Assuntos
Bactérias/genética , Bactérias/patogenicidade , Genoma Bacteriano , Genômica , Bordetella bronchiseptica/genética , Bordetella bronchiseptica/patogenicidade , Bordetella parapertussis/genética , Bordetella parapertussis/patogenicidade , Bordetella pertussis/genética , Bordetella pertussis/patogenicidade , Campylobacter jejuni/genética , Campylobacter jejuni/patogenicidade , Helicobacter pylori/genética , Helicobacter pylori/patogenicidade , Análise de Sequência de DNA , Virulência/genética , Wolinella/genética , Wolinella/patogenicidadeRESUMO
To understand the origin and emergence of pathogenic bacteria, knowledge of the genetic inventory from their nonpathogenic relatives is a prerequisite. Therefore, the 2.11-megabase genome sequence of Wolinella succinogenes, which is closely related to the pathogenic bacteria Helicobacter pylori and Campylobacter jejuni, was determined. Despite being considered nonpathogenic to its bovine host, W. succinogenes holds an extensive repertoire of genes homologous to known bacterial virulence factors. Many of these genes have been acquired by lateral gene transfer, because part of the virulence plasmid pVir and an N-linked glycosylation gene cluster were found to be syntenic between C. jejuni and genomic islands of W. succinogenes. In contrast to other host-adapted bacteria, W. succinogenes does harbor the highest density of bacterial sensor kinases found in any bacterial genome to date, together with an elaborate signaling circuitry of the GGDEF family of proteins. Because the analysis of the W. succinogenes genome also revealed genes related to soil- and plant-associated bacteria such as the nif genes, W. succinogenes may represent a member of the epsilon proteobacteria with a life cycle outside its host.
Assuntos
Genoma Bacteriano , Wolinella/genética , Proteínas de Bactérias/metabolismo , Glicosilação , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Transdução de Sinais , Virulência/genética , Wolinella/metabolismo , Wolinella/patogenicidadeRESUMO
Recent microbiological studies of periodontal disease in humans have supported the concept of a specific bacterial etiology. While individual agents have not been unequivocally identified, numerous Gram-negative members of the subgingival microflora have been implicated. In addition, elevations in systemic antibody responses have been consistent with certain oral microorganisms being involved in an infectious process associated with the disease. This report delineates the relationship between elevated systemic antibody levels and oral colonization with the homologous microorganism at active disease sites. Thirty-four patients with various types of periodontal disease were examined. Using ELISA, each patient was shown to have an elevated antibody response to at least one organism from a battery of 18 oral microorganisms that were tested. Subsequently, subgingival plaque was cultured from disease-active and -inactive sites of each subject. The results demonstrated that the same microorganism to which the individual exhibited elevated serum antibody responses was detected in nearly 55% of the disease-active sites, while only 18% of the inactive sites showed the microorganism. Certain microorganisms including Actinobacillus actinomycetemcomitans, Bacteroides gingivalis, Eikenella corrodens and Wolinella recta were primarily or exclusively correlated with active disease lesions. These findings support the hypothesis that elevated systemic antibodies to periodontopathic bacteria are reflective of subgingival colonization and exist as a response to a bacterial infection at disease-active sites.
