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Improved bioprocess with CHO-hTSH cells on higher microcarrier concentration provides higher overall biomass and productivity for rhtsh
C. Ventini, Daniella; P. B. Sousa, Alvaro; Damiani, Renata; Oliveira, João E. de; N. Peroni, Cibele; T. C. P. Ribela, Maria; Bartolini, Paolo; Tonso, Aldo; J. Soares, Carlos R; A. Pereira, Carlos.
Affiliation
  • C. Ventini, Daniella; Instituto Butantan. São Paulo. BR
  • P. B. Sousa, Alvaro; Instituto Butantan. São Paulo. BR
  • Damiani, Renata; s.af
  • Oliveira, João E. de; s.af
  • N. Peroni, Cibele; s.af
  • T. C. P. Ribela, Maria; s.af
  • Bartolini, Paolo; s.af
  • Tonso, Aldo; s.af
  • J. Soares, Carlos R; s.af
  • A. Pereira, Carlos; Instituto Butantan. São Paulo. BR
Applied Biochemistry and Biotechnology ; 164(4): 401-409, 2011.
Article in English | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP, SESSP-IBACERVO | ID: biblio-1059762
Responsible library: BR78.1
Localization: BR78.1
ABSTRACT
Since the recombinant thyroid-stimulating hormone (rhTSH) is secreted by stably transfected Chinese hamster ovary (CHO-hTSH) cells, a bioprocess consisting of immobilizing the cells on a substrate allowing their multiplication is very suitable for rhTSH recovering from supernatants at relative high degree of purity. In addition, such a system has also the advantage of easily allowing delicate manipulations of culture medium replacement. In the present study, we show the development of a laboratory scale bioprocess protocol of CHO-hTSH cell cultures on cytodex microcarriers (MCs) in a 1 L bioreactor, for the preparation of rhTSH batches in view of structure/function studies.
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Full text: Available Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Protein Biosynthesis / CHO Cells / Cell Culture Techniques / Thyrotrophs Limits: Animals Language: English Journal: Applied Biochemistry and Biotechnology Year: 2011 Document type: Article Institution/Affiliation country: Instituto Butantan/BR
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Full text: Available Collection: National databases / Brazil Database: Sec. Est. Saúde SP / SESSP-IBACERVO / SESSP-IBPROD Main subject: Protein Biosynthesis / CHO Cells / Cell Culture Techniques / Thyrotrophs Limits: Animals Language: English Journal: Applied Biochemistry and Biotechnology Year: 2011 Document type: Article Institution/Affiliation country: Instituto Butantan/BR