, increase of LD50 values and decrease in the number of precipitating antigen-antibody) as shown by immunodiffusion and immunoelectrophoresis
Assuntos
Animais , Camundongos , Venenos de Serpentes/efeitos da radiação , Urânio , Viperidae , Doses de Radiação , Imunodifusão , Imunoeletroforese , Dose Letal Mediana , Proteínas/efeitos da radiação , Proteínas/química , Venenos de Serpentes/químicaRESUMO
The effect of irradiation of the aqueous solution of L. m. muta venom was evaluated with thermic neutrons from Uranium-235 employing doses of 1.61 to 5.19 Gray. The venom was examined for protein content by the Folin Lowry Method modified by Stauffer; for acute toxicity by intraperitoneal route estimation in mice and for immunochemical tests by the antigen-antibody reactions evaluation. Neutronic radiation affects all evaluated parameters in venom (decrease in protein content levels, increase of LD50 values and decrease in the number of precipitating antigen-antibody) as shown by immunodiffusion and immunoelectrophoresis.