Human Cytomegalovirus DNA Quantification and Gene Expression in Gliomas of Different Grades
Plos one
; 11(7)2016.
Article
in English
| LILACS, Sec. Est. Saúde SP
| ID: biblio-1024785
Responsible library:
BR91.2
ABSTRACT
Gliomas are the most common type of primary brain tumors. The most aggressive type, Glioblastoma multiforme (GBM), is one of the deadliest human diseases, with an average survival at diagnosis of about 1 year. Previous evidence suggests a link between human cytomegalovirus (HCMV) and gliomas. HCMV has been shown to be present in these tumors and several viral proteins can have oncogenic properties in glioma cells. Here we have investigated the presence of HCMV DNA, RNA and proteins in fifty-two gliomas of different grades of malignancy. The UL83 viral region, the early beta 2.7 RNA and viral protein were detected in 73%, 36% and 57% by qPCR, ISH and IHC, respectively. Positivity of the viral targets and viral load was independent of tumor type or grade suggesting no correlation between viral presence and tumor progression. Our results demonstrate high prevalence of the virus in gliomas from Brazilian patients, contributing to a better understanding of the association between HCMV infection and gliomas worldwide and supporting further investigations of the virus oncomodulatory properties.
Full text:
Available
Collection:
National databases
/
Brazil
Database:
LILACS
/
Sec. Est. Saúde SP
Main subject:
Viral Proteins
/
Brazil
/
Humans
/
RNA, Viral
/
Immunohistochemistry
/
In Situ Hybridization
/
Cytomegalovirus Infections
/
Viral Load
/
Cytomegalovirus
/
Glioma
Type of study:
Risk factors
Country/Region as subject:
South America
/
Brazil
Language:
English
Journal:
Plos one
Year:
2016
Document type:
Article
Institution/Affiliation country:
Secretaria da Saúde do Estado de São Paulo/BR
/
Universidade Federal do ABC/BR
/
Universidade de São Paulo/BR