Comparative Transcriptomic Analyses of Different Jujube Cultivars Reveal the Co-Regulation of Multiple Pathways during Fruit Cracking.

Fruit cracking is a common physiological disorder in many fruit species. Jujube (Ziziphus jujuba Mill.) is an economically valuable fruit in which fruit cracking seriously affects fruit yield and quality and causes significant economic losses. To elucidate cracking-related molecular mechanisms, the cracking-susceptible cultivars 'Cuizaohong' and 'Jinsixiaozao' and the cracking-resistant cultivar 'Muzao' were selected, and comparative transcriptome analyses of cracking and non-cracking 'Cuizaohong' (CC and NC), cracking and non-cracking 'Jinsixiaozao' (CJ and NJ), and non-cracking 'Muzao' (NM) were conducted. A total of 131 differentially expressed genes (DEGs) were common to the CC vs. NC and CJ vs. NJ comparisons. To avoid passive processes after fruit cracking, we also mainly focused on the 225 gradually downregulated DEGs in the CJ, NJ, and NM samples. The functional annotation of the candidate DEGs revealed that 61 genes related to calcium, the cell wall, the cuticle structure, hormone metabolism, starch/sucrose metabolism, transcription factors, and water transport were highly expressed in cracking fruits. We propose that expression-level changes in these genes might increase the turgor pressure and weaken mechanical properties, ultimately leading to jujube fruit cracking. These results may serve as a rich genetic resource for future investigations on fruit cracking mechanisms in jujube and in other fruit species.

Jujube witches' broom phytoplasma effectors SJP1 and SJP2 induce lateral bud outgrowth by repressing the ZjBRC1-controlled auxin efflux channel.

Comprehensively controlling phytoplasma-associated jujube witches' broom (JWB) disease is extremely challenging for the jujube industry. Although the pathogenesis of phytoplasma disease has been highlighted in many plant species, the release of lateral buds from dormancy under JWB phytoplasma infection has not been characterized in woody perennial jujube. Here, two 16SrV-B group phytoplasma effectors, SJP1 and SJP2, were experimentally determined to induce witches' broom with increased lateral branches. In vivo interaction and subcellular localization analyses showed that both SJP1 and SJP2 were translocated from the cytoplasm to the nucleus to target the CYC/TB1-TCP transcription factor ZjBRC1. The N- and C-terminal coiled-coil domains of SJP1 and SJP2 were required for the TCP-binding ability. ZjBRC1 bound directly to the auxin efflux carrier ZjPIN1c/3 promoters and down-regulated their expression to promote the accumulation of endogenous auxin indole-3-acetic acid in jujube calli. Furthermore, JWB phytoplasma infection suppressed ZjBRC1 accumulation and induced ZjPIN1c/3 expression to stimulate lateral bud outgrowth. Therefore, SJP1 and SJP2 stimulate lateral bud outgrowth, at least partly, by repressing the ZjBRC1-controlled auxin efflux channel in jujube, representing a potential strategy for comprehensive phytoplasma-associated disease control and a resource for gene editing breeding to create new cultivars with varying degrees of shoot branching.

Application of artificial neural network and support vector regression in predicting mass of ber fruits (Ziziphus mauritiana Lamk.) based on fruit axial dimensions.

Fruit quality attributes are important factors for designing a market for agricultural goods and commodities. Support vector regression (SVR), MLR, and ANN models were established to predict the mass of ber fruits (Ziziphus mauritiana Lamk.) based on the axial dimensions of the fruit from manual measurements of fruit length, minor fruit diameter, and maximum fruit diameter of four ber cultivars. The precision and accuracy of the established models were assessed given their predicted values. The results revealed that using the validation dataset, the developed ANN (R2 = 0.9771; root mean square error [RMSE] = 1.8479 g) and SVR (R2 = 0.9947; RMSE = 1.8814 g) models produced better results when predicting ber fruit mass than those obtained by the MLR model (R2 = 0.4614; RMSE = 11.3742 g). In estimating ber fruit mass, the established SVR and ANN models produced more precise prediction values than those produced by the MLR model; however, the performance differences between the SVR and ANN models were not clear.

Isolation and characterization of endophytic bacteria for controlling root rot disease of Chinese jujube.

AIM: Fusarium oxysporum is the primary pathogen causing root rot disease that severely affects cultivation of jujube fruit in the Xinjiang province of China. The aim of this study was to identify endophytic bacteria in healthy jujube organs that could effectively suppress F. oxysporum growth. METHODS AND RESULTS: Different plant organs (leaves, twigs and roots) were collected from healthy Chinese jujube cultivated in southern Xinjiang province of China. The endophytic bacterium Brevibacterium halotolerans JZ7 was selected for its strong antagonistic activity and growth-promoting characteristics. Gas-chromatography mass-spectrometry analysis showed that acetoin, 2,3-butanediol and fenretinide were the three dominant volatile organic compounds produced by strain JZ7. Fenretinide strongly suppressed spore germination of F. oxysporum in vitro. Pot experiments showed that strain JZ7 colonized both the roots and rhizosphere soil of Chinese jujube and significantly reduced F. oxysporum level in jujube rhizosphere soil. CONCLUSION: We demonstrated that B. halotolerans JZ7 can be developed into a biological control agent to combat root rot disease of Chinese jujube in the Xinjiang province of China. SIGNIFICANCE AND IMPACT OF THE STUDY: The suggested strategy for biological control of jujube root rot disease is fully in accordance with the current principles of sustainability.

Metabolomic and Transcriptomic Analyses of Anthocyanin Biosynthesis Mechanisms in the Color Mutant Ziziphus jujuba cv. Tailihong.

The purplish-red color of "Tailihong" jujube fruit skins is caused primarily by anthocyanin accumulation, but the mechanisms that underlie anthocyanin biosynthesis in jujube fruit have rarely been studied. We performed metabolomic and transcriptomic analyses of jujube fruit skins at different developmental stages and identified a total of 158 flavonoids, among which cyanidin-3-O-rutinoside and peonidin-3,5-O-diglucoside were the primary anthocyanins. During fruit development and maturation, the anthocyanin content was strongly correlated with the expression of ZjANS and ZjUGT79B1, suggesting that these are key genes in the anthocyanin biosynthesis process. Transcriptomic analysis indicated that the transcription factors ZjMYB5, ZjTT8, and ZjWDR3 regulated anthocyanin biosynthesis in jujube fruit skins. Subcellular localization experiments confirmed that ZjANS and ZjUGT79B1 were localized to the nucleus and the endoplasmic reticulum. ZjMYB5 and ZjTT8 were found only in the nucleus, whereas strong fluorescence signals from ZjWDR3 were observed in the nucleus and cytoplasm. Prokaryotic expression and in vitro enzyme activity assays showed that the recombinant ZjANS protein catalyzed the formation of cyanidin from (+)-catechin. Secondary glycosylation by ZjUFGT79B1 modified cyanidin-3-O-glucoside to produce cyanidin-3-O-rutinoside, and ZjCCoAOMT readily catalyzed the production of the methylated anthocyanin peonidin-3,5-O-diglucoside from cyanidin 3,5-O-glucoside. Dual-Luciferase and GUS activity assays showed that the ZjANS and ZjUGT79B1 promoters were activated by ZjMYB5, ZjTT8, and ZjWDR3. All data indicated that these three transcription factors played important roles in anthocyanin biosynthesis in the color mutant Ziziphus jujuba cv. Tailihong, contributing to anthocyanin accumulation by enhancing the expression of ZjANS and ZjUGT79B1.

Effect of leaf type on browse selection by free-ranging goats in a southern African savanna.

Broad- and fine-leaved woody species respond to seasonal changes from wet to dry season differently. For example, broad-leaved species shed their leaves earlier, while fine-leaved species, especially acacias retain green foliage well into the dry season. These differences are expected to result in variation in selection of broad- and fine-leaved woody species as browse by free-ranging goats. We tested the hypothesis that free-ranging goats select broad-leaved woody species more than fine-leaved species during wet (growth) season and fine-leaved woody species more than broad-leaved species during dry season. In addition, we tested if broad- and fine-leaved woody species had different foliar dry matter digestibility and chemical composition (crude protein, neutral detergent fibre, acid detergent fibre, total phenolics and condensed tannins concentration). Free-ranging goats were observed foraging on broad- and fine-leaved woody species over a two-year period (2014 and 2015) during three seasons: early wet (October/November), late wet (February/March) and dry (May/June). Ivlev's selectivity or Jacob's index (Ei) was calculated for five woody species (two broad-leaved and three fine-leaved) browsed by goats during wet and dry season. Jacob's selectivity index was higher for broad-leaved (Ziziphus mucronata and Searsia (Rhus) tenuinervis) than fine-leaved woody species (Acacia nilotica, Acacia karroo and Dichrostachys cinerea) during wet season. However, the trend was reversed during dry season with fine-leaved species having higher Jacob's selectivity index than broad-leaved species. Leaf dry matter digestibility and chemical composition was similar between broad- and fine-leaved woody species throughout the year. We conclude that goats selected broad-leaved woody species during wet season when browse was plentiful and then switched to fine-leaved species which retained leaves during dry season.

Molecular role of ethylene in fruit r​ipening of Ziziphus jujube Mill.

The fruit of Chinese jujube (Ziziphus jujube) is widely consumed by human beings due to its high proteins, vitamins, and mineral nutrients. The harvest time of Chinese jujube fruit determines its quality, while ethylene plays a pivotal role in fruit ripening. Nevertheless, the relationship between ethylene biosynthesis/signal transduction and fruit ripening of Chinese jujube is still elusive. Here, the Chinese jujube fruit ripening with its fruit peel color change from cyan to dark red at seven different ripening stages (stage I-VII) and expression levels of genes related to ethylene synthesis and signal transduction were determined. Results showed that expression levels of ZjACO1-3, ZjETR2, ZjERF1, and ZjERF4 were increasingly upregulated, whereas the expression levels of ZjERS1, ZjETI, ZjERF2, and ZjERF3 were downregulated from green to red fruit ripening stages. Among them, ZjACO1-3 promoters contain ethylene response element. Taken together, Chinese jujube fruit ripening might be affected by the ethylene signaling which was mainly regulated by ZjACO, a gene involved in ethylene biosynthesis. This research supports theories and techniques for the storage, preservation and molecular breeding of Z. jujube.

Elucidating the limiting factors for regeneration and successful establishment of the thermophilic tree Ziziphus spina-christi under a changing climate.

Due to climate change, winter temperatures are predicted to increase worldwide. For thermophilic trees, highly sensitive to low temperatures, an increase in winter temperatures may be beneficial for survival and regeneration. Ziziphus spina-christi is a thermophilic tree that has recently become more abundant and widespread in the eastern Mediterranean, presumably due to a gradual increase in winter temperatures. We aim to define the temperature limitations for seed germination and the growth and survival of young seedlings to broaden our understanding of the future geographical distribution of this species. We studied effects of temperature on germination, growth, and photosynthesis in a controlled environment with four different day/night temperature regimes (34/28 °C, 28/22 °C, 22/16 °C and 16/10 °C). Effects of endocarp on germination and seed germination in the field were also studied. Results showed that germination has a lower thermal optimum (34-22 °C, 63.5-67.5% germination) than growth and photosynthesis (34-28 °C). Moderate cold stress (22/16 °C), did not affect germination capacity, but strongly reduced seedling growth (71%) and photosynthetic capacity (44.6%). Under severe cold stress (16/10 °C), germination still occurs (22%), but seedlings cannot perform growth and photosynthesis. We conclude that slow seedling growth, not germination, is the main barrier for successful establishment of Z. spina-christi under low temperature. Warmer winters could lead to earlier establishment of seedlings and increase their chance of survival the following summer. This may explain the recent increase in the tree's relative abundance and further highlight the potential spread of this species at higher altitudes and latitudes across the Mediterranean.

Comparative transcriptome analysis uncovers different heat stress responses in heat-resistant and heat-sensitive jujube cultivars.

Jujube (Ziziphus jujuba Mill.) is an economically and agriculturally significant fruit crop and is widely cultivated throughout the world. Heat stress has recently become a primary abiotic stressor limiting the productivity and growth of jujube, as well as other crops. There are few studies, however, that have performed transcriptome profiling of jujube when it is exposed to heat stress. In this study, we observed the physiochemical changes and analyzed gene expression profiles in resistant jujube cultivar 'HR' and sensitive cultivar 'HS' subjected to heat stress for 0, 1, 3, and 5d. Twenty-four cDNA libraries from 'HR' and 'HS' leaves were built with a transcriptome assay. A total of 6887 and 5077 differentially expressed genes were identified in 'HR' and 'HS' after 1d, 3d, and 5d of heat stress compared with the control treatment, GO and KEGG enrichment analysis revealed that some of the genes were highly enriched in oxidation-reduction process, response to stress, response to water deprivation, response to heat, carbon metabolism, protein processing in endoplasmic reticulum, and plant hormone signal transduction and may play vital roles in the heat stress response in jujube plants. Differentially expressed genes were identified in the two cultivars, including heat shock proteins, transcriptional factors, and ubiquitin-protein ligase genes. And the expression pattern of nine genes was also validated by qRT-PCR. These results will provide useful information for elucidating the molecular mechanism underlying heat stress in different jujube cultivars.

Two-dimensional fruit quality factors and soil nutrients reveals more favorable topographic plantation of Xinjiang jujubes in China.

Jujubes (Ziziphus jujuba Mill.) are among the main agroeconomically important crops in Xinjiang, China, and those from this region have the highest production worldwide. However, the reason for the high quality of the jujubes in the region is unknown. In our current research, the total phosphorus (P), total nitrogen (N), organic matter (OM), available P, alkaline N and quick potassium (K) were quantitatively analyzed in soils collected from orchards in 11 geographical locations, counties or cities, in Xinjiang. Meanwhile, the P, total triterpenoids, soluble solids, polysaccharide and cyclic adenosine monophosphate (CAMP) contents were also used to indicate fruit quality. Based on the analyzed data, principal component analysis (PCA) and multiple regression analysis revealed a high correlation between soil nutrients and the quality of the Jun jujube, which was used as an example. Specifically, the total P and quick K contents significantly differed among the orchard soils. Moreover, they significantly affected fruit quality. Total P significantly affected the soluble solids and total triterpenoids contents and was negatively correlated with the former and positively correlated with the latter. In addition, the soluble solids and total triterpenoids contents were significantly affected by the quick K content; as the quick K content increased, the soluble solids content gradually increased, while the total triterpenoids content decreased. According to the response surface model, we suggest that when the total P and quick K contents in the soil in Xinjiang were 0.76 g/kg and 365.04 mg/kg, respectively, the optimal fruit quality was obtained. Therefore, two-dimensional analysis of fruit quality and soil nutrients showed that it is necessary to increase the total P and quick K contents in the soil used to grow jujubes in Xinjiang.

Genome-wide analysis of the bHLH gene family in Chinese jujube (Ziziphus jujuba Mill.) and wild jujube.

BACKGROUND: The bHLH (basic helix-loop-helix) transcription factor is one of the largest families of transcription factors in plants, containing a large number of members with diverse functions. Chinese jujube (Ziziphus jujuba Mill.) is the species with the highest economic value in the family Rhamnaceae. However, the characteristics of the bHLH family in the jujube genome are still unclear. Hence, ZjbHLHs were first searched at a genome-wide level, their expression levels under various conditions were investigated systematically, and their protein-protein interaction networks were predicted. RESULTS: We identified 92 ZjbHLHs in the jujube genome, and these genes were classified into 16 classes according to bHLH domains. Ten ZjbHLHs with atypical bHLH domains were found. Seventy ZjbHLHs were mapped to but not evenly distributed on 12 pseudo- chromosomes. The domain sequences among ZjbHLHs were highly conserved, and their conserved residues were also identified. The tissue-specific expression of 37 ZjbHLH genes in jujube and wild jujube showed diverse patterns, revealing that these genes likely perform multiple functions. Many ZjbHLH genes were screened and found to be involved in flower and fruit development, especially in earlier developmental stages. A few genes responsive to phytoplasma invasion were also verified. Based on protein-protein interaction prediction and homology comparison, protein-protein interaction networks composed of 92 ZjbHLHs were also established. CONCLUSIONS: This study provides a comprehensive bioinformatics analysis of 92 identified ZjbHLH genes. We explored their expression patterns in various tissues, the flowering process, and fruit ripening and under phytoplasma stress. The protein-protein interaction networks of ZjbHLHs provide valuable clues toward further studies of their biological functions.

Effects of winter irrigation on soil salinity and jujube growth in arid regions.

The considerably high evapotranspiration and the low leaching fraction of the soil in arid regions are likely the primary causes of the enhanced soil salinity in such regions. Winter irrigation has proven to be very effective for promoting the leaching of salts from the rooting-zone. In this study, we investigated the effects of different irrigation methods (flood irrigation and drip irrigation) and winter irrigation quotas (450, 1350, 2250, 3150, 4050, and 4950 m3/hm2) on soil salinity and plant growth in an arid region. The sum of ECe in the 0-100 cm soil layer was 56.26-29.32 ms/cm under flood irrigation, 61.37-17.90 ms/cm under drip irrigation, and 64.13 ms/cm under no irrigation. The survival rates of jujube trees reached 65% and 77%, respectively, for drip irrigation and flood irrigation with a quota of 2250 m3/hm2. Furthermore, at irrigation quotas in excess of 3150 m3/hm2 the ground diameter and height of jujube trees were significantly greater than those observed under nonwinter irrigation and several other winter irrigation treatments. These findings indicated that winter irrigation significantly reduced soil salinity, changed the soil salt distribution, created a good environment for the growth of jujube trees and improved the survival rate of young jujube trees, especially under winter drip irrigation with a quota of 3150 m3/hm2. In addition, 1-year-old jujube trees emerging in spring may benefit from an ECe lower than 5 ms/cm.

Effects of intercropping with Bidens species plants on the growth and cadmium accumulation of Ziziphus acidojujuba seedlings.

To study the effects of intercropping with accumulator plants on heavy metal accumulation of fruit trees, plants of three Bidens species (Bidens pilosa, Bidens biternata, and Bidens parviflora) were intercropped with Ziziphus acidojujuba seedlings under cadmium (Cd)-contaminated conditions (5 mg kg-1). Intercropping with Bidens species increased the biomass and chlorophyll b content of Z. acidojujuba seedlings compared with monoculture, but decreased their carotenoid content. Intercropping with Bidens species also improved the activity of superoxide dismutase, peroxidase, and catalase in Z. acidojujuba seedlings compared with monoculture. Intercropping with Bidens species decreased the Cd content in the roots of Z. acidojujuba seedlings compared with monoculture. Conversely, when intercropped with B. pilosa, B. biternata, and B. parviflora, the Cd content in the shoots of Z. acidojujuba seedlings increased by 62.18%, 60.10%, and 62.18%, respectively, compared with that of those monocultured. When intercropped with Z. acidojujuba seedlings, the Cd accumulation amount of three Bidens species plants were ranked B. parviflora > B. biternata > B. pilosa. Therefore, intercropping with plants of three Bidens species is not suitable for Cd-contaminated jujube orchards.

Genome-Wide Identification of WRKY Transcription Factors in Chinese jujube (Ziziphus jujuba Mill.) and Their Involvement in Fruit Developing, Ripening, and Abiotic Stress.

Chinese jujube (Ziziphus jujuba Mill.) is an economically important fruit crop in China and mainly cultivated on land with high salinity and drought conditions in northern China. WRKY transcription factors (TFs) are involved in plant development and in responses to multiple abiotic stresses. In this study, we identified 61 and 52 putative ZjWRKY TFs in 'Junzao' and 'Dongzao' at the genome-wide level. Tissue expression profiling showed that 7 genes were constitutively expressed at high level in all tissues of 'Junzao'. Transcriptome analysis revealed that 39 ZjWRKY genes were expressed during 'Junzao' jujube fruit ripening. Among these genes, the transcript abundance of 19 genes were differentially expressed between 'Junzao' and 'Qingjiansuanzao' fruit. In addition, RT-qPCR analyses revealed that 30, 14, and 18 ZjWRKY genes responded to drought, NaCl, and ABA treatments, respectively. Taken together, ZjWRKY genes expression dynamics during jujube fruit development, ripening, and their differences between jujube and wild jujube would provide insights into their possible roles regulating fruit ripening. In addition, those ZjWRKY genes responded strongly to drought and salt stress, which provide candidate ZjWRKY genes for facilitating tolerance breeding.

Transcript analyses reveal a comprehensive role of abscisic acid in modulating fruit ripening in Chinese jujube.

BACKGROUND: Chinese jujube (Ziziphus jujuba Mill.) is a non-climacteric fruit; however, the underlying mechanism of ripening and the role of abscisic acid involved in this process are not yet understood for this species. RESULTS: In the present study, a positive correlation between dynamic changes in endogenous ABA and the onset of jujube ripening was determined. Transcript analyses suggested that the expression balance among genes encoding nine-cis-epoxycarotenoid dioxygenase (ZjNCED3), ABA-8'-hydroxylase (ZjCYP707A2), and beta-glucosidase (ZjBG4, ZjBG5, ZjBG8, and ZjBG9) has an important role in maintaining ABA accumulation, while the expression of a receptor (ZjPYL8), protein phosphatase 2C (ZjPP2C4-8), and sucrose nonfermenting 1-related protein kinase 2 (ZjSnRK2-2 and ZjSnRK2-5) is important in regulating fruit sensitivity to ABA applications. In addition, white mature 'Dongzao' fruit were harvested and treated with 50 mg L- 1 ABA or 50 mg L- 1 nordihydroguaiaretic acid (NDGA) to explore the role of ABA in jujube fruit ripening. By comparative transcriptome analyses, 1103 and 505 genes were differentially expressed in response to ABA and NDGA applications on the 1st day after treatment, respectively. These DEGs were associated with photosynthesis, secondary, lipid, cell wall, and starch and sugar metabolic processes, suggesting the involvement of ABA in modulating jujube fruit ripening. Moreover, ABA also exhibited crosstalk with other phytohormones and transcription factors, indicating a regulatory network for jujube fruit ripening. CONCLUSIONS: Our study further elucidated ABA-associated metabolic and regulatory processes. These findings are helpful for improving strategies for jujube fruit storage and for gaining insights into understand complex non-climacteric fruit ripening processes.

Intercropping With Fruit Trees Increases Population Abundance and Alters Species Composition of Spider Mites on Cotton.

With the recent increase in planting of fruit trees in southern Xinjiang, the intercropping of fruit trees and cotton has been widely adopted. From 2014 to 2016, a large-scale study was conducted in Aksu, an important agricultural area in southern Xinjiang, to compare the abundance and species composition of spider mites in cotton fields under jujube-cotton, apple-cotton, and cotton monocrop systems. The abundance of spider mites in cotton fields under both intercropping systems was generally higher than in the cotton monocrop. The species composition of spider mites also differed greatly between cotton intercropped with apple or jujube compared to the cotton monocrop. The relative proportion of Tetranychus truncates Ehara (Acari: Tetranychidae) in the species complex generally increased while that of another spider mite, Tetranychus dunhuangensis Wang (Acari: Tetranychidae), decreased under fruit tree-cotton systems. More attention should be paid to the monitoring and management of spider mites, especially T. truncates in this important region of China.

Transcript analyses of ethylene pathway genes during ripening of Chinese jujube fruit.

The fruit of Chinese jujube (Ziziphus jujuba Mill.) is immensely popular worldwide, while its fleshy fruit has a very short shelf life and suffers serious postharvest damage. The fruit has been controversially classified as non-climacteric, though the mechanisms underlying its ripening behavior, particularly the role of ethylene, have remained unclear. In this study, low and stable ethylene production was detected during ripening of Z. jujuba 'Dongzao' fruit, with production increasing at the full maturity stage. To determine potential ripening behavior, the fruit of five cultivars were harvested at the white mature stage, and all exhibited a first decreasing and then moderately increasing respiration rate without concomitant climacteric-like ethylene production during shelf storage. Treatment with 1.0 µL L-1 1-methylcyclopropene (1-MCP) inhibited respiration and ethylene production in white mature fruit, though the effects of 100 µL L-1 exogenous ethylene were not significant. The transcript levels of genes involved in ethylene biosynthesis, perception, and signal transduction were not elevated during fruit-ripening onset but substantially increased at the full-red ripening stage. Moreover, expression of genes controlling ethylene biosynthesis and perception mainly occurred in an auto-inhibited System-1-like manner, but signaling pathway genes were minimally affected by exogenous ethylene or 1-MCP. These results show that the ripening of Chinese jujube is non-climacteric. The basal level of ethylene likely plays a minor role in ripening regulation but is necessary to maintain normal ripening. This study elucidates the effects of ethylene on jujube fruit ripening, characterizing the ripening of this fruit as non-climacteric, and also provides strategies for the improvement and maintenance of fruit quality and the extension of shelf life during postharvest storage.

Transcriptome sequence analysis and mining of SSRs in Jhar Ber (Ziziphus nummularia (Burm.f.) Wight & Arn) under drought stress.

Ziziphus nummularia (Burm.f.) Wight & Arn., a perennial shrub that thrives in the arid regions, is naturally tolerant to drought. However, there are limited studies on the genomics of drought tolerance in Ziziphus sp. In this study, RNA-sequencing of one month old seedlings treated with PEG 6000 was performed using Roche GS-FLX454 Titanium pyrosequencing. A total of 367,176 raw sequence reads were generated, and upon adapter trimming and quality filtration 351,872 reads were assembled de novo into 32,739 unigenes. Further characterization of the unigenes indicated that 73.25% had significant hits in the protein database. Kyoto encyclopedia of genes and genomes database (KEGG) identified 113 metabolic pathways from the obtained unigenes. A large number of drought-responsive genes were obtained and among them differential gene expression of 16 highly induced genes was validated by qRT-PCR analysis. To develop genic-markers, 3,425 simple sequence repeats (SSRs) were identified in 2,813 unigene sequences. The data generated shall serve as an important reservoir for the identification and characterization of drought stress responsive genes for development of drought tolerant crops.

Genome-wide identification, characterization of the MADS-box gene family in Chinese jujube and their involvement in flower development.

MADS-box genes encode transcription factors that are involved in plant development control (particularly in floral organogenesis) and signal transduction pathways, though a comprehensive analysis of MADS-box family proteins in Chinese jujube (Ziziphus jujuba Mill.) is still missing. Here, we report a genome-wide analysis of the MADS-box gene family in Chinese jujube. Based on phylogenetic analyses, 52 jujube MADS-box genes were classified into 25 MIKCC-type, 3 MIKC*-type, 16 Mα, 5 Mß and 3 Mγ genes. 37 genes were randomly distributed across all 12 putative chromosomes. We found that the type II genes are more complex than the type I genes and that tandem duplications have occurred in three groups of MADS-box genes. Meanwhile, some gene pairs in the same clade displayed similar or distinct expression profiles, suggesting possible functional redundancy or divergence. MIKCC-type genes exhibited typical temporal and spatial expression patterns in the four whorls of floral tissues. The expressions of B, C/D and E-type genes were significantly suppressed in phyllody as compared to flower, providing valuable evidence for their involvement in flower development. This study is the first comprehensive analysis of the MADS-box family in jujube, and provides valuable information for elucidating molecular regulation mechanism of jujube flower development.

Ultrasound enhances calcium absorption of jujube fruit by regulating the cellular calcium distribution and metabolism of cell wall polysaccharides.

BACKGROUND: Ultrasound has been applied in fruit pre-washing processes. However, it is not sufficient to protect fruit from pathogenic infection throughout the entire storage period, and sometimes ultrasound causes tissue damage. The goal of this study was to investigate the effects of calcium chloride (CaCl2 , 10 g L-1 ) and ultrasound (350 W at 40 kHz), separately and in combination, on jujube fruit quality, antioxidant status, tissue Ca2+ content and distribution along with cell wall metabolism at 20 °C for 6 days. RESULTS: All three treatments significantly maintained fruit firmness and peel color, reduced respiration rate, decay incidence, superoxide anion, hydrogen peroxide and malondialdehyde and preserved higher enzymatic (superoxide dismutase, catalase and peroxidase) and non-enzymatic (ascorbic acid and glutathione) antioxidants compared with the control. Moreover, the combined treatment was more effective in increasing tissue Ca2+ content and distribution, inhibiting the generation of water-soluble and CDTA-soluble pectin fractions, delaying the solubilization of Na2 CO3 -soluble pectin and having lower activities of cell wall-modifying enzymes (polygalacturonase and pectate lyase) during storage. CONCLUSION: These results demonstrated that the combination of CaCl2 and ultrasound has potential commercial application to extend the shelf life of jujube fruit by facilitating Ca2+ absorption and stabilizing the cell wall structure. © 2017 Society of Chemical Industry.