Quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus
Rev. bras. farmacogn
; 27(5): 645-649, Sept.-Oct. 2017. tab, graf
Article
in English
| LILACS
| ID: biblio-1042253
Responsible library:
BR1.1
ABSTRACT
Abstract This paper describes the quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus (Gomes) Landrum, Myrtaceae, citral chemotype using a validated HPLC-PDA method. The method employs a RP-18 column with acetonitrilewater-orthophosphoric acid 0.05% (gradient system) and UV detection at 210 nm. The method was demonstrated to be simple, sensitive, specific, linear, precise, accurate and robust. The response was linear over a range of 5-200 µg/ml (r > 0.999). The range of recoveries was 92.27-102.54%. The relative standard deviation values for intra- and inter-day precision studies were 4.30 and 3.78%, respectively. This assay can be readily utilized as quality control method for catechin in the dried extract of P. pseudocaryophyllus.
Full text:
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Collection:
International databases
Database:
LILACS
Language:
English
Journal:
Rev. bras. farmacogn
Journal subject:
Pharmacy
Year:
2017
Document type:
Article
Affiliation country:
Brazil
Institution/Affiliation country:
Universidade Estadual de Goiás/BR
/
Universidade Federal de Goiás/BR