Characterization of B-cell Responses to Zika Virus (ZIKV)
In. The University of the West Indies, Faculty of Medical Sciences. Faculty of Medical Sciences, Research Day. St. Augustine, Caribbean Medical Journal, March 21, 2019. .
Non-conventional
in English
| MedCarib
| ID: biblio-1022115
Responsible library:
TT5
ABSTRACT
Objective:
Most ZIKV infections occur in regions endemic for the related dengue virus (DENV). Anti-DENV antibodies have been demonstrated to cross-react with ZIKV. Some neutralize ZIKV infection while others mediate antibody-dependent enhancement (ADE), exacerbating ZIKV infection and complicating diagnosis of the etiologic agent. We aimed to characterize the humoral immune response in a ZIKV+, DENV- experienced individual in order to explore this anamnestic response and identify antibodies that may be useful in the development of therapeutic agents. Design andMethodology:
Peripheral blood mononuclear cells (PBMCs) were collected from an individual (TT66) who was newly infected with ZIKV but had two previous DENV infections. Plasmablasts were isolated and analyses conducted using Atreca's Immune Repertoire CaptureTM technology. Monoclonal antibodies (mAbs) derived from TT66 during their acute and convalescent phase of ZIKV infection were screened in vitro for ZIKV and DENV binding and neutralization activity. Epitopes were then mapped using a shotgun mutagenesis approach.Results:
We observed clonal expansion of two distinct antibody lineages representing 70% of total immunoglobulin sequences from TT66. We screened 18 mAbs representing two major lineages and five smaller families for neutralization and ADE between DENV and ZIKV. No highly typespecific mAbs were observed but rather a diverse pattern of neutralization, even within an individual lineage. Shotgun mutagenesis epitope mapping demonstrated epitopes for two of these broadly neutralizing mAb lineages lay within domain II ofE, close to the fusion loop.Conclusions:
Results suggest that neutralizing antibody responses to ZIKV are extensively shaped by previous DENV exposure.
Search on Google
Collection:
International databases
Health context:
Neglected Diseases
Health problem:
Dengue
Database:
MedCarib
Main subject:
Zika Virus
Limits:
Female
/
Humans
/
Male
Country/Region as subject:
English Caribbean
/
Trinidad and Tobago
Language:
English
Year:
2019
Document type:
Non-conventional
Institution/Affiliation country:
Atreca Inc/US
/
Integral Molecular/US
/
The University of the West Indies/TT