The application of Compont gel in chronic obstructive jaundice rats model
Acta cir. bras
; 34(5): e201900504, 2019. tab, graf
Article
in En
| LILACS
| ID: biblio-1010871
Responsible library:
BR1.1
ABSTRACT
Abstract Purpose:
To establish a new rat model, the pathogenesis of which is closer to the clinical occurrence of chronic obstructive jaundice with liver fibrosis.Methods:
90 SD rats were randomly divided into 3 groups. Group A common bile duct ligation, group B common bile duct injection compont and group C injection saline. The serum of three groups was extracted, and the liver function was detected by ELISA. HE staining, Masson staining and immunohistochemistry were used to detect liver pathology.Results:
Group B showed a fluctuant development of jaundice, obstructive degree reached a peak at 2 weeks, and decreased from 3 weeks. HA, LA and PCIII were significantly higher than control group. 3 weeks after surgery, liver tissue fibrosis occurred in group B, and a wide range of fiber spacing was formed at 5 weeks. Immunohistochemistry showed that hepatic stellate cells were more active than the control group.Conclusion:
Intra-biliary injection of Compont gel is different from the classic obstructive jaundice animal model caused by classic bile duct ligation, which can provide an ideal rat model of chronic obstructive jaundice with liver fibrosis.Key words
Full text:
1
Collection:
01-internacional
Database:
LILACS
Main subject:
Bile Ducts
/
Disease Models, Animal
/
Gels
/
Liver Cirrhosis
Type of study:
Evaluation_studies
/
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Acta cir. bras
Journal subject:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Year:
2019
Document type:
Article
Affiliation country:
China
Country of publication:
Brazil