First isolation of cryptococcus neoformans genotype VNI MAT-alpha from wood hollow trunks of hymenaea courbaril
Med Mycol
; 54(1): 97-102, 2016. ilus, tab
Article
in En
| LILACS, SES-SP, SESSP-IALPROD, SES-SP, SESSP-IALACERVO
| ID: biblio-1022473
Responsible library:
BR91.2
Localization: BR76.1; P
ABSTRACT
Cryptococcal infection is transmitted by the inhalation of Cryptococcus spp. propagules. Information about the Cryptococcus species inhabiting plants might be clinically relevant due to the epidemiological role of these habitats as possible sources of human infection. The aim of this study was to increase the knowledge about the environmental occurrence of cryptococcosis agents. Hollow tree vegetal debris of nine plant species was sampled quarterly over a 12-month period. Melanized colonies were screened for Cryptococcus neoformans and C. gattii by biochemical tests, followed by URA5-RFLP molecular analysis, M13 fingerprinting assays, and mating-typing with the specific a and α primers. The susceptibility to fluconazole of all of the confirmed species colonies was determined using the AFST-EUCAST broth dilution method. We found that the typical Brazilian flora tree Hymenaea courbaril yielded a high cryptococcal burden (median, 10(2) CFU/g) during the summer, autumn and winter seasons. C. neoformans VNI molecular type MAT alpha was identified in all of the samples. The fingerprinting analyses showed great molecular variability with no correlation with the susceptibility profile to fluconazole (MIC range 4 to ≥64 mg/l). To our knowledge, this study is the first describing the association between C. neoformans and Hymenaea courbaril. These observations extend the known geographic distribution of and substantiate a new urban environmental niche for C. neoformans and also emphasize the genetic diversity of the environmental C. neoformans VNI molecular type isolates.
Full text:
1
Collection:
01-internacional
Database:
LILACS
/
SES-SP
/
SESSP-IALACERVO
/
SESSP-IALPROD
Main subject:
Seasons
/
Genetic Variation
/
Wood
/
Polymorphism, Restriction Fragment Length
/
Brazil
/
Colony Count, Microbial
/
Microbial Sensitivity Tests
/
Fluconazole
/
Mycological Typing Techniques
/
Cryptococcus neoformans
Country/Region as subject:
America do sul
/
Brasil
Language:
En
Journal:
Med Mycol
Year:
2016
Document type:
Article
Affiliation country:
Brazil