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Could kDNA-PCR in peripheral blood replace the examination of bone marrow for the diagnosis of visceral leishmaniasis?
Godoy, Natália Souza de; Andrino, Marcos Luiz Alves; Souza, Regina Maia de; Gakiya, Erika; Amato, Valdir Sabbaga; Lindoso, José Ângelo Lauletta; Braz, Lucia Maria Almeida.
Affiliation
  • Godoy, Natália Souza de; Universidade de São Paulo. Faculdade de Mecicina. São Paulo. BR
  • Andrino, Marcos Luiz Alves; Universidade de São Paulo. Faculdade de Medicina. São Paulo. BR
  • Souza, Regina Maia de; Universidade de São Paulo. Faculdade de Medicina. São Paulo. BR
  • Gakiya, Erika; Universidade de São Paulo. Faculdade de Medicina. São Paulo. BR
  • Amato, Valdir Sabbaga; Universidade de São Paulo. Faculdade de Medicina. São Paulo. BR
  • Lindoso, José Ângelo Lauletta; Secretaria de Estado da Saúde. São Paulo. Instituto de Infectologia Emílio Ribas. São Paulo. BR
  • Braz, Lucia Maria Almeida; Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo. São Paulo. BR
J. parasitol. res ; : [1084353], Aug. 2016. tab
Article in English | Sec. Est. Saúde SP, SESSP-IIERPROD, Sec. Est. Saúde SP | ID: biblio-1021497
Responsible library: BR31.1
Localization: BR31.1; 2016_P-013
ABSTRACT
The aim of this study was to evaluate whether the molecular (kDNA-PCR) and parasitological diagnosis in peripheral blood (PB) could replace the invasive and painful bone marrow collection (BM) in the diagnosis of visceral leishmaniasis (VL). PB from suspected VL patients was evaluated by parasitological and molecular techniques using as the gold standard (GS) a combination of clinical, epidemiological, and immunochromatographic test (PB-rK39) results and parasitological examination of BM. Based on the GS, 38 samples from 32 patients were grouped Group 1, 20 samples of VL cases, and Group 2, 18 samples of non-VL cases. In order to evaluate the parasitological and molecular techniques in PB, the samples were examined. From Group 1, PB kDNA-PCR was positive in 20 samples and in 19 of 20 in BM kDNA-PCR examination. However, the parasitological examination of buffy coat was insensitive, being able to detect only 4 cases from Group 1. All samples from Group 2 were negative. We concluded that, for the diagnosis of visceral leishmaniasis, the parasitological examination of peripheral blood was not useful; however, molecular diagnosis by kDNA-PCR, performed in peripheral blood, could be useful to replace the parasitological examination of bone marrow
Subject(s)

Full text: Available Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IIERPROD Main subject: Bone Marrow Examination / Polymerase Chain Reaction / DNA, Kinetoplast / Leishmaniasis, Visceral Type of study: Diagnostic study Limits: Humans Language: English Journal: J. parasitol. res Year: 2016 Document type: Article Institution/Affiliation country: Secretaria de Estado da Saúde. São Paulo/BR / Universidade de São Paulo/BR

Full text: Available Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IIERPROD Main subject: Bone Marrow Examination / Polymerase Chain Reaction / DNA, Kinetoplast / Leishmaniasis, Visceral Type of study: Diagnostic study Limits: Humans Language: English Journal: J. parasitol. res Year: 2016 Document type: Article Institution/Affiliation country: Secretaria de Estado da Saúde. São Paulo/BR / Universidade de São Paulo/BR
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