Experimental model for removal of snake venom via hemoperfusion in rats
J Vet Emerg Crit Care
; 30(3): 286-294, Feb., 2020. graf., ilus.
Article
in English
| Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP
| ID: biblio-1052925
Responsible library:
BR79.1
Localization: BR79.1
ABSTRACT
OBJECTIVE:
To examine the efficiency of hemoperfusion in removing South American rattlesnake (Crotalus durissus terrificus) venom from rats compared with neutralization by antivenom.DESIGN:
An exploratory experimental investigation in rats involving the injection of snake venom with or without subsequent hemoperfusion or antivenom administration.SETTING:
Basic animal research laboratory in a private university. ANIMALS Normal, healthy male Wistar rats (0.29-0.40 kg, 3-6 months old) from a commercial breeder.INTERVENTIONS:
Four experimental groups of randomly allocated rats (n = 3/group) were studied Group 1 rats were injected with a single dose of venom (5 mg/kg, IM, in the right thigh) with no other treatment; blood samples were collected minutes before death to determine leukocyte, platelet, and erythrocyte counts; Group 2 (Control) rats underwent hemoperfusion alone for 60 min using a hemoperfusion cartridge designed for protein adsorption (by granulated charcoal) and protein precipitation (by tannic acid); Group 3 (Venom + antivenom) rats were injected with venom (5 mg/kg, IM) and, 10 min later, were treated with antivenom at the venomantivenom ratio recommended by the manufacturer; Group 4 (Venom + hemoperfusion) Rats were injected with venom (5 mg/kg, IM) and, 10 min later, were hemoperfused for 60 min. In groups 2-4, blood samples were collected for leukocyte, platelet, and erythrocyte counts 24 h after venom. MEASUREMENTS AND MAINRESULTS:
Rats injected with venom alone (Group 1) developed signs of neurotoxicity and ataxia and died in 9.0 ± 0.43 h but showed no changes in leukocyte or erythrocyte counts. In contrast, there were no deaths in groups 2-4. The lack of deaths in Groups 3 and 4 indicated that antivenom and hemoperfusion, respectively, protected against the lethal effects of the venom. ONCLUSIONS Hemoperfusion with a double-action hemoperfusion cartridge capable of protein adsorption and precipitation protected rats against C. d. terrificus venom. (AU)
Full text:
Available
Collection:
National databases
/
Brazil
Database:
Sec. Est. Saúde SP
/
SESSP-IDPCPROD
Main subject:
Hemoperfusion
/
Proanthocyanidins
/
Elapid Venoms
Limits:
Animals
Language:
English
Journal:
J Vet Emerg Crit Care
Year:
2020
Document type:
Article
Institution/Affiliation country:
Brazil University/BR
/
State University of Campinas (UNICAMP)/BR
/
University of Sorocaba (UNISO)/BR
/
University of São Paulo/BR