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1.
Int J Syst Evol Microbiol ; 68(6): 2024-2027, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29683416

RESUMO

Two strains of a novel yeast species were isolated from traditional Chinese sauerkraut samples collected in Nanyang, Henan Province, central China. Phylogenetic analysis based on the concatenated sequences of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer (ITS) regions showed that these strains belong to the Yarrowia clade, with seven clones of uncultured Yarrowia as their closest phylogenetic neighbours. They differed from their closest known species, Yarrowia divulgata CBS 11013T, by 3.2 % sequence divergence (14 substitutions and 2 gaps) in the D1/D2 domains and by 5.4 % sequence divergence (12 substitutions and 5 gaps) in the ITS regions. The two strains of novel species reproduced asexually, and no ascospores could be found. The name Yarrowia brassicae f.a., sp. nov. is proposed to accommodate these strains, with NYNU 17218T (=CICC 33263T=CBS 15225T) as the type strain.


Assuntos
Alimentos Fermentados/microbiologia , Filogenia , Yarrowia/classificação , China , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Técnicas de Tipagem Micológica , Análise de Sequência de DNA , Esporos Fúngicos , Yarrowia/genética , Yarrowia/isolamento & purificação
3.
Nat Commun ; 8: 15186, 2017 05 09.
Artigo em Inglês | MEDLINE | ID: mdl-28485370

RESUMO

Insects that use ephemeral resources must rapidly digest nutrients and simultaneously protect them from competitors. Here we use burying beetles (Nicrophorus vespilloides), which feed their offspring on vertebrate carrion, to investigate the digestive and defensive basis of carrion utilization. We characterize gene expression and microbiota composition in the gut, anal secretions, and on carcasses used by the beetles. We find a strict functional compartmentalization of the gut involving differential expression of immune effectors (antimicrobial peptides and lysozymes), as well as digestive and detoxifying enzymes. A distinct microbial community composed of Firmicutes, Proteobacteria and a clade of ascomycetous yeasts (genus Yarrowia) is present in larval and adult guts, and is transmitted to the carcass via anal secretions, where the yeasts express extracellular digestive enzymes and produce antimicrobial compounds. Our results provide evidence of potential metabolic cooperation between the host and its microbiota for digestion, detoxification and defence that extends from the beetle's gut to its nutritional resource.


Assuntos
Besouros/microbiologia , Digestão , Microbioma Gastrointestinal , Trato Gastrointestinal/microbiologia , Canal Anal/microbiologia , Animais , Peptídeos Catiônicos Antimicrobianos/metabolismo , Feminino , Trato Gastrointestinal/anatomia & histologia , Trato Gastrointestinal/fisiologia , Perfilação da Expressão Gênica , Regulação da Expressão Gênica , Ontologia Genética , Imunidade Inata/genética , Inativação Metabólica/genética , Masculino , Muramidase/metabolismo , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Transcriptoma/genética , Yarrowia/isolamento & purificação , Yarrowia/metabolismo
4.
Med Mycol ; 55(2): 193-202, 2017 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-27440915

RESUMO

Yarrowia lipolytica is ubiquitous in the environment, opportunistic, and might be considered as one of the causative agents of catheter-related candidemia. Our work aimed to study some virulence factors of Y. lipolytica such as hydrolases production and biofilm formation with comparison to the most frequent Candida specie in human disease. In sum, 58 clinical isolates of Y. lipolytica, 16 C. glabrata, and 12 C. albicans were collected from Intensive care unit (ICU). All were tested for enzymatic production and biofilm formation. All tested isolates of C. albicans and C. glabrata were able to degrade casein, and 98.2% of Y. lipolytica showed caseinase activity but no gelatinase activity was detected in all isolates. Y. lipolytica strains showed significantly lower (3.4%) in vitro phospholipase activity than C. albicans and C. glabrata (P < .05). No significant differences of the hemolytic activity were detected between the three species (P > .05). Concerning biofilm formation, and unlike the results obtained on polystyrene plate, the number of adhered and biofilm cultivable cells obtained by Y. lipolytica after 168 hours of catheter subcutaneous implantation is significantly greater and tends to be more compact and structured hyphal layer. Although C. albicans remains the most pathogenic yeast, development of selective ability of Y. lipolytica to adhere, to form a biofilm on catheter medical devices, and to produce phospholipase and hemolytic enzyme is of particular interest, and it is strongly recommended to be vigilant in the use of medical implanted medical devices, particularly in ICU.


Assuntos
Biofilmes/crescimento & desenvolvimento , Fungemia/microbiologia , Fatores de Virulência/análise , Yarrowia/fisiologia , Yarrowia/patogenicidade , Adulto , Animais , Candida/isolamento & purificação , Candida/patogenicidade , Candida/fisiologia , Catéteres/microbiologia , Feminino , Proteínas Hemolisinas/análise , Humanos , Hidrolases/análise , Masculino , Modelos Animais , Ratos , Yarrowia/isolamento & purificação
5.
Bioresour Technol ; 221: 234-240, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27639676

RESUMO

The objective of this work was to study the kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7. The model terms glycerol concentration inoculum and C/N ratio with inoculum were found to be significant for lipid production. Lipid production was found to be higher in glycerol 82.5g/L, C/N ratio 75 and inoculum volume 6.25%. Optimized culture conditions were tested at 15L bench scale reactor. The biomass concentration and lipid content obtained was 29.5g/L and 50% (w/w), respectively. The yield coefficients were calculated and found to be 0.332g/g (g biomass/g of glycerol) of biomass and 0.179g/g (g lipid/g glycerol consumed) for lipid. Observed rates of lipid production show lipid production from 30h of fermentation. Out of the total glycerol consumed, 41.1% glycerol was converted into biomass, lipid, and citric acid.


Assuntos
Lipídeos/biossíntese , Yarrowia/metabolismo , Biocombustíveis , Biomassa , Reatores Biológicos/microbiologia , Ácido Cítrico/metabolismo , Ácidos Graxos não Esterificados/metabolismo , Fermentação , Glicerol/metabolismo , Cinética , Metanol/metabolismo , Yarrowia/crescimento & desenvolvimento , Yarrowia/isolamento & purificação
6.
Mycopathologia ; 179(5-6): 437-45, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25614084

RESUMO

Yarrowia lipolytica is weakly pathogenic yeast, which is rarely isolated from the blood. We report unusual cases of Y. lipolytica fungemia occurred between October 2012 and June 2014 in the intensive care unit (ICU) of the UH Habib Bourguiba Sfax. During this period, 55 cases of Y. lipolytica septicemia were diagnosed. There were 44 men and 11 women (sex ratio = 4).The median age was 43 years. The broad-spectrum antibiotics (100 %), the catheterization (96 %), and the prolonged hospitalization in ICU (91 %) were the main risk factors. Patients were hospitalized in ICU, mostly, for polytraumatism (45.4 %), pneumopathy (9 %), and post-operative complications (7 %). Fever unresponsive to broad-spectrum antibacterial therapy was the predominant sign of infection (83.6 %). Y. lipolytica was isolated in one or several blood cultures (14.5 %) and in the catheter tip culture of nine patients (16.3 %).Treatment was based on intravenous amphotericin B (58.2 %), fluconazole (45.4 %) and/or removal catheter (69 %). Apyrexia or blood cultures sterilization was obtained for 34 patients (61.8 %). Y. lipolytica candidemia is an opportunistic and emerging human yeast pathogen. It can reach to the bloodstream of immunocompromised or critically ill patients during hospitalization through intravascular catheterization. Further clinical data need to be evaluated for formulating management strategies of seriously ill patients infected with uncommon fungal agents.


Assuntos
Fungemia/diagnóstico , Fungemia/microbiologia , Yarrowia/isolamento & purificação , Adulto , Idoso , Antifúngicos/uso terapêutico , Feminino , Fungemia/tratamento farmacológico , Hospitalização , Humanos , Masculino , Pessoa de Meia-Idade , Fatores de Risco , Resultado do Tratamento , Tunísia
7.
Appl Biochem Biotechnol ; 175(4): 1926-37, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25432337

RESUMO

In this study, nine heterotrophic yeast isolates were tested for their ability to assimilate crude glycerol and consecutive conversion to triacylglycerides (TGAs). All the organisms were initially screened on crude glycerol-based selection media, and those producing lipid globules were further evaluated for lipid production. Sudan Black B staining of eight isolates showed lipid globules. These strains were further studied at different C/N ratio. The molecular identification revealed that the isolates belonged to the genera of Yarrowia and Candida. Among these isolates, SKY7 (Yarrowia lipolytica) produced up to 42.04 ± 0.11 % of lipid w/w) with a C/N ratio of 100 and fermentation time of 72 h. The other strains produced 5.82 ± 0.4 to 34.57 ± 0.44 % lipid (w/w). The GC-flame ionization detector (FID) lipid profile showed that the lipid produced by the strains had close resemblance with vegetable oil and could serve as a feedstock for biodiesel production. Biolog test of the isolates revealed a wide spectrum of carbon utilization.


Assuntos
Candida/metabolismo , RNA Ribossômico 18S/genética , Microbiologia do Solo , Triglicerídeos/biossíntese , Yarrowia/metabolismo , Aminoácidos/metabolismo , Compostos Azo , Biocombustíveis , Candida/classificação , Candida/genética , Candida/isolamento & purificação , Meios de Cultura/química , Fermentação , Glicerol/metabolismo , Monossacarídeos/metabolismo , Técnicas de Tipagem Micológica , Naftalenos , Oligossacarídeos/metabolismo , Filogenia , Solo/química , Yarrowia/classificação , Yarrowia/genética , Yarrowia/isolamento & purificação
8.
Appl Microbiol Biotechnol ; 99(8): 3651-7, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25520168

RESUMO

Difference in mercuric ion removal by resting and growing cells of two mercury-resistant yeast strains, identified as Yarrowia spp. (strains Idd1 and Idd2), were studied. Resting cells of strain Idd2 exhibited high maximum Hg(2+) removal capacity (59 mg mercury per g dry cell weight [gdw(-1)]) by adsorption than those of resting cells of strain Idd1 (32 mg gdw(-1)). The resting cells of strain Idd2 exhibited a higher Hg(2+) desorption capacity using CaCl2 (68 %) and EDTA (48 %) than strain Idd1, depicting weaker binding of Hg(2+) onto strain Idd2 unlike strain Idd1. The actively growing yeast cells showed opposite Hg removal characteristics to those of the resting cells. Strain Idd1 adsorbed less Hg(2+) from culture medium supplemented with Hg(2+) than strain Idd2. However, the growing strain Idd1 reduced and vaporized 27 % of supplemented Hg(2+) as metallic mercury (Hg(0)), while the growing strains Idd2 vaporized 15 % of the supplemented Hg(2+). These two yeast strains are potential biotechnological tools for the eventual bioremediation of polluted aquatic systems.


Assuntos
Sedimentos Geológicos/microbiologia , Mercúrio/metabolismo , Poluentes da Água/metabolismo , Yarrowia/metabolismo , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Estuários , Genes de RNAr , Dados de Sequência Molecular , Oxirredução , RNA Fúngico/genética , RNA Ribossômico 18S/genética , Análise de Sequência de DNA , Yarrowia/classificação , Yarrowia/genética , Yarrowia/isolamento & purificação
9.
Antonie Van Leeuwenhoek ; 105(4): 697-707, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24500004

RESUMO

Eleven yeast strains representing two hitherto undescribed species were isolated from different kinds of meat samples in Hungary and one from the sediment of a tropical freshwater river in Southeastern Brazil. The analysis of the sequences of their large subunit rRNA gene D1/D2 domain and the internal transcribed spacer (ITS) regions placed the two new species in the Yarrowia clade. Some of the seven strains representing the first new species can mate and give rise to asci and form ascospores embedded in capsular material, which qualifies it as the third teleomorph species of the Yarrowia clade. The name Yarrowia porcina sp. nov. (type strain: NCAIM Y.02100(T) = CBS 12935(T) = NRRL Y-63669(T), allotype strain UFMG-RD131(A) = CBS 12932(A)) is proposed for this new yeast species, which, based on physiological characters, is indistinguishable from Yarrowia lipolytica and some other species of the genus. Considerable intraspecific variability was detected among the sequences of the large subunit rRNA gene D1/D2 domains of the seven strains. The variability among the D1/D2 sequences exceeded the divergence observed among the ITS sequences and in some cases more than 1 % substitution among the D1/D2 sequences was detected. The conspecificity of these strains was supported by the low (0-3 substitutions) sequence divergence among their ITS sequences, the result of a parsimony network analysis utilizing the concatenated ITS and D1/D2 sequences and also by the fingerprint patterns generated by microsatellite primed PCR. No ascospore formation was observed in the group of the other five strains representing the second new species. These strains shared identical D1/D2 and ITS sequences. Yarrowia bubula f.a., sp. nov. (type strain: NCAIM Y.01998(T) = CBS 12934(T) = NRRL Y-63668(T)) is proposed to accommodate these strains.


Assuntos
Carne/microbiologia , Rios/microbiologia , Yarrowia/classificação , Yarrowia/isolamento & purificação , Brasil , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Genes de RNAr , Sedimentos Geológicos/microbiologia , Hungria , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Filogenia , RNA Fúngico/genética , RNA Ribossômico/genética , Análise de Sequência de DNA , Yarrowia/genética
10.
Int J Syst Evol Microbiol ; 63(Pt 12): 4818-4823, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24158944

RESUMO

Five yeast strains, phenotypically indistinguishable from Yarrowia lipolytica and Yarrowia deformans, were recovered from different animal-related samples. One strain was isolated from a bacon processing plant in Denmark, two strains from chicken liver in the USA, one strain from chicken breast in Hungary and one from minced beef in Hungary. Comparisons of the sequences of their large subunit rRNA gene D1/D2 domain and the internal transcribed spacer (ITS) regions revealed that, despite their phenotypic similarity, they represent a novel yeast species of the Yarrowia clade with Y. deformans being the genotypically closest relative (LSU rRNA gene D1/D2 and ITS region similarity of 97.0 and 93.7 %, respectively). Yarrowia divulgata f.a., sp. nov. is proposed to accommodate these strains with F6-17(T) ( = CBS 11013(T) = CCUG 56725(T)) as the type strain. Some D1/D2 sequences of yeasts from marine habitats were found in the GenBank database that were identical to those of the strains of Y. divulgata f.a., sp. nov. Unfortunately, these strains were not available for our study.


Assuntos
Carne/microbiologia , Filogenia , Yarrowia/classificação , Animais , Bovinos , Galinhas , DNA Fúngico/genética , DNA Espaçador Ribossômico/genética , Dinamarca , Hungria , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Subunidades Ribossômicas Menores de Eucariotos/genética , Análise de Sequência de DNA , Suínos , Estados Unidos , Yarrowia/genética , Yarrowia/isolamento & purificação
11.
Antonie Van Leeuwenhoek ; 104(6): 1117-23, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24026513

RESUMO

Ascomycetous yeast strain SM-22 was isolated from the sea-surface microlayer near the Keelung City off the northern coast of Taiwan. This strain showed a cell surface hydrophobicity higher than 90 %, moderate UV A/B resistance, and it degraded 68 % of the total petroleum hydrocarbon content of an artificial seawater medium containing 1 % (v v(-1)) diesel oil within 15 days at 25 °C. The closest phylogenetic relative of this strain is Candida oslonensis CBS 10146(T), but it differs from strain SM-22 by a 3.7 % divergence (including 18 nucleotide substitutions and 2 gaps) in the D1/D2 domain sequence of the large subunit rRNA gene. This difference clearly suggests that the strain SM-22 represents a distinct species. Strain SM-22 does not produce ascospores on common sporulation media and it can therefore be considered an anamorph of the genus Yarrowia. Thus, the name Yarrowia keelungensis sp. nov. (type strain SM-22(T) = BCRC 23110(T) = JCM 14894(T) = CBS 11062(T)) is proposed as a novel species of genus Yarrowia.


Assuntos
Óleos/metabolismo , Água do Mar/microbiologia , Yarrowia/classificação , Yarrowia/isolamento & purificação , Biotransformação , Análise por Conglomerados , Meios de Cultura/química , DNA Fúngico/química , DNA Fúngico/genética , DNA Intergênico/química , DNA Intergênico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Genes de RNAr , Microscopia , Dados de Sequência Molecular , Técnicas de Tipagem Micológica , Filogenia , RNA Fúngico/genética , RNA Ribossômico/genética , Análise de Sequência de DNA , Esporos Fúngicos/crescimento & desenvolvimento , Taiwan , Yarrowia/crescimento & desenvolvimento , Yarrowia/metabolismo
12.
Bioresour Technol ; 137: 124-31, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23587815

RESUMO

This study demonstrated the potential applicability of the isolated strains of Yarrowia lipolytica for the valorization of glycerol waste generated during biodiesel production, throughout biomass production. Twenty-one strains were isolated from different environments and identified as Y. lipolytica. Biomass production from pure glycerol (25 g L(-1)) was performed in the shake-flasks experiment. Eight strains with the best biomass production ability were chosen for studies in bioreactor (pH 3.5). The analysis of technological process parameters and biomass chemical composition demonstrated that S6 strain was the most suitable for biomass production. Its application allowed obtaining 11.7 and 12.3 g L(-1) of the biomass with 1.30 and 1.37 g L(-1) h(-1) productivity, respectively when pure and raw glycerol (25 g L(-1)) was used. In the yeast protein amino acid profile the contents of lysine, threonine and phenylalanine/tyrosine were higher than required by FAO/WHO. According to the EAAI, the nutritional value of the biomass reached up to 72.3%.


Assuntos
Biocombustíveis , Biomassa , Glicerol/metabolismo , Yarrowia/metabolismo , Yarrowia/classificação , Yarrowia/isolamento & purificação
13.
Bioresour Technol ; 123: 636-45, 2012 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-22960123

RESUMO

An extracellular laccase-producing yeast was isolated from soil and identified as Yarrowia lipolytica by its morphology and by comparison of its internal transcribed spacer rDNA gene sequence. Extracellular laccase (YlLac) from Y. lipolytica was purified to homogeneity by anion-exchange and gel filtration chromatography. YlLac is a monomeric glycoprotein with 14% carbohydrate content and a molecular mass of 67kDa. It showed a higher catalytic efficiency towards 2,2'-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (k(cat)/K(m)=19.3s(-1)µM(-1)) and 2,6-dimethoxyphenol (k(cat)/K(m)=13s(-1)µM(-1)) than any other reported laccase. This enzyme was able to oxidize phenolic compounds present in pretreated rice straw. Several parameters (temperature, enzyme concentration, and mediator compounds) to enhance removal of phenolic compounds from pretreated rice straw were optimized using response surface methodology. The use of YlLac for the removal of cellulase inhibitory compounds from biomass slurries was found to be a promising approach for improving the efficiency of biorefineries.


Assuntos
Lacase/metabolismo , Oryza/química , Fenóis/isolamento & purificação , Resíduos/análise , Yarrowia/enzimologia , Sequência de Aminoácidos , Biodegradação Ambiental/efeitos dos fármacos , Metabolismo dos Carboidratos/efeitos dos fármacos , Celulase/metabolismo , Ativação Enzimática/efeitos dos fármacos , Inibidores Enzimáticos/farmacologia , Evolução Molecular , Hidrólise/efeitos dos fármacos , Íons , Cinética , Lacase/isolamento & purificação , Metais/farmacologia , Dados de Sequência Molecular , Peptídeos/química , Filogenia , Reprodutibilidade dos Testes , Especificidade por Substrato/efeitos dos fármacos , Yarrowia/isolamento & purificação
14.
Braz. j. microbiol ; 43(2): 432-440, Apr.-June 2012. graf, tab
Artigo em Inglês | LILACS | ID: lil-644456

RESUMO

The work herewith investigated the production of yeast biomass as a source of protein, using Yarrowia lipolytica NRRL YB-423 and raw glycerol from biodiesel synthesis as the main carbon source. A significant influence of glycerol concentration, initial pH and yeast extract concentration on biomass and protein content was observed according to the 2v5-1 fractional design. These factors were further evaluated using a central composite design and response surface methodology, and an empirical model for protein content was established and validated. The biomass of Yarrowia lipolytica NRRL YB-423 reached 19.5 ± 1.0 g/L in shaken flasks cultivation, with a protein content of 20.1 ± 0.6% (w/w).


Assuntos
Glicerol/análise , Glicerol/isolamento & purificação , Leveduras/isolamento & purificação , Yarrowia/isolamento & purificação , Métodos
15.
Mar Pollut Bull ; 64(7): 1386-91, 2012 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-22622152

RESUMO

Among six crude oil-degrading yeasts that were isolated from an oil-polluted area in the Persian Gulf, two yeast strains showed high degradation activity of aliphatic hydrocarbons. From an analysis of 18S rRNA sequences and biochemical characteristics, these strains were identified as Yarrowia lipolytica strains PG-20 and PG-32. Gas Chromatography (GC) analysis of the crude oil remaining in the culture medium after 1 week at 30°C showed that the strains PG-20 and PG-32 degraded 68% and 58% of crude oil, respectively. The optimal growth condition and biodegradation of hydrocarbons was in ONR medium with an acidic pH (pH5). These two strains may degrade aliphatic hydrocarbons more efficiently than aromatic hydrocarbons, although strain PG-20 had better degradation than strain PG-32. The two Y. lipolytica strains reduce surface tension when cultured on hydrocarbon substrates (1% v/v). These strains showed a cell surface hydrophobicity higher than 70%. These results suggested that Y. lipolytica strains PG-20 and PG-32 have high crude oil degrading activity due to their high emulsifying activity and cell hydrophobicity. In conclusion, these yeast strains can be useful for the bioremediation process in the Persian Gulf and decreasing oil pollution in this marine ecosystem.


Assuntos
Petróleo/metabolismo , Água do Mar/microbiologia , Poluentes Químicos da Água/metabolismo , Yarrowia/metabolismo , Biodegradação Ambiental , Oceano Índico , Petróleo/análise , Microbiologia da Água , Poluentes Químicos da Água/análise , Yarrowia/classificação , Yarrowia/genética , Yarrowia/isolamento & purificação
16.
Yeast ; 28(10): 721-32, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21905092

RESUMO

Yarrowia lipolytica 3589, a tropical marine yeast, grew aerobically on a broad range of bromoalkanes varying in carbon chain length and differing in degree and position of bromide group. Amongst the bromoalkanes studied, viz. 2-bromopropane (2-BP), 1-bromobutane (1-BB), 1,5-dibromopentane (1,5-DBP) and 1-bromodecane (1-BD), the best utilized was 1-BD, with a maximal growth rate (µ(max) ) of 0.055 h⁻¹ and an affinity ratio (µ(max) /K(s) ) of 0.022. Utilization of these bromoalkanes as growth substrates was associated with a concomitant release of bromide (8202.9 µm) and cell mass (36 × 109 cells/ml), occurring maximally on 1-BD. Adherence of yeast cells to these hydrophobic bromoalkanes was observed microscopically, with an increase in cell size and surface hydrophobicity. The maximal cell diameter was for 1-BD (4.66 µm), resulting in an increase in the calculated cell surface area (68.19 µm²) and sedimentation velocity (1.31 µm/s). Cell surface hydrophobicity values by microbial adhesion to solvents (MATS) analysis for yeasts grown on bromoalkanes and glucose were significantly high, i.e. >80%. Similarly, water contact angles also indicate that the cell surface of yeast cells grown in glucose possess a relatively more hydrophilic cell surface (θ = 49.1°), whereas cells grown in 1-BD possess a more hydrophobic cell surface (θ = 90.7°). No significant change in emulsification activity or surface tension was detected in the cell-free supernatant. Thus adherence to the bromoalkane droplets by an increase in cell size and surface hydrophobicity leading to debromination of the substrate might be the strategy employed in bromoalkane utilization and growth by Y. lipolytica 3589.


Assuntos
Água do Mar/microbiologia , Yarrowia/química , Yarrowia/crescimento & desenvolvimento , Hidrocarbonetos Bromados/metabolismo , Interações Hidrofóbicas e Hidrofílicas , Índia , Propriedades de Superfície , Yarrowia/isolamento & purificação , Yarrowia/metabolismo
17.
Meat Sci ; 89(1): 21-6, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-21497447

RESUMO

The effects of selected variables, i.e. temperature, water activity and yeast inoculation level, on the lipolytic pattern and volatile production by Yarrowia lipolytica Y16A (chosen on the basis of a previous screening) were assessed. The variables were varied according to a central composite design and the models obtained enabled evaluation and weighting of the effects of the independent variables on the free fatty acids (FFAs) and volatile profiles in pork fat based medium. The polynomial models showed the levels temperature, water activity of the pork fat based system and yeast strain inoculation were able to maximize the release of specific FFAs or molecules of sensory importance.


Assuntos
Microbiologia de Alimentos , Lipólise , Carne/microbiologia , Compostos Orgânicos Voláteis/metabolismo , Yarrowia/enzimologia , Animais , Ácidos Graxos não Esterificados , Cromatografia Gasosa-Espectrometria de Massas , Modelos Estatísticos , Suínos , Temperatura , Yarrowia/crescimento & desenvolvimento , Yarrowia/isolamento & purificação
18.
Meat Sci ; 88(4): 689-93, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21454020

RESUMO

This work studied the variability in lipolytic activity in 35 strains of Yarrowia lipolytica inoculated in pork fat after 7 and 21 days of storage at 15 °C. The strains were able to generate three different hydrolysis profiles. In particular, the strains PO10, PO14, RO1, RO5, Y15, Y16A, Y20, B5, 7B, 7B3, 16B and 21C caused an increase with time in concentrations of C16:0, C18:0, C18:1, C18:1(Δ11) and C18:2 which were the predominant free fatty acids (FFAs). On the contrary, the strains PO1, PO19, PO23, RO22, Y12, B4, B74, GB, 5B, 5D, 27D and W29 showed an opposite trend, while the remaining ones induced no change. Because the released FFAs can be considered precursors for flavour development, the results suggest the potential use of some Y. lipolytica strains in sausage making to improve the overall aroma.


Assuntos
Gorduras na Dieta/análise , Carne/microbiologia , Yarrowia/crescimento & desenvolvimento , Yarrowia/isolamento & purificação , Animais , Meios de Cultura , Ácidos Graxos não Esterificados/metabolismo , Microbiologia de Alimentos , Metabolismo dos Lipídeos , Lipólise , Suínos
19.
J Pediatr Hematol Oncol ; 33(3): e120-1, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21383637

RESUMO

Yarrowia lipolytica is a weakly pathogenic yeast that is rarely isolated from the blood. We treated a patient with acute lymphoblastic leukemia who underwent catheter-related fungemia attributable to this organism. The patient was recovered after 3 day's prophylactic dose of fluconazole and removal of deep venous catheter. Yarrowia lipolytica was also identified from yeast extract capsule that was administered to this child. This case indicated that it is necessary to give antifungal therapy and to remove catheter in catheter-related fungemia, and immuno-compromised patient's diet and medication should be managed strictly.


Assuntos
Fungemia/etiologia , Leucemia-Linfoma Linfoblástico de Células Precursoras/complicações , Yarrowia/isolamento & purificação , Adolescente , Infecções Relacionadas a Cateter/etiologia , Fungemia/tratamento farmacológico , Humanos , Masculino
20.
Lett Appl Microbiol ; 51(3): 264-71, 2010 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-20636327

RESUMO

AIMS: To obtain a thiamine-auxotrophic yeast strain that overproduces alpha-ketoglutaric acid (alpha-KG) from glycerol and to investigate nutrient effects on alpha-KG production. METHODS AND RESULTS: Yeast strain WSH-Z06, a thiamine auxotroph that gave high yields of alpha-KG from glycerol, was obtained by screening for ampicillin/kanamycin resistance and thiamine auxotrophy. The strain was identified as Yarrowia lipolytica based on physiological, chemical, and phylogenetic analysis. The ability of the strain to convert glycerol to alpha-KG was analysed by investigating the effects of nutritional factors, including thiamine, riboflavin, nitrogen sources, and calcium ion. CONCLUSIONS: Thiamine and calcium ion concentration had the greatest effect on alpha-KG accumulation. Under optimal conditions, a yield of 39.2 g l(-1)alpha-KG was obtained from 100 g l(-1) glycerol, with 16.84 g l(-1) pyruvate as a by-product. SIGNIFICANCE AND IMPACT OF THE STUDY: The current work provides a method for screening for an alpha-KG overproducer. Nutrients have a significant impact on alpha-KG production in the yeast strain presented here. The alpha-KG-overproducing yeast strain Y. lipolytica WSH-Z06 is a promising parent strain for further metabolic engineering to lower by-product accumulation and accelerate glycerol utilization.


Assuntos
Glicerol/metabolismo , Ácidos Cetoglutáricos/metabolismo , Tiamina/metabolismo , Yarrowia/genética , Yarrowia/metabolismo , Cálcio/metabolismo , Análise por Conglomerados , DNA Fúngico/química , DNA Fúngico/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Genes de RNAr , Técnicas de Tipagem Micológica , Nitrogênio/metabolismo , Filogenia , Ácido Pirúvico/metabolismo , RNA Fúngico/genética , RNA Ribossômico/genética , Riboflavina/metabolismo , Análise de Sequência de DNA , Yarrowia/classificação , Yarrowia/isolamento & purificação
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