Urine as a promising sample for Leishmania DNA extraction in the diagnosis of visceral leishmaniasis - a review
Braz. j. infect. dis
; 23(2): 111-120, Mar.-Apr. 2019. tab
Article
in English
| LILACS
| ID: biblio-1011573
Responsible library:
BR1.1
ABSTRACT
ABSTRACT Visceral leishmaniasis is a serious and debilitating infection with high fatality rate in tropical and subtropical countries. As clinical symptoms of visceral leishmaniasis are not so specific, confirmatory diagnostic methods with high sensitivity and specificity are needed. Noninvasive methods have been developed using urine as a clinical sample for visceral leishmaniasis diagnosis. In fact, there is a clear correlation between kidney impairment and Leishmania DNA in urine. However, it has been proved that Leishmania nucleic acid may also be isolated from patients without any sign of renal involvement. Even though urine has become a promissing biological sample, it is still not widely used due to several issues, such as (i) incomprehension of the whole renal pathophysiology process in visceral leishmaniasis, (ii) presence of many amplification inhibitors in urine, and (iii) lack of an efficient urinary DNA extraction method. In this article, we performed a literature review to bring a new perspective for Leishmania DNA isolation in urine.
Full text:
Available
Collection:
International databases
Database:
LILACS
Main subject:
DNA, Protozoan
/
Leishmania
/
Leishmaniasis, Visceral
Type of study:
Diagnostic study
Limits:
Humans
Language:
English
Journal:
Braz. j. infect. dis
Journal subject:
Communicable Diseases
Year:
2019
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Fundação Oswaldo Cruz/BR
/
Universidade de Pernambuco/BR