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Comparison of methodologies for detecting Trypanosoma cruzi parasites by microscopic observation of microhematocrit capillary tubes
Vera-Ku, Marina; Meza-González, Guillermo; Carlier, Yves; Truyens, Carine; Gamboa-León, Rubí.
Affiliation
  • Vera-Ku, Marina; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales "Dr. Hideyo Noguchi". Laboratorio de Parasitología. Mérida. MX
  • Meza-González, Guillermo; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales "Dr. Hideyo Noguchi". Laboratorio de Parasitología. Mérida. MX
  • Carlier, Yves; Université Libre de Bruxelles. Faculté de Médecine. Laboratoire de Parasitologie. Brussels. BE
  • Truyens, Carine; Université Libre de Bruxelles. Faculté de Médecine. Laboratoire de Parasitologie. Brussels. BE
  • Gamboa-León, Rubí; Universidad Autónoma de Yucatán. Centro de Investigaciones Regionales "Dr. Hideyo Noguchi". Laboratorio de Parasitología. Mérida. MX
Rev. Soc. Bras. Med. Trop ; 52: e20180505, 2019. tab, graf
Article in English | LILACS | ID: biblio-1041570
Responsible library: BR1.1
ABSTRACT
Abstract

INTRODUCTION:

The microscopic examination of microhematocrit tubes (mHCT) has been proposed as the gold standard for acute and congenital Chagas disease diagnosis. We compared different mHCT methodologies detecting T. cruzi parasites in the blood.

METHODS:

The rotating method, water mount, and immersion oil methods were compared for their suitability, sensitivity, and specificity.

RESULTS:

The rotating method was easier, faster, and more sensitive than the others with 100% specificity.

CONCLUSIONS:

The rotating method is feasible for laboratory technicians with standard training in microscopic techniques and is recommended for the diagnosis of acute Chagas disease in primary health care facilities.
Subject(s)

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Full text: Available Collection: International databases Health context: SDG3 - Health and Well-Being / Neglected Diseases / SDG3 - Target 3.3 End transmission of communicable diseases Health problem: Target 3.3: End transmission of communicable diseases / Chagas Disease / Neglected Diseases / Zoonoses / Chagas Disease / Sepsis Database: LILACS Main subject: Trypanosoma cruzi / Centrifugation / Chagas Disease / Parasitemia / Capillary Tubing / Hematocrit Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2019 Document type: Article Affiliation country: Belgium / Mexico Institution/Affiliation country: Universidad Autónoma de Yucatán/MX / Université Libre de Bruxelles/BE
Fulltext
Add to My VHL
Print
XML
Search on Google

Full text: Available Collection: International databases Health context: SDG3 - Health and Well-Being / Neglected Diseases / SDG3 - Target 3.3 End transmission of communicable diseases Health problem: Target 3.3: End transmission of communicable diseases / Chagas Disease / Neglected Diseases / Zoonoses / Chagas Disease / Sepsis Database: LILACS Main subject: Trypanosoma cruzi / Centrifugation / Chagas Disease / Parasitemia / Capillary Tubing / Hematocrit Type of study: Diagnostic study Limits: Animals / Humans Language: English Journal: Rev. Soc. Bras. Med. Trop Journal subject: Tropical Medicine Year: 2019 Document type: Article Affiliation country: Belgium / Mexico Institution/Affiliation country: Universidad Autónoma de Yucatán/MX / Université Libre de Bruxelles/BE